UniProtKB - P19712 (POLG_CSFVA)
Genome polyprotein
Functioni
Leader cysteine autoprotease that cleaves itself from the nascent polyprotein during translation of the viral mRNA. Once released, plays a role in the inhibition of host innate immune response by interacting with host IRF3 and inducing its proteasomal degradation.
3 PublicationsPackages viral RNA to form a viral nucleocapsid and thereby protects viral RNA. Plays also a role in transcription regulation. Protects the incoming virus against IFN-induced effectors.
2 PublicationsPlays a role in viral entry. Interacts with host RPSA that acts as a cellular attachment receptor for the virus. Possesses also intrinsic ribonuclease (RNase) activity that can inhibit the production of type I interferon and assist in the development of persistent infections.
5 PublicationsPlays a role in cell attachment and subsequent fusion of viral and cellular membranes. Therefore, mediates together with envelope glycoprotein E2 the viral entry.
1 PublicationPlays a role in cell attachment and subsequent fusion of viral and cellular membranes. Therefore, mediates together with envelope glycoprotein E1 the viral entry.
1 PublicationPlays an essential role in the virus replication cycle by acting as a viroporin. Forms ion conductive pores, which alters the cell permeability allowing the transport of ions and other small molecules.
2 PublicationsAutoprotease that associates with the host chaperone JIV and cleaves the NS2-3 protein between NS2 and NS3. Plays also a role in the formation of infectious particles.
1 PublicationPlays a role in the regulation of viral RNA replication.
1 PublicationMultifunctional protein that contains an N-terminal protease and a C-terminal helicase, playing essential roles in viral polyprotein processing and viral genome replication. The chymotrypsin-like serine protease activity utilizes NS4A as an essential cofactor and catalyzes the cleavage of the polyprotein leading to the release of NS4A, NS4B, NS5A, and NS5B. Plays a role in the inhibition of host NF-kappa-B activation by interacting with and inhibiting host TRAF6. Interacts with NS5B to enhance RNA-dependent RNA polymerase activity.
2 PublicationsActs as a cofactor for the NS3 protease activity.
1 PublicationInduces a specific membrane alteration that serves as a scaffold for the virus replication complex (By similarity).
Antagonizes host cell apoptosis by interacting with host ferritin heavy chain. The ORF4 protein physically binds host FTH1/FHC, resulting in the reduction of FTH1 protein levels in host cells. Reduction of FTH1 concentration further inhibits the accumulation of reactive oxygen in host cells, leading to reduced apoptosis (PubMed:29844394) (By similarity).
By similarity1 PublicationRegulates viral RNA replication by interacting with the 3'-untranslated region of viral RNA in a dose-dependent manner. At small concentrations promotes viral synthesis by interacting with the polymerase NS5B while at large concentrations, inhibits replication.
2 PublicationsReplicates the viral (+) and (-) genome.
PROSITE-ProRule annotationCatalytic activityi
- Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites. EC:3.4.21.113
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 49 | For N-terminal protease activityPROSITE-ProRule annotation1 Publication | 1 | |
Active sitei | 69 | For N-terminal protease activityPROSITE-ProRule annotation2 Publications | 1 | |
Active sitei | 1447 | For cysteine protease NS2 activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1461 | For cysteine protease NS2 activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1512 | For cysteine protease NS2 activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1658 | Charge relay system; for serine protease NS3 activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1695 | Charge relay system; for serine protease NS3 activityPROSITE-ProRule annotation | 1 | |
Active sitei | 1752 | Charge relay system; for serine protease NS3 activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- cysteine-type endopeptidase activity Source: InterPro
- cysteine-type peptidase activity Source: UniProtKB
- ion channel activity Source: UniProtKB-KW
- ribonuclease activity Source: UniProtKB
- ribonuclease T2 activity Source: InterPro
- RNA binding Source: InterPro
- RNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- RNA helicase activity Source: UniProtKB-EC
- serine-type endopeptidase activity Source: InterPro
- serine-type exopeptidase activity Source: InterPro
GO - Biological processi
- fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
- induction by virus of host autophagy Source: UniProtKB-KW
- negative regulation of interferon-beta production Source: AgBase
- pore formation by virus in membrane of host cell Source: UniProtKB
- positive regulation of proteasomal protein catabolic process Source: AgBase
- protein complex oligomerization Source: UniProtKB-KW
- protein localization to cytoplasmic stress granule Source: AgBase
- proteolysis Source: UniProtKB
- regulation of gene expression Source: UniProtKB
- RNA phosphodiester bond hydrolysis Source: UniProtKB
- suppression by virus of host NF-kappaB cascade Source: UniProtKB
- suppression by virus of host TRAF activity Source: UniProtKB
- suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB
- suppression by virus of host type I interferon production Source: AgBase
- suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity Source: UniProtKB
- viral entry into host cell Source: UniProtKB-KW
- viral protein processing Source: InterPro
- viral RNA genome replication Source: InterPro
- virion attachment to host cell Source: UniProtKB-KW
Keywordsi
Enzyme and pathway databases
BRENDAi | 3.4.21.113, 1439 |
Protein family/group databases
MEROPSi | S31.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Genome polyproteinCleaved into the following 13 chains: Alternative name(s): Autoprotease p20 Alternative name(s): Core protein Alternative name(s): gp44/48 Alternative name(s): gp33 Alternative name(s): gp55 Viroporin p71 Publication Alternative name(s): Non-structural protein 2 Serine protease NS3 (EC:3.4.21.113, EC:3.6.1.15, EC:3.6.4.13) Alternative name(s): Non-structural protein 3 Alternative name(s): NS5B |
Organismi | Classical swine fever virus (strain Alfort) (CSFV) (Hog cholera virus) |
Taxonomic identifieri | 11097 [NCBI] |
Taxonomic lineagei | Viruses › Riboviria › Orthornavirae › Kitrinoviricota › Flasuviricetes › Amarillovirales › Flaviviridae › Pestivirus › |
Virus hosti | Sus scrofa (Pig) [TaxID: 9823] |
Proteomesi |
|
Subcellular locationi
- Host cytoplasm 1 Publication
- Virion 1 Publication
- Host cell membrane 1 Publication; Peripheral membrane protein
- Virion membrane 1 Publication; Peripheral membrane protein Curated Note: The C-terminus membrane anchor of Erns represents an amphipathic helix embedded in plane into the membrane.
- Host cell surface 1 Publication
- Virion membrane 1 Publication
- Host membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation
- Host cytoplasm 1 Publication
- Host cytoplasm 2 Publications
- Host cytoplasm 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1140 – 1164 | HelicalPROSITE-ProRule annotationAdd BLAST | 25 | |
Transmembranei | 1189 – 1209 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1217 – 1237 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1247 – 1267 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1281 – 1301 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1360 – 1380 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 | |
Transmembranei | 1568 – 1588 | HelicalPROSITE-ProRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Host cell membrane, Host cytoplasm, Host membrane, Membrane, VirionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 22 | E → V: Almost complete loss of cleavage between N-pro and C. 1 Publication | 1 | |
Mutagenesisi | 40 | H → L: No effect. 1 Publication | 1 | |
Mutagenesisi | 49 | H → L: Complete loss of cleavage between N-pro and C. 1 Publication | 1 | |
Mutagenesisi | 69 | C → A: Complete loss of cleavage between N-pro and C. 1 Publication | 1 | |
Mutagenesisi | 69 | C → S: Complete loss of cleavage between N-pro and C. 1 Publication | 1 | |
Mutagenesisi | 99 | H → L: No effect. 1 Publication | 1 | |
Mutagenesisi | 112 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 112 | C → S: No effect. 1 Publication | 1 | |
Mutagenesisi | 130 | H → L: No effect. 1 Publication | 1 | |
Mutagenesisi | 134 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 134 | C → S: No effect. 1 Publication | 1 | |
Mutagenesisi | 138 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 138 | C → S: No effect. 1 Publication | 1 | |
Mutagenesisi | 161 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 161 | C → S: No effect. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000450893 | 1 – 3898 | Genome polyproteinAdd BLAST | 3898 | |
ChainiPRO_0000038050 | 1 – 168 | N-terminal proteaseAdd BLAST | 168 | |
ChainiPRO_0000038051 | 169 – 267 | Capsid protein CAdd BLAST | 99 | |
ChainiPRO_0000038052 | 268 – 494 | E(rns) glycoproteinAdd BLAST | 227 | |
ChainiPRO_0000038053 | 495 – 656 | Envelope glycoprotein E1Add BLAST | 162 | |
ChainiPRO_0000038054 | 657 – 1062 | Envelope glycoprotein E2Add BLAST | 406 | |
ChainiPRO_0000038055 | 1063 – 1132 | Viroporin p7By similarityAdd BLAST | 70 | |
ChainiPRO_0000038056 | 1133 – 2272 | Non-structural protein 2-3By similarityAdd BLAST | 1140 | |
ChainiPRO_0000349361 | 1133 – 1589 | Cysteine protease NS2PROSITE-ProRule annotationAdd BLAST | 457 | |
ChainiPRO_0000038057 | 1590 – 2272 | Serine protease NS3By similarityAdd BLAST | 683 | |
ChainiPRO_0000038058 | 2273 – 2336 | Non-structural protein 4ABy similarityAdd BLAST | 64 | |
ChainiPRO_0000038059 | 2337 – 2683 | Non-structural protein 4BBy similarityAdd BLAST | 347 | |
ChainiPRO_0000038060 | 2684 – 3180 | Non-structural protein 5ABy similarityAdd BLAST | 497 | |
ChainiPRO_0000038061 | 3181 – 3898 | RNA-directed RNA polymeraseBy similarityAdd BLAST | 718 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 157 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 269 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 274 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 278 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 293 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 332 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 362 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 367 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 410 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 425 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 500 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 594 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 805 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 810 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 874 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 918 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 949 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 986 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 1713 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 2134 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 2217 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 2494 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 2787 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 2815 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 2891 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 3211 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 3316 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 3689 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 3698 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 | |
Glycosylationi | 3794 | N-linked (GlcNAc...) asparagine; by hostSequence analysis | 1 |
Post-translational modificationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 168 – 169 | Cleavage; by autolysisPROSITE-ProRule annotation | 2 | |
Sitei | 267 – 268 | Cleavage; by host signal peptidase | 2 | |
Sitei | 494 – 495 | Cleavage | 2 | |
Sitei | 656 – 657 | Cleavage; by host signal peptidase | 2 | |
Sitei | 1062 – 1063 | Cleavage; by host signal peptidase; partialBy similarity | 2 | |
Sitei | 1132 – 1133 | Cleavage; by host signal peptidaseBy similarity | 2 | |
Sitei | 1589 – 1590 | Cleavage; partial; cysteine protease NS2PROSITE-ProRule annotation | 2 | |
Sitei | 2272 – 2273 | Cleavage; by serine protease NS3By similarity | 2 | |
Sitei | 2336 – 2337 | Cleavage; by serine protease NS3By similarity | 2 | |
Sitei | 2683 – 2684 | Cleavage; by serine protease NS3By similarity | 2 | |
Sitei | 3180 – 3181 | Cleavage; by serine protease NS3By similarity | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PRIDEi | P19712 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Interacts with host TRAF6; this interaction inhibits host NF-kappa-B pathway.
Interacts with NS5B; this interaction enhances RNA-dependent RNA polymerase activity.
Interacts with protein NS4A.
CuratedInteracts with NS5A; this interaction promotes viral replication.
2 PublicationsBinary interactionsi
N-terminal protease (PRO_0000038050)
With | #Exp. | IntAct |
---|---|---|
DHX9 [Q08211] from Homo sapiens. | 6 | EBI-10901281,EBI-352022 |
IRF3 [Q764M6] from Sus scrofa. | 4 | EBI-10901281,EBI-12512266 |
YBX1 [P67809] from Homo sapiens. | 4 | EBI-10901281,EBI-354065 |
Non-structural protein 5A (PRO_0000038060)
With | #Exp. | IntAct |
---|---|---|
FKBP8 [F1S912] from Sus scrofa. | 4 | EBI-12513719,EBI-12512146 |
Protein-protein interaction databases
IntActi | P19712, 96 interactors |
Structurei
Secondary structure
3D structure databases
SMRi | P19712 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 168 | Peptidase C53PROSITE-ProRule annotationAdd BLAST | 168 | |
Domaini | 1441 – 1589 | Peptidase C74PROSITE-ProRule annotationAdd BLAST | 149 | |
Domaini | 1590 – 1763 | Peptidase S31PROSITE-ProRule annotationAdd BLAST | 174 | |
Domaini | 1802 – 1960 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 159 | |
Domaini | 1978 – 2179 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 202 | |
Domaini | 3519 – 3642 | RdRp catalyticPROSITE-ProRule annotationAdd BLAST | 124 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 32 – 54 | DisorderedSequence analysisAdd BLAST | 23 | |
Regioni | 170 – 206 | DisorderedSequence analysisAdd BLAST | 37 | |
Regioni | 221 – 242 | DisorderedSequence analysisAdd BLAST | 22 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 173 – 205 | Basic and acidic residuesSequence analysisAdd BLAST | 33 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
Gene3Di | 2.30.140.40, 1 hit 2.60.320.20, 1 hit 2.60.40.3000, 1 hit 2.60.40.4200, 1 hit 3.30.70.270, 2 hits 3.40.50.300, 2 hits 3.90.730.10, 1 hit |
InterProi | View protein in InterPro IPR021824, Capsid-C_pestivirus IPR011492, DEAD_Flavivir IPR043502, DNA/RNA_pol_sf IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR022120, NS2 IPR030399, NS2_C74 IPR027417, P-loop_NTPase IPR008751, Peptidase_C53 IPR042542, Peptidase_C53_interaction IPR032521, Pestivirus_E2 IPR042309, Pestivirus_E2_A IPR042310, Pestivirus_E2_B IPR042311, Pestivirus_E2_D IPR000280, Pestivirus_NS3_S31 IPR043128, Rev_trsase/Diguanyl_cyclase IPR007094, RNA-dir_pol_PSvirus IPR002166, RNA_pol_HCV IPR036430, RNase_T2-like_sf IPR033130, RNase_T2_His_AS_2 |
Pfami | View protein in Pfam PF11889, DUF3409, 1 hit PF07652, Flavi_DEAD, 1 hit PF00271, Helicase_C, 1 hit PF05550, Peptidase_C53, 1 hit PF12387, Peptidase_C74, 1 hit PF05578, Peptidase_S31, 1 hit PF16329, Pestivirus_E2, 1 hit PF00998, RdRP_3, 1 hit |
PRINTSi | PR00729, CDVENDOPTASE |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF55895, SSF55895, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51692, PESTIVIRUS_NS2_PRO, 1 hit PS51535, PESTIVIRUS_NS3PRO, 1 hit PS51876, PV_NPRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit PS00531, RNASE_T2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MELNHFELLY KTSKQKPVGV EEPVYDTAGR PLFGNPSEVH PQSTLKLPHD
60 70 80 90 100
RGRGDIRTTL RDLPRKGDCR SGNHLGPVSG IYIKPGPVYY QDYTGPVYHR
110 120 130 140 150
APLEFFDEAQ FCEVTKRIGR VTGSDGKLYH IYVCVDGCIL LKLAKRGTPR
160 170 180 190 200
TLKWIRNFTN CPLWVTSCSD DGASGSKDKK PDRMNKGKLK IAPREHEKDS
210 220 230 240 250
KTKPPDATIV VEGVKYQIKK KGKVKGKNTQ DGLYHNKNKP PESRKKLEKA
260 270 280 290 300
LLAWAVITIL LYQPVAAENI TQWNLSDNGT NGIQRAMYLR GVNRSLHGIW
310 320 330 340 350
PEKICKGVPT HLATDTELKE IRGMMDASER TNYTCCRLQR HEWNKHGWCN
360 370 380 390 400
WYNIDPWIQL MNRTQTNLTE GPPDKECAVT CRYDKNTDVN VVTQARNRPT
410 420 430 440 450
TLTGCKKGKN FSFAGTVIEG PCNFNVSVED ILYGDHECGS LLQDTALYLL
460 470 480 490 500
DGMTNTIENA RQGAARVTSW LGRQLSTAGK KLERRSKTWF GAYALSPYCN
510 520 530 540 550
VTRKIGYIWY TNNCTPACLP KNTKIIGPGK FDTNAEDGKI LHEMGGHLSE
560 570 580 590 600
FLLLSLVILS DFAPETASTL YLILHYAIPQ SHEEPEGCDT NQLNLTVKLR
610 620 630 640 650
TEDVVPSSVW NIGKYVCVRP DWWPYETKVA LLFEEAGQVI KLVLRALRDL
660 670 680 690 700
TRVWNSASTT AFLICLIKVL RGQVVQGIIW LLLVTGAQGR LACKEDYRYA
710 720 730 740 750
ISSTNEIGLL GAEGLTTTWK EYSHGLQLDD GTVKAVCTAG SFKVTALNVV
760 770 780 790 800
SRRYLASLHK RALPTSVTFE LLFDGTNPAI EEMDDDFGFG LCPFDTSPVI
810 820 830 840 850
KGKYNTTLLN GSAFYLVCPI GWTGVVECTA VSPTTLRTEV VKTFRRDKPF
860 870 880 890 900
PHRVDCVTTI VEKEDLFHCK LGGNWTCVKG DPVTYKGGQV KQCRWCGFEF
910 920 930 940 950
KEPYGLPHYP IGKCILTNET GYRVVDSTDC NRDGVVISTE GEHECLIGNT
960 970 980 990 1000
TVKVHALDER LGPMPCRPKE IVSSEGPVRK TSCTFNYTKT LRNKYYEPRD
1010 1020 1030 1040 1050
SYFQQYMLKG EYQYWFNLDV TDHHTDYFAE FVVLVVVALL GGRYVLWLIV
1060 1070 1080 1090 1100
TYIILTEQLA AGLQLGQGEV VLIGNLITHT DNEVVVYFLL LYLVIRDEPI
1110 1120 1130 1140 1150
KKWILLLFHA MTNNPVKTIT VALLMISGVA KGGKIDGGWQ RQPVTSFDIQ
1160 1170 1180 1190 1200
LALAVVVVVV MLLAKRDPTT FPLVITVATL RTAKITNGFS TDLVIATVSA
1210 1220 1230 1240 1250
ALLTWTYISD YYKYKTWLQY LVSTVTGIFL IRVLKGIGEL DLHAPTLPSH
1260 1270 1280 1290 1300
RPLFYILVYL ISTAVVTRWN LDVAGLLLQC VPTLLMVFTM WADILTLILI
1310 1320 1330 1340 1350
LPTYELTKLY YLKEVKIGAE RGWLWKTNYK RVNDIYEVDQ TSEGVYLFPS
1360 1370 1380 1390 1400
KQRTSAITST MLPLIKAILI SCISNKWQLI YLLYLIFEVS YYLHKKVIDE
1410 1420 1430 1440 1450
IAGGTNFVSR LVAALIEVNW AFDNEEVKGL KKFFLLSSRV KELIIKHKVR
1460 1470 1480 1490 1500
NEVVVRWFGD EEIYGMPKLI GLVKAATLSR NKHCMLCTVC EDRDWRGETC
1510 1520 1530 1540 1550
PKCGRFGPPV VCGMTLADFE EKHYKRIFIR EDQSGGPLRE EHAGYLQYKA
1560 1570 1580 1590 1600
RGQLFLRNLP VLATKVKMLL VGNLGTEIGD LEHLGWVLRG PAVCKKVTEH
1610 1620 1630 1640 1650
ERCTTSIMDK LTAFFGVMPR GTTPRAPVRF PTSLLKIRRG LETGWAYTHQ
1660 1670 1680 1690 1700
GGISSVDHVT CGKDLLVCDT MGRTRVVCQS NNKMTDESEY GVKTDSGCPE
1710 1720 1730 1740 1750
GARCYVFNPE AVNISGTKGA MVHLQKTGGE FTCVTASGTP AFFDLKNLKG
1760 1770 1780 1790 1800
WSGLPIFEAS SGRVVGRVKV GKNEDSKPTK LMSGIQTVSK SATDLTEMVK
1810 1820 1830 1840 1850
KITTMNRGEF RQITLATGAG KTTELPRSVI EEIGRHKRVL VLIPLRAAAE
1860 1870 1880 1890 1900
SVYQYMRQKH PSIAFNLRIG EMKEGDMATG ITYASYGYFC QMSQPKLRAA
1910 1920 1930 1940 1950
MVEYSFIFLD EYHCATPEQL AIMGKIHRFS ENLRVVAMTA TPAGTVTTTG
1960 1970 1980 1990 2000
QKHPIEEFIA PEVMKGEDLG SEYLDIAGLK IPVEEMKNNM LVFVPTRNMA
2010 2020 2030 2040 2050
VEAAKKLKAK GYNSGYYYSG EDPSNLRVVT SQSPYVVVAT NAIESGVTLP
2060 2070 2080 2090 2100
DLDVVVDTGL KCEKRIRLSP KMPFIVTGLK RMAVTIGEQA QRRGRVGRVK
2110 2120 2130 2140 2150
PGRYYRSQET PVGSKDYHYD LLQAQRYGIE DGINITKSFR EMNYDWSLYE
2160 2170 2180 2190 2200
EDSLMITQLE ILNNLLISEE LPMAVKNIMA RTDHPEPIQL AYNSYETQVP
2210 2220 2230 2240 2250
VLFPKIRNGE VTDTYDNYTF LNARKLGDDV PPYVYATEDE DLAVELLGLD
2260 2270 2280 2290 2300
WPDPGNQGTV EAGRALKQVV GLSTAENALL VALFGYVGYQ ALSKRHIPVV
2310 2320 2330 2340 2350
TDIYSVEDHR LEDTTHLQYA PNAIKTEGKE TELKELAQGD VQRCVEAVTN
2360 2370 2380 2390 2400
YAREGIQFMK SQALKVRETP TYKETMNTVA DYVKKFIEAL TDSKEDIIKY
2410 2420 2430 2440 2450
GLWGAHTALY KSIGARLGHE TAFATLVVKW LAFGGESISD HIKQAATDLV
2460 2470 2480 2490 2500
VYYIINRPQF PGDTETQQEG RKFVASLLVS ALATYTYKSW NYNNLSKIVE
2510 2520 2530 2540 2550
PALATLPYAA KALKLFAPTR LESVVILSTA IYKTYLSIRR GKSDGLLGTG
2560 2570 2580 2590 2600
VSAAMEIMSQ NPVSVGIAVM LGVGAVAAHN AIEASEQKRT LLMKVFVKNF
2610 2620 2630 2640 2650
LDQAATDELV KESPEKIIMA LFEAVQTVGN PLRLVYHLYG VFYKGWEAKE
2660 2670 2680 2690 2700
LAQRTAGRNL FTLIMFEAVE LLGVDSEGKI RQLSSNYILE LLYKFRDNIK
2710 2720 2730 2740 2750
SSVREIAISW APAPFSCDWT PTDDRIGLPH ENYLRVETKC PCGYRMKAVK
2760 2770 2780 2790 2800
NCAGELRLLE EGGSFLCRNK FGRGSQNYRV TKYYDDNLSE IKPVIRMEGH
2810 2820 2830 2840 2850
VELYYKGATI KLDFNNSKTV LATDKWEVDH STLVRALKRY TGAGYRGAYL
2860 2870 2880 2890 2900
GEKPNHKHLI QRDCATITKD KVCFIKMKRG CAFTYDLSLH NLTRLIELVH
2910 2920 2930 2940 2950
KNNLEDREIP AVTVTTWLAY TFVNEDIGTI KPTFGEKVTP EKQEEVVLQP
2960 2970 2980 2990 3000
AVVVDTTDVA VTVVGETSTM TTGETPTTFT SLGSDSKVRQ VLKLGVDDGQ
3010 3020 3030 3040 3050
YPGPNQQRAS LLEAIQGVDE RPSVLILGSD KATSNRVKTA KNVKIYRSRD
3060 3070 3080 3090 3100
PLELREMMKR GKILVVALSR VDTALLKFVD YKGTFLTRET LEALSLGKPK
3110 3120 3130 3140 3150
KRDITKAEAQ WLLRLEDQIE ELPDWFAAKE PIFLEANIKR DKYHLVGDIA
3160 3170 3180 3190 3200
TIKEKAKQLG ATDSTKISKE VGAKVYSMKL SNWVIQEENK QGSLAPLFEE
3210 3220 3230 3240 3250
LLQQCPPGGQ NKTTHMVSAY QLAQGNWVPV SCHVFMGTIP ARRTKTHPYE
3260 3270 3280 3290 3300
AYVKLRELVD EHKMKALCGG SGLSKHNEWV IGKVKYQGNL RTKHMLNPGK
3310 3320 3330 3340 3350
VAEQLHREGY RHNVYNKTIG SVMTATGIRL EKLPVVRAQT DTTNFHQAIR
3360 3370 3380 3390 3400
DKIDKEENLQ TPGLHKKLME VFNALKRPEL EASYDAVDWE ELERGINRKG
3410 3420 3430 3440 3450
AAGFFERKNI GEVLDSEKNK VEEVIDSLKK GRNIRYYETA IPKNEKRDVN
3460 3470 3480 3490 3500
DDWTAGDFVD EKKPRVIQYP EAKTRLAITK VMYKWVKQKP VVIPGYEGKT
3510 3520 3530 3540 3550
PLFQIFDKVK KEWDQFQNPV AVSFDTKAWD TQVTTRDLEL IRDIQKFYFK
3560 3570 3580 3590 3600
KKWHKFIDTL TKHMSEVPVI SADGEVYIRK GQRGSGQPDT SAGNSMLNVL
3610 3620 3630 3640 3650
TMVYAFCEAT GVPYKSFDRV AKIHVCGDDG FLITERALGE KFASKGVQIL
3660 3670 3680 3690 3700
YEAGKPQKIT EGDKMKVAYQ FDDIEFCSHT PVQVRWSDNT SSYMPGRNTT
3710 3720 3730 3740 3750
TILAKMATRL DSSGERGTIA YEKAVAFSFL LMYSWNPLIR RICLLVLSTE
3760 3770 3780 3790 3800
LQVRPGKSTT YYYEGDPISA YKEVIGHNLF DLKRTSFEKL AKLNLSMSTL
3810 3820 3830 3840 3850
GVWTRHTSKR LLQDCVNVGT KEGNWLVNAD RLVSSKTGNR YIPGEGHTLQ
3860 3870 3880 3890
GKHYEELILA RKPIGNFEGT DRYNLGPIVN VVLRRLKIMM MALIGRGV
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 387 | T → A. | 1 | |
Natural varianti | 3542 | R → S. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04358 Genomic RNA Translation: AAA43844.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04358 Genomic RNA Translation: AAA43844.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4CBG | X-ray | 2.82 | A/B/C/D | 1782-2280 | [»] | |
4CBH | X-ray | 2.51 | A/B/C/D | 1782-2280 | [»] | |
4CBI | X-ray | 3.00 | A/B/C/D | 1782-2280 | [»] | |
4CBL | X-ray | 3.05 | A/B/C/D | 1792-2280 | [»] | |
4CBM | X-ray | 3.27 | A/B/C/D | 1782-2280 | [»] | |
5MZ4 | X-ray | 3.05 | A/B | 1590-2280 | [»] | |
SMRi | P19712 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P19712, 96 interactors |
Protein family/group databases
MEROPSi | S31.001 |
Proteomic databases
PRIDEi | P19712 |
Enzyme and pathway databases
BRENDAi | 3.4.21.113, 1439 |
Family and domain databases
Gene3Di | 2.30.140.40, 1 hit 2.60.320.20, 1 hit 2.60.40.3000, 1 hit 2.60.40.4200, 1 hit 3.30.70.270, 2 hits 3.40.50.300, 2 hits 3.90.730.10, 1 hit |
InterProi | View protein in InterPro IPR021824, Capsid-C_pestivirus IPR011492, DEAD_Flavivir IPR043502, DNA/RNA_pol_sf IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR022120, NS2 IPR030399, NS2_C74 IPR027417, P-loop_NTPase IPR008751, Peptidase_C53 IPR042542, Peptidase_C53_interaction IPR032521, Pestivirus_E2 IPR042309, Pestivirus_E2_A IPR042310, Pestivirus_E2_B IPR042311, Pestivirus_E2_D IPR000280, Pestivirus_NS3_S31 IPR043128, Rev_trsase/Diguanyl_cyclase IPR007094, RNA-dir_pol_PSvirus IPR002166, RNA_pol_HCV IPR036430, RNase_T2-like_sf IPR033130, RNase_T2_His_AS_2 |
Pfami | View protein in Pfam PF11889, DUF3409, 1 hit PF07652, Flavi_DEAD, 1 hit PF00271, Helicase_C, 1 hit PF05550, Peptidase_C53, 1 hit PF12387, Peptidase_C74, 1 hit PF05578, Peptidase_S31, 1 hit PF16329, Pestivirus_E2, 1 hit PF00998, RdRP_3, 1 hit |
PRINTSi | PR00729, CDVENDOPTASE |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF55895, SSF55895, 1 hit SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51692, PESTIVIRUS_NS2_PRO, 1 hit PS51535, PESTIVIRUS_NS3PRO, 1 hit PS51876, PV_NPRO, 1 hit PS50507, RDRP_SSRNA_POS, 1 hit PS00531, RNASE_T2_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | POLG_CSFVA | |
Accessioni | P19712Primary (citable) accession number: P19712 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | July 11, 2001 | |
Last modified: | May 25, 2022 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families