entD

Functionⁱ

Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron²⁺ atoms. Plays an essential role in the assembly of the enterobactin by catalyzing the transfer of the 4'-phosphopantetheine (Ppant) moiety from coenzyme A to the apo-domains of both EntB (ArCP domain) and EntF (PCP domain) to yield their holo-forms which make them competent for the activation of 2,3-dihydroxybenzoate (DHB) and L-serine, respectively.3 Publications

Miscellaneous

Deletion of the C-terminal 25 residues of EntB results in very strong decrease of the catalytic efficiency of EntD.1 Publication

Catalytic activityⁱ

CoA + apo-EntB/F = adenosine 3',5'-bisphosphate + holo-EntB/F.2 Publications

Cofactorⁱ

Mg²⁺By similarity

Kineticsⁱ

Kcat is 5.1 min(-1) for transferase activity with EntB as substrate (at pH 7.5 and 37 degrees Celsius).1 Publication

K_M=
6.5 µM for EntB (at pH 7.5 and 37 degrees Celsius)
1 Publication
Manual assertion based on experiment inⁱ
- Ref.8
  "Enterobactin biosynthesis in Escherichia coli: isochorismate lyase (EntB) is a bifunctional enzyme that is phosphopantetheinylated by EntD and then acylated by EntE using ATP and 2,3-dihydroxybenzoate."
  Gehring A.M., Bradley K.A., Walsh C.T.
  Biochemistry 36:8495-8503(1997) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.

Pathwayⁱ: enterobactin biosynthesis

This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	107	MagnesiumBy similarity	1
Metal bindingⁱ	109	MagnesiumBy similarity	1
Metal bindingⁱ	152	MagnesiumBy similarity	1

GO - Molecular functionⁱ

holo-[acyl-carrier-protein] synthase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 9214294
- 9485415
magnesium ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

enterobactin biosynthetic process
Source: EcoCyc
Inferred from mutant phenotypeⁱ
- 4939766

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Enterobactin biosynthesis
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:ENTD-MONOMER MetaCyc:ENTD-MONOMER
UniPathwayⁱ	UPA00017

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Enterobactin synthase component D1 Publication Alternative name(s): 4'-phosphopantetheinyl transferase EntD1 Publication (EC:2.7.8.-2 Publications) Enterochelin synthase D1 Publication
Gene namesⁱ	Name:entD1 Publication Ordered Locus Names:b0583, JW5085
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10262 entD

EcoGeneⁱ

EG10262 entD

Subcellular locationⁱ

Membrane
1 Publication
Manual assertion based on experiment inⁱ
- Ref.2
  "The Escherichia coli enterobactin biosynthesis gene, entD: nucleotide sequence and membrane localization of its protein product."
  Armstrong S.K., Pettis G.S., Forrester L.J., McIntosh M.A.
  Mol. Microbiol. 3:757-766(1989) [PubMed] [Europe PMC] [Abstract]
  Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION.

GO - Cellular componentⁱ

enterobactin synthetase complex
Source: EcoCyc
Inferred from direct assayⁱ
- 9485415
intrinsic component of plasma membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 2526281

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000206069	1 – 206	Enterobactin synthase component DAdd BLAST		206

Proteomic databases

PaxDbⁱ	P19925
PRIDEⁱ	P19925

Interactionⁱ

Subunit structureⁱ

EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase.1 Publication

Protein-protein interaction databases

BioGridⁱ	4260709, 77 interactors
IntActⁱ	P19925, 1 interactor
STRINGⁱ	316385.ECDH10B_0650

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P19925
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the P-Pant transferase superfamily. EntD family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4108TSE Bacteria COG2977 LUCA
HOGENOMⁱ	HOG000276815
InParanoidⁱ	P19925
KEGG Orthology (KO) More...KOⁱ	K02362

Family and domain databases

Gene3Dⁱ	3.90.470.20, 1 hit
InterProⁱ	View protein in InterPro IPR008278 4-PPantetheinyl_Trfase_dom IPR037143 4-PPantetheinyl_Trfase_dom_sf IPR003542 Enbac_synth_compD-like
PANTHERⁱ	PTHR38096 PTHR38096, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01648 ACPS, 1 hit
PRINTSⁱ	PR01399 ENTSNTHTASED
SUPFAMⁱ	SSF56214 SSF56214, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P19925-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKTTHTSLPF AGHTLHFVEF DPANFCEQDL LWLPHYAQLQ HAGRKRKTEH 
        60         70         80         90        100
LAGRIAAVYA LREYGYKCVP AIGELRQPVW PAEVYGSISH CGTTALAVVS 
       110        120        130        140        150
RQPIGIDIEE IFSVQTAREL TDNIITPAEH ERLADCGLAF SLALTLAFSA 
       160        170        180        190        200
KESAFKASEI QTDAGFLDYQ IISWNKQQVI IHRENEMFAV HWQIKEKIVI 

TLCQHD

Length:206

Mass (Da):23,259

Last modified:November 16, 2011 - v3

Checksum:ⁱF2ED3F858A0C5DDF

GO

Sequence cautionⁱ

The sequence AAB40782 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence BAA35224 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

The sequence CAB57861 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	74 – 75	EL → DV in CAB57861 (PubMed:2526281).Curated		2

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X17426 Genomic DNA Translation: CAB57861.1 Different initiation. U82598 Genomic DNA Translation: AAB40782.1 Different initiation. U00096 Genomic DNA Translation: AAC73684.2 AP009048 Genomic DNA Translation: BAA35224.2 Different initiation.
PIRⁱ	E64791
NCBI Reference Sequences More...RefSeqⁱ	NP_415115.2, NC_000913.3 WP_001749708.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC73684; AAC73684; b0583 BAA35224; BAA35224; BAA35224
GeneIDⁱ	945194
KEGGⁱ	ecj:JW5085 eco:b0583
PATRICⁱ	fig\|511145.12.peg.608

Protein	Similar proteins		Species	Score	Length	Source
P19925	EntD		ECOLX		256	UniRef100_P19925
4'-phosphopantetheinyl transferase EntD		Escherichia coli H386		256
Enterochelin synthetase, component D		Escherichia coli chi7122		256
4'-phosphopantetheinyl transferase EntD (Enterobactinsynthetase component D) (Enterochelin synthase D)		Escherichia coli H736		256
Enterobactin synthase subunit EntD (Fragment)		Staphylococcus succinus		74
+27

Protein	Similar proteins		Species	Score	Length	Source
P19925	Enterobactin synthase component D		ECO57		206	UniRef90_P19925
Enterobactin synthase component D		SHIFL		206
EntD		ECOLX		256
4'-phosphopantetheinyl transferase EntD		Escherichia coli H386		256
Enterochelin synthetase, component D		Escherichia coli chi7122		256
+184

Protein	Similar proteins		Species	Score	Length	Source
P19925	Enterobactin synthase component D		ECO57		206	UniRef50_P19925
Enterobactin synthase component D		SHIFL		206
EntD		ECOLX		256
4'-phosphopantetheinyl transferase EntD		Escherichia coli H386		256
Enterochelin synthetase, component D		Escherichia coli chi7122		256
+614

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	X17426 Genomic DNA Translation: CAB57861.1 Different initiation. U82598 Genomic DNA Translation: AAB40782.1 Different initiation. U00096 Genomic DNA Translation: AAC73684.2 AP009048 Genomic DNA Translation: BAA35224.2 Different initiation.
PIRⁱ	E64791
RefSeqⁱ	NP_415115.2, NC_000913.3 WP_001749708.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P19925
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4260709, 77 interactors
IntActⁱ	P19925, 1 interactor
STRINGⁱ	316385.ECDH10B_0650

Proteomic databases

PaxDbⁱ	P19925
PRIDEⁱ	P19925

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC73684; AAC73684; b0583 BAA35224; BAA35224; BAA35224
GeneIDⁱ	945194
KEGGⁱ	ecj:JW5085 eco:b0583
PATRICⁱ	fig\|511145.12.peg.608

Organism-specific databases

EchoBASEⁱ	EB0258
EcoGeneⁱ	EG10262 entD

Phylogenomic databases

eggNOGⁱ	ENOG4108TSE Bacteria COG2977 LUCA
HOGENOMⁱ	HOG000276815
InParanoidⁱ	P19925
KOⁱ	K02362

Enzyme and pathway databases

UniPathwayⁱ	UPA00017
BioCycⁱ	EcoCyc:ENTD-MONOMER MetaCyc:ENTD-MONOMER

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P19925

PROⁱ

PR:P19925

Family and domain databases

Gene3Dⁱ	3.90.470.20, 1 hit
InterProⁱ	View protein in InterPro IPR008278 4-PPantetheinyl_Trfase_dom IPR037143 4-PPantetheinyl_Trfase_dom_sf IPR003542 Enbac_synth_compD-like
PANTHERⁱ	PTHR38096 PTHR38096, 1 hit
Pfamⁱ	View protein in Pfam PF01648 ACPS, 1 hit
PRINTSⁱ	PR01399 ENTSNTHTASED
SUPFAMⁱ	SSF56214 SSF56214, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ENTD_ECOLI
Accessionⁱ	P19925Primary (citable) accession number: P19925 Secondary accession number(s): P77092
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 1, 1991
	Last sequence update:	November 16, 2011
	Last modified:	April 25, 2018
	This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P19925 (ENTD_ECOLI)

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Enterobactin synthase component D

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: enterobactin biosynthesis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Kineticsⁱ

Pathwayⁱ: enterobactin biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ