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Protein

Viral transcription factor IE2

Gene

UL122

Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Stimulates viral early and late gene expression and thus play a crucial role in the regulation of productive infection. In addition, activates quiescent cells to reenter the cell cycle and upregulates several E2F-responsive genes, which are responsible for pushing the cell into S phase. In S-phase, inhibits cellular DNA synthesis and blocks further cell cycle progression.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processG0/G1 host cell cycle checkpoint dysregulation by virus, G1/S host cell cycle checkpoint dysregulation by virus, Host-virus interaction, Modulation of host cell cycle by virus, Transcription, Transcription regulation
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Viral transcription factor IE2
Short name:
IE2
Alternative name(s):
Protein UL122
Gene namesi
Name:UL122
OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri10360 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008992 Componenti: Genome
  • UP000008991 Componenti: Genome

Subcellular locationi

  • Host nucleus
  • Note: Colocalizes with host PML-associated nuclear bodies.

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001153511 – 580Viral transcription factor IE2Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki175Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Cross-linki180Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Post-translational modificationi

Sumoylated. The sumoylation is necessary for efficient replication of the virus and thus for the function of this viral transcription factor.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PRIDEiP19893

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with host SUMO-modified form of TATA-binding protein (TBP)-associated factor 12/TAF12 in a SIM-dependent manner; this interaction increases the transactivation activity of IE2. Interacts with host CHAF1A. Interacts with several components of the host transcriptional machinery including TBP, TF2B and CREB1. Interacts with host DNA replication licensing factor MCM3.3 Publications

Protein-protein interaction databases

ELMiP19893

Structurei

3D structure databases

SMRiP19893
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni200 – 208Non-covalent SUMO1 binding region (SIM)9

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi258 – 320Gly/Ser-richAdd BLAST63
Compositional biasi258 – 276Poly-SerAdd BLAST19

Domaini

The SUMO-interacting motif (SIM) is required for efficient transactivation function.1 Publication

Sequence similaritiesi

Belongs to the HHV-5 IE2 protein family.Curated

Phylogenomic databases

OrthoDBiVOG0900005Z

Family and domain databases

InterProiView protein in InterPro
IPR010855 Cytomega_IE1
IPR005028 Herpes_IE2_3
PfamiView protein in Pfam
PF07340 Herpes_IE1, 1 hit
PF03361 Herpes_IE2_3, 1 hit

Sequencei

Sequence statusi: Complete.

P19893-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESSAKRKMD PDNPDEGPSS KVPRPETPVT KATTFLQTML RKEVNSQLSL
60 70 80 90 100
GDPLFPELAE ESLKTFEQVT EDCNENPEKD VLAELGDILA QAVNHAGIDS
110 120 130 140 150
SSTGPTLTTH SCSVSSAPLN KPTPTSVAVT NTPLPGASAT PELSPRKKPR
160 170 180 190 200
KTTRPFKVII KPPVPPAPIM LPLIKQEDIK PEPDFTIQYR NKIIDTAGCI
210 220 230 240 250
VISDSEEEQG EEVETRGATA SSPSTGSGTP RVTSPTHPLS QMNHPPLPDP
260 270 280 290 300
LGRPDEDSSS SSSSSCSSAS DSESESEEMK CSSGGGASVT SSHHGRGGFG
310 320 330 340 350
GAASSSLLSC GHQSSGGAST GPRKKKSKRI SELDNEKVRN IMKDKNTPFC
360 370 380 390 400
TPNVQTRRGR VKIDEVSRMF RNTNRSLEYK NLPFTIPSMH QVLDEAIKAC
410 420 430 440 450
KTMQVNNKGI QIIYTRNHEV KSEVDAVRCR LGTMCNLALS TPFLMEHTMP
460 470 480 490 500
VTHPPEVAQR TADACNEGVK AAWSLKELHT HQLCPRSSDY RNMIIHAATP
510 520 530 540 550
VDLLGALNLC LPLMQKFPKQ VMVRIFSTNQ GGFMLPIYET AAKAYAVGQF
560 570 580
EQPTETPPED LDTLSLAIEA AIQDLRNKSQ
Length:580
Mass (Da):62,875
Last modified:February 15, 2005 - v2
Checksum:i41D1753A02A077ED
GO

Sequence cautioni

The sequence CAA35324 differs from that shown. Reason: Frameshift at positions 25 and 87.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17403 Genomic DNA Translation: CAA35324.1 Frameshift.
BK000394 Genomic DNA Translation: DAA00111.1
PIRiS09889 EDBEM4

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17403 Genomic DNA Translation: CAA35324.1 Frameshift.
BK000394 Genomic DNA Translation: DAA00111.1
PIRiS09889 EDBEM4

3D structure databases

SMRiP19893
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ELMiP19893

Proteomic databases

PRIDEiP19893

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG0900005Z

Family and domain databases

InterProiView protein in InterPro
IPR010855 Cytomega_IE1
IPR005028 Herpes_IE2_3
PfamiView protein in Pfam
PF07340 Herpes_IE1, 1 hit
PF03361 Herpes_IE2_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiVIE2_HCMVA
AccessioniPrimary (citable) accession number: P19893
Secondary accession number(s): Q7M6S5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 15, 2005
Last modified: November 22, 2017
This is version 78 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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