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Entry version 165 (25 May 2022)
Sequence version 3 (30 May 2000)
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Protein

Biotin sulfoxide reductase

Gene

bisC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source. It reduces a spontaneous oxidation product of biotin, D-biotin D-sulfoxide (BSO or BDS), back to biotin. Also exhibits methionine-(S)-sulfoxide (Met-S-SO) reductase activity, acting specifically on the (S) enantiomer in the free, but not the protein-bound form. It thus plays a role in assimilation of oxidized methionines.

2 Publications

Miscellaneous

Requires a small thioredoxin-like protein for activity.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mo-bis(molybdopterin guanine dinucleotide)2 PublicationsNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.2 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 3.2 min(-1) for the reduction of Met-S-SO using benzyl viologen as an artificial electron donor.
  1. KM=17 µM for L-methionine (S)-S-oxide1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi148Molybdenum (Mo-bis(molybdopterin guanine dinucleotide) metal ligand)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • electron transfer activity Source: GO_Central
  • L-methionine:thioredoxin-disulfide S-oxidoreductase activity Source: EcoCyc
  • molybdenum ion binding Source: EcoCyc
  • molybdopterin cofactor binding Source: InterPro

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, Molybdenum

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG10124-MONOMER

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
P20099

Protein family/group databases

Transport Classification Database

More...
TCDBi
5.A.3.4.3, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Biotin sulfoxide reductase (EC:1.-.-.-)
Short name:
BDS reductase
Short name:
BSO reductase
Alternative name(s):
L-methionine-(S)-sulfoxide reductase (EC:1.8.4.13)
Short name:
Met-S-SO reductase
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:bisC
Ordered Locus Names:b3551, JW5940
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Cells lacking this gene together with metB1, msrA and msrB are unable to use Met-S-SO for growth but retain the ability to use the enantiomer Met-R-SO, while a metB1/msrA/msrB deletion mutant is able to use both compounds.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000632191 – 777Biotin sulfoxide reductaseAdd BLAST777

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P20099

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P20099

PRoteomics IDEntifications database

More...
PRIDEi
P20099

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4261247, 8 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b3551

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

AlphaFold Protein Structure Database

More...
AlphaFoldDBi
P20099

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P20099

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The N-terminal 39 residues are essential for activity.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG0243, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_000422_13_3_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P20099

Identification of Orthologs from Complete Genome Data

More...
OMAi
LEPDEWH

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P20099

Family and domain databases

Conserved Domains Database

More...
CDDi
cd02793, MopB_CT_DMSOR-BSOR-TMAOR, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR009010, Asp_de-COase-like_dom_sf
IPR006658, BisC
IPR041954, CT_DMSOR/BSOR/TMAOR
IPR041460, Molybdopterin_N
IPR006657, MoPterin_dinucl-bd_dom
IPR006656, Mopterin_OxRdtase
IPR006655, Mopterin_OxRdtase_prok_CS

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00384, Molybdopterin, 1 hit
PF18364, Molybdopterin_N, 1 hit
PF01568, Molydop_binding, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50692, SSF50692, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00509, bisC_fam, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00490, MOLYBDOPTERIN_PROK_2, 1 hit
PS00932, MOLYBDOPTERIN_PROK_3, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P20099-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MANSSSRYSV LTAAHWGPML VETDGETVFS SRGALATGME NSLQSAVRDQ
60 70 80 90 100
VHSNTRVRFP MVRKGFLASP ENPQGIRGQD EFVRVSWDEA LDLIHQQHKR
110 120 130 140 150
IREAYGPASI FAGSYGWRSN GVLHKASTLL QRYMALAGGY TGHLGDYSTG
160 170 180 190 200
AAQAIMPYVV GGSEVYQQQT SWPLVLEHSD VVVLWSANPL NTLKIAWNAS
210 220 230 240 250
DEQGLSYFSA LRDSGKKLIC IDPMRSETVD FFGDKMEWVA PHMGTDVALM
260 270 280 290 300
LGIAHTLVEN GWHDEAFLAR CTTGYAVFAS YLLGESDGIA KTAEWAAEIC
310 320 330 340 350
GVGAAKIREL AAIFHQNTTM LMAGWGMQRQ QFGEQKHWMI VTLAAMLGQI
360 370 380 390 400
GTPGGGFGLS YHFANGGNPT RRSAVLSSMQ GSLPGGCDAV DKIPVARIVE
410 420 430 440 450
ALENPGGAYQ HNGMNRHFPD IRFIWWAGGA NFTHHQDTNR LIRAWQKPEL
460 470 480 490 500
VVISECFWTA AAKHADIVLP ATTSFERNDL TMTGDYSNQH LVPMKQVVPP
510 520 530 540 550
RYEARNDFDV FAELSERWEK GGYARFTEGK SELQWLETFY NVARQRGASQ
560 570 580 590 600
QVELPPFAEF WQANQLIEMP ENPDSERFIR FADFCRDPLA HPLKTASGKI
610 620 630 640 650
EIFSQRIADY GYPDCPGHPM WLEPDEWQGN AEPEQLQVLS AHPAHRLHSQ
660 670 680 690 700
LNYSSLRELY AVANREPVTI HPDDAQERGI QDGDTVRLWN ARGQILAGAV
710 720 730 740 750
ISEGIKPGVI CIHEGAWPDL DLTADGICKN GAVNVLTKDL PSSRLGNGCA
760 770
GNTALAWLEK YNGPELTLTA FEPPASS
Length:777
Mass (Da):85,851
Last modified:May 30, 2000 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i51087D957E4FB38B
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAB18528 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE77744 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti583 – 591DFCRDPLAH → AFLPRSAGD in AAA23522 (PubMed:2180922).Curated9
Sequence conflicti596 – 598ASG → QR in AAA23522 (PubMed:2180922).Curated3
Sequence conflicti747 – 777NGCAG…PPASS → MAVRVIRRWHGWKNTTVRN in AAA23522 (PubMed:2180922).CuratedAdd BLAST31

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
M34827 Genomic DNA Translation: AAA23522.1 Frameshift.
U00039 Genomic DNA Translation: AAB18528.1 Different initiation.
U00096 Genomic DNA Translation: AAC76575.3
AP009048 Genomic DNA Translation: BAE77744.1 Different initiation.

Protein sequence database of the Protein Information Resource

More...
PIRi
S47772

NCBI Reference Sequences

More...
RefSeqi
NP_418007.3, NC_000913.3
WP_000013950.1, NZ_SSZK01000068.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC76575; AAC76575; b3551
BAE77744; BAE77744; BAE77744

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
946915

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW5940
eco:b3551

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.3163

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M34827 Genomic DNA Translation: AAA23522.1 Frameshift.
U00039 Genomic DNA Translation: AAB18528.1 Different initiation.
U00096 Genomic DNA Translation: AAC76575.3
AP009048 Genomic DNA Translation: BAE77744.1 Different initiation.
PIRiS47772
RefSeqiNP_418007.3, NC_000913.3
WP_000013950.1, NZ_SSZK01000068.1

3D structure databases

AlphaFoldDBiP20099
SMRiP20099
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4261247, 8 interactors
STRINGi511145.b3551

Protein family/group databases

TCDBi5.A.3.4.3, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

Proteomic databases

jPOSTiP20099
PaxDbiP20099
PRIDEiP20099

Genome annotation databases

EnsemblBacteriaiAAC76575; AAC76575; b3551
BAE77744; BAE77744; BAE77744
GeneIDi946915
KEGGiecj:JW5940
eco:b3551
PATRICifig|1411691.4.peg.3163

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0122

Phylogenomic databases

eggNOGiCOG0243, Bacteria
HOGENOMiCLU_000422_13_3_6
InParanoidiP20099
OMAiLEPDEWH
PhylomeDBiP20099

Enzyme and pathway databases

BioCyciEcoCyc:EG10124-MONOMER
SABIO-RKiP20099

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P20099

Family and domain databases

CDDicd02793, MopB_CT_DMSOR-BSOR-TMAOR, 1 hit
InterProiView protein in InterPro
IPR009010, Asp_de-COase-like_dom_sf
IPR006658, BisC
IPR041954, CT_DMSOR/BSOR/TMAOR
IPR041460, Molybdopterin_N
IPR006657, MoPterin_dinucl-bd_dom
IPR006656, Mopterin_OxRdtase
IPR006655, Mopterin_OxRdtase_prok_CS
PfamiView protein in Pfam
PF00384, Molybdopterin, 1 hit
PF18364, Molybdopterin_N, 1 hit
PF01568, Molydop_binding, 1 hit
SUPFAMiSSF50692, SSF50692, 1 hit
TIGRFAMsiTIGR00509, bisC_fam, 1 hit
PROSITEiView protein in PROSITE
PS00490, MOLYBDOPTERIN_PROK_2, 1 hit
PS00932, MOLYBDOPTERIN_PROK_3, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBISC_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P20099
Secondary accession number(s): Q2M7L2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: May 30, 2000
Last modified: May 25, 2022
This is version 165 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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