UniProtKB - P19811 (RPOA_EAVBU)
Replicase polyprotein 1ab
rep
Functioni
The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
1 PublicationNsp1 is essential for viral subgenomic mRNA synthesis.
1 PublicationNsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.
1 PublicationThe 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.
1 PublicationThe helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.
2 PublicationsPlays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR (By similarity).
Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) (PubMed:19297500).
If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors (By similarity).
By similarity1 PublicationMiscellaneous
Catalytic activityi
- Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). EC:3.4.19.12
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 164 | For Nsp1 papain-like cysteine proteinase activity2 Publications | 1 | |
Active sitei | 230 | For Nsp1 papain-like cysteine proteinase activity1 Publication | 1 | |
Active sitei | 270 | For Nsp2 cysteine proteinase activity1 Publication | 1 | |
Metal bindingi | 319 | Zinc 1Combined sources | 1 | |
Active sitei | 332 | For Nsp2 cysteine proteinase activity1 Publication | 1 | |
Metal bindingi | 349 | Zinc 1Combined sources | 1 | |
Metal bindingi | 354 | Zinc 1Combined sources | 1 | |
Metal bindingi | 356 | Zinc 1Combined sources | 1 | |
Active sitei | 1103 | Charge relay system; for 3C-like serine proteinase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1129 | Charge relay system; for 3C-like serine proteinase activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1184 | Charge relay system; for 3C-like serine proteinase activityPROSITE-ProRule annotation | 1 | |
Metal bindingi | 2374 | Zinc 2PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2377 | Zinc 2PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2387 | Zinc 3PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2392 | Zinc 2PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2395 | Zinc 2PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2399 | Zinc 3; via tele nitrogenPROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2402 | Zinc 3; via pros nitrogenPROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2403 | Zinc 3PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2412 | Zinc 4PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2414 | Zinc 4; via pros nitrogenPROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2423 | Zinc 4PROSITE-ProRule annotationCombined sources | 1 | |
Metal bindingi | 2426 | Zinc 4PROSITE-ProRule annotationCombined sources | 1 | |
Sitei | 2429 | Involved in mRNA transcription process | 1 | |
Active sitei | 2963 | PROSITE-ProRule annotation | 1 | |
Active sitei | 2978 | PROSITE-ProRule annotation | 1 | |
Active sitei | 3007 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 25 – 44 | C4-type; atypicalAdd BLAST | 20 | |
Nucleotide bindingi | 2528 – 2535 | ATPBy similarity | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- cysteine-type endopeptidase activity Source: InterPro
- DNA helicase activity Source: UniProtKB-EC
- endonuclease activity Source: UniProtKB-KW
- lyase activity Source: UniProtKB-KW
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- RNA helicase activity Source: UniProtKB-EC
- serine-type endopeptidase activity Source: InterPro
- serine-type exopeptidase activity Source: InterPro
- thiol-dependent deubiquitinase Source: UniProtKB-EC
- zinc ion binding Source: InterPro
GO - Biological processi
- modulation by virus of host protein ubiquitination Source: UniProtKB-KW
- suppression by virus of host ISG15-protein conjugation Source: UniProtKB-KW
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
- transcription, DNA-templated Source: InterPro
- viral protein processing Source: InterPro
- viral RNA genome replication Source: InterPro
Keywordsi
Enzyme and pathway databases
BRENDAi | 3.4.21.114, 6985 3.4.22.B50, 6985 |
Protein family/group databases
MEROPSi | S32.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Replicase polyprotein 1abAlternative name(s): ORF1ab polyprotein Cleaved into the following 14 chains: Nsp2 cysteine proteinase (EC:3.4.19.12, EC:3.4.22.-) Alternative name(s): CP2 Short name: CP Alternative name(s): Nsp4 Alternative name(s): Nsp9 Alternative name(s): Nsp10 Alternative name(s): Non-structural protein 11 Short name: Nsp11 |
Gene namesi | Name:rep ORF Names:1a-1b |
Organismi | Equine arteritis virus (strain Bucyrus) (EAV) |
Taxonomic identifieri | 299386 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Pisuviricota › Pisoniviricetes › Nidovirales › Arnidovirineae › Arteriviridae › Equarterivirinae › Alphaarterivirus › Alphaarterivirus equid › |
Virus hosti | Equidae (horses) [TaxID: 9788] |
Proteomesi |
|
Subcellular locationi
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- Host membrane Curated; Multi-pass membrane protein Curated
- host perinuclear region Curated
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 530 – 550 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 551 – 571 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 625 – 645 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 829 – 849 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 903 – 923 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 935 – 955 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 977 – 997 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1291 – 1311 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1333 – 1353 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1355 – 1375 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 1385 – 1405 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Host cytoplasm, Host membrane, Host nucleus, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 25 | C → A: Complete loss of transcription. 1 Publication | 1 | |
Mutagenesisi | 44 | C → A: Complete loss of transcription. 1 Publication | 1 | |
Mutagenesisi | 164 | C → G or S: Complete loss of PCP proteinase activity. 2 Publications | 1 | |
Mutagenesisi | 219 | H → A, G or V: No effect. 1 Publication | 1 | |
Mutagenesisi | 230 | H → A, G or V: Complete loss of PCP proteinase activity. 1 Publication | 1 | |
Mutagenesisi | 260 | G → V: Complete loss of nsp1-nsp2 cleavage. 1 Publication | 1 | |
Mutagenesisi | 270 | C → A, H, R or S: Complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 271 | G → W: Complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 291 | D → E or N: No effect. 1 Publication | 1 | |
Mutagenesisi | 295 | D → E or N: No effect. 1 Publication | 1 | |
Mutagenesisi | 296 | D → E or N: No effect. 1 Publication | 1 | |
Mutagenesisi | 297 | E → D or Q: No effect. 1 Publication | 1 | |
Mutagenesisi | 319 | C → A, H or P: Complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 332 | H → C, I, N or Y: Complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 344 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 344 | C → H: Almost complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 349 | C → A, H or S: Complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 354 | C → A, H or P: Complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 356 | C → A: Complete loss of CP2 activity. 1 Publication | 1 | |
Mutagenesisi | 356 | C → H: No effect. 1 Publication | 1 | |
Mutagenesisi | 831 | G → P: Complete loss of nsp2-nsp3 cleavage. 1 Publication | 1 | |
Mutagenesisi | 1064 | E → P: Complete loss of nsp3-nsp4 cleavage. 1 Publication | 1 | |
Mutagenesisi | 1103 | H → G or R: Complete loss of nsp3-nsp4, nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication | 1 | |
Mutagenesisi | 1117 | D → N or T: No effect. 1 Publication | 1 | |
Mutagenesisi | 1129 | D → E: Complete loss of nsp3-nsp4 and nsp5-nsp6 cleavages. 1 Publication | 1 | |
Mutagenesisi | 1129 | D → K or V: Complete loss of nsp3-nsp4, nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication | 1 | |
Mutagenesisi | 1179 | T → G or S: Partial loss of nsp4-nsp5 cleavage. 1 Publication | 1 | |
Mutagenesisi | 1179 | T → N: Increased nsp5-nsp6 cleavage. 1 Publication | 1 | |
Mutagenesisi | 1184 | S → C, F, I or T: Complete loss of nsp3-nsp4, nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication | 1 | |
Mutagenesisi | 1198 | H → L, R or Y: Complete loss of nsp4-nsp5 and nsp5-nsp6 cleavages. 1 Publication | 1 | |
Mutagenesisi | 1268 | E → P: Complete loss of nsp3-nsp4 and nsp4-nsp5 cleavages. 2 Publications | 1 | |
Mutagenesisi | 1430 | E → P: No effect. 1 Publication | 1 | |
Mutagenesisi | 1677 | E → P: Complete loss of nsp5-nsp6 cleavage. 1 Publication | 1 | |
Mutagenesisi | 2351 | D → P: No effect. 1 Publication | 1 | |
Mutagenesisi | 2370 | E → P: Complete loss of nsp9-nsp10 cleavage. 1 Publication | 1 | |
Mutagenesisi | 2429 | S → P: RNA can replicate efficiently but does not produce the subgenomic mRNAs required for structural protein expression. 1 Publication | 1 | |
Mutagenesisi | 2534 | K → Q: Abolishes ATPase and helicase activity. 1 Publication | 1 | |
Mutagenesisi | 2800 | E → P: No effect. 1 Publication | 1 | |
Mutagenesisi | 2819 | D → P: No effect. 1 Publication | 1 | |
Mutagenesisi | 2835 | E → D, P or Q: Almost complete loss of nsp9-nsp10 cleavage. 1 Publication | 1 | |
Mutagenesisi | 2837 | Q → D or N: No effect. 1 Publication | 1 | |
Mutagenesisi | 2837 | Q → E: Increased nsp9-nsp10 cleavage. 1 Publication | 1 | |
Mutagenesisi | 2837 | Q → P: Almost complete loss of nsp9-nsp10 cleavage. 1 Publication | 1 | |
Mutagenesisi | 3056 | E → P: Complete loss of nsp10-nsp11 cleavage. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000036619 | 1 – 3175 | Replicase polyprotein 1abAdd BLAST | 3175 | |
ChainiPRO_0000036621 | 1 – 260 | Nsp1 papain-like cysteine proteinaseAdd BLAST | 260 | |
ChainiPRO_0000036622 | 261 – 831 | Nsp2 cysteine proteinaseAdd BLAST | 571 | |
ChainiPRO_0000036623 | 832 – 1064 | Non-structural protein 3Add BLAST | 233 | |
ChainiPRO_0000036624 | 1065 – 1268 | 3C-like serine proteinaseAdd BLAST | 204 | |
ChainiPRO_0000036625 | 1269 – 1677 | Non-structural protein 5-6-7Add BLAST | 409 | |
ChainiPRO_0000423106 | 1269 – 1430 | Non-structural protein 5Add BLAST | 162 | |
ChainiPRO_0000423107 | 1431 – 1452 | Non-structural protein 6Add BLAST | 22 | |
ChainiPRO_0000423108 | 1453 – 1575 | Non-structural protein 7-alphaAdd BLAST | 123 | |
ChainiPRO_0000423109 | 1576 – 1677 | Non-structural protein 7-betaAdd BLAST | 102 | |
ChainiPRO_0000036626 | 1678 – 2370 | RNA-directed RNA polymeraseAdd BLAST | 693 | |
ChainiPRO_0000036627 | 1678 – 1727 | Non-structural protein 8Add BLAST | 50 | |
ChainiPRO_0000036628 | 2371 – 2837 | HelicaseAdd BLAST | 467 | |
ChainiPRO_0000036629 | 2838 – 3056 | Uridylate-specific endoribonuclease nsp11Add BLAST | 219 | |
ChainiPRO_0000036630 | 3057 – 3175 | Non-structural protein 12Add BLAST | 119 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 1501 | N-linked (GlcNAc...) asparagine; by hostCurated | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 260 – 261 | Cleavage; by PCP | 2 | |
Sitei | 831 – 832 | Cleavage; by Nsp2 cysteine proteinase | 2 | |
Sitei | 1064 – 1065 | Cleavage; by 3CLSP | 2 | |
Sitei | 1268 – 1269 | Cleavage; by 3CLSP; in major pathway | 2 | |
Sitei | 1430 – 1431 | Cleavage; by 3CLSP; in minor pathway | 2 | |
Sitei | 1452 – 1453 | Cleavage; by 3CLSP; in minor pathway | 2 | |
Sitei | 1575 – 1576 | Cleavage; by 3CLSP | 2 | |
Sitei | 1677 – 1678 | Cleavage; by 3CLSP | 2 | |
Sitei | 2370 – 2371 | Cleavage; by 3CLSP | 2 | |
Sitei | 2837 – 2838 | Cleavage; by 3CLSP | 2 | |
Sitei | 3056 – 3057 | Cleavage; by 3CLSP | 2 |
Keywords - PTMi
GlycoproteinProteomic databases
PRIDEi | P19811 |
Interactioni
Subunit structurei
Nsp1 interacts with cellular transcription cofactor SND1/p100.
1 PublicationBinary interactionsi
Structurei
Secondary structure
3D structure databases
BMRBi | P19811 |
SMRi | P19811 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P19811 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 66 – 156 | Peptidase C31PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 157 – 260 | Peptidase C32PROSITE-ProRule annotationAdd BLAST | 104 | |
Domaini | 261 – 360 | Peptidase C33PROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 1065 – 1268 | Peptidase S32PROSITE-ProRule annotationAdd BLAST | 204 | |
Domaini | 1716 – 1883 | NiRANPROSITE-ProRule annotationAdd BLAST | 168 | |
Domaini | 2116 – 2251 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 136 | |
Domaini | 2371 – 2438 | AV ZBDPROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 2496 – 2661 | (+)RNA virus helicase ATP-bindingAdd BLAST | 166 | |
Domaini | 2662 – 2793 | (+)RNA virus helicase C-terminalAdd BLAST | 132 | |
Domaini | 2840 – 2930 | AV-Nsp11N/CoV-Nsp15MPROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 2932 – 3054 | NendoUPROSITE-ProRule annotationAdd BLAST | 123 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 261 – 339 | OTU-likeAdd BLAST | 79 | |
Regioni | 386 – 451 | DisorderedSequence analysisAdd BLAST | 66 | |
Regioni | 530 – 645 | HD1Add BLAST | 116 | |
Regioni | 829 – 997 | HD2Add BLAST | 169 | |
Regioni | 1291 – 1405 | HD3Add BLAST | 115 | |
Regioni | 1577 – 1614 | DisorderedSequence analysisAdd BLAST | 38 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1593 – 1614 | Basic and acidic residuesSequence analysisAdd BLAST | 22 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 25 – 44 | C4-type; atypicalAdd BLAST | 20 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerFamily and domain databases
CDDi | cd21410, 1B_av_Nsp10-like, 1 hit cd21160, NendoU_av_Nsp11-like, 1 hit cd21166, NTD_av_Nsp11-like, 1 hit cd21405, ZBD_av_Nsp10-like, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 3.30.1330.220, 1 hit 3.30.40.20, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR031932, Arteri_nsp7a IPR038451, Arteri_nsp7a_sf IPR008743, Arterivirus_Nsp2_C33 IPR023338, Arterivirus_NSP4_peptidase IPR008741, AV_PCPalpha IPR025773, AV_PCPbeta IPR023183, Chymotrypsin-like_C IPR043502, DNA/RNA_pol_sf IPR022230, DUF3756 IPR029323, EAV_nsp1 IPR008760, EAV_peptidase_S32 IPR037227, EndoU-like IPR043609, NendoU_nidovirus IPR044863, NIRAN IPR044348, NSP10_1B_Av IPR027355, NSP10_Av_ZBD IPR044320, NSP11_Av_N IPR044314, NSP11_NendoU_Av IPR032786, NSP2_TM_arteriviridae IPR027417, P-loop_NTPase IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR041053, Rep_1B IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR041361, Znf-RING_13 |
Pfami | View protein in Pfam PF16749, Arteri_nsp7a, 1 hit PF19215, CoV_NSP15_C, 1 hit PF12581, DUF3756, 1 hit PF14754, IFR3_antag, 1 hit PF14755, Nsp2_AV, 1 hit PF05412, Peptidase_C33, 1 hit PF05579, Peptidase_S32, 1 hit PF00680, RdRP_1, 1 hit PF17873, Rep_1B, 1 hit PF01443, Viral_helicase1, 1 hit PF17977, zf-RING_13, 1 hit |
SUPFAMi | SSF142877, SSF142877, 1 hit SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51538, AV_CP, 1 hit PS51961, AV_NSP11N_COV_NSP15M, 1 hit PS51493, AV_NSP4_PRO, 1 hit PS51539, AV_PCP_ALPHA, 1 hit PS51540, AV_PCP_BETA, 1 hit PS51652, AV_ZBD, 1 hit PS51958, NENDOU, 1 hit PS51947, NIRAN, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsiribosomal frameshifting. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATFSATGFG GSFVRDWSLD LPDACEHGAG LCCEVDGSTL CAECFRGCEG
60 70 80 90 100
MEQCPGLFMG LLKLASPVPV GHKFLIGWYR AAKVTGRYNF LELLQHPAFA
110 120 130 140 150
QLRVVDARLA IEEASVFIST DHASAKRFPG ARFALTPVYA NAWVVSPAAN
160 170 180 190 200
SLIVTTDQEQ DGFCWLKLLP PDRREAGLRL YYNHYREQRT GWLSKTGLRL
210 220 230 240 250
WLGDLGLGIN ASSGGLKFHI MRGSPQRAWH ITTRSCKLKS YYVCDISEAD
260 270 280 290 300
WSCLPAGNYG GYNPPGDGAC GYRCLAFMNG ATVVSAGCSS DLWCDDELAY
310 320 330 340 350
RVFQLSPTFT VTIPGGRVCP NAKYAMICDK QHWRVKRAKG VGLCLDESCF
360 370 380 390 400
RGICNCQRMS GPPPAPVSAA VLDHILEAAT FGNVRVVTPE GQPRPVPAPR
410 420 430 440 450
VRPSANSSGD VKDPAPVPPV PKPRTKLATP NPTQAPIPAP RTRLQGASTQ
460 470 480 490 500
EPLASAGVAS DSAPKWRVAK TVYSSAERFR TELVQRARSV GDVLVQALPL
510 520 530 540 550
KTPAVQRYTM TLKMMRSRFS WHCDVWYPLA VIACLLPIWP SLALLLSFAI
560 570 580 590 600
GLIPSVGNNV VLTALLVSSA NYVASMDHQC EGAACLALLE EEHYYRAVRW
610 620 630 640 650
RPITGALSLV LNLLGQVGYV ARSTFDAAYV PCTVFDLCSF AILYLCRNRC
660 670 680 690 700
WRCFGRCVRV GPATHVLGST GQRVSKLALI DLCDHFSKPT IDVVGMATGW
710 720 730 740 750
SGCYTGTAAM ERQCASTVDP HSFDQKKAGA TVYLTPPVNS GSALQCLNVM
760 770 780 790 800
WKRPIGSTVL GEQTGAVVTA VKSISFSPPC CVSTTLPTRP GVTVVDHALY
810 820 830 840 850
NRLTASGVDP ALLRVGQGDF LKLNPGFRLI GGWIYGICYF VLVVVSTFTC
860 870 880 890 900
LPIKCGIGTR DPFCRRVFSV PVTKTQEHCH AGMCASAEGI SLDSLGLTQL
910 920 930 940 950
QSYWIAAVTS GLVILLVCHR LAISALDLLT LASPLVLLVF PWASVGLLLA
960 970 980 990 1000
CSLAGAAVKI QLLATLFVNL FFPQATLVTM GYWACVAALA VYSLMGLRVK
1010 1020 1030 1040 1050
VNVPMCVTPA HFLLLARSAG QSREQMLRVS AAAPTNSLLG VARDCYVTGT
1060 1070 1080 1090 1100
TRLYIPKEGG MVFEGLFRSP KARGNVGFVA GSSYGTGSVW TRNNEVVVLT
1110 1120 1130 1140 1150
ASHVVGRANM ATLKIGDAML TLTFKKNGDF AEAVTTQSEL PGNWPQLHFA
1160 1170 1180 1190 1200
QPTTGPASWC TATGDEEGLL SGEVCLAWTT SGDSGSAVVQ GDAVVGVHTG
1210 1220 1230 1240 1250
SNTSGVAYVT TPSGKLLGAD TVTLSSLSKH FTGPLTSIPK DIPDNIIADV
1260 1270 1280 1290 1300
DAVPRSLAML IDGLSNRESS LSGPQLLLIA CFMWSYLNQP AYLPYVLGFF
1310 1320 1330 1340 1350
AANFFLPKSV GRPVVTGLLW LCCLFTPLSM RLCLFHLVCA TVTGNVISLW
1360 1370 1380 1390 1400
FYITAAGTSY LSEMWFGGYP TMLFVPRFLV YQFPGWAIGT VLAVCSITML
1410 1420 1430 1440 1450
AAALGHTLLL DVFSASGRFD RTFMMKYFLE GGVKESVTAS VTRAYGKPIT
1460 1470 1480 1490 1500
QESLTATLAA LTDDDFQFLS DVLDCRAVRS AMNLRAALTS FQVAQYRNIL
1510 1520 1530 1540 1550
NASLQVDRDA ARSRRLMAKL ADFAVEQEVT AGDRVVVIDG LDRMAHFKDD
1560 1570 1580 1590 1600
LVLVPLTTKV VGGSRCTICD VVKEEANDTP VKPMPSRRRR KGLPKGAQLE
1610 1620 1630 1640 1650
WDRHQEEKRN AGDDDFAVSN DYVKRVPKYW DPSDTRGTTV KIAGTTYQKV
1660 1670 1680 1690 1700
VDYSGNVHYV EHQEDLLDYV LGKGSYEGLD QDKVLDLTNM LKVDPTELSS
1710 1720 1730 1740 1750
KDKAKARQLA HLLLDLANPV EAVNQLNLRA PHIFPGDVGR RTFADSKDKG
1760 1770 1780 1790 1800
FVALHSRTMF LAARDFLFNI KFVCDEEFTK TPKDTLLGYV RACPGYWFIF
1810 1820 1830 1840 1850
RRTHRSLIDA YWDSMECVYA LPTISDFDVS PGDVAVTGER WDFESPGGGR
1860 1870 1880 1890 1900
AKRLTADLVH AFQGFHGASY SYDDKVAAAV SGDPYRSDGV LYNTRWGNIP
1910 1920 1930 1940 1950
YSVPTNALEA TACYRAGCEA VTDGTNVIAT IGPFPEQQPI PDIPKSVLDN
1960 1970 1980 1990 2000
CADISCDAFI APAAETALCG DLEKYNLSTQ GFVLPSVFSM VRAYLKEEIG
2010 2020 2030 2040 2050
DAPPLYLPST VPSKNSQAGI NGAEFPTKSL QSYCLIDDMV SQSMKSNLQT
2060 2070 2080 2090 2100
ATMATCKRQY CSKYKIRSIL GTNNYIGLGL RACLSGVTAA FQKAGKDGSP
2110 2120 2130 2140 2150
IYLGKSKFDP IPAPDKYCLE TDLESCDRST PALVRWFATN LIFELAGQPE
2160 2170 2180 2190 2200
LVHSYVLNCC HDLVVAGSVA FTKRGGLSSG DPITSISNTI YSLVLYTQHM
2210 2220 2230 2240 2250
LLCGLEGYFP EIAEKYLDGS LELRDMFKYV RVYIYSDDVV LTTPNQHYAA
2260 2270 2280 2290 2300
SFDRWVPHLQ ALLGFKVDPK KTVNTSSPSF LGCRFKQVDG KCYLASLQDR
2310 2320 2330 2340 2350
VTRSLLYHIG AKNPSEYYEA AVSIFKDSII CCDEDWWTDL HRRISGAART
2360 2370 2380 2390 2400
DGVEFPTIEM LTSFRTKQYE SAVCTVCGAA PVAKSACGGW FCGNCVPYHA
2410 2420 2430 2440 2450
GHCHTTSLFA NCGHDIMYRS TYCTMCEGSP KQMVPKVPHP ILDHLLCHID
2460 2470 2480 2490 2500
YGSKEELTLV VADGRTTSPP GRYKVGHKVV AVVADVGGNI VFGCGPGSHI
2510 2520 2530 2540 2550
AVPLQDTLKG VVVNKALKNA AASEYVEGPP GSGKTFHLVK DVLAVVGSAT
2560 2570 2580 2590 2600
LVVPTHASML DCINKLKQAG ADPYFVVPKY TVLDFPRPGS GNITVRLPQV
2610 2620 2630 2640 2650
GTSEGETFVD EVAYFSPVDL ARILTQGRVK GYGDLNQLGC VGPASVPRNL
2660 2670 2680 2690 2700
WLRHFVSLEP LRVCHRFGAA VCDLIKGIYP YYEPAPHTTK VVFVPNPDFE
2710 2720 2730 2740 2750
KGVVITAYHK DRGLGHRTID SIQGCTFPVV TLRLPTPQSL TRPRAVVAVT
2760 2770 2780 2790 2800
RASQELYIYD PFDQLSGLLK FTKEAEAQDL IHGPPTACHL GQEIDLWSNE
2810 2820 2830 2840 2850
GLEYYKEVNL LYTHVPIKDG VIHSYPNCGP ACGWEKQSNK ISCLPRVAQN
2860 2870 2880 2890 2900
LGYHYSPDLP GFCPIPKELA EHWPVVSNDR YPNCLQITLQ QVCELSKPCS
2910 2920 2930 2940 2950
AGYMVGQSVF VQTPGVTSYW LTEWVDGKAR ALPDSLFSSG RFETNSRAFL
2960 2970 2980 2990 3000
DEAEEKFAAA HPHACLGEIN KSTVGGSHFI FSQYLPPLLP ADAVALVGAS
3010 3020 3030 3040 3050
LAGKAAKAAC SVVDVYAPSF EPYLHPETLS RVYKIMIDFK PCRLMVWRNA
3060 3070 3080 3090 3100
TFYVQEGVDA VTSALAAVSK LIKVPANEPV SFHVASGYRT NALVAPQAKI
3110 3120 3130 3140 3150
SIGAYAAEWA LSTEPPPAGY AIVRRYIVKR LLSSTEVFLC RRGVVSSTSV
3160 3170
QTICALEGCK PLFNFLQIGS VIGPV
The sequence of this isoform differs from the canonical sequence as follows:
1728-3175: Missing.
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_032887 | 1728 – 3175 | Missing in isoform Replicase polyprotein 1a. CuratedAdd BLAST | 1448 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53459 Genomic RNA Translation: CAC42774.2 X53459 Genomic RNA Translation: CAC42775.2 X52277 Genomic RNA Translation: CAA36520.1 |
PIRi | A39925, RRWVEV |
RefSeqi | NP_127506.1, NC_002532.2 NP_127507.1, NC_002532.2 |
Genome annotation databases
GeneIDi | 921339 |
KEGGi | vg:921339 |
Keywords - Coding sequence diversityi
Ribosomal frameshiftingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53459 Genomic RNA Translation: CAC42774.2 X53459 Genomic RNA Translation: CAC42775.2 X52277 Genomic RNA Translation: CAA36520.1 |
PIRi | A39925, RRWVEV |
RefSeqi | NP_127506.1, NC_002532.2 NP_127507.1, NC_002532.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MBM | X-ray | 2.00 | A/B/C/D | 1071-1268 | [»] | |
2L8K | NMR | - | A | 1454-1575 | [»] | |
4IUM | X-ray | 1.45 | A | 261-392 | [»] | |
4N0N | X-ray | 2.00 | A | 2371-2772 | [»] | |
4N0O | X-ray | 2.65 | A/C/E/G | 2371-2772 | [»] | |
5F17 | X-ray | 3.20 | A/B/C/D/E/F | 2838-3056 | [»] | |
5HBZ | X-ray | 3.10 | A/B/C/D/E/F | 2838-3056 | [»] | |
5HC1 | X-ray | 3.10 | A/B/C/D | 2838-3056 | [»] | |
BMRBi | P19811 | |||||
SMRi | P19811 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
MEROPSi | S32.001 |
Proteomic databases
PRIDEi | P19811 |
Genome annotation databases
GeneIDi | 921339 |
KEGGi | vg:921339 |
Enzyme and pathway databases
BRENDAi | 3.4.21.114, 6985 3.4.22.B50, 6985 |
Miscellaneous databases
EvolutionaryTracei | P19811 |
Family and domain databases
CDDi | cd21410, 1B_av_Nsp10-like, 1 hit cd21160, NendoU_av_Nsp11-like, 1 hit cd21166, NTD_av_Nsp11-like, 1 hit cd21405, ZBD_av_Nsp10-like, 1 hit |
Gene3Di | 2.40.10.10, 2 hits 3.30.1330.220, 1 hit 3.30.40.20, 1 hit 3.30.70.270, 1 hit |
InterProi | View protein in InterPro IPR027351, (+)RNA_virus_helicase_core_dom IPR031932, Arteri_nsp7a IPR038451, Arteri_nsp7a_sf IPR008743, Arterivirus_Nsp2_C33 IPR023338, Arterivirus_NSP4_peptidase IPR008741, AV_PCPalpha IPR025773, AV_PCPbeta IPR023183, Chymotrypsin-like_C IPR043502, DNA/RNA_pol_sf IPR022230, DUF3756 IPR029323, EAV_nsp1 IPR008760, EAV_peptidase_S32 IPR037227, EndoU-like IPR043609, NendoU_nidovirus IPR044863, NIRAN IPR044348, NSP10_1B_Av IPR027355, NSP10_Av_ZBD IPR044320, NSP11_Av_N IPR044314, NSP11_NendoU_Av IPR032786, NSP2_TM_arteriviridae IPR027417, P-loop_NTPase IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR041053, Rep_1B IPR043128, Rev_trsase/Diguanyl_cyclase IPR001205, RNA-dir_pol_C IPR007094, RNA-dir_pol_PSvirus IPR041361, Znf-RING_13 |
Pfami | View protein in Pfam PF16749, Arteri_nsp7a, 1 hit PF19215, CoV_NSP15_C, 1 hit PF12581, DUF3756, 1 hit PF14754, IFR3_antag, 1 hit PF14755, Nsp2_AV, 1 hit PF05412, Peptidase_C33, 1 hit PF05579, Peptidase_S32, 1 hit PF00680, RdRP_1, 1 hit PF17873, Rep_1B, 1 hit PF01443, Viral_helicase1, 1 hit PF17977, zf-RING_13, 1 hit |
SUPFAMi | SSF142877, SSF142877, 1 hit SSF50494, SSF50494, 1 hit SSF52540, SSF52540, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51538, AV_CP, 1 hit PS51961, AV_NSP11N_COV_NSP15M, 1 hit PS51493, AV_NSP4_PRO, 1 hit PS51539, AV_PCP_ALPHA, 1 hit PS51540, AV_PCP_BETA, 1 hit PS51652, AV_ZBD, 1 hit PS51958, NENDOU, 1 hit PS51947, NIRAN, 1 hit PS51657, PSRV_HELICASE, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RPOA_EAVBU | |
Accessioni | P19811Primary (citable) accession number: P19811 Secondary accession number(s): Q88625, Q8QZQ5, Q91DM2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | April 13, 2004 | |
Last modified: | February 23, 2022 | |
This is version 176 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families