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Protein

Casein kinase II subunit alpha'

Gene

CSNK2A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV.3 Publications

Miscellaneous

Can use both ATP and GTP as phosphoryl donors. Phosphorylation by casein kinase 2 has been estimated to represent up to one quarter of the eukaryotic phosphoproteome.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Constitutively active protein kinase whose activity is not directly affected by phosphorylation. Seems to be regulated by level of expression and localization (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei157Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein N-terminus binding Source: UniProtKB
  • protein serine/threonine kinase activity Source: ARUK-UCL

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle, Transcription, Transcription regulation, Wnt signaling pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 2681
ReactomeiR-HSA-1483191 Synthesis of PC
R-HSA-201688 WNT mediated activation of DVL
R-HSA-2514853 Condensation of Prometaphase Chromosomes
R-HSA-445144 Signal transduction by L1
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-6814122 Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding
R-HSA-8934903 Receptor Mediated Mitophagy
R-HSA-8939243 RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known
R-HSA-8948751 Regulation of PTEN stability and activity
SignaLinkiP19784
SIGNORiP19784

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase II subunit alpha' (EC:2.7.11.1)
Short name:
CK II alpha'
Gene namesi
Name:CSNK2A2
Synonyms:CK2A2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000070770.8
HGNCiHGNC:2459 CSNK2A2
MIMi115442 gene
neXtProtiNX_P19784

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi1459
OpenTargetsiENSG00000070770
PharmGKBiPA26959

Chemistry databases

ChEMBLiCHEMBL4070
DrugBankiDB07546 [1-(6-{6-[(1-methylethyl)amino]-1H-indazol-1-yl}pyrazin-2-yl)-1H-pyrrol-3-yl]acetic acid
GuidetoPHARMACOLOGYi1550

Polymorphism and mutation databases

BioMutaiCSNK2A2
DMDMi125266

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000858911 – 350Casein kinase II subunit alpha'Add BLAST350

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13PhosphotyrosineCombined sources1
Modified residuei18PhosphoserineCombined sources1
Modified residuei21PhosphoserineCombined sources1
Modified residuei97N6-acetyllysineCombined sources1
Modified residuei288PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP19784
MaxQBiP19784
PaxDbiP19784
PeptideAtlasiP19784
PRIDEiP19784
ProteomicsDBi53686

2D gel databases

SWISS-2DPAGEiP19784

PTM databases

iPTMnetiP19784
PhosphoSitePlusiP19784

Expressioni

Gene expression databases

BgeeiENSG00000070770
CleanExiHS_CSNK2A2
ExpressionAtlasiP19784 baseline and differential
GenevisibleiP19784 HS

Organism-specific databases

HPAiHPA077719

Interactioni

Subunit structurei

Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). The tetramer can exist as a combination of 2 alpha/2 beta, 2 alpha'/2 beta or 1 alpha/1 alpha'/2 beta subunits. Also part of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, which forms following UV irradiation. Interacts with RNPS1.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein N-terminus binding Source: UniProtKB

Protein-protein interaction databases

BioGridi107842, 226 interactors
CORUMiP19784
DIPiDIP-318N
IntActiP19784, 158 interactors
MINTiP19784
STRINGi9606.ENSP00000262506

Chemistry databases

BindingDBiP19784

Structurei

Secondary structure

1350
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 13Combined sources3
Turni14 – 19Combined sources6
Helixi22 – 25Combined sources4
Helixi27 – 29Combined sources3
Helixi37 – 39Combined sources3
Beta strandi40 – 49Combined sources10
Beta strandi52 – 59Combined sources8
Turni60 – 62Combined sources3
Beta strandi65 – 71Combined sources7
Helixi76 – 88Combined sources13
Turni89 – 91Combined sources3
Beta strandi98 – 103Combined sources6
Turni105 – 107Combined sources3
Beta strandi110 – 115Combined sources6
Helixi122 – 126Combined sources5
Helixi131 – 150Combined sources20
Helixi160 – 162Combined sources3
Beta strandi163 – 166Combined sources4
Turni167 – 170Combined sources4
Beta strandi171 – 174Combined sources4
Helixi196 – 198Combined sources3
Helixi201 – 204Combined sources4
Helixi213 – 228Combined sources16
Beta strandi231 – 234Combined sources4
Helixi239 – 250Combined sources12
Helixi252 – 262Combined sources11
Helixi270 – 273Combined sources4
Helixi282 – 285Combined sources4
Turni288 – 290Combined sources3
Helixi291 – 293Combined sources3
Helixi296 – 305Combined sources10
Helixi310 – 312Combined sources3
Helixi316 – 320Combined sources5
Helixi323 – 325Combined sources3
Helixi326 – 330Combined sources5

3D structure databases

ProteinModelPortaliP19784
SMRiP19784
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19784

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 325Protein kinasePROSITE-ProRule annotationAdd BLAST286

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0668 Eukaryota
ENOG410XNPP LUCA
GeneTreeiENSGT00390000004215
HOGENOMiHOG000233021
HOVERGENiHBG107282
KOiK03097
OMAiNREYWDY
OrthoDBiEOG091G0AZY
PhylomeDBiP19784
TreeFamiTF300483

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P19784-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGPAAGSRA RVYAEVNSLR SREYWDYEAH VPSWGNQDDY QLVRKLGRGK
60 70 80 90 100
YSEVFEAINI TNNERVVVKI LKPVKKKKIK REVKILENLR GGTNIIKLID
110 120 130 140 150
TVKDPVSKTP ALVFEYINNT DFKQLYQILT DFDIRFYMYE LLKALDYCHS
160 170 180 190 200
KGIMHRDVKP HNVMIDHQQK KLRLIDWGLA EFYHPAQEYN VRVASRYFKG
210 220 230 240 250
PELLVDYQMY DYSLDMWSLG CMLASMIFRR EPFFHGQDNY DQLVRIAKVL
260 270 280 290 300
GTEELYGYLK KYHIDLDPHF NDILGQHSRK RWENFIHSEN RHLVSPEALD
310 320 330 340 350
LLDKLLRYDH QQRLTAKEAM EHPYFYPVVK EQSQPCADNA VLSSGLTAAR
Length:350
Mass (Da):41,213
Last modified:February 1, 1991 - v1
Checksum:i3ECB92F6BD3DD7F1
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_040416188E → A1 PublicationCorresponds to variant dbSNP:rs55911801Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55268 mRNA Translation: AAA51548.1
BC008812 mRNA Translation: AAH08812.1
CCDSiCCDS10794.1
PIRiB35838
RefSeqiNP_001887.1, NM_001896.2
UniGeneiHs.82201

Genome annotation databases

EnsembliENST00000262506; ENSP00000262506; ENSG00000070770
GeneIDi1459
KEGGihsa:1459
UCSCiuc002enc.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiCSK22_HUMAN
AccessioniPrimary (citable) accession number: P19784
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: July 18, 2018
This is version 196 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

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