UniProtKB - P20112 (SPRC_PIG)
Protein
SPARC
Gene
SPARC
Organism
Sus scrofa (Pig)
Status
Functioni
Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca2+ with a low affinity and an EF-hand loop that binds a Ca2+ ion with a high affinity (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 271 – 282 | By similarityAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: GO_Central
- collagen binding Source: GO_Central
- extracellular matrix binding Source: GO_Central
GO - Biological processi
- anatomical structure development Source: GO_Central
Keywordsi
Ligand | Calcium, Copper, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: SPARCAlternative name(s): Basement-membrane protein 40 Short name: BM-40 Osteonectin Short name: ON Secreted protein acidic and rich in cysteine |
Gene namesi | Name:SPARC |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
Note: In or around the basement membrane.By similarity
Extracellular region or secreted
- basement membrane Source: UniProtKB-SubCell
- extracellular space Source: GO_Central
Keywords - Cellular componenti
Basement membrane, Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | 2 PublicationsAdd BLAST | 17 | |
ChainiPRO_0000160590 | 18 – 300 | SPARCAdd BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 69 ↔ 80 | PROSITE-ProRule annotation | ||
Disulfide bondi | 74 ↔ 90 | PROSITE-ProRule annotation | ||
Disulfide bondi | 92 ↔ 127 | PROSITE-ProRule annotation | ||
Disulfide bondi | 98 ↔ 120 | PROSITE-ProRule annotation | ||
Disulfide bondi | 109 ↔ 146 | PROSITE-ProRule annotation | ||
Glycosylationi | 113 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Disulfide bondi | 152 ↔ 262 | PROSITE-ProRule annotation | ||
Disulfide bondi | 270 ↔ 286 | PROSITE-ProRule annotation |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | P20112 |
PeptideAtlasi | P20112 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 68 – 90 | Follistatin-likeAdd BLAST | 23 | |
Domaini | 86 – 148 | Kazal-likePROSITE-ProRule annotationAdd BLAST | 63 | |
Domaini | 258 – 293 | EF-handAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 22 – 66 | Asp/Glu-rich (acidic; binds calcium)Add BLAST | 45 |
Sequence similaritiesi
Belongs to the SPARC family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4004, Eukaryota |
InParanoidi | P20112 |
OrthoDBi | 1528521at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P20112-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRAWIFFLLC LAGKALAAPQ QEALPDETEV VEETVAEVPV GANPVQVEVG
60 70 80 90 100
EFDDGAEEAE EEVVAENPCQ NHHCKHGKVC ELDENNSPMC VCQDPTSCPA
110 120 130 140 150
PIGEFEKVCS NDNKTFDSSC HFFATKCTLE GTKKGHKLHL DYIGPCKYIP
160 170 180 190 200
PCLDSELTEF PLRMRDWLKN VLVTLYERDE NNNLLTEKQK LRVKKIHENE
210 220 230 240 250
KRLEAGDHPV ELLARDFEKN YNMYIFPVHW QFGQLDQHPI DGYLSHTELA
260 270 280 290 300
PLRAPLIPME HCTTRFFQTC DLDNDKYIAL DEWAGCFGIK EQDIDKDLVI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY963262 mRNA Translation: AAX83050.1 |
PIRi | S00998 |
RefSeqi | NP_001026964.1, NM_001031794.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY963262 mRNA Translation: AAX83050.1 |
PIRi | S00998 |
RefSeqi | NP_001026964.1, NM_001031794.1 |
3D structure databases
SMRi | P20112 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9823.ENSSSCP00000018098 |
Proteomic databases
PaxDbi | P20112 |
PeptideAtlasi | P20112 |
Genome annotation databases
Organism-specific databases
CTDi | 6678 |
Phylogenomic databases
eggNOGi | KOG4004, Eukaryota |
InParanoidi | P20112 |
OrthoDBi | 1528521at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR003645, Fol_N IPR015369, Follistatin/Osteonectin_EGF IPR002350, Kazal_dom IPR036058, Kazal_dom_sf IPR001999, Osteonectin_CS IPR037641, SPARC IPR019577, SPARC/Testican_Ca-bd-dom |
PANTHERi | PTHR13866:SF6, PTHR13866:SF6, 1 hit |
Pfami | View protein in Pfam PF09289, FOLN, 1 hit PF00050, Kazal_1, 1 hit PF10591, SPARC_Ca_bdg, 1 hit |
SMARTi | View protein in SMART SM00274, FOLN, 1 hit SM00280, KAZAL, 1 hit |
SUPFAMi | SSF100895, SSF100895, 1 hit SSF47473, SSF47473, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS51465, KAZAL_2, 1 hit PS00612, OSTEONECTIN_1, 1 hit PS00613, OSTEONECTIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SPRC_PIG | |
Accessioni | P20112Primary (citable) accession number: P20112 Secondary accession number(s): Q4Z8Q2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | July 10, 2007 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families