UniProtKB - P19961 (AMY2B_HUMAN)
Protein
Alpha-amylase 2B
Gene
AMY2B
Organism
Homo sapiens (Human)
Status
Functioni
Catalytic activityi
- Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity EC:3.2.1.1
Cofactori
Protein has several cofactor binding sites:- Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
- chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 115 | CalciumBy similarity | 1 | |
Metal bindingi | 173 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 182 | CalciumBy similarity | 1 | |
Binding sitei | 210 | ChlorideBy similarity | 1 | |
Active sitei | 212 | NucleophileBy similarity | 1 | |
Metal bindingi | 216 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 248 | Proton donorBy similarity | 1 | |
Binding sitei | 313 | ChlorideBy similarity | 1 | |
Sitei | 315 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 352 | ChlorideBy similarity | 1 |
GO - Molecular functioni
- alpha-amylase activity Source: UniProtKB
- alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- carbohydrate metabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism |
Ligand | Calcium, Chloride, Metal-binding |
Enzyme and pathway databases
PathwayCommonsi | P19961 |
Reactomei | R-HSA-189085, Digestion of dietary carbohydrate |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-amylase 2B (EC:3.2.1.1By similarity)Alternative name(s): 1,4-alpha-D-glucan glucanohydrolase 2B Carcinoid alpha-amylase |
Gene namesi | Name:AMY2B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000240038.6 |
HGNCi | HGNC:478, AMY2B |
MIMi | 104660, gene |
neXtProti | NX_P19961 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 280 |
OpenTargetsi | ENSG00000240038 |
PharmGKBi | PA24785 |
Miscellaneous databases
Pharosi | P19961, Tbio |
Chemistry databases
DrugBanki | DB01870, 1,4-dithio-alpha-D-glucopyranose DB02730, 4-Methylthio-Alpha-D-Mannose DB03092, 5-Hydroxymethyl-Chonduritol DB03277, alpha-maltotriose DB02379, Beta-D-Glucose DB03323, Maltose DB02218, N-[4-hydroxymethyl-cyclohexan-6-yl-1,2,3-triol]-4,6-dideoxy-4-aminoglucopyranoside DB04417, P-Nitrophenol DB03088, Pidolic acid |
Polymorphism and mutation databases
BioMutai | AMY2B |
DMDMi | 113789 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | Add BLAST | 15 | |
ChainiPRO_0000001402 | 16 – 511 | Alpha-amylase 2BAdd BLAST | 496 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 16 | Pyrrolidone carboxylic acidBy similarity | 1 | |
Disulfide bondi | 43 ↔ 101 | By similarity | ||
Disulfide bondi | 85 ↔ 130 | By similarity | ||
Disulfide bondi | 156 ↔ 175 | By similarity | ||
Disulfide bondi | 393 ↔ 399 | By similarity | ||
Disulfide bondi | 465 ↔ 477 | By similarity |
Keywords - PTMi
Disulfide bond, Pyrrolidone carboxylic acidProteomic databases
jPOSTi | P19961 |
MassIVEi | P19961 |
MaxQBi | P19961 |
PaxDbi | P19961 |
PeptideAtlasi | P19961 |
PRIDEi | P19961 |
ProteomicsDBi | 3680 53704 [P19961-1] |
TopDownProteomicsi | P19961-1 [P19961-1] |
PTM databases
iPTMneti | P19961 |
PhosphoSitePlusi | P19961 |
Expressioni
Gene expression databases
Bgeei | ENSG00000240038, Expressed in pancreas and 115 other tissues |
ExpressionAtlasi | P19961, baseline and differential |
Genevisiblei | P19961, HS |
Organism-specific databases
HPAi | ENSG00000240038, Tissue enriched (pancreas) |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 106777, 4 interactors |
IntActi | P19961, 3 interactors |
STRINGi | 9606.ENSP00000354610 |
Miscellaneous databases
RNActi | P19961, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyl hydrolase 13 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG2212, Eukaryota |
GeneTreei | ENSGT00940000154802 |
HOGENOMi | CLU_013336_2_1_1 |
InParanoidi | P19961 |
OMAi | QEVIYGA |
OrthoDBi | 665362at2759 |
PhylomeDBi | P19961 |
TreeFami | TF312850 |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR031319, A-amylase_C IPR006046, Alpha_amylase IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PRINTSi | PR00110, ALPHAAMYLASE |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit SM00632, Aamy_C, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P19961-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MKFFLLLFTI GFCWAQYSPN TQQGRTSIVH LFEWRWVDIA LECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMSGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD
160 170 180 190 200
FNDGKCKTGS GDIENYNDAT QVRDCRLVGL LDLALEKDYV RSKIAEYMNH
210 220 230 240 250
LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNSNWFPAG SKPFIYQEVI
260 270 280 290 300
DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG
310 320 330 340 350
FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYRWP RQFQNGNDVN DWVGPPNNNG VIKEVTINPD TTCGNDWVCE
410 420 430 440 450
HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT
460 470 480 490 500
FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED
510
PFIAIHAESK L
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J2Z5 | C9J2Z5_HUMAN | Alpha-amylase 2B | AMY2B | 96 | Annotation score: | ||
C9JWK7 | C9JWK7_HUMAN | Alpha-amylase 2B | AMY2B | 86 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056932 | 368 – 370 | DVN → EHG in isoform 2. 1 Publication | 3 | |
Alternative sequenceiVSP_056933 | 371 – 511 | Missing in isoform 2. 1 PublicationAdd BLAST | 141 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24895 mRNA Translation: AAA35525.1 D90097 Genomic DNA Translation: BAA14130.1 AK095605 mRNA Translation: BAG53093.1 AK127047 mRNA Translation: BAG54429.1 AC105272 Genomic DNA No translation available. CH471097 Genomic DNA Translation: EAW72902.1 CH471097 Genomic DNA Translation: EAW72903.1 CH471097 Genomic DNA Translation: EAW72904.1 BC011179 mRNA Translation: AAH11179.1 BC020861 mRNA Translation: AAH20861.1 X07057 Genomic DNA Translation: CAA30100.1 M18670 Genomic DNA Translation: AAA51725.1 |
CCDSi | CCDS782.1 [P19961-1] |
PIRi | JS0165, ALHU2B |
RefSeqi | NP_066188.1, NM_020978.4 [P19961-1] |
Genome annotation databases
Ensembli | ENST00000361355; ENSP00000354610; ENSG00000240038 [P19961-1] ENST00000477657; ENSP00000433347; ENSG00000240038 [P19961-2] ENST00000610648; ENSP00000481588; ENSG00000240038 [P19961-1] |
GeneIDi | 280 |
KEGGi | hsa:280 |
UCSCi | uc001duq.5, human [P19961-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24895 mRNA Translation: AAA35525.1 D90097 Genomic DNA Translation: BAA14130.1 AK095605 mRNA Translation: BAG53093.1 AK127047 mRNA Translation: BAG54429.1 AC105272 Genomic DNA No translation available. CH471097 Genomic DNA Translation: EAW72902.1 CH471097 Genomic DNA Translation: EAW72903.1 CH471097 Genomic DNA Translation: EAW72904.1 BC011179 mRNA Translation: AAH11179.1 BC020861 mRNA Translation: AAH20861.1 X07057 Genomic DNA Translation: CAA30100.1 M18670 Genomic DNA Translation: AAA51725.1 |
CCDSi | CCDS782.1 [P19961-1] |
PIRi | JS0165, ALHU2B |
RefSeqi | NP_066188.1, NM_020978.4 [P19961-1] |
3D structure databases
SMRi | P19961 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 106777, 4 interactors |
IntActi | P19961, 3 interactors |
STRINGi | 9606.ENSP00000354610 |
Chemistry databases
DrugBanki | DB01870, 1,4-dithio-alpha-D-glucopyranose DB02730, 4-Methylthio-Alpha-D-Mannose DB03092, 5-Hydroxymethyl-Chonduritol DB03277, alpha-maltotriose DB02379, Beta-D-Glucose DB03323, Maltose DB02218, N-[4-hydroxymethyl-cyclohexan-6-yl-1,2,3-triol]-4,6-dideoxy-4-aminoglucopyranoside DB04417, P-Nitrophenol DB03088, Pidolic acid |
Protein family/group databases
CAZyi | GH13, Glycoside Hydrolase Family 13 |
PTM databases
iPTMneti | P19961 |
PhosphoSitePlusi | P19961 |
Polymorphism and mutation databases
BioMutai | AMY2B |
DMDMi | 113789 |
Proteomic databases
jPOSTi | P19961 |
MassIVEi | P19961 |
MaxQBi | P19961 |
PaxDbi | P19961 |
PeptideAtlasi | P19961 |
PRIDEi | P19961 |
ProteomicsDBi | 3680 53704 [P19961-1] |
TopDownProteomicsi | P19961-1 [P19961-1] |
Protocols and materials databases
Antibodypediai | 34942, 202 antibodies |
Genome annotation databases
Ensembli | ENST00000361355; ENSP00000354610; ENSG00000240038 [P19961-1] ENST00000477657; ENSP00000433347; ENSG00000240038 [P19961-2] ENST00000610648; ENSP00000481588; ENSG00000240038 [P19961-1] |
GeneIDi | 280 |
KEGGi | hsa:280 |
UCSCi | uc001duq.5, human [P19961-1] |
Organism-specific databases
CTDi | 280 |
DisGeNETi | 280 |
EuPathDBi | HostDB:ENSG00000240038.6 |
GeneCardsi | AMY2B |
HGNCi | HGNC:478, AMY2B |
HPAi | ENSG00000240038, Tissue enriched (pancreas) |
MIMi | 104660, gene |
neXtProti | NX_P19961 |
OpenTargetsi | ENSG00000240038 |
PharmGKBi | PA24785 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2212, Eukaryota |
GeneTreei | ENSGT00940000154802 |
HOGENOMi | CLU_013336_2_1_1 |
InParanoidi | P19961 |
OMAi | QEVIYGA |
OrthoDBi | 665362at2759 |
PhylomeDBi | P19961 |
TreeFami | TF312850 |
Enzyme and pathway databases
PathwayCommonsi | P19961 |
Reactomei | R-HSA-189085, Digestion of dietary carbohydrate |
Miscellaneous databases
BioGRID-ORCSi | 280, 8 hits in 806 CRISPR screens |
ChiTaRSi | AMY2B, human |
GeneWikii | AMY2B |
GenomeRNAii | 280 |
Pharosi | P19961, Tbio |
PROi | PR:P19961 |
RNActi | P19961, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000240038, Expressed in pancreas and 115 other tissues |
ExpressionAtlasi | P19961, baseline and differential |
Genevisiblei | P19961, HS |
Family and domain databases
Gene3Di | 2.60.40.1180, 1 hit |
InterProi | View protein in InterPro IPR006048, A-amylase/branching_C IPR031319, A-amylase_C IPR006046, Alpha_amylase IPR006047, Glyco_hydro_13_cat_dom IPR013780, Glyco_hydro_b IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00128, Alpha-amylase, 1 hit PF02806, Alpha-amylase_C, 1 hit |
PRINTSi | PR00110, ALPHAAMYLASE |
SMARTi | View protein in SMART SM00642, Aamy, 1 hit SM00632, Aamy_C, 1 hit |
SUPFAMi | SSF51445, SSF51445, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMY2B_HUMAN | |
Accessioni | P19961Primary (citable) accession number: P19961 Secondary accession number(s): B3KTI1 Q9UBH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 193 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries