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Protein

Platelet-derived growth factor subunit A

Gene

Pdgfa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.7 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Growth factor, Mitogen

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-derived growth factor subunit A
Short name:
PDGF subunit A
Alternative name(s):
PDGF-1
Platelet-derived growth factor A chain
Platelet-derived growth factor alpha polypeptide
Gene namesi
Name:Pdgfa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97527 Pdgfa

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Lethal, due to defects in cell proliferation and migration, leading to defects in the development of the embryonic lung, gastrointestinal tract, oligodendrocytes and Leydig cells.7 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Add BLAST20
PropeptideiPRO_000002335821 – 86Removed in mature formAdd BLAST66
ChainiPRO_000002335987 – 211Platelet-derived growth factor subunit AAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi96 ↔ 140By similarity
Disulfide bondi123InterchainBy similarity
Disulfide bondi129 ↔ 177By similarity
Disulfide bondi132InterchainBy similarity
Disulfide bondi133 ↔ 179By similarity
Glycosylationi134N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP20033
PeptideAtlasiP20033
PRIDEiP20033

PTM databases

PhosphoSitePlusiP20033

Expressioni

Tissue specificityi

Expression primarily localized in papillary regions with presumable expression in tubular cells comprising the loop of Henle. In the renal cortex, a widespread expression seen in the vascular smooth muscle cells and is barely detectable in interstitial cells.1 Publication

Gene expression databases

CleanExiMM_PDGFA

Interactioni

Subunit structurei

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFB; antiparallel disulfide-linked dimer. The PDGFA homodimer interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRA homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with CSPG4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-2893 Platelet-derived growth factor AA complex
CPX-2899 PDGF receptor alpha - PDGF-AA complex
CPX-2900 Platelet-derived growth factor AB complex
CPX-2901 PDGF receptor alpha - PDGF-AB complex
CPX-2903 PDGF receptor alpha-beta - PDGF-AB complex
CPX-2904 PDGF receptor beta - PDGF-AB complex
STRINGi10090.ENSMUSP00000075463

Structurei

3D structure databases

ProteinModelPortaliP20033
SMRiP20033
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni158 – 162Receptor binding siteSequence analysis5

Domaini

The long form contains a basic insert which acts as a cell retention signal.

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IEXJ Eukaryota
ENOG4111GMH LUCA
HOGENOMiHOG000286027
HOVERGENiHBG053546
InParanoidiP20033
KOiK04359
PhylomeDBiP20033

Family and domain databases

CDDicd00135 PDGF, 1 hit
Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR023581 PD_growth_factor_CS
IPR000072 PDGF/VEGF_dom
IPR006782 PDGF_N
PfamiView protein in Pfam
PF00341 PDGF, 1 hit
PF04692 PDGF_N, 1 hit
SMARTiView protein in SMART
SM00141 PDGF, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00249 PDGF_1, 1 hit
PS50278 PDGF_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform Long (identifier: P20033-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MRTWACLLLL GCGYLAHALA EEAEIPRELI ERLARSQIHS IRDLQRLLEI
60 70 80 90 100
DSVGAEDALE TSLRAHGSHA INHVPEKRPV PIRRKRSIEE AVPAVCKTRT
110 120 130 140 150
VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV
160 170 180 190 200
KVAKVEYVRK KPKLKEVQVR LEEHLECACA TSNLNPDHRE EETGRRRESG
210
KNRKRKRLKP T
Length:211
Mass (Da):24,102
Last modified:October 1, 1996 - v2
Checksum:iAC4345A10ECF4B39
GO
Isoform Short (identifier: P20033-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     194-196: GRR → DVR
     197-211: Missing.

Show »
Length:196
Mass (Da):22,267
Checksum:i3F219DA70E4007D4
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q99L56Q99L56_MOUSE
Platelet derived growth factor, alp...
Pdgfa mCG_23666
196Annotation score:
G5E891G5E891_MOUSE
Platelet derived growth factor, alp...
Pdgfa mCG_23666
211Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92V → I in AAA39903 (PubMed:2155144).Curated1
Sequence conflicti174H → D in AAB28740 (PubMed:1340209).Curated1
Sequence conflicti174H → D in AAB28741 (PubMed:1340209).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004604194 – 196GRR → DVR in isoform Short. 2 Publications3
Alternative sequenceiVSP_004605197 – 211Missing in isoform Short. 2 PublicationsAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66873
, S66868, S66869, S66870, S66871, S66872 Genomic DNA Translation: AAB28740.2
S66874
, S66868, S66869, S66870, S66871, S66872 Genomic DNA Translation: AAB28741.2
M29464 mRNA Translation: AAA39903.1
CCDSiCCDS19802.1 [P20033-2]
PIRiA37359
RefSeqiNP_032834.1, NM_008808.3
UniGeneiMm.2675

Genome annotation databases

GeneIDi18590
KEGGimmu:18590

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S66873
, S66868, S66869, S66870, S66871, S66872 Genomic DNA Translation: AAB28740.2
S66874
, S66868, S66869, S66870, S66871, S66872 Genomic DNA Translation: AAB28741.2
M29464 mRNA Translation: AAA39903.1
CCDSiCCDS19802.1 [P20033-2]
PIRiA37359
RefSeqiNP_032834.1, NM_008808.3
UniGeneiMm.2675

3D structure databases

ProteinModelPortaliP20033
SMRiP20033
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-2893 Platelet-derived growth factor AA complex
CPX-2899 PDGF receptor alpha - PDGF-AA complex
CPX-2900 Platelet-derived growth factor AB complex
CPX-2901 PDGF receptor alpha - PDGF-AB complex
CPX-2903 PDGF receptor alpha-beta - PDGF-AB complex
CPX-2904 PDGF receptor beta - PDGF-AB complex
STRINGi10090.ENSMUSP00000075463

PTM databases

PhosphoSitePlusiP20033

Proteomic databases

PaxDbiP20033
PeptideAtlasiP20033
PRIDEiP20033

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18590
KEGGimmu:18590

Organism-specific databases

CTDi5154
MGIiMGI:97527 Pdgfa

Phylogenomic databases

eggNOGiENOG410IEXJ Eukaryota
ENOG4111GMH LUCA
HOGENOMiHOG000286027
HOVERGENiHBG053546
InParanoidiP20033
KOiK04359
PhylomeDBiP20033

Miscellaneous databases

PROiPR:P20033
SOURCEiSearch...

Gene expression databases

CleanExiMM_PDGFA

Family and domain databases

CDDicd00135 PDGF, 1 hit
Gene3Di2.10.90.10, 1 hit
InterProiView protein in InterPro
IPR029034 Cystine-knot_cytokine
IPR023581 PD_growth_factor_CS
IPR000072 PDGF/VEGF_dom
IPR006782 PDGF_N
PfamiView protein in Pfam
PF00341 PDGF, 1 hit
PF04692 PDGF_N, 1 hit
SMARTiView protein in SMART
SM00141 PDGF, 1 hit
SUPFAMiSSF57501 SSF57501, 1 hit
PROSITEiView protein in PROSITE
PS00249 PDGF_1, 1 hit
PS50278 PDGF_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPDGFA_MOUSE
AccessioniPrimary (citable) accession number: P20033
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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