UniProtKB - P19804 (NDKB_RAT)
Protein
Nucleoside diphosphate kinase B
Gene
Nme2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate (By similarity). Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically. Binds to both single-stranded guanine- and cytosine-rich strands within the nuclease hypersensitive element (NHE) III1 region of the MYC gene promoter. Does not bind to duplex NHE III1. Has G-quadruplex (G4) DNA-binding activity, which is independent of its nucleotide-binding and kinase activity. Binds both folded and unfolded G4 with similar low nanomolar affinities. Stabilizes folded G4s regardless of whether they are prefolded or not. Exhibits histidine protein kinase activity (By similarity).By similarity
Catalytic activityi
- a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADPBy similarityEC:2.7.4.6By similarity
- EC:2.7.4.6By similarity
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.By similarity EC:2.7.13.3
Cofactori
Mg2+By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | ATPBy similarity | 1 | |
Binding sitei | 60 | ATPBy similarity | 1 | |
Binding sitei | 88 | ATPBy similarity | 1 | |
Binding sitei | 94 | ATPBy similarity | 1 | |
Binding sitei | 105 | ATPBy similarity | 1 | |
Binding sitei | 115 | ATPBy similarity | 1 | |
Active sitei | 118 | Pros-phosphohistidine intermediateBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB
- drug binding Source: RGD
- enzyme binding Source: RGD
- fatty acid binding Source: RGD
- GDP binding Source: RGD
- G-quadruplex DNA binding Source: UniProtKB
- identical protein binding Source: RGD
- intermediate filament binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- nucleoside diphosphate kinase activity Source: RGD
- protein histidine kinase activity Source: UniProtKB-EC
- protein serine/threonine kinase activity Source: RGD
- transcription coactivator activity Source: RGD
GO - Biological processi
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: RGD
- cellular response to fatty acid Source: RGD
- cellular response to glucose stimulus Source: RGD
- cellular response to oxidative stress Source: RGD
- CTP biosynthetic process Source: RGD
- GTP biosynthetic process Source: RGD
- integrin-mediated signaling pathway Source: UniProtKB
- negative regulation of apoptotic process Source: HGNC-UCL
- negative regulation of myeloid leukocyte differentiation Source: RGD
- nucleoside diphosphate phosphorylation Source: RGD
- nucleoside triphosphate biosynthetic process Source: RGD
- positive regulation of epithelial cell proliferation Source: HGNC-UCL
- positive regulation of keratinocyte differentiation Source: HGNC-UCL
- positive regulation of neuron projection development Source: RGD
- positive regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: UniProtKB
- protein autophosphorylation Source: RGD
- regulation of apoptotic process Source: GO_Central
- regulation of epidermis development Source: HGNC-UCL
- response to growth hormone Source: RGD
- UTP biosynthetic process Source: RGD
Keywordsi
Molecular function | DNA-binding, Kinase, Transferase |
Biological process | Nucleotide metabolism, Transcription, Transcription regulation |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-499943, Interconversion of nucleotide di- and triphosphates R-RNO-6798695, Neutrophil degranulation |
SABIO-RKi | P19804 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Nme2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619877, Nme2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm By similarity
- lamellipodium By similarity
- ruffle By similarity
Note: Colocalizes with ITGB1 and ITGB1BP1 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen but not on vitronectin or laminin substrates.By similarity
Mitochondrion
- mitochondrial membrane Source: RGD
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- cell periphery Source: UniProtKB
- cytoplasm Source: UniProtKB
- lamellipodium Source: HGNC-UCL
- perinuclear region of cytoplasm Source: RGD
- ruffle Source: HGNC-UCL
Keywords - Cellular componenti
Cell projection, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000137119 | 1 – 152 | Nucleoside diphosphate kinase BAdd BLAST | 152 |
Post-translational modificationi
The N-terminus is blocked.
Proteomic databases
jPOSTi | P19804 |
PaxDbi | P19804 |
PRIDEi | P19804 |
2D gel databases
World-2DPAGEi | 0004:P19804 |
PTM databases
iPTMneti | P19804 |
PhosphoSitePlusi | P19804 |
SwissPalmi | P19804 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000002671, Expressed in heart and 21 other tissues |
Genevisiblei | P19804, RN |
Interactioni
Subunit structurei
Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6) (By similarity).
Interacts with CAPN8 (By similarity).
Interacts with AKAP13. Interacts ITGB1BP1 (via C-terminal domain region) (By similarity).
By similarityGO - Molecular functioni
- enzyme binding Source: RGD
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249827, 4 interactors |
IntActi | P19804, 1 interactor |
MINTi | P19804 |
STRINGi | 10116.ENSRNOP00000003611 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 66 | Interaction with AKAP13By similarityAdd BLAST | 66 |
Sequence similaritiesi
Belongs to the NDK family.Curated
Phylogenomic databases
eggNOGi | KOG0888, Eukaryota |
GeneTreei | ENSGT00940000161569 |
HOGENOMi | CLU_060216_6_3_1 |
InParanoidi | P19804 |
OMAi | GLWFKKE |
OrthoDBi | 1334716at2759 |
PhylomeDBi | P19804 |
TreeFami | TF106373 |
Family and domain databases
Gene3Di | 3.30.70.141, 1 hit |
HAMAPi | MF_00451, NDP_kinase, 1 hit |
InterProi | View protein in InterPro IPR034907, NDK-like_dom IPR036850, NDK-like_dom_sf IPR001564, Nucleoside_diP_kinase IPR023005, Nucleoside_diP_kinase_AS |
Pfami | View protein in Pfam PF00334, NDK, 1 hit |
PRINTSi | PR01243, NUCDPKINASE |
SMARTi | View protein in SMART SM00562, NDK, 1 hit |
SUPFAMi | SSF54919, SSF54919, 1 hit |
PROSITEi | View protein in PROSITE PS00469, NDP_KINASES, 1 hit |
i Sequence
Sequence statusi: Complete.
P19804-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ
60 70 80 90 100
HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK
110 120 130 140 150
PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFKPEELI DYKSCAHDWV
YE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | V → W in AAA42017 (PubMed:1316151).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55331 mRNA Translation: AAA41684.1 M91597 mRNA Translation: AAA42017.1 BC086599 mRNA Translation: AAH86599.1 |
PIRi | A41849, A38369 |
RefSeqi | NP_114021.2, NM_031833.2 |
Genome annotation databases
Ensembli | ENSRNOT00000003611; ENSRNOP00000003611; ENSRNOG00000002671 |
GeneIDi | 83782 |
KEGGi | rno:83782 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M55331 mRNA Translation: AAA41684.1 M91597 mRNA Translation: AAA42017.1 BC086599 mRNA Translation: AAH86599.1 |
PIRi | A41849, A38369 |
RefSeqi | NP_114021.2, NM_031833.2 |
3D structure databases
SMRi | P19804 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249827, 4 interactors |
IntActi | P19804, 1 interactor |
MINTi | P19804 |
STRINGi | 10116.ENSRNOP00000003611 |
PTM databases
iPTMneti | P19804 |
PhosphoSitePlusi | P19804 |
SwissPalmi | P19804 |
2D gel databases
World-2DPAGEi | 0004:P19804 |
Proteomic databases
jPOSTi | P19804 |
PaxDbi | P19804 |
PRIDEi | P19804 |
Genome annotation databases
Ensembli | ENSRNOT00000003611; ENSRNOP00000003611; ENSRNOG00000002671 |
GeneIDi | 83782 |
KEGGi | rno:83782 |
Organism-specific databases
CTDi | 4831 |
RGDi | 619877, Nme2 |
Phylogenomic databases
eggNOGi | KOG0888, Eukaryota |
GeneTreei | ENSGT00940000161569 |
HOGENOMi | CLU_060216_6_3_1 |
InParanoidi | P19804 |
OMAi | GLWFKKE |
OrthoDBi | 1334716at2759 |
PhylomeDBi | P19804 |
TreeFami | TF106373 |
Enzyme and pathway databases
Reactomei | R-RNO-499943, Interconversion of nucleotide di- and triphosphates R-RNO-6798695, Neutrophil degranulation |
SABIO-RKi | P19804 |
Miscellaneous databases
PROi | PR:P19804 |
Gene expression databases
Bgeei | ENSRNOG00000002671, Expressed in heart and 21 other tissues |
Genevisiblei | P19804, RN |
Family and domain databases
Gene3Di | 3.30.70.141, 1 hit |
HAMAPi | MF_00451, NDP_kinase, 1 hit |
InterProi | View protein in InterPro IPR034907, NDK-like_dom IPR036850, NDK-like_dom_sf IPR001564, Nucleoside_diP_kinase IPR023005, Nucleoside_diP_kinase_AS |
Pfami | View protein in Pfam PF00334, NDK, 1 hit |
PRINTSi | PR01243, NUCDPKINASE |
SMARTi | View protein in SMART SM00562, NDK, 1 hit |
SUPFAMi | SSF54919, SSF54919, 1 hit |
PROSITEi | View protein in PROSITE PS00469, NDP_KINASES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NDKB_RAT | |
Accessioni | P19804Primary (citable) accession number: P19804 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 184 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families