Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Alpha-amylase 2B

Gene

AMY2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi115CalciumBy similarity1
Metal bindingi173Calcium; via carbonyl oxygenBy similarity1
Metal bindingi182CalciumBy similarity1
Binding sitei210ChlorideBy similarity1
Active sitei212NucleophileBy similarity1
Metal bindingi216Calcium; via carbonyl oxygenBy similarity1
Active sitei248Proton donorBy similarity1
Binding sitei313ChlorideBy similarity1
Sitei315Transition state stabilizerBy similarity1
Binding sitei352ChlorideBy similarity1

GO - Molecular functioni

  • alpha-amylase activity Source: UniProtKB
  • alpha-amylase activity (releasing maltohexaose) Source: UniProtKB-EC
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-HSA-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 2B (EC:3.2.1.1By similarity)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase 2B
Carcinoid alpha-amylase
Gene namesi
Name:AMY2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000240038.6
HGNCiHGNC:478 AMY2B
MIMi104660 gene
neXtProtiNX_P19961

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi280
OpenTargetsiENSG00000240038
PharmGKBiPA24785

Chemistry databases

DrugBankiDB02730 4-Methylthio-Alpha-D-Mannose
DB03092 5-Hydroxymethyl-Chonduritol
DB03277 alpha-maltotriose
DB02379 Beta-D-Glucose
DB03323 Maltose
DB04417 P-Nitrophenol
DB03088 Pyroglutamic Acid

Polymorphism and mutation databases

DMDMi113789

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
ChainiPRO_000000140216 – 511Alpha-amylase 2BAdd BLAST496

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi43 ↔ 101By similarity
Disulfide bondi85 ↔ 130By similarity
Disulfide bondi156 ↔ 175By similarity
Disulfide bondi393 ↔ 399By similarity
Disulfide bondi465 ↔ 477By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

MaxQBiP19961
PaxDbiP19961
PeptideAtlasiP19961
PRIDEiP19961
ProteomicsDBi53704
TopDownProteomicsiP19961-1 [P19961-1]

PTM databases

iPTMnetiP19961
PhosphoSitePlusiP19961

Expressioni

Gene expression databases

BgeeiENSG00000240038 Expressed in 88 organ(s), highest expression level in body of pancreas
CleanExiHS_AMY2B
ExpressionAtlasiP19961 baseline and differential
GenevisibleiP19961 HS

Organism-specific databases

HPAiHPA045394
HPA045399
HPA046980

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi106777, 3 interactors
IntActiP19961, 2 interactors
STRINGi9606.ENSP00000354610

Structurei

3D structure databases

ProteinModelPortaliP19961
SMRiP19961
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP19961
KOiK01176
OMAiWKCQHAW
OrthoDBiEOG091G07V6
PhylomeDBiP19961
TreeFamiTF312850

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P19961-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKFFLLLFTI GFCWAQYSPN TQQGRTSIVH LFEWRWVDIA LECERYLAPK
60 70 80 90 100
GFGGVQVSPP NENVAIHNPF RPWWERYQPV SYKLCTRSGN EDEFRNMVTR
110 120 130 140 150
CNNVGVRIYV DAVINHMSGN AVSAGTSSTC GSYFNPGSRD FPAVPYSGWD
160 170 180 190 200
FNDGKCKTGS GDIENYNDAT QVRDCRLVGL LDLALEKDYV RSKIAEYMNH
210 220 230 240 250
LIDIGVAGFR LDASKHMWPG DIKAILDKLH NLNSNWFPAG SKPFIYQEVI
260 270 280 290 300
DLGGEPIKSS DYFGNGRVTE FKYGAKLGTV IRKWNGEKMS YLKNWGEGWG
310 320 330 340 350
FMPSDRALVF VDNHDNQRGH GAGGASILTF WDARLYKMAV GFMLAHPYGF
360 370 380 390 400
TRVMSSYRWP RQFQNGNDVN DWVGPPNNNG VIKEVTINPD TTCGNDWVCE
410 420 430 440 450
HRWRQIRNMV NFRNVVDGQP FTNWYDNGSN QVAFGRGNRG FIVFNNDDWT
460 470 480 490 500
FSLTLQTGLP AGTYCDVISG DKINGNCTGI KIYVSDDGKA HFSISNSAED
510
PFIAIHAESK L
Length:511
Mass (Da):57,710
Last modified:February 1, 1991 - v1
Checksum:i05FC3B1EC1143857
GO
Isoform 2 (identifier: P19961-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     368-370: DVN → EHG
     371-511: Missing.

Note: No experimental confirmation available.
Show »
Length:370
Mass (Da):42,021
Checksum:i2E294536A637C8CB
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J2Z5C9J2Z5_HUMAN
Alpha-amylase 2B
AMY2B
96Annotation score:
C9JWK7C9JWK7_HUMAN
Alpha-amylase 2B
AMY2B
86Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056932368 – 370DVN → EHG in isoform 2. 1 Publication3
Alternative sequenceiVSP_056933371 – 511Missing in isoform 2. 1 PublicationAdd BLAST141

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24895 mRNA Translation: AAA35525.1
D90097 Genomic DNA Translation: BAA14130.1
AK095605 mRNA Translation: BAG53093.1
AK127047 mRNA Translation: BAG54429.1
AC105272 Genomic DNA No translation available.
CH471097 Genomic DNA Translation: EAW72902.1
CH471097 Genomic DNA Translation: EAW72903.1
CH471097 Genomic DNA Translation: EAW72904.1
BC011179 mRNA Translation: AAH11179.1
BC020861 mRNA Translation: AAH20861.1
X07057 Genomic DNA Translation: CAA30100.1
M18670 Genomic DNA Translation: AAA51725.1
CCDSiCCDS782.1 [P19961-1]
PIRiJS0165 ALHU2B
RefSeqiNP_066188.1, NM_020978.4 [P19961-1]
UniGeneiHs.484588

Genome annotation databases

EnsembliENST00000361355; ENSP00000354610; ENSG00000240038 [P19961-1]
ENST00000477657; ENSP00000433347; ENSG00000240038 [P19961-2]
ENST00000610648; ENSP00000481588; ENSG00000240038 [P19961-1]
GeneIDi280
KEGGihsa:280
UCSCiuc001duq.5 human [P19961-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24895 mRNA Translation: AAA35525.1
D90097 Genomic DNA Translation: BAA14130.1
AK095605 mRNA Translation: BAG53093.1
AK127047 mRNA Translation: BAG54429.1
AC105272 Genomic DNA No translation available.
CH471097 Genomic DNA Translation: EAW72902.1
CH471097 Genomic DNA Translation: EAW72903.1
CH471097 Genomic DNA Translation: EAW72904.1
BC011179 mRNA Translation: AAH11179.1
BC020861 mRNA Translation: AAH20861.1
X07057 Genomic DNA Translation: CAA30100.1
M18670 Genomic DNA Translation: AAA51725.1
CCDSiCCDS782.1 [P19961-1]
PIRiJS0165 ALHU2B
RefSeqiNP_066188.1, NM_020978.4 [P19961-1]
UniGeneiHs.484588

3D structure databases

ProteinModelPortaliP19961
SMRiP19961
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106777, 3 interactors
IntActiP19961, 2 interactors
STRINGi9606.ENSP00000354610

Chemistry databases

DrugBankiDB02730 4-Methylthio-Alpha-D-Mannose
DB03092 5-Hydroxymethyl-Chonduritol
DB03277 alpha-maltotriose
DB02379 Beta-D-Glucose
DB03323 Maltose
DB04417 P-Nitrophenol
DB03088 Pyroglutamic Acid

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

PTM databases

iPTMnetiP19961
PhosphoSitePlusiP19961

Polymorphism and mutation databases

DMDMi113789

Proteomic databases

MaxQBiP19961
PaxDbiP19961
PeptideAtlasiP19961
PRIDEiP19961
ProteomicsDBi53704
TopDownProteomicsiP19961-1 [P19961-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361355; ENSP00000354610; ENSG00000240038 [P19961-1]
ENST00000477657; ENSP00000433347; ENSG00000240038 [P19961-2]
ENST00000610648; ENSP00000481588; ENSG00000240038 [P19961-1]
GeneIDi280
KEGGihsa:280
UCSCiuc001duq.5 human [P19961-1]

Organism-specific databases

CTDi280
DisGeNETi280
EuPathDBiHostDB:ENSG00000240038.6
GeneCardsiAMY2B
HGNCiHGNC:478 AMY2B
HPAiHPA045394
HPA045399
HPA046980
MIMi104660 gene
neXtProtiNX_P19961
OpenTargetsiENSG00000240038
PharmGKBiPA24785
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
HOGENOMiHOG000253313
HOVERGENiHBG000061
InParanoidiP19961
KOiK01176
OMAiWKCQHAW
OrthoDBiEOG091G07V6
PhylomeDBiP19961
TreeFamiTF312850

Enzyme and pathway databases

ReactomeiR-HSA-189085 Digestion of dietary carbohydrate

Miscellaneous databases

ChiTaRSiAMY2B human
GeneWikiiAMY2B
GenomeRNAii280
PROiPR:P19961
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000240038 Expressed in 88 organ(s), highest expression level in body of pancreas
CleanExiHS_AMY2B
ExpressionAtlasiP19961 baseline and differential
GenevisibleiP19961 HS

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY2B_HUMAN
AccessioniPrimary (citable) accession number: P19961
Secondary accession number(s): B3KTI1
, B3KXB7, D3DT76, Q9UBH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: November 7, 2018
This is version 180 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again