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UniProtKB - P20153 (USP_DROME)
Protein
Protein ultraspiracle
Gene
usp
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Receptor for ecdysone. May be an important modulator of insect metamorphosis. Plays an important part in embryonic and post-embryonic development. Binds to ecdysone response elements (ECRES) such as in the promoter region of s15 chorion gene.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 104 – 169 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 66 | |
Zinc fingeri | 104 – 124 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 140 – 164 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- core promoter sequence-specific DNA binding Source: CAFA
- DNA binding Source: FlyBase
- hormone binding Source: FlyBase
- lipid binding Source: FlyBase
- nuclear receptor activity Source: FlyBase
- protein heterodimerization activity Source: CAFA
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- signaling receptor binding Source: CAFA
- steroid hormone receptor activity Source: InterPro
- transcription cis-regulatory region binding Source: FlyBase
- zinc ion binding Source: InterPro
GO - Biological processi
- anatomical structure development Source: GO_Central
- border follicle cell migration Source: FlyBase
- cell differentiation Source: GO_Central
- cellular response to ecdysone Source: FlyBase
- dendrite morphogenesis Source: FlyBase
- ecdysone biosynthetic process Source: FlyBase
- germ cell development Source: FlyBase
- metamorphosis Source: CACAO
- mushroom body development Source: FlyBase
- negative regulation of cell differentiation Source: FlyBase
- negative regulation of transcription, DNA-templated Source: FlyBase
- neuron remodeling Source: FlyBase
- positive regulation of transcription, DNA-templated Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: GO_Central
- pupariation Source: FlyBase
- regulation of development, heterochronic Source: FlyBase
- regulation of organ growth Source: FlyBase
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to ecdysone Source: FlyBase
- response to starvation Source: FlyBase
Keywordsi
Molecular function | DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DME-159418, Recycling of bile acids and salts R-DME-200425, Carnitine metabolism R-DME-204174, Regulation of pyruvate dehydrogenase (PDH) complex R-DME-381340, Transcriptional regulation of white adipocyte differentiation R-DME-383280, Nuclear Receptor transcription pathway R-DME-400206, Regulation of lipid metabolism by PPARalpha R-DME-5362517, Signaling by Retinoic Acid R-DME-9029569, NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux R-DME-9616222, Transcriptional regulation of granulopoiesis R-DME-9623433, NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis R-DME-9707564, Cytoprotection by HMOX1 |
SignaLinki | P20153 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein ultraspiracleAlternative name(s): Chorion factor 1 Nuclear receptor subfamily 2 group B member 4 XR2C |
Gene namesi | Name:usp Synonyms:Cf1, NR2B4 ORF Names:CG4380 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003964, usp |
VEuPathDBi | VectorBase:FBgn0003964 |
Subcellular locationi
Nucleus
Nucleus
- activator ecdysone receptor complex Source: FlyBase
- ecdysone receptor holocomplex Source: CAFA
- nucleus Source: FlyBase
- RNA polymerase II transcription regulator complex Source: GO_Central
Other locations
- dendrite Source: FlyBase
- polytene chromosome Source: FlyBase
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053582 | 1 – 508 | Protein ultraspiracleAdd BLAST | 508 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 35 | Phosphoserine1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P20153 |
PRIDEi | P20153 |
PTM databases
iPTMneti | P20153 |
Expressioni
Gene expression databases
Bgeei | FBgn0003964, Expressed in cleaving embryo and 42 other tissues |
ExpressionAtlasi | P20153, baseline and differential |
Genevisiblei | P20153, DM |
Interactioni
Subunit structurei
Heterodimer of USP and ECR. Only the heterodimer is capable of high-affinity binding to ecdysone.
1 PublicationGO - Molecular functioni
- protein heterodimerization activity Source: CAFA
- signaling receptor binding Source: CAFA
Protein-protein interaction databases
BioGRIDi | 57709, 35 interactors |
DIPi | DIP-645N |
IntActi | P20153, 3 interactors |
STRINGi | 7227.FBpp0305937 |
Chemistry databases
BindingDBi | P20153 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P20153 |
SMRi | P20153 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20153 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 239 – 498 | NR LBDPROSITE-ProRule annotationAdd BLAST | 260 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 103 | ModulatingBy similarityAdd BLAST | 103 | |
Regioni | 21 – 40 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 55 – 92 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 170 – 223 | HingeAdd BLAST | 54 | |
Regioni | 178 – 228 | DisorderedSequence analysisAdd BLAST | 51 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 24 – 39 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 190 – 212 | Polar residuesSequence analysisAdd BLAST | 23 |
Domaini
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 104 – 124 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 140 – 164 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000170897 |
HOGENOMi | CLU_007368_5_0_1 |
InParanoidi | P20153 |
OMAi | DEHCRQE |
OrthoDBi | 912470at2759 |
PhylomeDBi | P20153 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50250 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR000003, Retinoid-X_rcpt/HNF4 IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00545, RETINOIDXR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P20153-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDNCDQDASF RLSHIKEEVK PDISQLNDSN NSSFSPKAES PVPFMQAMSM
60 70 80 90 100
VHVLPGSNSA SSNNNSAGDA QMAQAPNSAG GSAAAAVQQQ YPPNHPLSGS
110 120 130 140 150
KHLCSICGDR ASGKHYGVYS CEGCKGFFKR TVRKDLTYAC RENRNCIIDK
160 170 180 190 200
RQRNRCQYCR YQKCLTCGMK REAVQEERQR GARNAAGRLS ASGGGSSGPG
210 220 230 240 250
SVGGSSSQGG GGGGGVSGGM GSGNGSDDFM TNSVSRDFSI ERIIEAEQRA
260 270 280 290 300
ETQCGDRALT FLRVGPYSTV QPDYKGAVSA LCQVVNKQLF QMVEYARMMP
310 320 330 340 350
HFAQVPLDDQ VILLKAAWIE LLIANVAWCS IVSLDDGGAG GGGGGLGHDG
360 370 380 390 400
SFERRSPGLQ PQQLFLNQSF SYHRNSAIKA GVSAIFDRIL SELSVKMKRL
410 420 430 440 450
NLDRRELSCL KAIILYNPDI RGIKSRAEIE MCREKVYACL DEHCRLEHPG
460 470 480 490 500
DDGRFAQLLL RLPALRSISL KCQDHLFLFR ITSDRPLEEL FLEQLEAPPP
PGLAMKLE
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM9PGS2 | M9PGS2_DROME | Ultraspiracle, isoform B | usp 2C, CF1, Cf1, cf1, Dmel\CG4380 | 508 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 213 | G → E in CAA36827 (PubMed:2165589).Curated | 1 | |
Sequence conflicti | 339 | Missing in CAA36827 (PubMed:2165589).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53417 mRNA Translation: CAA37496.1 X52591 mRNA Translation: CAA36827.1 AE014298 Genomic DNA Translation: AAF45707.1 AL031765 Genomic DNA Translation: CAA21122.1 AY069393 mRNA Translation: AAL39538.1 X53379 mRNA Translation: CAA37459.1 |
PIRi | A35872 T13737 |
RefSeqi | NP_001259168.1, NM_001272239.2 NP_476781.1, NM_057433.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0070346; FBpp0070332; FBgn0003964 FBtr0333803; FBpp0305937; FBgn0003964 |
GeneIDi | 31165 |
KEGGi | dme:Dmel_CG4380 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53417 mRNA Translation: CAA37496.1 X52591 mRNA Translation: CAA36827.1 AE014298 Genomic DNA Translation: AAF45707.1 AL031765 Genomic DNA Translation: CAA21122.1 AY069393 mRNA Translation: AAL39538.1 X53379 mRNA Translation: CAA37459.1 |
PIRi | A35872 T13737 |
RefSeqi | NP_001259168.1, NM_001272239.2 NP_476781.1, NM_057433.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HG4 | X-ray | 2.40 | A/B/C/D/E/F | 230-508 | [»] | |
1R0O | X-ray | 2.24 | A | 94-179 | [»] | |
2HAN | X-ray | 1.95 | A | 94-179 | [»] | |
AlphaFoldDBi | P20153 | |||||
SMRi | P20153 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 57709, 35 interactors |
DIPi | DIP-645N |
IntActi | P20153, 3 interactors |
STRINGi | 7227.FBpp0305937 |
Chemistry databases
BindingDBi | P20153 |
ChEMBLi | CHEMBL2366581 |
PTM databases
iPTMneti | P20153 |
Proteomic databases
PaxDbi | P20153 |
PRIDEi | P20153 |
Genome annotation databases
EnsemblMetazoai | FBtr0070346; FBpp0070332; FBgn0003964 FBtr0333803; FBpp0305937; FBgn0003964 |
GeneIDi | 31165 |
KEGGi | dme:Dmel_CG4380 |
Organism-specific databases
CTDi | 31165 |
FlyBasei | FBgn0003964, usp |
VEuPathDBi | VectorBase:FBgn0003964 |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000170897 |
HOGENOMi | CLU_007368_5_0_1 |
InParanoidi | P20153 |
OMAi | DEHCRQE |
OrthoDBi | 912470at2759 |
PhylomeDBi | P20153 |
Enzyme and pathway databases
Reactomei | R-DME-159418, Recycling of bile acids and salts R-DME-200425, Carnitine metabolism R-DME-204174, Regulation of pyruvate dehydrogenase (PDH) complex R-DME-381340, Transcriptional regulation of white adipocyte differentiation R-DME-383280, Nuclear Receptor transcription pathway R-DME-400206, Regulation of lipid metabolism by PPARalpha R-DME-5362517, Signaling by Retinoic Acid R-DME-9029569, NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux R-DME-9616222, Transcriptional regulation of granulopoiesis R-DME-9623433, NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis R-DME-9707564, Cytoprotection by HMOX1 |
SignaLinki | P20153 |
Miscellaneous databases
BioGRID-ORCSi | 31165, 0 hits in 3 CRISPR screens |
EvolutionaryTracei | P20153 |
GenomeRNAii | 31165 |
PROi | PR:P20153 |
Gene expression databases
Bgeei | FBgn0003964, Expressed in cleaving embryo and 42 other tissues |
ExpressionAtlasi | P20153, baseline and differential |
Genevisiblei | P20153, DM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
IDEALi | IID50250 |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR000003, Retinoid-X_rcpt/HNF4 IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR00545, RETINOIDXR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | USP_DROME | |
Accessioni | P20153Primary (citable) accession number: P20153 Secondary accession number(s): Q9W535 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | May 25, 2022 | |
This is version 228 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families