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UniProtKB - P19821 (DPO1_THEAQ)
Protein
DNA polymerase I, thermostable
Gene
polA
Organism
Thermus aquaticus
Status
Functioni
Catalytic activityi
- EC:2.7.7.7
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
- nucleoside binding Source: InterPro
GO - Biological processi
- DNA-dependent DNA replication Source: InterPro
- DNA repair Source: UniProtKB-KW
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6334 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:polA Synonyms:pol1 |
Organismi | Thermus aquaticus |
Taxonomic identifieri | 271 [NCBI] |
Taxonomic lineagei | Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus |
Pathology & Biotechi
Biotechnological usei
Used in the PCR method because of its high thermostability. Has a relatively high error rate because it lacks exonuclease proofreading functionality.
Chemistry databases
ChEMBLi | CHEMBL3564 |
DrugBanki | DB03258, 2'-Deoxycytidine 5'-triphosphate DB03152, B-2-Octylglucoside |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101256 | 1 – 832 | DNA polymerase I, thermostableAdd BLAST | 832 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P19821 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P19821 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 175 – 261 | 5'-3' exonucleaseSequence analysisAdd BLAST | 87 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 410 – 832 | PolymeraseBy similarityAdd BLAST | 423 |
Sequence similaritiesi
Belongs to the DNA polymerase type-A family.Curated
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf IPR015361, Taq_pol_thermo_exonuc |
PANTHERi | PTHR10133, PTHR10133, 2 hits |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit PF09281, Taq-exonuc, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00475, 53EXOc, 1 hit SM00278, HhH1, 2 hits SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
i Sequence
Sequence statusi: Complete.
P19821-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRGMLPLFEP KGRVLLVDGH HLAYRTFHAL KGLTTSRGEP VQAVYGFAKS
60 70 80 90 100
LLKALKEDGD AVIVVFDAKA PSFRHEAYGG YKAGRAPTPE DFPRQLALIK
110 120 130 140 150
ELVDLLGLAR LEVPGYEADD VLASLAKKAE KEGYEVRILT ADKDLYQLLS
160 170 180 190 200
DRIHVLHPEG YLITPAWLWE KYGLRPDQWA DYRALTGDES DNLPGVKGIG
210 220 230 240 250
EKTARKLLEE WGSLEALLKN LDRLKPAIRE KILAHMDDLK LSWDLAKVRT
260 270 280 290 300
DLPLEVDFAK RREPDRERLR AFLERLEFGS LLHEFGLLES PKALEEAPWP
310 320 330 340 350
PPEGAFVGFV LSRKEPMWAD LLALAAARGG RVHRAPEPYK ALRDLKEARG
360 370 380 390 400
LLAKDLSVLA LREGLGLPPG DDPMLLAYLL DPSNTTPEGV ARRYGGEWTE
410 420 430 440 450
EAGERAALSE RLFANLWGRL EGEERLLWLY REVERPLSAV LAHMEATGVR
460 470 480 490 500
LDVAYLRALS LEVAEEIARL EAEVFRLAGH PFNLNSRDQL ERVLFDELGL
510 520 530 540 550
PAIGKTEKTG KRSTSAAVLE ALREAHPIVE KILQYRELTK LKSTYIDPLP
560 570 580 590 600
DLIHPRTGRL HTRFNQTATA TGRLSSSDPN LQNIPVRTPL GQRIRRAFIA
610 620 630 640 650
EEGWLLVALD YSQIELRVLA HLSGDENLIR VFQEGRDIHT ETASWMFGVP
660 670 680 690 700
REAVDPLMRR AAKTINFGVL YGMSAHRLSQ ELAIPYEEAQ AFIERYFQSF
710 720 730 740 750
PKVRAWIEKT LEEGRRRGYV ETLFGRRRYV PDLEARVKSV REAAERMAFN
760 770 780 790 800
MPVQGTAADL MKLAMVKLFP RLEEMGARML LQVHDELVLE APKERAEAVA
810 820 830
RLAKEVMEGV YPLAVPLEVE VGIGEDWLSA KE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 155 | V → A in BAA06775 (PubMed:7896728).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04639 Genomic DNA Translation: AAA27507.1 D32013 Genomic DNA Translation: BAA06775.1 |
PIRi | A33530 JX0359 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04639 Genomic DNA Translation: AAA27507.1 D32013 Genomic DNA Translation: BAA06775.1 |
PIRi | A33530 JX0359 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BGX | X-ray | 2.30 | T | 1-832 | [»] | |
1JXE | X-ray | 2.85 | A | 293-832 | [»] | |
1KTQ | X-ray | 2.50 | A | 290-832 | [»] | |
1QSS | X-ray | 2.30 | A | 293-831 | [»] | |
1QSY | X-ray | 2.30 | A | 293-831 | [»] | |
1QTM | X-ray | 2.30 | A | 293-831 | [»] | |
1TAQ | X-ray | 2.40 | A | 1-832 | [»] | |
1TAU | X-ray | 3.00 | A | 1-832 | [»] | |
2KTQ | X-ray | 2.30 | A | 295-832 | [»] | |
3KTQ | X-ray | 2.30 | A | 293-832 | [»] | |
3LWL | X-ray | 2.25 | A | 293-832 | [»] | |
3LWM | X-ray | 2.19 | A | 293-832 | [»] | |
3M8R | X-ray | 2.00 | A | 293-832 | [»] | |
3M8S | X-ray | 2.20 | A | 293-832 | [»] | |
3OJS | X-ray | 1.90 | A | 293-832 | [»] | |
3OJU | X-ray | 2.00 | A | 293-832 | [»] | |
3PO4 | X-ray | 1.80 | A | 293-832 | [»] | |
3PO5 | X-ray | 2.39 | A | 293-832 | [»] | |
3PY8 | X-ray | 1.74 | A | 293-832 | [»] | |
3RR7 | X-ray | 1.95 | A | 293-832 | [»] | |
3RR8 | X-ray | 2.40 | A | 293-832 | [»] | |
3RRG | X-ray | 2.30 | A | 293-832 | [»] | |
3RRH | X-ray | 1.80 | A | 293-832 | [»] | |
3RTV | X-ray | 1.90 | A | 293-832 | [»] | |
3SV3 | X-ray | 2.10 | A | 293-832 | [»] | |
3SV4 | X-ray | 1.99 | A | 293-832 | [»] | |
3SYZ | X-ray | 1.95 | A | 293-832 | [»] | |
3SZ2 | X-ray | 2.15 | A | 293-832 | [»] | |
3T3F | X-ray | 1.90 | A | 293-832 | [»] | |
4BWJ | X-ray | 1.55 | A | 293-832 | [»] | |
4BWM | X-ray | 1.75 | A | 293-832 | [»] | |
4C8K | X-ray | 2.17 | A | 293-832 | [»] | |
4C8L | X-ray | 1.70 | A | 293-832 | [»] | |
4C8M | X-ray | 1.57 | A | 293-832 | [»] | |
4C8N | X-ray | 1.88 | A | 293-832 | [»] | |
4C8O | X-ray | 1.75 | A | 293-832 | [»] | |
4CCH | X-ray | 2.55 | A | 293-832 | [»] | |
4DF4 | X-ray | 2.20 | A | 293-832 | [»] | |
4DF8 | X-ray | 2.00 | A | 293-832 | [»] | |
4DFJ | X-ray | 1.90 | A | 293-832 | [»] | |
4DFK | X-ray | 1.65 | A | 293-832 | [»] | |
4DFM | X-ray | 1.89 | A | 293-832 | [»] | |
4DFP | X-ray | 2.00 | A | 293-832 | [»] | |
4DLE | X-ray | 2.44 | A | 293-832 | [»] | |
4DLG | X-ray | 1.89 | A | 293-832 | [»] | |
4ELT | X-ray | 2.20 | A | 293-832 | [»] | |
4ELU | X-ray | 1.80 | A | 293-832 | [»] | |
4ELV | X-ray | 1.90 | A | 293-832 | [»] | |
4KTQ | X-ray | 2.50 | A | 294-832 | [»] | |
4N56 | X-ray | 2.20 | A | 281-832 | [»] | |
4N5S | X-ray | 1.67 | A | 281-832 | [»] | |
4XIU | X-ray | 2.50 | A | 294-832 | [»] | |
5E41 | X-ray | 1.80 | A | 293-832 | [»] | |
5KTQ | X-ray | 2.50 | A | 290-832 | [»] | |
5NKL | X-ray | 1.70 | A | 293-832 | [»] | |
5O7T | X-ray | 1.80 | A | 293-832 | [»] | |
5OXJ | X-ray | 2.00 | A | 293-832 | [»] | |
5SZT | X-ray | 1.80 | A | 293-832 | [»] | |
5W6K | X-ray | 2.34 | A | 293-832 | [»] | |
5W6Q | X-ray | 2.66 | A/C/G | 293-832 | [»] | |
5YTC | X-ray | 2.28 | A | 294-832 | [»] | |
5YTD | X-ray | 2.00 | A | 294-832 | [»] | |
5YTE | X-ray | 2.21 | A | 294-832 | [»] | |
5YTF | X-ray | 1.98 | A | 294-832 | [»] | |
5YTG | X-ray | 2.07 | A | 294-832 | [»] | |
5YTH | X-ray | 2.53 | A | 294-832 | [»] | |
5Z3N | X-ray | 1.91 | A | 294-832 | [»] | |
6FBC | X-ray | 1.54 | A | 293-832 | [»] | |
6FBD | X-ray | 2.10 | A | 293-832 | [»] | |
6FBE | X-ray | 1.59 | A | 293-832 | [»] | |
6FBF | X-ray | 2.00 | A | 293-832 | [»] | |
6FBG | X-ray | 1.70 | A | 293-832 | [»] | |
6FBH | X-ray | 1.80 | A | 293-832 | [»] | |
6FBI | X-ray | 1.90 | A | 293-832 | [»] | |
6Q4U | X-ray | 2.00 | A | 293-832 | [»] | |
6Q4V | X-ray | 2.01 | A | 293-832 | [»] | |
SMRi | P19821 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
BindingDBi | P19821 |
ChEMBLi | CHEMBL3564 |
DrugBanki | DB03258, 2'-Deoxycytidine 5'-triphosphate DB03152, B-2-Octylglucoside |
Protocols and materials databases
ABCDi | P19821, 1 sequenced antibody |
Enzyme and pathway databases
BRENDAi | 2.7.7.7, 6334 |
Miscellaneous databases
EvolutionaryTracei | P19821 |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf IPR015361, Taq_pol_thermo_exonuc |
PANTHERi | PTHR10133, PTHR10133, 2 hits |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit PF09281, Taq-exonuc, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00475, 53EXOc, 1 hit SM00278, HhH1, 2 hits SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO1_THEAQ | |
Accessioni | P19821Primary (citable) accession number: P19821 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | September 29, 2021 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families