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UniProtKB - P19821 (DPO1_THEAQ)

Entry version 142 (29 Sep 2021)
Sequence version 1 (01 Feb 1991)
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Protein

DNA polymerase I, thermostable

Gene

polA

Organism
Thermus aquaticus
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.7.7, 6334

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA polymerase I, thermostable (EC:2.7.7.7)
Alternative name(s):
Taq polymerase 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:polA
Synonyms:pol1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiThermus aquaticus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri271 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the use of a specific protein in the biotechnological industry.<p><a href='/help/biotechnological_use' target='_top'>More...</a></p>Biotechnological usei

Used in the PCR method because of its high thermostability. Has a relatively high error rate because it lacks exonuclease proofreading functionality.

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL3564

Drug and drug target database

More...DrugBanki		DB03258, 2'-Deoxycytidine 5'-triphosphate
DB03152, B-2-Octylglucoside

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001012561 – 832DNA polymerase I, thermostableAdd BLAST832

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		P19821

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1832
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P19821

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P19821

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini175 – 2615'-3' exonucleaseSequence analysisAdd BLAST87

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni410 – 832PolymeraseBy similarityAdd BLAST423

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated

Family and domain databases

Conserved Domains Database

More...CDDi		cd09898, H3TH_53EXO, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.30.420.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR020046, 5-3_exonucl_a-hlix_arch_N
IPR002421, 5-3_exonuclease
IPR036279, 5-3_exonuclease_C_sf
IPR019760, DNA-dir_DNA_pol_A_CS
IPR001098, DNA-dir_DNA_pol_A_palm_dom
IPR043502, DNA/RNA_pol_sf
IPR020045, DNA_polI_H3TH
IPR018320, DNA_polymerase_1
IPR002298, DNA_polymerase_A
IPR008918, HhH2
IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif
IPR029060, PIN-like_dom_sf
IPR012337, RNaseH-like_sf
IPR036397, RNaseH_sf
IPR015361, Taq_pol_thermo_exonuc

The PANTHER Classification System

More...PANTHERi		PTHR10133, PTHR10133, 2 hits

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01367, 5_3_exonuc, 1 hit
PF02739, 5_3_exonuc_N, 1 hit
PF00476, DNA_pol_A, 1 hit
PF09281, Taq-exonuc, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00868, DNAPOLI

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00475, 53EXOc, 1 hit
SM00278, HhH1, 2 hits
SM00279, HhH2, 1 hit
SM00482, POLAc, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF47807, SSF47807, 1 hit
SSF53098, SSF53098, 1 hit
SSF56672, SSF56672, 1 hit
SSF88723, SSF88723, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00593, pola, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00447, DNA_POLYMERASE_A, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P19821-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRGMLPLFEP KGRVLLVDGH HLAYRTFHAL KGLTTSRGEP VQAVYGFAKS 
        60         70         80         90        100
LLKALKEDGD AVIVVFDAKA PSFRHEAYGG YKAGRAPTPE DFPRQLALIK 
       110        120        130        140        150
ELVDLLGLAR LEVPGYEADD VLASLAKKAE KEGYEVRILT ADKDLYQLLS 
       160        170        180        190        200
DRIHVLHPEG YLITPAWLWE KYGLRPDQWA DYRALTGDES DNLPGVKGIG 
       210        220        230        240        250
EKTARKLLEE WGSLEALLKN LDRLKPAIRE KILAHMDDLK LSWDLAKVRT 
       260        270        280        290        300
DLPLEVDFAK RREPDRERLR AFLERLEFGS LLHEFGLLES PKALEEAPWP 
       310        320        330        340        350
PPEGAFVGFV LSRKEPMWAD LLALAAARGG RVHRAPEPYK ALRDLKEARG 
       360        370        380        390        400
LLAKDLSVLA LREGLGLPPG DDPMLLAYLL DPSNTTPEGV ARRYGGEWTE 
       410        420        430        440        450
EAGERAALSE RLFANLWGRL EGEERLLWLY REVERPLSAV LAHMEATGVR 
       460        470        480        490        500
LDVAYLRALS LEVAEEIARL EAEVFRLAGH PFNLNSRDQL ERVLFDELGL 
       510        520        530        540        550
PAIGKTEKTG KRSTSAAVLE ALREAHPIVE KILQYRELTK LKSTYIDPLP 
       560        570        580        590        600
DLIHPRTGRL HTRFNQTATA TGRLSSSDPN LQNIPVRTPL GQRIRRAFIA 
       610        620        630        640        650
EEGWLLVALD YSQIELRVLA HLSGDENLIR VFQEGRDIHT ETASWMFGVP 
       660        670        680        690        700
REAVDPLMRR AAKTINFGVL YGMSAHRLSQ ELAIPYEEAQ AFIERYFQSF 
       710        720        730        740        750
PKVRAWIEKT LEEGRRRGYV ETLFGRRRYV PDLEARVKSV REAAERMAFN 
       760        770        780        790        800
MPVQGTAADL MKLAMVKLFP RLEEMGARML LQVHDELVLE APKERAEAVA 
       810        820        830 
RLAKEVMEGV YPLAVPLEVE VGIGEDWLSA KE
Length:832
Mass (Da):93,910
Last modified:February 1, 1991 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iF1731055B5246F03
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti155V → A in BAA06775 (PubMed:7896728).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
J04639 Genomic DNA Translation: AAA27507.1
D32013 Genomic DNA Translation: BAA06775.1

Protein sequence database of the Protein Information Resource

More...PIRi		A33530
JX0359

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04639 Genomic DNA Translation: AAA27507.1
D32013 Genomic DNA Translation: BAA06775.1
PIRiA33530
JX0359

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BGXX-ray2.30T1-832[»]
1JXEX-ray2.85A293-832[»]
1KTQX-ray2.50A290-832[»]
1QSSX-ray2.30A293-831[»]
1QSYX-ray2.30A293-831[»]
1QTMX-ray2.30A293-831[»]
1TAQX-ray2.40A1-832[»]
1TAUX-ray3.00A1-832[»]
2KTQX-ray2.30A295-832[»]
3KTQX-ray2.30A293-832[»]
3LWLX-ray2.25A293-832[»]
3LWMX-ray2.19A293-832[»]
3M8RX-ray2.00A293-832[»]
3M8SX-ray2.20A293-832[»]
3OJSX-ray1.90A293-832[»]
3OJUX-ray2.00A293-832[»]
3PO4X-ray1.80A293-832[»]
3PO5X-ray2.39A293-832[»]
3PY8X-ray1.74A293-832[»]
3RR7X-ray1.95A293-832[»]
3RR8X-ray2.40A293-832[»]
3RRGX-ray2.30A293-832[»]
3RRHX-ray1.80A293-832[»]
3RTVX-ray1.90A293-832[»]
3SV3X-ray2.10A293-832[»]
3SV4X-ray1.99A293-832[»]
3SYZX-ray1.95A293-832[»]
3SZ2X-ray2.15A293-832[»]
3T3FX-ray1.90A293-832[»]
4BWJX-ray1.55A293-832[»]
4BWMX-ray1.75A293-832[»]
4C8KX-ray2.17A293-832[»]
4C8LX-ray1.70A293-832[»]
4C8MX-ray1.57A293-832[»]
4C8NX-ray1.88A293-832[»]
4C8OX-ray1.75A293-832[»]
4CCHX-ray2.55A293-832[»]
4DF4X-ray2.20A293-832[»]
4DF8X-ray2.00A293-832[»]
4DFJX-ray1.90A293-832[»]
4DFKX-ray1.65A293-832[»]
4DFMX-ray1.89A293-832[»]
4DFPX-ray2.00A293-832[»]
4DLEX-ray2.44A293-832[»]
4DLGX-ray1.89A293-832[»]
4ELTX-ray2.20A293-832[»]
4ELUX-ray1.80A293-832[»]
4ELVX-ray1.90A293-832[»]
4KTQX-ray2.50A294-832[»]
4N56X-ray2.20A281-832[»]
4N5SX-ray1.67A281-832[»]
4XIUX-ray2.50A294-832[»]
5E41X-ray1.80A293-832[»]
5KTQX-ray2.50A290-832[»]
5NKLX-ray1.70A293-832[»]
5O7TX-ray1.80A293-832[»]
5OXJX-ray2.00A293-832[»]
5SZTX-ray1.80A293-832[»]
5W6KX-ray2.34A293-832[»]
5W6QX-ray2.66A/C/G293-832[»]
5YTCX-ray2.28A294-832[»]
5YTDX-ray2.00A294-832[»]
5YTEX-ray2.21A294-832[»]
5YTFX-ray1.98A294-832[»]
5YTGX-ray2.07A294-832[»]
5YTHX-ray2.53A294-832[»]
5Z3NX-ray1.91A294-832[»]
6FBCX-ray1.54A293-832[»]
6FBDX-ray2.10A293-832[»]
6FBEX-ray1.59A293-832[»]
6FBFX-ray2.00A293-832[»]
6FBGX-ray1.70A293-832[»]
6FBHX-ray1.80A293-832[»]
6FBIX-ray1.90A293-832[»]
6Q4UX-ray2.00A293-832[»]
6Q4VX-ray2.01A293-832[»]
SMRiP19821
ModBaseiSearch...
PDBe-KBiSearch...

Chemistry databases

BindingDBiP19821
ChEMBLiCHEMBL3564
DrugBankiDB03258, 2'-Deoxycytidine 5'-triphosphate
DB03152, B-2-Octylglucoside

Protocols and materials databases

ABCD curated depository of sequenced antibodies

More...ABCDi		P19821, 1 sequenced antibody

Enzyme and pathway databases

BRENDAi2.7.7.7, 6334

Miscellaneous databases

EvolutionaryTraceiP19821

Family and domain databases

CDDicd09898, H3TH_53EXO, 1 hit
Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR020046, 5-3_exonucl_a-hlix_arch_N
IPR002421, 5-3_exonuclease
IPR036279, 5-3_exonuclease_C_sf
IPR019760, DNA-dir_DNA_pol_A_CS
IPR001098, DNA-dir_DNA_pol_A_palm_dom
IPR043502, DNA/RNA_pol_sf
IPR020045, DNA_polI_H3TH
IPR018320, DNA_polymerase_1
IPR002298, DNA_polymerase_A
IPR008918, HhH2
IPR003583, Hlx-hairpin-Hlx_DNA-bd_motif
IPR029060, PIN-like_dom_sf
IPR012337, RNaseH-like_sf
IPR036397, RNaseH_sf
IPR015361, Taq_pol_thermo_exonuc
PANTHERiPTHR10133, PTHR10133, 2 hits
PfamiView protein in Pfam
PF01367, 5_3_exonuc, 1 hit
PF02739, 5_3_exonuc_N, 1 hit
PF00476, DNA_pol_A, 1 hit
PF09281, Taq-exonuc, 1 hit
PRINTSiPR00868, DNAPOLI
SMARTiView protein in SMART
SM00475, 53EXOc, 1 hit
SM00278, HhH1, 2 hits
SM00279, HhH2, 1 hit
SM00482, POLAc, 1 hit
SUPFAMiSSF47807, SSF47807, 1 hit
SSF53098, SSF53098, 1 hit
SSF56672, SSF56672, 1 hit
SSF88723, SSF88723, 1 hit
TIGRFAMsiTIGR00593, pola, 1 hit
PROSITEiView protein in PROSITE
PS00447, DNA_POLYMERASE_A, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDPO1_THEAQ
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P19821
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: September 29, 2021
This is version 142 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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