The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - P20099 (BISC_ECOLI)
Protein
Biotin sulfoxide reductase
Gene
bisC
Organism
Escherichia coli (strain K12)
Status
Functioni
This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source. It reduces a spontaneous oxidation product of biotin, D-biotin D-sulfoxide (BSO or BDS), back to biotin. Also exhibits methionine-(S)-sulfoxide (Met-S-SO) reductase activity, acting specifically on the (S) enantiomer in the free, but not the protein-bound form. It thus plays a role in assimilation of oxidized methionines.
2 PublicationsMiscellaneous
Requires a small thioredoxin-like protein for activity.
Catalytic activityi
- [thioredoxin]-disulfide + H2O + L-methionine = [thioredoxin]-dithiol + L-methionine (S)-S-oxide1 PublicationEC:1.8.4.131 Publication
Cofactori
Mo-bis(molybdopterin guanine dinucleotide)2 PublicationsNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.2 Publications
Kineticsi
kcat is 3.2 min(-1) for the reduction of Met-S-SO using benzyl viologen as an artificial electron donor.
- KM=17 µM for L-methionine (S)-S-oxide1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 148 | Molybdenum (Mo-bis(molybdopterin guanine dinucleotide) metal ligand)By similarity | 1 |
GO - Molecular functioni
- electron transfer activity Source: GO_Central
- L-methionine:thioredoxin-disulfide S-oxidoreductase activity Source: EcoCyc
- molybdenum ion binding Source: EcoCyc
- molybdopterin cofactor binding Source: InterPro
GO - Biological processi
- anaerobic respiration Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, Molybdenum |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10124-MONOMER |
SABIO-RKi | P20099 |
Protein family/group databases
TCDBi | 5.A.3.4.3, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:bisC Ordered Locus Names:b3551, JW5940 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene together with metB1, msrA and msrB are unable to use Met-S-SO for growth but retain the ability to use the enantiomer Met-R-SO, while a metB1/msrA/msrB deletion mutant is able to use both compounds.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000063219 | 1 – 777 | Biotin sulfoxide reductaseAdd BLAST | 777 |
Proteomic databases
jPOSTi | P20099 |
PaxDbi | P20099 |
PRIDEi | P20099 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261247, 8 interactors |
STRINGi | 511145.b3551 |
Family & Domainsi
Domaini
The N-terminal 39 residues are essential for activity.1 Publication
Sequence similaritiesi
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.Curated
Phylogenomic databases
eggNOGi | COG0243, Bacteria |
HOGENOMi | CLU_000422_13_3_6 |
InParanoidi | P20099 |
OMAi | LEPDEWH |
PhylomeDBi | P20099 |
Family and domain databases
CDDi | cd02793, MopB_CT_DMSOR-BSOR-TMAOR, 1 hit |
InterProi | View protein in InterPro IPR009010, Asp_de-COase-like_dom_sf IPR006658, BisC IPR041954, CT_DMSOR/BSOR/TMAOR IPR041460, Molybdopterin_N IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006655, Mopterin_OxRdtase_prok_CS |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF18364, Molybdopterin_N, 1 hit PF01568, Molydop_binding, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR00509, bisC_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00490, MOLYBDOPTERIN_PROK_2, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit |
i Sequence
Sequence statusi: Complete.
P20099-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANSSSRYSV LTAAHWGPML VETDGETVFS SRGALATGME NSLQSAVRDQ
60 70 80 90 100
VHSNTRVRFP MVRKGFLASP ENPQGIRGQD EFVRVSWDEA LDLIHQQHKR
110 120 130 140 150
IREAYGPASI FAGSYGWRSN GVLHKASTLL QRYMALAGGY TGHLGDYSTG
160 170 180 190 200
AAQAIMPYVV GGSEVYQQQT SWPLVLEHSD VVVLWSANPL NTLKIAWNAS
210 220 230 240 250
DEQGLSYFSA LRDSGKKLIC IDPMRSETVD FFGDKMEWVA PHMGTDVALM
260 270 280 290 300
LGIAHTLVEN GWHDEAFLAR CTTGYAVFAS YLLGESDGIA KTAEWAAEIC
310 320 330 340 350
GVGAAKIREL AAIFHQNTTM LMAGWGMQRQ QFGEQKHWMI VTLAAMLGQI
360 370 380 390 400
GTPGGGFGLS YHFANGGNPT RRSAVLSSMQ GSLPGGCDAV DKIPVARIVE
410 420 430 440 450
ALENPGGAYQ HNGMNRHFPD IRFIWWAGGA NFTHHQDTNR LIRAWQKPEL
460 470 480 490 500
VVISECFWTA AAKHADIVLP ATTSFERNDL TMTGDYSNQH LVPMKQVVPP
510 520 530 540 550
RYEARNDFDV FAELSERWEK GGYARFTEGK SELQWLETFY NVARQRGASQ
560 570 580 590 600
QVELPPFAEF WQANQLIEMP ENPDSERFIR FADFCRDPLA HPLKTASGKI
610 620 630 640 650
EIFSQRIADY GYPDCPGHPM WLEPDEWQGN AEPEQLQVLS AHPAHRLHSQ
660 670 680 690 700
LNYSSLRELY AVANREPVTI HPDDAQERGI QDGDTVRLWN ARGQILAGAV
710 720 730 740 750
ISEGIKPGVI CIHEGAWPDL DLTADGICKN GAVNVLTKDL PSSRLGNGCA
760 770
GNTALAWLEK YNGPELTLTA FEPPASS
Sequence cautioni
The sequence AAB18528 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE77744 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 583 – 591 | DFCRDPLAH → AFLPRSAGD in AAA23522 (PubMed:2180922).Curated | 9 | |
Sequence conflicti | 596 – 598 | ASG → QR in AAA23522 (PubMed:2180922).Curated | 3 | |
Sequence conflicti | 747 – 777 | NGCAG…PPASS → MAVRVIRRWHGWKNTTVRN in AAA23522 (PubMed:2180922).CuratedAdd BLAST | 31 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34827 Genomic DNA Translation: AAA23522.1 Frameshift. U00039 Genomic DNA Translation: AAB18528.1 Different initiation. U00096 Genomic DNA Translation: AAC76575.3 AP009048 Genomic DNA Translation: BAE77744.1 Different initiation. |
PIRi | S47772 |
RefSeqi | NP_418007.3, NC_000913.3 WP_000013950.1, NZ_SSZK01000068.1 |
Genome annotation databases
EnsemblBacteriai | AAC76575; AAC76575; b3551 BAE77744; BAE77744; BAE77744 |
GeneIDi | 946915 |
KEGGi | ecj:JW5940 eco:b3551 |
PATRICi | fig|1411691.4.peg.3163 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M34827 Genomic DNA Translation: AAA23522.1 Frameshift. U00039 Genomic DNA Translation: AAB18528.1 Different initiation. U00096 Genomic DNA Translation: AAC76575.3 AP009048 Genomic DNA Translation: BAE77744.1 Different initiation. |
PIRi | S47772 |
RefSeqi | NP_418007.3, NC_000913.3 WP_000013950.1, NZ_SSZK01000068.1 |
3D structure databases
AlphaFoldDBi | P20099 |
SMRi | P20099 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261247, 8 interactors |
STRINGi | 511145.b3551 |
Protein family/group databases
TCDBi | 5.A.3.4.3, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Proteomic databases
jPOSTi | P20099 |
PaxDbi | P20099 |
PRIDEi | P20099 |
Genome annotation databases
EnsemblBacteriai | AAC76575; AAC76575; b3551 BAE77744; BAE77744; BAE77744 |
GeneIDi | 946915 |
KEGGi | ecj:JW5940 eco:b3551 |
PATRICi | fig|1411691.4.peg.3163 |
Organism-specific databases
EchoBASEi | EB0122 |
Phylogenomic databases
eggNOGi | COG0243, Bacteria |
HOGENOMi | CLU_000422_13_3_6 |
InParanoidi | P20099 |
OMAi | LEPDEWH |
PhylomeDBi | P20099 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10124-MONOMER |
SABIO-RKi | P20099 |
Miscellaneous databases
PROi | PR:P20099 |
Family and domain databases
CDDi | cd02793, MopB_CT_DMSOR-BSOR-TMAOR, 1 hit |
InterProi | View protein in InterPro IPR009010, Asp_de-COase-like_dom_sf IPR006658, BisC IPR041954, CT_DMSOR/BSOR/TMAOR IPR041460, Molybdopterin_N IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006655, Mopterin_OxRdtase_prok_CS |
Pfami | View protein in Pfam PF00384, Molybdopterin, 1 hit PF18364, Molybdopterin_N, 1 hit PF01568, Molydop_binding, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR00509, bisC_fam, 1 hit |
PROSITEi | View protein in PROSITE PS00490, MOLYBDOPTERIN_PROK_2, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BISC_ECOLI | |
Accessioni | P20099Primary (citable) accession number: P20099 Secondary accession number(s): Q2M7L2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | May 30, 2000 | |
Last modified: | May 25, 2022 | |
This is version 165 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families