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UniProtKB - P19822 (DPOL_BPT5)
Protein
DNA polymerase
Gene
T5.122
Organism
Escherichia phage T5 (Enterobacteria phage T5)
Status
Functioni
Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'-5' direction for proofreading purpose. The DNA synthesis very likely occurs by strand displacement.
2 PublicationsCatalytic activityi
- EC:2.7.7.71 Publication
GO - Molecular functioni
- 3'-5' exonuclease activity Source: UniProtKB
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB
GO - Biological processi
- DNA-dependent DNA replication Source: InterPro
- viral DNA genome replication Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA replication, Viral DNA replication |
Names & Taxonomyi
Protein namesi | |
Gene namesi | ORF Names:T5.122, T5p120Imported |
Organismi | Escherichia phage T5 (Enterobacteria phage T5) |
Taxonomic identifieri | 2695836 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Demerecviridae › Markadamsvirinae › Tequintavirus › |
Virus hosti | Escherichia coli [TaxID: 562] |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101267 | 1 – 855 | DNA polymeraseAdd BLAST | 855 |
Expressioni
Inductioni
Expressed in the early phase of the viral replicative cycle.1 Publication
Keywords - Developmental stagei
Early proteinInteractioni
Subunit structurei
Single-chain monomer with multiple functions.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 107 – 332 | 3'-5' exonucleaseSequence analysisAdd BLAST | 226 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 333 – 833 | Polymerase1 PublicationAdd BLAST | 501 |
Domaini
The C-terminus seems to increase the affinity of the DNA for the polymerase active site, and thus increase processivity and decrease the accessibility of the DNA to the exonuclease active site.1 Publication
Sequence similaritiesi
Belongs to the DNA polymerase type-A family.Curated
Family and domain databases
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR002298, DNA_polymerase_A IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF00476, DNA_pol_A, 1 hit PF01612, DNA_pol_A_exo1, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
i Sequence
Sequence statusi: Complete.
P19822-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIAVVDKAL NNTRYDKHFQ LYGEEVDVFH MCNEKLSGRL LKKHITIGTP
60 70 80 90 100
ENPFDPNDYD FVILVGAEPF LYFAGKKGIG DYTGKRVEYN GYANWIASIS
110 120 130 140 150
PAQLHFKPEM KPVFDATVEN IHDIINGREK IAKAGDYRPI TDPDEAEEYI
160 170 180 190 200
KMVYNMVIGP VAFDSETSAL YCRDGYLLGV SISHQEYQGV YIDSDCLTEV
210 220 230 240 250
AVYYLQKILD SENHTIVFHN LKFDMHFYKY HLGLTFDKAH KERRLHDTML
260 270 280 290 300
QHYVLDERRG THGLKSLAMK YTDMGDYDFE LDKFKDDYCK AHKIKKEDFT
310 320 330 340 350
YDLIPFDIMW PYAAKDTDAT IRLHNFFLPK IEKNEKLCSL YYDVLMPGCV
360 370 380 390 400
FLQRVEDRGV PISIDRLKEA QYQLTHNLNK AREKLYTYPE VKQLEQDQNE
410 420 430 440 450
AFNPNSVKQL RVLLFDYVGL TPTGKLTDTG ADSTDAEALN ELATQHPIAK
460 470 480 490 500
TLLEIRKLTK LISTYVEKIL LSIDADGCIR TGFHEHMTTS GRLSSSGKLN
510 520 530 540 550
LQQLPRDESI IKGCVVAPPG YRVIAWDLTT AEVYYAAVLS GDRNMQQVFI
560 570 580 590 600
NMRNEPDKYP DFHSNIAHMV FKLQCEPRDV KKLFPALRQA AKAITFGILY
610 620 630 640 650
GSGPAKVAHS VNEALLEQAA KTGEPFVECT VADAKEYIET YFGQFPQLKR
660 670 680 690 700
WIDKCHDQIK NHGFIYSHFG RKRRLHNIHS EDRGVQGEEI RSGFNAIIQS
710 720 730 740 750
ASSDSLLLGA VDADNEIISL GLEQEMKIVM LVHDSVVAIV REDLIDQYNE
760 770 780 790 800
ILIRNIQKDR GISIPGCPIG IDSDSEAGGS RDYSCGKMKK QHPSIACIDD
810 820 830 840 850
DEYTRYVKGV LLDAEFEYKK LAAMDKEHPD HSKYKDDKFI AVCKDLDNVK
RILGA
Sequence cautioni
The sequence AAA32558 differs from that shown.Curated
The sequence AAA32561 differs from that shown. Reason: Frameshift.Curated
The sequence AAX12050 differs from that shown.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24354 Genomic DNA Translation: AAA32561.1 Frameshift. AY587007 Genomic DNA Translation: AAX12050.1 Different initiation. AY692264 Genomic DNA Translation: AAU05259.1 M64047 Genomic DNA Translation: AAA32558.1 Different initiation. |
PIRi | A33923, DJBPT5 |
RefSeqi | YP_006950.1, NC_005859.1 |
Genome annotation databases
GeneIDi | 2777603 |
KEGGi | vg:2777603 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24354 Genomic DNA Translation: AAA32561.1 Frameshift. AY587007 Genomic DNA Translation: AAX12050.1 Different initiation. AY692264 Genomic DNA Translation: AAU05259.1 M64047 Genomic DNA Translation: AAA32558.1 Different initiation. |
PIRi | A33923, DJBPT5 |
RefSeqi | YP_006950.1, NC_005859.1 |
3D structure databases
SMRi | P19822 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 2777603 |
KEGGi | vg:2777603 |
Family and domain databases
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR002298, DNA_polymerase_A IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF00476, DNA_pol_A, 1 hit PF01612, DNA_pol_A_exo1, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPOL_BPT5 | |
Accessioni | P19822Primary (citable) accession number: P19822 Secondary accession number(s): Q38546 Q6QGG5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | July 6, 2016 | |
Last modified: | May 25, 2022 | |
This is version 111 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- SIMILARITY comments
Index of protein domains and families