UniProtKB - P20067 (ID1_MOUSE)
DNA-binding protein inhibitor ID-1
Id1
Functioni
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.
2 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- proteasome binding Source: MGI
- protein C-terminus binding Source: MGI
- protein dimerization activity Source: InterPro
- protein N-terminus binding Source: MGI
- protein self-association Source: MGI
- transcription regulator inhibitor activity Source: UniProtKB
GO - Biological processi
- BMP signaling pathway Source: MGI
- cell differentiation Source: GO_Central
- circadian regulation of gene expression Source: GO_Central
- circadian rhythm Source: UniProtKB
- collagen metabolic process Source: MGI
- endothelial cell morphogenesis Source: MGI
- heart development Source: MGI
- lung morphogenesis Source: MGI
- lung vasculature development Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- negative regulation of dendrite morphogenesis Source: MGI
- negative regulation of DNA binding Source: MGI
- negative regulation of DNA-binding transcription factor activity Source: MGI
- negative regulation of endothelial cell differentiation Source: MGI
- negative regulation of gene expression Source: MGI
- negative regulation of osteoblast differentiation Source: MGI
- negative regulation of protein binding Source: MGI
- negative regulation of transcription, DNA-templated Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: MGI
- positive regulation of actin filament bundle assembly Source: MGI
- positive regulation of epithelial cell proliferation Source: MGI
- positive regulation of gene expression Source: UniProtKB
- protein destabilization Source: MGI
- regulation of angiogenesis Source: MGI
- regulation of MAPK cascade Source: MGI
- regulation of vasculature development Source: MGI
- response to antibiotic Source: MGI
Keywordsi
Molecular function | Developmental protein, Repressor |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-2559585, Oncogene Induced Senescence |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-binding protein inhibitor ID-1Alternative name(s): Inhibitor of DNA binding 1 Inhibitor of differentiation 1 |
Gene namesi | Name:Id1 Synonyms:Id, Id-1, Idb1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:96396, Id1 |
VEuPathDBi | HostDB:ENSMUSG00000042745 |
Subcellular locationi
Cytoskeleton
- centrosome Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127237 | 1 – 168 | DNA-binding protein inhibitor ID-1Add BLAST | 168 |
Post-translational modificationi
Keywords - PTMi
Ubl conjugationProteomic databases
MaxQBi | P20067 |
PaxDbi | P20067 |
PRIDEi | P20067 |
ProteomicsDBi | 267189 [P20067-1] 267190 [P20067-2] |
PTM databases
iPTMneti | P20067 |
PhosphoSitePlusi | P20067 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSMUSG00000042745, Expressed in mucous cell of stomach and 368 other tissues |
ExpressionAtlasi | P20067, baseline and differential |
Genevisiblei | P20067, MM |
Interactioni
Subunit structurei
Heterodimer with other HLH proteins.
Interacts with CLOCK and ARNTL/BMAL1 (By similarity).
Interacts with COPS5, IFI204, GATA4 and NKX2-5.
By similarity3 PublicationsGO - Molecular functioni
- identical protein binding Source: MGI
- protein C-terminus binding Source: MGI
- protein dimerization activity Source: InterPro
- protein N-terminus binding Source: MGI
- protein self-association Source: MGI
Protein-protein interaction databases
IntActi | P20067, 3 interactors |
MINTi | P20067 |
STRINGi | 10090.ENSMUSP00000092019 |
Miscellaneous databases
RNActi | P20067, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P20067 |
SMRi | P20067 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 98 | bHLHPROSITE-ProRule annotationAdd BLAST | 53 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 53 – 106 | Interaction with IFI204By similarityAdd BLAST | 54 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 91 – 104 | Nuclear export signalAdd BLAST | 14 |
Phylogenomic databases
eggNOGi | ENOG502RZP5, Eukaryota |
GeneTreei | ENSGT00940000161109 |
HOGENOMi | CLU_116790_0_0_1 |
InParanoidi | P20067 |
OMAi | QMNVFLQ |
PhylomeDBi | P20067 |
TreeFami | TF326217 |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR026052, DNA-bd_prot-inh IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR11723, PTHR11723, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKVASGSAAA AAGPSCSLKA GRTAGEVVLG LSEQSVAISR CAGTRLPALL
60 70 80 90 100
DEQQVNVLLY DMNGCYSRLK ELVPTLPQNR KVSKVEILQH VIDYIRDLQL
110 120 130 140 150
ELNSESEVGT TGGRGLPVRA PLSTLNGEIS ALAAEVRSES EYYIILQWET
160
EATGGGCPPS LLFRRIAI
The sequence of this isoform differs from the canonical sequence as follows:
136-168: VRSESEYYIILQWETEATGGGCPPSLLFRRIAI → AACVPADDRILCR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2AHY3 | A2AHY3_MOUSE | DNA-binding protein inhibitor ID-1 | Id1 | 168 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 140 | S → A in BAB25564 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_002109 | 136 – 168 | VRSES…RRIAI → AACVPADDRILCR in isoform Short. 1 PublicationAdd BLAST | 33 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31885 mRNA Translation: AAA37879.1 Different initiation. U43884 mRNA Translation: AAC52760.1 AK008264 mRNA Translation: BAB25564.1 |
CCDSi | CCDS16897.1 [P20067-2] CCDS89567.1 [P20067-1] |
PIRi | A34690 S72171 |
Genome annotation databases
Ensembli | ENSMUST00000038368; ENSMUSP00000092019; ENSMUSG00000042745 [P20067-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M31885 mRNA Translation: AAA37879.1 Different initiation. U43884 mRNA Translation: AAC52760.1 AK008264 mRNA Translation: BAB25564.1 |
CCDSi | CCDS16897.1 [P20067-2] CCDS89567.1 [P20067-1] |
PIRi | A34690 S72171 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6MGM | X-ray | 1.79 | A/B | 52-104 | [»] | |
6MGN | X-ray | 1.90 | B | 58-104 | [»] | |
6U2U | X-ray | 1.50 | A/B | 59-104 | [»] | |
AlphaFoldDBi | P20067 | |||||
SMRi | P20067 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P20067, 3 interactors |
MINTi | P20067 |
STRINGi | 10090.ENSMUSP00000092019 |
PTM databases
iPTMneti | P20067 |
PhosphoSitePlusi | P20067 |
Proteomic databases
MaxQBi | P20067 |
PaxDbi | P20067 |
PRIDEi | P20067 |
ProteomicsDBi | 267189 [P20067-1] 267190 [P20067-2] |
Protocols and materials databases
Antibodypediai | 25205, 398 antibodies from 37 providers |
Genome annotation databases
Ensembli | ENSMUST00000038368; ENSMUSP00000092019; ENSMUSG00000042745 [P20067-2] |
Organism-specific databases
MGIi | MGI:96396, Id1 |
VEuPathDBi | HostDB:ENSMUSG00000042745 |
Phylogenomic databases
eggNOGi | ENOG502RZP5, Eukaryota |
GeneTreei | ENSGT00940000161109 |
HOGENOMi | CLU_116790_0_0_1 |
InParanoidi | P20067 |
OMAi | QMNVFLQ |
PhylomeDBi | P20067 |
TreeFami | TF326217 |
Enzyme and pathway databases
Reactomei | R-MMU-2559585, Oncogene Induced Senescence |
Miscellaneous databases
ChiTaRSi | Id1, mouse |
PROi | PR:P20067 |
RNActi | P20067, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000042745, Expressed in mucous cell of stomach and 368 other tissues |
ExpressionAtlasi | P20067, baseline and differential |
Genevisiblei | P20067, MM |
Family and domain databases
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR026052, DNA-bd_prot-inh IPR036638, HLH_DNA-bd_sf |
PANTHERi | PTHR11723, PTHR11723, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ID1_MOUSE | |
Accessioni | P20067Primary (citable) accession number: P20067 Secondary accession number(s): Q61101, Q9D897 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | November 1, 1997 | |
Last modified: | May 25, 2022 | |
This is version 181 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references