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UniProtKB - P20083 (PARE_ECOLI)
Protein
DNA topoisomerase 4 subunit B
Gene
parE
Organism
Escherichia coli (strain K12)
Status
Functioni
Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes (PubMed:9334322).
It relaxes supercoiled DNA (PubMed:15105144, PubMed:21300644, PubMed:23294697, PubMed:23352267).
MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation (PubMed:20921377).
6 PublicationsCatalytic activityi
- ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation2 Publications EC:5.6.2.2
Cofactori
Mg2+UniRule annotation1 Publication, Mn2+UniRule annotation1 Publication, Ca2+UniRule annotation1 PublicationNote: Binds two Mg2+ per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn2+ or Ca2+.UniRule annotation1 Publication
Activity regulationi
Pyrrolopyrimidines inhibit both GyrB and its paralog in topoisomerase IV (parE) (PubMed:23294697).1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 5 | ATP1 Publication | 1 | |
Binding sitei | 42 | ATP1 Publication | 1 | |
Binding sitei | 69 | ATP1 Publication | 1 | |
Binding sitei | 334 | ATP1 Publication | 1 | |
Metal bindingi | 418 | Magnesium 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 490 | Magnesium 1; catalyticUniRule annotation | 1 | |
Metal bindingi | 490 | Magnesium 2UniRule annotation | 1 | |
Metal bindingi | 492 | Magnesium 2UniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 110 – 116 | ATP1 Publication | 7 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- DNA binding Source: UniProtKB-UniRule
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- chromosome organization Source: EcoliWiki
- DNA topological change Source: GO_Central
- plasmid partitioning Source: EcoliWiki
- response to antibiotic Source: UniProtKB-KW
- sister chromatid cohesion Source: EcoliWiki
Keywordsi
Molecular function | DNA-binding, Isomerase, Topoisomerase |
Biological process | Antibiotic resistance |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10687-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA topoisomerase 4 subunit BUniRule annotation (EC:5.6.2.2UniRule annotation2 Publications)Alternative name(s): Topoisomerase IV subunit BUniRule annotation |
Gene namesi | Name:parEUniRule annotation Synonyms:nfxD Ordered Locus Names:b3030, JW2998 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL3329081 |
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
DrugCentrali | P20083 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145427 | 1 – 630 | DNA topoisomerase 4 subunit BAdd BLAST | 630 |
Proteomic databases
jPOSTi | P20083 |
PaxDbi | P20083 |
PRIDEi | P20083 |
Interactioni
Subunit structurei
Heterotetramer composed of ParC and ParE.
UniRule annotation2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 443 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 446 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 497 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 615 | Interaction with DNAUniRule annotation | 1 |
Binary interactionsi
P20083
With | #Exp. | IntAct |
---|---|---|
dhaK [P76015] | 3 | EBI-547277,EBI-544485 |
Protein-protein interaction databases
BioGRIDi | 4262393, 305 interactors 851818, 2 interactors |
ComplexPortali | CPX-1104, Topoisomerase IV |
DIPi | DIP-10441N |
IntActi | P20083, 25 interactors |
STRINGi | 511145.b3030 |
Chemistry databases
BindingDBi | P20083 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P20083 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P20083 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 412 – 525 | ToprimUniRule annotationAdd BLAST | 114 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0187, Bacteria |
HOGENOMi | CLU_006146_4_1_6 |
InParanoidi | P20083 |
PhylomeDBi | P20083 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.565.10, 1 hit 3.40.50.670, 1 hit |
HAMAPi | MF_00938, ParE_type1, 1 hit |
InterProi | View protein in InterPro IPR002288, DNA_gyrase_B_C IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR001241, Topo_IIA IPR013760, Topo_IIA-like_dom_sf IPR013759, Topo_IIA_B_C IPR013506, Topo_IIA_bsu_dom2 IPR018522, TopoIIA_CS IPR005737, TopoIV_B_Gneg IPR006171, TOPRIM_domain |
Pfami | View protein in Pfam PF00204, DNA_gyraseB, 1 hit PF00986, DNA_gyraseB_C, 1 hit PF02518, HATPase_c, 1 hit PF01751, Toprim, 1 hit |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00433, TOP2c, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55874, SSF55874, 1 hit SSF56719, SSF56719, 1 hit |
TIGRFAMsi | TIGR01055, parE_Gneg, 1 hit |
PROSITEi | View protein in PROSITE PS00177, TOPOISOMERASE_II, 1 hit PS50880, TOPRIM, 1 hit |
i Sequence
Sequence statusi: Complete.
P20083-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQTYNADAI EVLTGLEPVR RRPGMYTDTT RPNHLGQEVI DNSVDEALAG
60 70 80 90 100
HAKRVDVILH ADQSLEVIDD GRGMPVDIHP EEGVPAVELI LCRLHAGGKF
110 120 130 140 150
SNKNYQFSGG LHGVGISVVN ALSKRVEVNV RRDGQVYNIA FENGEKVQDL
160 170 180 190 200
QVVGTCGKRN TGTSVHFWPD ETFFDSPRFS VSRLTHVLKA KAVLCPGVEI
210 220 230 240 250
TFKDEINNTE QRWCYQDGLN DYLAEAVNGL PTLPEKPFIG NFAGDTEAVD
260 270 280 290 300
WALLWLPEGG ELLTESYVNL IPTMQGGTHV NGLRQGLLDA MREFCEYRNI
310 320 330 340 350
LPRGVKLSAE DIWDRCAYVL SVKMQDPQFA GQTKERLSSR QCAAFVSGVV
360 370 380 390 400
KDAFILWLNQ NVQAAELLAE MAISSAQRRM RAAKKVVRKK LTSGPALPGK
410 420 430 440 450
LADCTAQDLN RTELFLVEGD SAGGSAKQAR DREYQAIMPL KGKILNTWEV
460 470 480 490 500
SSDEVLASQE VHDISVAIGI DPDSDDLSQL RYGKICILAD ADSDGLHIAT
510 520 530 540 550
LLCALFVKHF RALVKHGHVY VALPPLYRID LGKEVYYALT EEEKEGVLEQ
560 570 580 590 600
LKRKKGKPNV QRFKGLGEMN PMQLRETTLD PNTRRLVQLT IDDEDDQRTD
610 620 630
AMMDMLLAKK RSEDRRNWLQ EKGDMAEIEV
Sequence cautioni
The sequence AAA24298 differs from that shown. Reason: Frameshift.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 445 | L → H in strain: DH161; quinolone-resistant. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58409 Genomic DNA Translation: AAA24298.1 Frameshift. U28377 Genomic DNA Translation: AAA69198.1 U00096 Genomic DNA Translation: AAC76066.1 AP009048 Genomic DNA Translation: BAE77086.1 L22026 Unassigned DNA Translation: AAC36841.1 |
PIRi | D65090 |
RefSeqi | NP_417502.1, NC_000913.3 WP_000195296.1, NZ_SSUR01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC76066; AAC76066; b3030 BAE77086; BAE77086; BAE77086 |
GeneIDi | 66673071 947501 |
KEGGi | ecj:JW2998 eco:b3030 |
PATRICi | fig|1411691.4.peg.3701 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58409 Genomic DNA Translation: AAA24298.1 Frameshift. U28377 Genomic DNA Translation: AAA69198.1 U00096 Genomic DNA Translation: AAC76066.1 AP009048 Genomic DNA Translation: BAE77086.1 L22026 Unassigned DNA Translation: AAC36841.1 |
PIRi | D65090 |
RefSeqi | NP_417502.1, NC_000913.3 WP_000195296.1, NZ_SSUR01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1S14 | X-ray | 2.00 | A/B | 1-217 | [»] | |
1S16 | X-ray | 2.10 | A/B | 1-390 | [»] | |
3FV5 | X-ray | 1.80 | A/B | 15-215 | [»] | |
4HZ0 | X-ray | 2.20 | A/B | 12-216 | [»] | |
SMRi | P20083 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262393, 305 interactors 851818, 2 interactors |
ComplexPortali | CPX-1104, Topoisomerase IV |
DIPi | DIP-10441N |
IntActi | P20083, 25 interactors |
STRINGi | 511145.b3030 |
Chemistry databases
BindingDBi | P20083 |
ChEMBLi | CHEMBL3329081 |
DrugBanki | DB04395, Phosphoaminophosphonic Acid-Adenylate Ester |
DrugCentrali | P20083 |
Proteomic databases
jPOSTi | P20083 |
PaxDbi | P20083 |
PRIDEi | P20083 |
Genome annotation databases
EnsemblBacteriai | AAC76066; AAC76066; b3030 BAE77086; BAE77086; BAE77086 |
GeneIDi | 66673071 947501 |
KEGGi | ecj:JW2998 eco:b3030 |
PATRICi | fig|1411691.4.peg.3701 |
Organism-specific databases
EchoBASEi | EB0681 |
Phylogenomic databases
eggNOGi | COG0187, Bacteria |
HOGENOMi | CLU_006146_4_1_6 |
InParanoidi | P20083 |
PhylomeDBi | P20083 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10687-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P20083 |
PROi | PR:P20083 |
Family and domain databases
Gene3Di | 3.30.230.10, 1 hit 3.30.565.10, 1 hit 3.40.50.670, 1 hit |
HAMAPi | MF_00938, ParE_type1, 1 hit |
InterProi | View protein in InterPro IPR002288, DNA_gyrase_B_C IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR020568, Ribosomal_S5_D2-typ_fold IPR014721, Ribosomal_S5_D2-typ_fold_subgr IPR001241, Topo_IIA IPR013760, Topo_IIA-like_dom_sf IPR013759, Topo_IIA_B_C IPR013506, Topo_IIA_bsu_dom2 IPR018522, TopoIIA_CS IPR005737, TopoIV_B_Gneg IPR006171, TOPRIM_domain |
Pfami | View protein in Pfam PF00204, DNA_gyraseB, 1 hit PF00986, DNA_gyraseB_C, 1 hit PF02518, HATPase_c, 1 hit PF01751, Toprim, 1 hit |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00433, TOP2c, 1 hit |
SUPFAMi | SSF54211, SSF54211, 1 hit SSF55874, SSF55874, 1 hit SSF56719, SSF56719, 1 hit |
TIGRFAMsi | TIGR01055, parE_Gneg, 1 hit |
PROSITEi | View protein in PROSITE PS00177, TOPOISOMERASE_II, 1 hit PS50880, TOPRIM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PARE_ECOLI | |
Accessioni | P20083Primary (citable) accession number: P20083 Secondary accession number(s): Q2M9H0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1994 | |
Last modified: | February 23, 2022 | |
This is version 185 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families