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Protein

Endoplasmic reticulum chaperone BiP homolog

Gene

hsp-4

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen (By similarity). Required for ER dynamics during the first embryonic cell divisions (PubMed:15716356). Specifically, controls ER transition into sheet-like structures at the onset of mitosis, possibly by regulating homotypic membrane fusion (PubMed:15716356).By similarity1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.By similarity

Activity regulationi

The chaperone activity is regulated by ATP-induced allosteric coupling of the nucleotide-binding (NBD) and substrate-binding (SBD) domains. In the ADP-bound and nucleotide-free (apo) states, the two domains have little interaction. In contrast, in the ATP-bound state the two domains are tightly coupled, which results in drastically accelerated kinetics in both binding and release of polypeptide substrates. J domain-containing co-chaperones stimulate the ATPase activity and are required for efficient substrate recognition.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 41ATPBy similarity4
Nucleotide bindingi229 – 231ATPBy similarity3
Nucleotide bindingi295 – 302ATPBy similarity8
Nucleotide bindingi366 – 369ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone, Hydrolase
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-3371453 Regulation of HSF1-mediated heat shock response

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmic reticulum chaperone BiP homologCurated
Alternative name(s):
Heat shock 70 kDa protein D (EC:3.6.4.10By similarity)
Gene namesi
Name:hsp-4
Synonyms:hsp70d
ORF Names:F43E2.8
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiF43E2.8a ; CE07244 ; WBGene00002008 ; hsp-4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown prevents the formation of ER sheet-like structures during mitosis in the 1-cell embryo. ER accumulates in foci throughout mitosis and partially fails to disperse at the end of mitosis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
ChainiPRO_000001354218 – 657Endoplasmic reticulum chaperone BiP homologAdd BLAST640

Proteomic databases

EPDiP20163
PaxDbiP20163
PeptideAtlasiP20163
PRIDEiP20163

2D gel databases

World-2DPAGEi0020:P20163

Expressioni

Inductioni

By endoplasmic reticulum stress.By similarity

Gene expression databases

BgeeiWBGene00002008 Expressed in 3 organ(s), highest expression level in multi-cellular organism

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi39538, 5 interactors
DIPiDIP-26704N
IntActiP20163, 3 interactors
STRINGi6239.F43E2.8.1

Structurei

Secondary structure

1657
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20163
SMRiP20163
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20163

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni128 – 282Nucleotide-binding (NBD)By similarityAdd BLAST155
Regioni402 – 502Substrate-binding (SBD)By similarityAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi654 – 657Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
GeneTreeiENSGT00930000151092
HOGENOMiHOG000228135
InParanoidiP20163
KOiK09490
OMAiDCRELGS
OrthoDBiEOG091G0352
PhylomeDBiP20163

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P20163-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKVFSLILIA FVANAYCDEG ASTEKEEKMG TIIGIDLGTT YSCVGVFKNG
60 70 80 90 100
RVEIIANDQG NRITPSYVAF SGDQGERLIG DAAKNQLTIN PENTIFDAKR
110 120 130 140 150
LIGRFYNEKT VQQDIKHWPF KIVDKSNKPN VEVKVGSETK QFTPEEVSAM
160 170 180 190 200
VLTKMKQIAE SYLGHEVKNA VVTVPAYFND AQKQATKDAG SIAGLNVVRI
210 220 230 240 250
INEPTAAAIA YGLDKKDGER NILVFDLGGG TFDVSLLTID SGVFEVLATN
260 270 280 290 300
GDTHLGGEDF DQRVMEYFIK LYKKKSGKDL RKDNRAVQKL RREVEKAKRA
310 320 330 340 350
LSTQHQTKIE IESLFDGEDF SETLTRAKFE ELNMDLFRAT LKPVQKVLED
360 370 380 390 400
ADMKKTDVHE IVLVGGSTRI PKVQQLIKDY FNGKEPSRGI NPDEAVAYGA
410 420 430 440 450
AVQAGVIGGV ENTGDVVLLD VNPLTLGIET VGGVMTKLIG RNTVIPTKKS
460 470 480 490 500
QVFSTAADSQ SAVSIVIYEG ERPMVMDNHK LGNFDVTGIP PAPRGVPQIE
510 520 530 540 550
VTFEIDVNGI LHVSAEDKGT GNKNKLTITN DHNRLSPEDI ERMINDADKF
560 570 580 590 600
AADDQAQKEK VESRNELEAY AYQMKTQIAD KEKLGGKLTD EDKVSIESAV
610 620 630 640 650
ERAIEWLGSN QDASTEENKE QKKELESVVQ PIVSKLYSAG GQGEQASEEP

SEDHDEL
Length:657
Mass (Da):72,288
Last modified:August 16, 2004 - v2
Checksum:i0C046D3EF1B5592F
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
V6CL98V6CL98_CAEEL
Heat shock 70 kDa protein D
hsp-4 CELE_F43E2.8, F43E2.8
324Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti454S → P in AAA28076 (PubMed:2744444).Curated1
Sequence conflicti574M → I in AAA28076 (PubMed:2744444).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080417 Genomic DNA Translation: CCD63548.1
M28528 Genomic DNA Translation: AAA28076.1
PIRiT34037
RefSeqiNP_495536.1, NM_063135.6
UniGeneiCel.38699

Genome annotation databases

EnsemblMetazoaiF43E2.8a.1; F43E2.8a.1; WBGene00002008
F43E2.8a.2; F43E2.8a.2; WBGene00002008
GeneIDi174203
KEGGicel:CELE_F43E2.8
UCSCiF43E2.8.1 c. elegans

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080417 Genomic DNA Translation: CCD63548.1
M28528 Genomic DNA Translation: AAA28076.1
PIRiT34037
RefSeqiNP_495536.1, NM_063135.6
UniGeneiCel.38699

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2OP6X-ray1.85A420-568[»]
ProteinModelPortaliP20163
SMRiP20163
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi39538, 5 interactors
DIPiDIP-26704N
IntActiP20163, 3 interactors
STRINGi6239.F43E2.8.1

2D gel databases

World-2DPAGEi0020:P20163

Proteomic databases

EPDiP20163
PaxDbiP20163
PeptideAtlasiP20163
PRIDEiP20163

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF43E2.8a.1; F43E2.8a.1; WBGene00002008
F43E2.8a.2; F43E2.8a.2; WBGene00002008
GeneIDi174203
KEGGicel:CELE_F43E2.8
UCSCiF43E2.8.1 c. elegans

Organism-specific databases

CTDi174203
WormBaseiF43E2.8a ; CE07244 ; WBGene00002008 ; hsp-4

Phylogenomic databases

eggNOGiKOG0101 Eukaryota
COG0443 LUCA
GeneTreeiENSGT00930000151092
HOGENOMiHOG000228135
InParanoidiP20163
KOiK09490
OMAiDCRELGS
OrthoDBiEOG091G0352
PhylomeDBiP20163

Enzyme and pathway databases

ReactomeiR-CEL-3371453 Regulation of HSF1-mediated heat shock response

Miscellaneous databases

EvolutionaryTraceiP20163
PROiPR:P20163

Gene expression databases

BgeeiWBGene00002008 Expressed in 3 organ(s), highest expression level in multi-cellular organism

Family and domain databases

Gene3Di1.20.1270.10, 1 hit
2.60.34.10, 1 hit
InterProiView protein in InterPro
IPR018181 Heat_shock_70_CS
IPR029048 HSP70_C_sf
IPR029047 HSP70_peptide-bd_sf
IPR013126 Hsp_70_fam
PANTHERiPTHR19375 PTHR19375, 1 hit
PfamiView protein in Pfam
PF00012 HSP70, 1 hit
PRINTSiPR00301 HEATSHOCK70
SUPFAMiSSF100920 SSF100920, 1 hit
SSF100934 SSF100934, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit
PS00297 HSP70_1, 1 hit
PS00329 HSP70_2, 1 hit
PS01036 HSP70_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiBIBH_CAEEL
AccessioniPrimary (citable) accession number: P20163
Secondary accession number(s): O02085
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: August 16, 2004
Last modified: November 7, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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