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Protein

Azurocidin

Gene

AZU1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.3 Publications

GO - Molecular functioni

  • heparan sulfate proteoglycan binding Source: UniProtKB
  • heparin binding Source: UniProtKB
  • peptidase activity Source: UniProtKB
  • serine-type endopeptidase activity Source: InterPro
  • toxic substance binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Heparin-binding, Serine protease homolog
Biological processChemotaxis

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Protein family/group databases

MEROPSiS01.971

Names & Taxonomyi

Protein namesi
Recommended name:
Azurocidin1 Publication
Alternative name(s):
Cationic antimicrobial protein CAP371 Publication
Heparin-binding protein1 Publication
Short name:
HBP1 Publication
Short name:
hHBP1 Publication
Gene namesi
Name:AZU1Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000172232.9
HGNCiHGNC:913 AZU1
MIMi162815 gene
neXtProtiNX_P20160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi52C → S: Loss of antibiotic activity. 1 Publication1
Mutagenesisi68C → S: Loss of antibiotic activity. 1 Publication1

Organism-specific databases

DisGeNETi566
OpenTargetsiENSG00000172232
PharmGKBiPA25206

Polymorphism and mutation databases

BioMutaiAZU1
DMDMi416746

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 191 PublicationAdd BLAST19
PropeptideiPRO_000043537220 – 26Removed in mature form10 Publications7
PropeptideiPRO_000043537325 – 26Dipeptide found in non-mature form1 Publication2
ChainiPRO_000002770527 – 248Azurocidin2 PublicationsAdd BLAST222
PropeptideiPRO_0000027706249 – 251Removed in mature form2 Publications3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi52 ↔ 682 Publications
Glycosylationi126N-linked (GlcNAc...) asparagine; partial2 Publications1
Glycosylationi140N-linked (GlcNAc...) asparagine2 Publications1
Disulfide bondi149 ↔ 2072 Publications
Glycosylationi171N-linked (GlcNAc...) asparagine; partial2 Publications1
Disulfide bondi180 ↔ 1862 Publications
Disulfide bondi197 ↔ 2222 Publications

Post-translational modificationi

Cleavage of the N-terminal propeptide which is composed of 7 amino acids occurs in two steps. The initial cleavage of 5 amino acids is followed by the cleavage of a dipeptide to produce the mature form.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP20160
PaxDbiP20160
PeptideAtlasiP20160
PRIDEiP20160
ProteomicsDBi53732

PTM databases

GlyConnecti1023
iPTMnetiP20160

Expressioni

Gene expression databases

BgeeiENSG00000172232 Expressed in 80 organ(s), highest expression level in trabecular bone tissue
CleanExiHS_AZU1
ExpressionAtlasiP20160 baseline and differential
GenevisibleiP20160 HS

Organism-specific databases

HPAiHPA055851

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107043, 14 interactors
IntActiP20160, 2 interactors
MINTiP20160
STRINGi9606.ENSP00000233997

Structurei

Secondary structure

1251
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP20160
SMRiP20160
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP20160

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 244Peptidase S1PROSITE-ProRule annotationAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 70Possesses antibiotic activity1 PublicationAdd BLAST25

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00910000144219
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP20160
OMAiALFRNWI
OrthoDBiEOG091G0DF7
PhylomeDBiP20160
TreeFamiTF335284

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P20160-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTRLTVLALL AGLLASSRAG SSPLLDIVGG RKARPRQFPF LASIQNQGRH
60 70 80 90 100
FCGGALIHAR FVMTAASCFQ SQNPGVSTVV LGAYDLRRRE RQSRQTFSIS
110 120 130 140 150
SMSENGYDPQ QNLNDLMLLQ LDREANLTSS VTILPLPLQN ATVEAGTRCQ
160 170 180 190 200
VAGWGSQRSG GRLSRFPRFV NVTVTPEDQC RPNNVCTGVL TRRGGICNGD
210 220 230 240 250
GGTPLVCEGL AHGVASFSLG PCGRGPDFFT RVALFRDWID GVLNNPGPGP

A
Length:251
Mass (Da):26,886
Last modified:October 1, 1993 - v3
Checksum:i22F80D9EBE87DE60
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WXP0A0A087WXP0_HUMAN
Azurocidin
AZU1
150Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti36R → H AA sequence (PubMed:2404977).Curated1
Sequence conflicti130S → N AA sequence (PubMed:2026172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96326 Genomic DNA Translation: AAB59353.1
AC004799 Genomic DNA No translation available.
BC069495 mRNA Translation: AAH69495.1
BC093931 mRNA Translation: AAH93931.1
BC093933 mRNA Translation: AAH93933.1
X58794 mRNA Translation: CAA41601.1
CCDSiCCDS12044.1
PIRiA46268 TRHUAZ
RefSeqiNP_001691.1, NM_001700.4
UniGeneiHs.72885

Genome annotation databases

EnsembliENST00000233997; ENSP00000233997; ENSG00000172232
ENST00000620695; ENSP00000479183; ENSG00000278624
GeneIDi566
KEGGihsa:566
UCSCiuc002lpz.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96326 Genomic DNA Translation: AAB59353.1
AC004799 Genomic DNA No translation available.
BC069495 mRNA Translation: AAH69495.1
BC093931 mRNA Translation: AAH93931.1
BC093933 mRNA Translation: AAH93933.1
X58794 mRNA Translation: CAA41601.1
CCDSiCCDS12044.1
PIRiA46268 TRHUAZ
RefSeqiNP_001691.1, NM_001700.4
UniGeneiHs.72885

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A7SX-ray1.12A27-251[»]
1AE5X-ray2.30A27-251[»]
1FY1X-ray2.50A27-251[»]
1FY3X-ray1.89A27-251[»]
ProteinModelPortaliP20160
SMRiP20160
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107043, 14 interactors
IntActiP20160, 2 interactors
MINTiP20160
STRINGi9606.ENSP00000233997

Protein family/group databases

MEROPSiS01.971

PTM databases

GlyConnecti1023
iPTMnetiP20160

Polymorphism and mutation databases

BioMutaiAZU1
DMDMi416746

Proteomic databases

EPDiP20160
PaxDbiP20160
PeptideAtlasiP20160
PRIDEiP20160
ProteomicsDBi53732

Protocols and materials databases

DNASUi566
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000233997; ENSP00000233997; ENSG00000172232
ENST00000620695; ENSP00000479183; ENSG00000278624
GeneIDi566
KEGGihsa:566
UCSCiuc002lpz.2 human

Organism-specific databases

CTDi566
DisGeNETi566
EuPathDBiHostDB:ENSG00000172232.9
GeneCardsiAZU1
HGNCiHGNC:913 AZU1
HPAiHPA055851
MIMi162815 gene
neXtProtiNX_P20160
OpenTargetsiENSG00000172232
PharmGKBiPA25206
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00910000144219
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiP20160
OMAiALFRNWI
OrthoDBiEOG091G0DF7
PhylomeDBiP20160
TreeFamiTF335284

Enzyme and pathway databases

ReactomeiR-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

EvolutionaryTraceiP20160
GeneWikiiAzurocidin_1
GenomeRNAii566
PROiPR:P20160
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172232 Expressed in 80 organ(s), highest expression level in trabecular bone tissue
CleanExiHS_AZU1
ExpressionAtlasiP20160 baseline and differential
GenevisibleiP20160 HS

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAP7_HUMAN
AccessioniPrimary (citable) accession number: P20160
Secondary accession number(s): P80014
, Q52LG4, Q9UCM1, Q9UCT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1993
Last modified: November 7, 2018
This is version 185 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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