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Protein

Enterobactin synthase component D

Gene

entD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron2+ atoms. Plays an essential role in the assembly of the enterobactin by catalyzing the transfer of the 4'-phosphopantetheine (Ppant) moiety from coenzyme A to the apo-domains of both EntB (ArCP domain) and EntF (PCP domain) to yield their holo-forms which make them competent for the activation of 2,3-dihydroxybenzoate (DHB) and L-serine, respectively.3 Publications

Miscellaneous

Deletion of the C-terminal 25 residues of EntB results in very strong decrease of the catalytic efficiency of EntD.1 Publication

Catalytic activityi

CoA + apo-EntB/F = adenosine 3',5'-bisphosphate + holo-EntB/F.2 Publications

Cofactori

Mg2+By similarity

Kineticsi

Kcat is 5.1 min(-1) for transferase activity with EntB as substrate (at pH 7.5 and 37 degrees Celsius).1 Publication
  1. KM=6.5 µM for EntB (at pH 7.5 and 37 degrees Celsius)1 Publication

    Pathwayi: enterobactin biosynthesis

    This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi107MagnesiumBy similarity1
    Metal bindingi109MagnesiumBy similarity1
    Metal bindingi152MagnesiumBy similarity1

    GO - Molecular functioni

    • holo-[acyl-carrier-protein] synthase activity Source: EcoCyc
    • magnesium ion binding Source: InterPro

    GO - Biological processi

    • enterobactin biosynthetic process Source: EcoCyc

    Keywordsi

    Molecular functionTransferase
    Biological processEnterobactin biosynthesis
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ENTD-MONOMER
    MetaCyc:ENTD-MONOMER
    UniPathwayi
    UPA00017

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enterobactin synthase component D1 Publication
    Alternative name(s):
    4'-phosphopantetheinyl transferase EntD1 Publication (EC:2.7.8.-2 Publications)
    Enterochelin synthase D1 Publication
    Gene namesi
    Name:entD1 Publication
    Ordered Locus Names:b0583, JW5085
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10262 entD

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002060691 – 206Enterobactin synthase component DAdd BLAST206

    Proteomic databases

    PaxDbiP19925
    PRIDEiP19925

    Interactioni

    Subunit structurei

    EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase.1 Publication

    Protein-protein interaction databases

    BioGridi4260709, 77 interactors
    IntActiP19925, 1 interactor
    STRINGi316385.ECDH10B_0650

    Structurei

    3D structure databases

    ProteinModelPortaliP19925
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4108TSE Bacteria
    COG2977 LUCA
    HOGENOMiHOG000276815
    InParanoidiP19925
    KOiK02362

    Family and domain databases

    Gene3Di3.90.470.20, 1 hit
    InterProiView protein in InterPro
    IPR008278 4-PPantetheinyl_Trfase_dom
    IPR037143 4-PPantetheinyl_Trfase_dom_sf
    IPR003542 Enbac_synth_compD-like
    PANTHERiPTHR38096 PTHR38096, 1 hit
    PfamiView protein in Pfam
    PF01648 ACPS, 1 hit
    PRINTSiPR01399 ENTSNTHTASED
    SUPFAMiSSF56214 SSF56214, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P19925-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKTTHTSLPF AGHTLHFVEF DPANFCEQDL LWLPHYAQLQ HAGRKRKTEH
    60 70 80 90 100
    LAGRIAAVYA LREYGYKCVP AIGELRQPVW PAEVYGSISH CGTTALAVVS
    110 120 130 140 150
    RQPIGIDIEE IFSVQTAREL TDNIITPAEH ERLADCGLAF SLALTLAFSA
    160 170 180 190 200
    KESAFKASEI QTDAGFLDYQ IISWNKQQVI IHRENEMFAV HWQIKEKIVI

    TLCQHD
    Length:206
    Mass (Da):23,259
    Last modified:November 16, 2011 - v3
    Checksum:iF2ED3F858A0C5DDF
    GO

    Sequence cautioni

    The sequence AAB40782 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA35224 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence CAB57861 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti74 – 75EL → DV in CAB57861 (PubMed:2526281).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X17426 Genomic DNA Translation: CAB57861.1 Different initiation.
    U82598 Genomic DNA Translation: AAB40782.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73684.2
    AP009048 Genomic DNA Translation: BAA35224.2 Different initiation.
    PIRiE64791
    RefSeqiNP_415115.2, NC_000913.3
    WP_001749708.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73684; AAC73684; b0583
    BAA35224; BAA35224; BAA35224
    GeneIDi945194
    KEGGiecj:JW5085
    eco:b0583
    PATRICifig|511145.12.peg.608

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X17426 Genomic DNA Translation: CAB57861.1 Different initiation.
    U82598 Genomic DNA Translation: AAB40782.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73684.2
    AP009048 Genomic DNA Translation: BAA35224.2 Different initiation.
    PIRiE64791
    RefSeqiNP_415115.2, NC_000913.3
    WP_001749708.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP19925
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260709, 77 interactors
    IntActiP19925, 1 interactor
    STRINGi316385.ECDH10B_0650

    Proteomic databases

    PaxDbiP19925
    PRIDEiP19925

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73684; AAC73684; b0583
    BAA35224; BAA35224; BAA35224
    GeneIDi945194
    KEGGiecj:JW5085
    eco:b0583
    PATRICifig|511145.12.peg.608

    Organism-specific databases

    EchoBASEiEB0258
    EcoGeneiEG10262 entD

    Phylogenomic databases

    eggNOGiENOG4108TSE Bacteria
    COG2977 LUCA
    HOGENOMiHOG000276815
    InParanoidiP19925
    KOiK02362

    Enzyme and pathway databases

    UniPathwayi
    UPA00017

    BioCyciEcoCyc:ENTD-MONOMER
    MetaCyc:ENTD-MONOMER

    Miscellaneous databases

    PROiPR:P19925

    Family and domain databases

    Gene3Di3.90.470.20, 1 hit
    InterProiView protein in InterPro
    IPR008278 4-PPantetheinyl_Trfase_dom
    IPR037143 4-PPantetheinyl_Trfase_dom_sf
    IPR003542 Enbac_synth_compD-like
    PANTHERiPTHR38096 PTHR38096, 1 hit
    PfamiView protein in Pfam
    PF01648 ACPS, 1 hit
    PRINTSiPR01399 ENTSNTHTASED
    SUPFAMiSSF56214 SSF56214, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiENTD_ECOLI
    AccessioniPrimary (citable) accession number: P19925
    Secondary accession number(s): P77092
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
    Last sequence update: November 16, 2011
    Last modified: April 25, 2018
    This is version 140 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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