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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza C virus (strain C/JJ/1950)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-directed RNA polymerase, Transferase
Biological processEukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription
LigandNucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunitUniRule annotation (EC:2.7.7.48UniRule annotation)
Alternative name(s):
Polymerase basic protein 1UniRule annotation
Short name:
PB1UniRule annotation
RNA-directed RNA polymerase subunit P1UniRule annotation
Gene namesi
Name:PB1UniRule annotation
OrganismiInfluenza C virus (strain C/JJ/1950)
Taxonomic identifieri11560 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeGammainfluenzavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Host nucleus UniRule annotation
  • Host cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000787761 – 754RNA-directed RNA polymerase catalytic subunitAdd BLAST754

Post-translational modificationi

Phosphorylated by host PRKCA.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.UniRule annotation

Structurei

3D structure databases

SMRiP19703
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini288 – 484RdRp catalyticUniRule annotationAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni251 – 258Promoter-binding siteUniRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi189 – 197Nuclear localization signalUniRule annotation9
Motifi205 – 218Nuclear localization signalUniRule annotationAdd BLAST14

Sequence similaritiesi

Belongs to the influenza viruses polymerase PB1 family.UniRule annotation

Family and domain databases

HAMAPiMF_04065 INFV_RDRP, 1 hit
InterProiView protein in InterPro
IPR007099 RNA-dir_pol_NSvirus
IPR001407 RNA_pol_PB1_influenza
PfamiView protein in Pfam
PF00602 Flu_PB1, 1 hit
PIRSFiPIRSF000827 RdRPol_OMV, 1 hit
PROSITEiView protein in PROSITE
PS50525 RDRP_SSRNA_NEG_SEG, 1 hit

Sequencei

Sequence statusi: Complete.

P19703-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEINPYLMFL NNDVTSLIST TYPYTGPPPM SHGSSTKYTL ETIKRTYDYS
60 70 80 90 100
RTSVEKTSKV FNIPRRKFCN CLEDKDDLVK PTGNVDISSL LGLAEMMEKR
110 120 130 140 150
MGEGFFKHCV MEAETEILKM HFSRLTEGRQ TYDWTSERNM PAATALQLTV
160 170 180 190 200
DAIKETEGPF KGTTMLEYCN KMIEMLDWKE VKFRKVKTMV RREKDKRSGK
210 220 230 240 250
EIKTKVPVMG IDSIKHDEFL IRALTINTMA KDGERGKLQR RAIATPGMIV
260 270 280 290 300
RPFSKIVETV AQKICEKLKE SGLPVGGNEK KAKLKTTVTS LNARMNSDQF
310 320 330 340 350
AVNITGDNSK WNECQQPEAY LALLAYITKD SSDLMKDLCS VAPVLFCNKF
360 370 380 390 400
VKLGQGIRLS NKRKTKEVII KAEKMGKYKN LMREEYKNLF EPLEKYIQKD
410 420 430 440 450
VCFLPGGMLM GMFNMLSTVL GVSTLCYMDE ELKAKGCFWT GLQSSDDFVL
460 470 480 490 500
FAVASNWSNI HWTIRRFNAV CKLIGINMSL EKSYGSLPEL FEFTSMFFDG
510 520 530 540 550
EFVSNLAMEL PAFTTAGVNE GVDFTAAMSI IKTNMINNSL SPSTALMALR
560 570 580 590 600
ICLQEFRATY RVHPWDSKVK GGRMKIINEF IKTIESKDGL LIADGGKLMN
610 620 630 640 650
NISTLHIPEE VLKFEKMDEQ YRNRVFNPKN PFTNFDKTID IFRAHGPIRV
660 670 680 690 700
EENEAVVSTH SFRTRANRTL LNTDMRAMMA EEKRYQMVCD IFKSVFESAD
710 720 730 740 750
INPPIGAMSI GEAIEEKLLE RAKMKRDIGA IEDSEYEEIK DIIRDAKKAR

IESR
Length:754
Mass (Da):85,984
Last modified:February 1, 1991 - v1
Checksum:i49CBEA8A3E854606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28060 Genomic RNA Translation: AAA43814.1
PIRiB34225 P1IV50

Similar proteinsi

Entry informationi

Entry nameiRDRP_INCJJ
AccessioniPrimary (citable) accession number: P19703
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: April 25, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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