UniProtKB - P20160 (CAP7_HUMAN)
Protein
Azurocidin
Gene
AZU1
Organism
Homo sapiens (Human)
Status
Functioni
This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G.3 Publications
GO - Molecular functioni
- heparan sulfate proteoglycan binding Source: UniProtKB
- heparin binding Source: UniProtKB
- peptidase activity Source: UniProtKB
- serine-type endopeptidase activity Source: InterPro
- toxic substance binding Source: UniProtKB
GO - Biological processi
- antimicrobial humoral response Source: UniProtKB
- calcium-mediated signaling using intracellular calcium source Source: UniProtKB
- cell chemotaxis Source: UniProtKB
- cellular extravasation Source: UniProtKB
- defense response to Gram-negative bacterium Source: UniProtKB
- defense response to virus Source: UniProtKB
- fractalkine production Source: UniProtKB
- glial cell migration Source: UniProtKB
- induction of positive chemotaxis Source: UniProtKB
- inflammatory response Source: UniProtKB
- macrophage chemotaxis Source: UniProtKB
- microglial cell activation Source: UniProtKB
- monocyte activation Source: UniProtKB
- negative regulation of apoptotic process Source: UniProtKB
- neutrophil degranulation Source: Reactome
- neutrophil-mediated killing of bacterium Source: UniProtKB
- positive regulation of cell adhesion Source: UniProtKB
- positive regulation of gene expression Source: UniProtKB
- positive regulation of interleukin-1 beta production Source: UniProtKB
- positive regulation of MHC class II biosynthetic process Source: UniProtKB
- positive regulation of peptidyl-threonine phosphorylation Source: UniProtKB
- positive regulation of phagocytosis Source: UniProtKB
- positive regulation of protein kinase activity Source: UniProtKB
- positive regulation of tumor necrosis factor biosynthetic process Source: UniProtKB
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- protein kinase C signaling Source: UniProtKB
- proteolysis Source: UniProtKB
- regulation of vascular permeability Source: UniProtKB
Keywordsi
Molecular function | Antibiotic, Antimicrobial, Heparin-binding, Serine protease homolog |
Biological process | Chemotaxis |
Enzyme and pathway databases
PathwayCommonsi | P20160 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Protein family/group databases
MEROPSi | S01.971 |
Names & Taxonomyi
Protein namesi | Recommended name: Azurocidin1 PublicationAlternative name(s): Cationic antimicrobial protein CAP371 Publication Heparin-binding protein1 Publication Short name: HBP1 Publication Short name: hHBP1 Publication |
Gene namesi | Name:AZU1Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000172232.9 |
HGNCi | HGNC:913, AZU1 |
MIMi | 162815, gene |
neXtProti | NX_P20160 |
Subcellular locationi
Other locations
- Cytoplasmic granule membrane 2 Publications; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Localizes to azurophil granules of neutrophil granulocytes. Also called primary granules, these specialized lysosomes of the neutrophil formed early during promyelocyte development store antibacterial proteins and peptides.2 Publications
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
- extracellular space Source: UniProtKB
Lysosome
- azurophil granule Source: UniProtKB
- azurophil granule lumen Source: Reactome
- azurophil granule membrane Source: UniProtKB
Other locations
- extrinsic component of membrane Source: UniProtKB
- intracellular membrane-bounded organelle Source: HPA
Keywords - Cellular componenti
MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 | C → S: Loss of antibiotic activity. 1 Publication | 1 | |
Mutagenesisi | 68 | C → S: Loss of antibiotic activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 566 |
OpenTargetsi | ENSG00000172232 |
PharmGKBi | PA25206 |
Miscellaneous databases
Pharosi | P20160, Tbio |
Polymorphism and mutation databases
BioMutai | AZU1 |
DMDMi | 416746 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | 1 PublicationAdd BLAST | 19 | |
PropeptideiPRO_0000435372 | 20 – 26 | Removed in mature form10 Publications | 7 | |
PropeptideiPRO_0000435373 | 25 – 26 | Dipeptide found in non-mature form1 Publication | 2 | |
ChainiPRO_0000027705 | 27 – 248 | Azurocidin2 PublicationsAdd BLAST | 222 | |
PropeptideiPRO_0000027706 | 249 – 251 | Removed in mature form2 Publications | 3 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 52 ↔ 68 | 2 Publications | ||
Glycosylationi | 126 | N-linked (GlcNAc...) asparagine; partial2 Publications | 1 | |
Glycosylationi | 140 | N-linked (GlcNAc...) asparagine2 Publications | 1 | |
Disulfide bondi | 149 ↔ 207 | 2 Publications | ||
Glycosylationi | 171 | N-linked (GlcNAc...) asparagine; partial2 Publications | 1 | |
Disulfide bondi | 180 ↔ 186 | 2 Publications | ||
Disulfide bondi | 197 ↔ 222 | 2 Publications |
Post-translational modificationi
Cleavage of the N-terminal propeptide which is composed of 7 amino acids occurs in two steps. The initial cleavage of 5 amino acids is followed by the cleavage of a dipeptide to produce the mature form.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
EPDi | P20160 |
jPOSTi | P20160 |
MassIVEi | P20160 |
PaxDbi | P20160 |
PeptideAtlasi | P20160 |
PRIDEi | P20160 |
ProteomicsDBi | 53732 |
PTM databases
GlyConnecti | 1023, 3 N-Linked glycans (1 site) |
GlyGeni | P20160, 3 sites |
iPTMneti | P20160 |
Expressioni
Gene expression databases
Bgeei | ENSG00000172232, Expressed in bone marrow and 104 other tissues |
ExpressionAtlasi | P20160, baseline and differential |
Genevisiblei | P20160, HS |
Organism-specific databases
HPAi | ENSG00000172232, Tissue enriched (bone) |
Interactioni
GO - Molecular functioni
- heparan sulfate proteoglycan binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 107043, 15 interactors |
IntActi | P20160, 14 interactors |
MINTi | P20160 |
STRINGi | 9606.ENSP00000233997 |
Miscellaneous databases
RNActi | P20160, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P20160 |
SMRi | P20160 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20160 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 244 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 218 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 46 – 70 | Possesses antibiotic activity1 PublicationAdd BLAST | 25 |
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203560 |
HOGENOMi | CLU_006842_1_0_1 |
InParanoidi | P20160 |
OMAi | WILTAKH |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P20160 |
TreeFami | TF335284 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P20160-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTRLTVLALL AGLLASSRAG SSPLLDIVGG RKARPRQFPF LASIQNQGRH
60 70 80 90 100
FCGGALIHAR FVMTAASCFQ SQNPGVSTVV LGAYDLRRRE RQSRQTFSIS
110 120 130 140 150
SMSENGYDPQ QNLNDLMLLQ LDREANLTSS VTILPLPLQN ATVEAGTRCQ
160 170 180 190 200
VAGWGSQRSG GRLSRFPRFV NVTVTPEDQC RPNNVCTGVL TRRGGICNGD
210 220 230 240 250
GGTPLVCEGL AHGVASFSLG PCGRGPDFFT RVALFRDWID GVLNNPGPGP
A
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A087WXP0 | A0A087WXP0_HUMAN | Azurocidin | AZU1 | 150 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 36 | R → H AA sequence (PubMed:2404977).Curated | 1 | |
Sequence conflicti | 130 | S → N AA sequence (PubMed:2026172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96326 Genomic DNA Translation: AAB59353.1 AC004799 Genomic DNA No translation available. BC069495 mRNA Translation: AAH69495.1 BC093931 mRNA Translation: AAH93931.1 BC093933 mRNA Translation: AAH93933.1 X58794 mRNA Translation: CAA41601.1 |
CCDSi | CCDS12044.1 |
PIRi | A46268, TRHUAZ |
RefSeqi | NP_001691.1, NM_001700.4 |
Genome annotation databases
Ensembli | ENST00000233997; ENSP00000233997; ENSG00000172232 ENST00000620695; ENSP00000479183; ENSG00000278624 |
GeneIDi | 566 |
KEGGi | hsa:566 |
UCSCi | uc002lpz.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M96326 Genomic DNA Translation: AAB59353.1 AC004799 Genomic DNA No translation available. BC069495 mRNA Translation: AAH69495.1 BC093931 mRNA Translation: AAH93931.1 BC093933 mRNA Translation: AAH93933.1 X58794 mRNA Translation: CAA41601.1 |
CCDSi | CCDS12044.1 |
PIRi | A46268, TRHUAZ |
RefSeqi | NP_001691.1, NM_001700.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A7S | X-ray | 1.12 | A | 27-251 | [»] | |
1AE5 | X-ray | 2.30 | A | 27-251 | [»] | |
1FY1 | X-ray | 2.50 | A | 27-251 | [»] | |
1FY3 | X-ray | 1.89 | A | 27-251 | [»] | |
BMRBi | P20160 | |||||
SMRi | P20160 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107043, 15 interactors |
IntActi | P20160, 14 interactors |
MINTi | P20160 |
STRINGi | 9606.ENSP00000233997 |
Protein family/group databases
MEROPSi | S01.971 |
PTM databases
GlyConnecti | 1023, 3 N-Linked glycans (1 site) |
GlyGeni | P20160, 3 sites |
iPTMneti | P20160 |
Polymorphism and mutation databases
BioMutai | AZU1 |
DMDMi | 416746 |
Proteomic databases
EPDi | P20160 |
jPOSTi | P20160 |
MassIVEi | P20160 |
PaxDbi | P20160 |
PeptideAtlasi | P20160 |
PRIDEi | P20160 |
ProteomicsDBi | 53732 |
Protocols and materials databases
Antibodypediai | 22412, 295 antibodies |
DNASUi | 566 |
Genome annotation databases
Ensembli | ENST00000233997; ENSP00000233997; ENSG00000172232 ENST00000620695; ENSP00000479183; ENSG00000278624 |
GeneIDi | 566 |
KEGGi | hsa:566 |
UCSCi | uc002lpz.2, human |
Organism-specific databases
CTDi | 566 |
DisGeNETi | 566 |
EuPathDBi | HostDB:ENSG00000172232.9 |
GeneCardsi | AZU1 |
HGNCi | HGNC:913, AZU1 |
HPAi | ENSG00000172232, Tissue enriched (bone) |
MIMi | 162815, gene |
neXtProti | NX_P20160 |
OpenTargetsi | ENSG00000172232 |
PharmGKBi | PA25206 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00990000203560 |
HOGENOMi | CLU_006842_1_0_1 |
InParanoidi | P20160 |
OMAi | WILTAKH |
OrthoDBi | 1314811at2759 |
PhylomeDBi | P20160 |
TreeFami | TF335284 |
Enzyme and pathway databases
PathwayCommonsi | P20160 |
Reactomei | R-HSA-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 566, 0 hits in 842 CRISPR screens |
EvolutionaryTracei | P20160 |
GeneWikii | Azurocidin_1 |
GenomeRNAii | 566 |
Pharosi | P20160, Tbio |
PROi | PR:P20160 |
RNActi | P20160, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000172232, Expressed in bone marrow and 104 other tissues |
ExpressionAtlasi | P20160, baseline and differential |
Genevisiblei | P20160, HS |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CAP7_HUMAN | |
Accessioni | P20160Primary (citable) accession number: P20160 Secondary accession number(s): P80014 Q9UCT5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | October 1, 1993 | |
Last modified: | December 2, 2020 | |
This is version 200 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Peptidase families
Classification of peptidase families and list of entries