UniProtKB - P19736 (PRP9_YEAST)
Pre-mRNA-splicing factor PRP9
PRP9
Functioni
mRNA splicing factors, PRP9, PRP11, and PRP21, are necessary for binding of the U2 snRNP to the pre-mRNA in an early step of spliceosome assembly.
Miscellaneous
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 280 – 310 | Matrin-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 421 – 452 | Matrin-type 2PROSITE-ProRule annotationAdd BLAST | 32 |
GO - Molecular functioni
- RNA binding Source: SGD
- zinc ion binding Source: InterPro
GO - Biological processi
- mRNA 5'-splice site recognition Source: GO_Central
- mRNA splicing, via spliceosome Source: SGD
Keywordsi
Biological process | mRNA processing, mRNA splicing |
Ligand | Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Pre-mRNA-splicing factor PRP9 |
Gene namesi | Name:PRP9 Ordered Locus Names:YDL030W ORF Names:D2773 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000002188, PRP9 |
VEuPathDBi | FungiDB:YDL030W |
Subcellular locationi
Nucleus
Nucleus
- spliceosomal complex Source: GO_Central
- U2-type prespliceosome Source: SGD
Keywords - Cellular componenti
Nucleus, SpliceosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000174321 | 1 – 530 | Pre-mRNA-splicing factor PRP9Add BLAST | 530 |
Proteomic databases
MaxQBi | P19736 |
PaxDbi | P19736 |
PRIDEi | P19736 |
Interactioni
Subunit structurei
Belongs to the CWC complex (or CEF1-associated complex), a spliceosome sub-complex reminiscent of a late-stage spliceosome composed of the U2, U5 and U6 snRNAs and at least BUD13, BUD31, BRR2, CDC40, CEF1, CLF1, CUS1, CWC2, CWC15, CWC21, CWC22, CWC23, CWC24, CWC25, CWC27, ECM2, HSH155, IST3, ISY1, LEA1, MSL1, NTC20, PRP8, PRP9, PRP11, PRP19, PRP21, PRP22, PRP45, PRP46, SLU7, SMB1, SMD1, SMD2, SMD3, SMX2, SMX3, SNT309, SNU114, SPP2, SYF1, SYF2, RSE1 and YJU2.
1 PublicationProtein-protein interaction databases
BioGRIDi | 32026, 518 interactors |
ComplexPortali | CPX-1648, SF3A complex CPX-1651, PRP19-associated complex CPX-26, U2 small nuclear ribonucleoprotein complex |
DIPi | DIP-651N |
IntActi | P19736, 23 interactors |
MINTi | P19736 |
STRINGi | 4932.YDL030W |
Miscellaneous databases
RNActi | P19736, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P19736 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 367 – 388 | DisorderedSequence analysisAdd BLAST | 22 | |
Regioni | 488 – 516 | DisorderedSequence analysisAdd BLAST | 29 |
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 280 – 310 | Matrin-type 1PROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 421 – 452 | Matrin-type 2PROSITE-ProRule annotationAdd BLAST | 32 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG2636, Eukaryota |
GeneTreei | ENSGT00530000063402 |
HOGENOMi | CLU_027160_1_1_1 |
InParanoidi | P19736 |
OMAi | RYPNEQA |
Family and domain databases
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR031590, PRP9_N IPR031776, SF3A3 IPR031774, SF3A3_dom IPR024598, SF3a60/Prp9_C IPR022755, Znf_C2H2_jaz IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR12786:SF2, PTHR12786:SF2, 1 hit |
Pfami | View protein in Pfam PF16958, PRP9_N, 1 hit PF16837, SF3A3, 1 hit PF11931, SF3a60_Prp9_C, 1 hit PF12171, zf-C2H2_jaz, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 2 hits SM00451, ZnF_U1, 2 hits |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50171, ZF_MATRIN, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNLLETRRSL LEEMEIIENA IAERIQRNPE LYYHYIQESS KVFPDTKLPR
60 70 80 90 100
SSLIAENKIY KFKKVKRKRK QIILQQHEIN IFLRDYQEKQ QTFNKINRPE
110 120 130 140 150
ETQEDDKDLP NFERKLQQLE KELKNEDENF ELDINSKKDK YALFSSSSDP
160 170 180 190 200
SRRTNILSDR ARDLDLNEIF TRDEQYGEYM ELEQFHSLWL NVIKRGDCSL
210 220 230 240 250
LQFLDILELF LDDEKYLLTP PMDRKNDRYM AFLLKLSKYV ETFFFKSYAL
260 270 280 290 300
LDAAAVENLI KSDFEHSYCR GSLRSEAKGI YCPFCSRWFK TSSVFESHLV
310 320 330 340 350
GKIHKKNESK RRNFVYSEYK LHRYLKYLND EFSRTRSFVE RKLAFTANER
360 370 380 390 400
MAEMDILTQK YEAPAYDSTE KEGAEQVDGE QRDGQLQEEH LSGKSFDMPL
410 420 430 440 450
GPDGLPMPYW LYKLHGLDRE YRCEICSNKV YNGRRTFERH FNEERHIYHL
460 470 480 490 500
RCLGIEPSSV FKGITKIKEA QELWKNMQGQ SQLTSIAAVP PKPNPSQLKV
510 520 530
PTELELEEED EEGNVMSKKV YDELKKQGLV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53466 Genomic DNA Translation: CAA37560.1 Z71781 Genomic DNA Translation: CAA96459.1 Z74078 Genomic DNA Translation: CAA98589.1 BK006938 Genomic DNA Translation: DAA11822.1 |
PIRi | S12320 |
RefSeqi | NP_010254.1, NM_001180089.1 |
Genome annotation databases
EnsemblFungii | YDL030W_mRNA; YDL030W; YDL030W |
GeneIDi | 851531 |
KEGGi | sce:YDL030W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X53466 Genomic DNA Translation: CAA37560.1 Z71781 Genomic DNA Translation: CAA96459.1 Z74078 Genomic DNA Translation: CAA98589.1 BK006938 Genomic DNA Translation: DAA11822.1 |
PIRi | S12320 |
RefSeqi | NP_010254.1, NM_001180089.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4DGW | X-ray | 3.11 | A | 1-389 | [»] | |
5NRL | electron microscopy | 7.20 | T | 1-530 | [»] | |
5ZWM | electron microscopy | 3.40 | u | 1-530 | [»] | |
5ZWO | electron microscopy | 3.90 | u | 1-530 | [»] | |
6G90 | electron microscopy | 4.00 | T | 1-530 | [»] | |
7OQB | electron microscopy | 9.00 | T | 1-530 | [»] | |
7OQE | electron microscopy | 5.90 | T | 1-530 | [»] | |
SMRi | P19736 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 32026, 518 interactors |
ComplexPortali | CPX-1648, SF3A complex CPX-1651, PRP19-associated complex CPX-26, U2 small nuclear ribonucleoprotein complex |
DIPi | DIP-651N |
IntActi | P19736, 23 interactors |
MINTi | P19736 |
STRINGi | 4932.YDL030W |
Proteomic databases
MaxQBi | P19736 |
PaxDbi | P19736 |
PRIDEi | P19736 |
Genome annotation databases
EnsemblFungii | YDL030W_mRNA; YDL030W; YDL030W |
GeneIDi | 851531 |
KEGGi | sce:YDL030W |
Organism-specific databases
SGDi | S000002188, PRP9 |
VEuPathDBi | FungiDB:YDL030W |
Phylogenomic databases
eggNOGi | KOG2636, Eukaryota |
GeneTreei | ENSGT00530000063402 |
HOGENOMi | CLU_027160_1_1_1 |
InParanoidi | P19736 |
OMAi | RYPNEQA |
Miscellaneous databases
PROi | PR:P19736 |
RNActi | P19736, protein |
Family and domain databases
InterProi | View protein in InterPro IPR000690, Matrin/U1-C_Znf_C2H2 IPR003604, Matrin/U1-like-C_Znf_C2H2 IPR031590, PRP9_N IPR031776, SF3A3 IPR031774, SF3A3_dom IPR024598, SF3a60/Prp9_C IPR022755, Znf_C2H2_jaz IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
PANTHERi | PTHR12786:SF2, PTHR12786:SF2, 1 hit |
Pfami | View protein in Pfam PF16958, PRP9_N, 1 hit PF16837, SF3A3, 1 hit PF11931, SF3a60_Prp9_C, 1 hit PF12171, zf-C2H2_jaz, 1 hit |
SMARTi | View protein in SMART SM00355, ZnF_C2H2, 2 hits SM00451, ZnF_U1, 2 hits |
SUPFAMi | SSF57667, SSF57667, 1 hit |
PROSITEi | View protein in PROSITE PS50171, ZF_MATRIN, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PRP9_YEAST | |
Accessioni | P19736Primary (citable) accession number: P19736 Secondary accession number(s): D6VRW2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | February 23, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome IV
Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families