UniProtKB - P19786 (AROA_SALTI)
Protein
3-phosphoshikimate 1-carboxyvinyltransferase
Gene
aroA
Organism
Salmonella typhi
Status
Functioni
Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation
Catalytic activityi
- 3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphateUniRule annotationEC:2.5.1.19UniRule annotation
: chorismate biosynthesis Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotationProteins known to be involved in the 7 steps of the subpathway in this organism are:
- Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG)
- 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB), 3-dehydroquinate synthase (aroB)
- 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (aroD), 3-dehydroquinate dehydratase (aroD)
- Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
- Shikimate kinase 2 (aroL), Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK), Shikimate kinase 1 (aroK)
- 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA), 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
- Chorismate synthase (aroC), Chorismate synthase (aroC), Chorismate synthase (aroC), Chorismate synthase (aroC)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 27 | Shikimate-3-phosphateUniRule annotation | 1 | |
Binding sitei | 124 | PhosphoenolpyruvateUniRule annotation | 1 | |
Binding sitei | 197 | Shikimate-3-phosphateUniRule annotation | 1 | |
Active sitei | 313 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 336 | Shikimate-3-phosphateUniRule annotation | 1 | |
Binding sitei | 340 | Shikimate-3-phosphateUniRule annotation | 1 | |
Active sitei | 341 | Proton donorUniRule annotation | 1 | |
Binding sitei | 344 | PhosphoenolpyruvateUniRule annotation | 1 | |
Binding sitei | 386 | PhosphoenolpyruvateUniRule annotation | 1 | |
Binding sitei | 411 | PhosphoenolpyruvateUniRule annotation | 1 |
GO - Molecular functioni
- 3-phosphoshikimate 1-carboxyvinyltransferase activity Source: UniProtKB-UniRule
GO - Biological processi
- aromatic amino acid family biosynthetic process Source: UniProtKB-UniRule
- chorismate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis |
Enzyme and pathway databases
UniPathwayi | UPA00053;UER00089 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)Alternative name(s): 5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation Short name: EPSP synthaseUniRule annotation Short name: EPSPSUniRule annotation |
Gene namesi | Name:aroAUniRule annotation Ordered Locus Names:STY0978, t1956 |
Organismi | Salmonella typhi |
Taxonomic identifieri | 90370 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Salmonella › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088285 | 1 – 427 | 3-phosphoshikimate 1-carboxyvinyltransferaseAdd BLAST | 427 |
Interactioni
Subunit structurei
Monomer.
UniRule annotationProtein-protein interaction databases
STRINGi | 220341.16502141 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 23 | Shikimate-3-phosphate bindingUniRule annotation | 2 | |
Regioni | 94 – 97 | PhosphoenolpyruvateUniRule annotation | 4 | |
Regioni | 169 – 171 | Shikimate-3-phosphate bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0128, Bacteria |
HOGENOMi | CLU_024321_0_0_6 |
Family and domain databases
Gene3Di | 3.65.10.10, 2 hits |
HAMAPi | MF_00210, EPSP_synth, 1 hit |
InterProi | View protein in InterPro IPR001986, Enolpyruvate_Tfrase_dom IPR036968, Enolpyruvate_Tfrase_sf IPR006264, EPSP_synthase IPR023193, EPSP_synthase_CS IPR013792, RNA3'P_cycl/enolpyr_Trfase_a/b |
Pfami | View protein in Pfam PF00275, EPSP_synthase, 1 hit |
PIRSFi | PIRSF000505, EPSPS, 1 hit |
SUPFAMi | SSF55205, SSF55205, 1 hit |
TIGRFAMsi | TIGR01356, aroA, 1 hit |
PROSITEi | View protein in PROSITE PS00104, EPSP_SYNTHASE_1, 1 hit PS00885, EPSP_SYNTHASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P19786-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESLTLQPIA RVDGAINLPG SKSVSNRALL LAALACGKTV LTNLLDSDDV
60 70 80 90 100
RHMLNALSAL GINYTLSADR TRCDITGNGG PLRASGTLEL FLGNAGTAMR
110 120 130 140 150
PLAAALCLGQ NEIVLTGEPR MKERPIGHLV DSLRQGGANI DYLEQENYPP
160 170 180 190 200
LRLRGGFIGG DIEVDGSVSS QFLTALLMTA PLAPEDTIIR VKGELVSKPY
210 220 230 240 250
IDITLNLMKT FGVEIANHHY QQFVVKGGQQ YHSPGRYLVE GDASSASYFL
260 270 280 290 300
AAGAIKGGTV KVTGIGRKSM QGDIRFADVL EKMGATITWG DDFIACTRGE
310 320 330 340 350
LHAIDMDMNH IPDAAMTIAT TALFAKGTTT LRNIYNWRVK ETDRLFAMAT
360 370 380 390 400
ELRKVGAEVE EGHDYIRITP PAKLQHADIG TYNDHRMAMC FSLVALSDTP
410 420
VTILDPKCTA KTFPDYFEQL ARMSTPA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 254 | A → G in CAA38417 (PubMed:2235501).Curated | 1 | |
Sequence conflicti | 267 | R → G in CAA38417 (PubMed:2235501).Curated | 1 | |
Sequence conflicti | 281 | E → H in CAA38417 (PubMed:2235501).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54545 Genomic DNA Translation: CAA38417.1 AL513382 Genomic DNA Translation: CAD05378.1 AE014613 Genomic DNA Translation: AAO69570.1 |
PIRi | S12096 |
RefSeqi | NP_455465.1, NC_003198.1 WP_000445205.1, NZ_WSUR01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAO69570; AAO69570; t1956 CAD05378; CAD05378; CAD05378 |
KEGGi | stt:t1956 sty:STY0978 |
PATRICi | fig|220341.7.peg.987 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54545 Genomic DNA Translation: CAA38417.1 AL513382 Genomic DNA Translation: CAD05378.1 AE014613 Genomic DNA Translation: AAO69570.1 |
PIRi | S12096 |
RefSeqi | NP_455465.1, NC_003198.1 WP_000445205.1, NZ_WSUR01000013.1 |
3D structure databases
SMRi | P19786 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 220341.16502141 |
Genome annotation databases
EnsemblBacteriai | AAO69570; AAO69570; t1956 CAD05378; CAD05378; CAD05378 |
KEGGi | stt:t1956 sty:STY0978 |
PATRICi | fig|220341.7.peg.987 |
Phylogenomic databases
eggNOGi | COG0128, Bacteria |
HOGENOMi | CLU_024321_0_0_6 |
Enzyme and pathway databases
UniPathwayi | UPA00053;UER00089 |
Family and domain databases
Gene3Di | 3.65.10.10, 2 hits |
HAMAPi | MF_00210, EPSP_synth, 1 hit |
InterProi | View protein in InterPro IPR001986, Enolpyruvate_Tfrase_dom IPR036968, Enolpyruvate_Tfrase_sf IPR006264, EPSP_synthase IPR023193, EPSP_synthase_CS IPR013792, RNA3'P_cycl/enolpyr_Trfase_a/b |
Pfami | View protein in Pfam PF00275, EPSP_synthase, 1 hit |
PIRSFi | PIRSF000505, EPSPS, 1 hit |
SUPFAMi | SSF55205, SSF55205, 1 hit |
TIGRFAMsi | TIGR01356, aroA, 1 hit |
PROSITEi | View protein in PROSITE PS00104, EPSP_SYNTHASE_1, 1 hit PS00885, EPSP_SYNTHASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AROA_SALTI | |
Accessioni | P19786Primary (citable) accession number: P19786 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | December 5, 2001 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families