UniProtKB - P20054 (PYR1_DICDI)
Protein
Protein PYR1-3
Gene
pyr1-3
Organism
Dictyostelium discoideum (Slime mold)
Status
Functioni
This protein is a 'fusion' protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase and DHOase).
Miscellaneous
GATase (glutamine amidotransferase) and CPSase (carbamoyl phosphate synthase) form together the glutamine-dependent CPSase (GD-CPSase) (EC 6.3.5.5).
Catalytic activityi
- 2 ATP + H2O + hydrogencarbonate + L-glutamine = 2 ADP + carbamoyl phosphate + 2 H+ + L-glutamate + phosphateEC:6.3.5.5
- EC:2.1.3.2
- EC:3.5.2.3
Cofactori
Zn2+CuratedNote: Binds 1 zinc ion per subunit (for dihydroorotase activity).Curated
Activity regulationi
Allosterically regulated and controlled by phosphorylation. 5-phosphoribose 1-diphosphate is an activator while UMP is an inhibitor of the CPSase reaction.
: UMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1, 2 and 3 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- Protein PYR1-3 (pyr1-3)
- Protein PYR1-3 (pyr1-3)
- Protein PYR1-3 (pyr1-3)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 275 | For GATase activityBy similarity | 1 | |
Active sitei | 361 | For GATase activityBy similarity | 1 | |
Active sitei | 363 | For GATase activityBy similarity | 1 | |
Metal bindingi | 1479 | ZincSequence analysis | 1 | |
Metal bindingi | 1481 | ZincSequence analysis | 1 |
GO - Molecular functioni
- amino acid binding Source: InterPro
- aspartate carbamoyltransferase activity Source: dictyBase
- ATP binding Source: UniProtKB-KW
- carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: dictyBase
- dihydroorotase activity Source: dictyBase
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: dictyBase
- 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
- glutamine metabolic process Source: dictyBase
- nitrogen compound metabolic process Source: GO_Central
Keywordsi
Molecular function | Allosteric enzyme, Hydrolase, Ligase, Multifunctional enzyme, Transferase |
Biological process | Pyrimidine biosynthesis |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-DDI-500753, Pyrimidine biosynthesis |
UniPathwayi | UPA00070;UER00115 UPA00070;UER00116 UPA00070;UER00117 |
Protein family/group databases
MEROPSi | M38.972 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pyr1-3 ORF Names:DDB_G0276335 |
Organismi | Dictyostelium discoideum (Slime mold) |
Taxonomic identifieri | 44689 [NCBI] |
Taxonomic lineagei | Eukaryota › Amoebozoa › Evosea › Eumycetozoa › Dictyostelia › Dictyosteliales › Dictyosteliaceae › Dictyostelium |
Proteomesi |
|
Organism-specific databases
dictyBasei | DDB_G0276335, pyr1-3 |
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199504 | 1 – 2225 | Protein PYR1-3Add BLAST | 2225 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | P20054 |
PRIDEi | P20054 |
Expressioni
Developmental stagei
Seen during growth but not during development.
Interactioni
Subunit structurei
Homohexamer.
Protein-protein interaction databases
STRINGi | 44689.DDB0201646 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 196 – 388 | Glutamine amidotransferase type-1Add BLAST | 193 | |
Domaini | 530 – 722 | ATP-grasp 1Add BLAST | 193 | |
Domaini | 1069 – 1260 | ATP-grasp 2Add BLAST | 192 | |
Domaini | 1324 – 1470 | MGS-likePROSITE-ProRule annotationAdd BLAST | 147 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 40 – 390 | GATase (Glutamine amidotransferase)Add BLAST | 351 | |
Regioni | 391 – 405 | LinkerAdd BLAST | 15 | |
Regioni | 406 – 1461 | CPSase (Carbamoyl-phosphate synthase)Add BLAST | 1056 | |
Regioni | 406 – 948 | CPSase AAdd BLAST | 543 | |
Regioni | 949 – 1461 | CPSase BAdd BLAST | 513 | |
Regioni | 1463 – 1797 | DHOase (dihydroorotase)Add BLAST | 335 | |
Regioni | 1798 – 1916 | LinkerAdd BLAST | 119 | |
Regioni | 1917 – 2225 | ATCase (Aspartate transcarbamylase)Add BLAST | 309 |
Sequence similaritiesi
In the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0370, Eukaryota |
HOGENOMi | CLU_000513_2_1_1 |
InParanoidi | P20054 |
OMAi | ADKCYFL |
PhylomeDBi | P20054 |
Family and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit 3.40.50.1370, 2 hits 3.40.50.1380, 1 hit 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_00001, Asp_carb_tr, 1 hit MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR002082, Asp_carbamoyltransf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR002195, Dihydroorotase_CS IPR017926, GATASE IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit PF02142, MGS, 1 hit PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit SM01097, CPSase_sm_chain, 1 hit SM00851, MGS, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit SSF52335, SSF52335, 1 hit SSF52440, SSF52440, 2 hits SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00670, asp_carb_tr, 1 hit TIGR01369, CPSaseII_lrg, 1 hit TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00097, CARBAMOYLTRANSFERASE, 1 hit PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS00482, DIHYDROOROTASE_1, 1 hit PS00483, DIHYDROOROTASE_2, 1 hit PS51273, GATASE_TYPE_1, 1 hit PS51855, MGS, 1 hit |
i Sequence
Sequence statusi: Complete.
P20054-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDILNRKKGC LVLEDGTKLS GYSFGSERSV AGECVFSTGM VGYNESISDP
60 70 80 90 100
SYTGQILVFS FPLIGNYGVP SFRERDPESG LAVNFESDKA HVQAIICSEY
110 120 130 140 150
CDEYSHWAAE KSLSEWLKES NIPGLYGIDT RALITKIREK GSLKGKVIIG
160 170 180 190 200
DFDESKLEFE DINLRNLVAE VSTKEIKEYK AAENNKKTGE KRKNKKVIVL
210 220 230 240 250
DCGIKNNQIR CLLNRGVDLK VVPWDYDVVA NESINDYDGV FISNGPGDPS
260 270 280 290 300
LCGKAIENIR KVLALPVAKA VFGVCMGNQL LGLAAGAQTH KMAFGNRGLN
310 320 330 340 350
QPCVDQISGR CHITSQNHGF VIDSNSLPAG SGWKTYFINA NDASNEGIYH
360 370 380 390 400
ESKPWFSVQF HPEAMAGPTD TEYLFDNFVD NVCGEQQHKS PMNKSKIIDC
410 420 430 440 450
PKGINKVLIL GSGGLSIGQA GEFDYSGSQA IKALKEEGIK TILINPNIAT
460 470 480 490 500
VQTSPGLADK VYFLPVNASS VQKVIENENP DGILVTFGGQ TALNCGIELY
510 520 530 540 550
KSGILEKYNC KVLGTPIETI IATEDRGIFA EKLSEINERI APSMACNSLE
560 570 580 590 600
ESLIEAEKIG YPVIVRAAYC LGGLGSGFAD NKEQLTALVT EAMATSSQVL
610 620 630 640 650
VEKSLKGWKE IEYEVLRDSK DNCITVCNME NFDPLGIHTG ESIVVAPSQT
660 670 680 690 700
LSDREYQMLR ETAIKTVRHL GVIGECNIQY SLNPYSEEYC IIEVNARLSR
710 720 730 740 750
SSALASKATG YPLAFISAKV ALGYDLAALR NTITKKTTAC FEPSLDYLVV
760 770 780 790 800
KMPRWDLKKF TRVSNKISSS MKSVGEVMSI GRKFEEAIQK AIRMVMDGAV
810 820 830 840 850
EGFQAGVFPT SDEELEHPTN NRILVLASAF KDGYSIDRVH QLTKIDKWFL
860 870 880 890 900
TKLKAIIDLE NHLSTYKEPS QIPSEILKFS KQQGFSDKQI ARAVGTTELN
910 920 930 940 950
VRDYRKKMGI IPCTKHIDTV AAEFPAQNNY LYMTYNGETN DVNINEKSYI
960 970 980 990 1000
TLGSGSYRIG SSVEFDWCAV SCIRTLRSLG LKSIMINFNP ETVSTDYDEC
1010 1020 1030 1040 1050
DYLYFEELSL ERVLDIYERG GPNSNHGVIL SVGGQIPNNL AIPLSRCNVK
1060 1070 1080 1090 1100
VLGTHPDMID SAENRYKFSR LLDTIGIDQP LWKELTSVSD TKDFCESVGF
1110 1120 1130 1140 1150
PCLVRPSYVL SGAAMNVVHS SQDLETFLTE AAAVSRDHPV VISKFIQEAK
1160 1170 1180 1190 1200
EIEIDAVADN GRIVLFAISE HVENAGVHSG DATIVCPAQD LDDATILKVE
1210 1220 1230 1240 1250
ETARKIAEAL NVSGPFNIQF IAKNNEIKVI ECNLRCSRSF PFVSKTLNIN
1260 1270 1280 1290 1300
FIELATKIII KHQYDLPVVN PINYVGVKVP QFSFIRLKGA DPVLGVEMAS
1310 1320 1330 1340 1350
TGEVACFGNT REEAYVKGLI STGFKAPEKN VLLSIGSFKE KHEFLPSAHK
1360 1370 1380 1390 1400
LIKLGYTLFG TQGTADFYSE NGVPVTQLNW DEEDLGENVI QKKMTENTIH
1410 1420 1430 1440 1450
LFINLPSKNK YRRPSSFMSR GYSLRRVAID FQVPLITNIK CAKLFVDSLS
1460 1470 1480 1490 1500
YMKGPMPIEN VDWRTSNKII RLPGLVDVHV HLREPGATHK EDWDSGTATA
1510 1520 1530 1540 1550
LAGGFTMVGA MPNTNPAIMD DASFELCKSL AASKARCDYG IFIGATFTNT
1560 1570 1580 1590 1600
TTAGKFASDA MGMKMYLEET FAPLPLKDDI NVWRDHIMNW PGTTPICVHA
1610 1620 1630 1640 1650
DGRNLAAILL LGWMYDKHMH VCHVSHKEEI DIIRDAKKRG MKLSCEVSPH
1660 1670 1680 1690 1700
HLTLCDKDIP RIGAGQSEVR PKLGTEEDLN ALWDNIDYID MIATDHAPHT
1710 1720 1730 1740 1750
WEEKCSAKPP PGFPGLETSL PLMLTAVHNG RITIEDLVMK MHTNPIRIFN
1760 1770 1780 1790 1800
LPEQPDTYIE VDMEQEWTIP KKPLYSRCGW TPFEGLQVRG KVVKVVLRGQ
1810 1820 1830 1840 1850
IAFIDGKIIA QKGFGLNLRS KEYQVEKERL LNTTKPIYDK IPTVQSTKNQ
1860 1870 1880 1890 1900
TTNITSPSLI SDSPNKAINK IKSTSTSTTP NTQEQSTQHL PLVGSNLASA
1910 1920 1930 1940 1950
VLNKKEDTLQ TAFNISDNSL AGKHIFSVKQ FNRKQLHALF GIAHEMRILV
1960 1970 1980 1990 2000
KRSGGSDLLK GKVLATLFYE PSTRTQCSFT AAMQRLGGSV VTVDNVSSSV
2010 2020 2030 2040 2050
AKGESIADTI QTLESYCDAV CMRHPAVGSV ESAIQVAKKP IINAGDGVGE
2060 2070 2080 2090 2100
HPTQALLDVF TIREELGTVN GLTITVVGDL KHGRTVHSLV RLLANYQVKI
2110 2120 2130 2140 2150
NYVSPSSLSM PTEIIKELNE KGIEQKEYTN IESILPTTNV LYVTRVQKER
2160 2170 2180 2190 2200
FQSIEEYEKV KDSFIITPHT LTKASDNMIV MHPLPRINEI SPEVDSDPRA
2210 2220
AYFRQMENGL YVRMSLLALV FGAGV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1224 – 1231 | NNEIKVIE → TMKSKLSN in CAA32782 (PubMed:2917570).Curated | 8 | |
Sequence conflicti | 1402 | Missing in CAA39077 (PubMed:1627825).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000014 Genomic DNA Translation: EAL69248.1 X14633 Genomic DNA Translation: CAA32781.1 X14634 Genomic DNA Translation: CAA32782.1 X55433 Genomic DNA Translation: CAA39077.1 |
PIRi | S02800, QZDOP3 S23738 |
RefSeqi | XP_643196.1, XM_638104.1 |
Genome annotation databases
EnsemblProtistsi | EAL69248; EAL69248; DDB_G0276335 |
GeneIDi | 8620470 |
KEGGi | ddi:DDB_G0276335 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAFI02000014 Genomic DNA Translation: EAL69248.1 X14633 Genomic DNA Translation: CAA32781.1 X14634 Genomic DNA Translation: CAA32782.1 X55433 Genomic DNA Translation: CAA39077.1 |
PIRi | S02800, QZDOP3 S23738 |
RefSeqi | XP_643196.1, XM_638104.1 |
3D structure databases
SMRi | P20054 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 44689.DDB0201646 |
Protein family/group databases
MEROPSi | M38.972 |
Proteomic databases
PaxDbi | P20054 |
PRIDEi | P20054 |
Genome annotation databases
EnsemblProtistsi | EAL69248; EAL69248; DDB_G0276335 |
GeneIDi | 8620470 |
KEGGi | ddi:DDB_G0276335 |
Organism-specific databases
dictyBasei | DDB_G0276335, pyr1-3 |
Phylogenomic databases
eggNOGi | KOG0370, Eukaryota |
HOGENOMi | CLU_000513_2_1_1 |
InParanoidi | P20054 |
OMAi | ADKCYFL |
PhylomeDBi | P20054 |
Enzyme and pathway databases
UniPathwayi | UPA00070;UER00115 UPA00070;UER00116 UPA00070;UER00117 |
Reactomei | R-DDI-500753, Pyrimidine biosynthesis |
Miscellaneous databases
PROi | PR:P20054 |
Family and domain databases
CDDi | cd01744, GATase1_CPSase, 1 hit |
Gene3Di | 1.10.1030.10, 1 hit 3.30.1490.20, 1 hit 3.40.50.1370, 2 hits 3.40.50.1380, 1 hit 3.40.50.880, 1 hit 3.50.30.20, 1 hit |
HAMAPi | MF_00001, Asp_carb_tr, 1 hit MF_01209, CPSase_S_chain, 1 hit |
InterProi | View protein in InterPro IPR006680, Amidohydro-rel IPR006132, Asp/Orn_carbamoyltranf_P-bd IPR006130, Asp/Orn_carbamoylTrfase IPR036901, Asp/Orn_carbamoylTrfase_sf IPR002082, Asp_carbamoyltransf IPR006131, Asp_carbamoyltransf_Asp/Orn-bd IPR011761, ATP-grasp IPR013815, ATP_grasp_subdomain_1 IPR006275, CarbamoylP_synth_lsu IPR005480, CarbamoylP_synth_lsu_oligo IPR036897, CarbamoylP_synth_lsu_oligo_sf IPR006274, CarbamoylP_synth_ssu IPR002474, CarbamoylP_synth_ssu_N IPR036480, CarbP_synth_ssu_N_sf IPR005479, CbamoylP_synth_lsu-like_ATP-bd IPR005483, CbamoylP_synth_lsu_CPSase_dom IPR029062, Class_I_gatase-like IPR035686, CPSase_GATase1 IPR002195, Dihydroorotase_CS IPR017926, GATASE IPR011059, Metal-dep_hydrolase_composite IPR032466, Metal_Hydrolase IPR011607, MGS-like_dom IPR036914, MGS-like_dom_sf IPR016185, PreATP-grasp_dom_sf |
Pfami | View protein in Pfam PF01979, Amidohydro_1, 1 hit PF02786, CPSase_L_D2, 2 hits PF02787, CPSase_L_D3, 1 hit PF00988, CPSase_sm_chain, 1 hit PF00117, GATase, 1 hit PF02142, MGS, 1 hit PF00185, OTCace, 1 hit PF02729, OTCace_N, 1 hit |
PRINTSi | PR00100, AOTCASE PR00098, CPSASE |
SMARTi | View protein in SMART SM01096, CPSase_L_D3, 1 hit SM01097, CPSase_sm_chain, 1 hit SM00851, MGS, 1 hit |
SUPFAMi | SSF48108, SSF48108, 1 hit SSF51338, SSF51338, 1 hit SSF51556, SSF51556, 1 hit SSF52021, SSF52021, 1 hit SSF52317, SSF52317, 1 hit SSF52335, SSF52335, 1 hit SSF52440, SSF52440, 2 hits SSF53671, SSF53671, 1 hit |
TIGRFAMsi | TIGR00670, asp_carb_tr, 1 hit TIGR01369, CPSaseII_lrg, 1 hit TIGR01368, CPSaseIIsmall, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 2 hits PS00097, CARBAMOYLTRANSFERASE, 1 hit PS00866, CPSASE_1, 2 hits PS00867, CPSASE_2, 2 hits PS00482, DIHYDROOROTASE_1, 1 hit PS00483, DIHYDROOROTASE_2, 1 hit PS51273, GATASE_TYPE_1, 1 hit PS51855, MGS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PYR1_DICDI | |
Accessioni | P20054Primary (citable) accession number: P20054 Secondary accession number(s): Q551R5, Q86AD0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | December 4, 2007 | |
Last modified: | December 2, 2020 | |
This is version 181 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Dictyostelium discoideum
Dictyostelium discoideum: entries, gene names and cross-references to dictyBase - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families