UniProtKB - P19925 (ENTD_ECOLI)
Enterobactin synthase component D
entD
Functioni
Involved in the biosynthesis of the siderophore enterobactin (enterochelin), which is a macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine. The serine trilactone serves as a scaffolding for the three catechol functionalities that provide hexadentate coordination for the tightly ligated iron2+ atoms. Plays an essential role in the assembly of the enterobactin by catalyzing the transfer of the 4'-phosphopantetheine (Ppant) moiety from coenzyme A to the apo-domains of both EntB (ArCP domain) and EntF (PCP domain) to yield their holo-forms which make them competent for the activation of 2,3-dihydroxybenzoate (DHB) and L-serine, respectively.
3 PublicationsMiscellaneous
Catalytic activityi
- apo-[aryl-carrier protein] + CoA = adenosine 3',5'-bisphosphate + H+ + holo-[aryl-carrier protein]2 Publications
- apo-[peptidyl-carrier protein] + CoA = adenosine 3',5'-bisphosphate + H+ + holo-[peptidyl-carrier protein]2 Publications
Cofactori
Kineticsi
- KM=6.5 µM for EntB (at pH 7.5 and 37 degrees Celsius)1 Publication
: enterobactin biosynthesis Pathwayi
This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 107 | MagnesiumBy similarity | 1 | |
Metal bindingi | 109 | MagnesiumBy similarity | 1 | |
Metal bindingi | 152 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- holo-[acyl-carrier-protein] synthase activity Source: EcoCyc
- magnesium ion binding Source: InterPro
GO - Biological processi
- enterobactin biosynthetic process Source: EcoCyc
- siderophore metabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Enterobactin biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:ENTD-MONOMER |
BRENDAi | 2.7.8.7, 2026 |
UniPathwayi | UPA00017 |
Names & Taxonomyi
Protein namesi | Recommended name: Enterobactin synthase component D1 PublicationAlternative name(s): 4'-phosphopantetheinyl transferase EntD1 Publication (EC:2.7.8.-2 Publications) Enterochelin synthase D1 Publication |
Gene namesi | Name:entD1 Publication Ordered Locus Names:b0583, JW5085 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Membrane 1 Publication
Plasma Membrane
- intrinsic component of plasma membrane Source: EcoliWiki
Other locations
- enterobactin synthetase complex Source: EcoCyc
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000206069 | 1 – 206 | Enterobactin synthase component DAdd BLAST | 206 |
Proteomic databases
PaxDbi | P19925 |
PRIDEi | P19925 |
Interactioni
Subunit structurei
EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260709, 77 interactors 849580, 1 interactor |
IntActi | P19925, 1 interactor |
STRINGi | 511145.b0583 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG2977, Bacteria |
HOGENOMi | CLU_075076_1_0_6 |
InParanoidi | P19925 |
PhylomeDBi | P19925 |
Family and domain databases
Gene3Di | 3.90.470.20, 1 hit |
InterProi | View protein in InterPro IPR008278, 4-PPantetheinyl_Trfase_dom IPR037143, 4-PPantetheinyl_Trfase_dom_sf IPR041354, 4PPT_N IPR003542, Enbac_synth_compD-like |
PANTHERi | PTHR38096, PTHR38096, 1 hit |
Pfami | View protein in Pfam PF17837, 4PPT_N, 1 hit PF01648, ACPS, 1 hit |
PRINTSi | PR01399, ENTSNTHTASED |
SUPFAMi | SSF56214, SSF56214, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKTTHTSLPF AGHTLHFVEF DPANFCEQDL LWLPHYAQLQ HAGRKRKTEH
60 70 80 90 100
LAGRIAAVYA LREYGYKCVP AIGELRQPVW PAEVYGSISH CGTTALAVVS
110 120 130 140 150
RQPIGIDIEE IFSVQTAREL TDNIITPAEH ERLADCGLAF SLALTLAFSA
160 170 180 190 200
KESAFKASEI QTDAGFLDYQ IISWNKQQVI IHRENEMFAV HWQIKEKIVI
TLCQHD
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 74 – 75 | EL → DV in CAB57861 (PubMed:2526281).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17426 Genomic DNA Translation: CAB57861.1 Different initiation. U82598 Genomic DNA Translation: AAB40782.1 Different initiation. U00096 Genomic DNA Translation: AAC73684.2 AP009048 Genomic DNA Translation: BAA35224.2 Different initiation. |
PIRi | E64791 |
RefSeqi | NP_415115.2, NC_000913.3 WP_001749708.1, NZ_STEB01000047.1 |
Genome annotation databases
EnsemblBacteriai | AAC73684; AAC73684; b0583 BAA35224; BAA35224; BAA35224 |
GeneIDi | 945194 |
KEGGi | ecj:JW5085 eco:b0583 |
PATRICi | fig|511145.12.peg.608 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X17426 Genomic DNA Translation: CAB57861.1 Different initiation. U82598 Genomic DNA Translation: AAB40782.1 Different initiation. U00096 Genomic DNA Translation: AAC73684.2 AP009048 Genomic DNA Translation: BAA35224.2 Different initiation. |
PIRi | E64791 |
RefSeqi | NP_415115.2, NC_000913.3 WP_001749708.1, NZ_STEB01000047.1 |
3D structure databases
SMRi | P19925 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260709, 77 interactors 849580, 1 interactor |
IntActi | P19925, 1 interactor |
STRINGi | 511145.b0583 |
Proteomic databases
PaxDbi | P19925 |
PRIDEi | P19925 |
Genome annotation databases
EnsemblBacteriai | AAC73684; AAC73684; b0583 BAA35224; BAA35224; BAA35224 |
GeneIDi | 945194 |
KEGGi | ecj:JW5085 eco:b0583 |
PATRICi | fig|511145.12.peg.608 |
Organism-specific databases
EchoBASEi | EB0258 |
Phylogenomic databases
eggNOGi | COG2977, Bacteria |
HOGENOMi | CLU_075076_1_0_6 |
InParanoidi | P19925 |
PhylomeDBi | P19925 |
Enzyme and pathway databases
UniPathwayi | UPA00017 |
BioCyci | EcoCyc:ENTD-MONOMER |
BRENDAi | 2.7.8.7, 2026 |
Miscellaneous databases
PROi | PR:P19925 |
Family and domain databases
Gene3Di | 3.90.470.20, 1 hit |
InterProi | View protein in InterPro IPR008278, 4-PPantetheinyl_Trfase_dom IPR037143, 4-PPantetheinyl_Trfase_dom_sf IPR041354, 4PPT_N IPR003542, Enbac_synth_compD-like |
PANTHERi | PTHR38096, PTHR38096, 1 hit |
Pfami | View protein in Pfam PF17837, 4PPT_N, 1 hit PF01648, ACPS, 1 hit |
PRINTSi | PR01399, ENTSNTHTASED |
SUPFAMi | SSF56214, SSF56214, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENTD_ECOLI | |
Accessioni | P19925Primary (citable) accession number: P19925 Secondary accession number(s): P77092 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | November 16, 2011 | |
Last modified: | February 23, 2022 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families