UniProtKB - P19658 (EXO70_YEAST)
Protein
Exocyst complex component EXO70
Gene
EXO70
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Involved in the secretory pathway as part of the exocyst complex which tethers secretory vesicles to the sites of exocytosis. Plays a role in the assembly of the exocyst.1 Publication
Miscellaneous
Present with 4280 molecules/cell in log phase SD medium.1 Publication
GO - Molecular functioni
- GTP-Rho binding Source: SGD
- phosphatidylinositol-4,5-bisphosphate binding Source: SGD
GO - Biological processi
- exocyst assembly Source: SGD
- exocyst localization Source: SGD
- exocytosis Source: SGD
- Golgi to plasma membrane transport Source: SGD
- protein transport Source: UniProtKB-KW
- Rho protein signal transduction Source: SGD
- vesicle tethering involved in exocytosis Source: SGD
Keywordsi
Biological process | Exocytosis, Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-264876, Insulin processing |
Protein family/group databases
TCDBi | 1.F.2.1.1, the octameric exocyst (exocyst) family |
Names & Taxonomyi
Protein namesi | Recommended name: Exocyst complex component EXO70Alternative name(s): Exocyst complex protein of 70 kDa |
Gene namesi | Name:EXO70 Ordered Locus Names:YJL085W ORF Names:J0932 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003621, EXO70 |
VEuPathDBi | FungiDB:YJL085W |
Subcellular locationi
Other locations
- secretory vesicle 1 Publication
- Bud 1 Publication
- Bud neck Curated
Note: Its polarization in the cell depends on EXO84.1 Publication
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cellular bud neck Source: SGD
- cellular bud tip Source: SGD
- cytoplasm Source: SGD
- exocyst Source: SGD
- incipient cellular bud site Source: SGD
- mating projection tip Source: SGD
- prospore membrane Source: SGD
- transport vesicle Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasmic vesiclePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118975 | 1 – 623 | Exocyst complex component EXO70Add BLAST | 623 |
Proteomic databases
MaxQBi | P19658 |
PaxDbi | P19658 |
PRIDEi | P19658 |
PTM databases
iPTMneti | P19658 |
Interactioni
Subunit structurei
The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.
Interacts with RHO3.
3 PublicationsBinary interactionsi
Hide detailsP19658
With | #Exp. | IntAct |
---|---|---|
EXO84 [P38261] | 2 | EBI-6717,EBI-21567 |
RHO3 [Q00245] | 4 | EBI-6717,EBI-15138 |
SEC10 [Q06245] | 2 | EBI-6717,EBI-16504 |
SEC15 [P22224] | 2 | EBI-6717,EBI-16543 |
SEC6 [P32844] | 3 | EBI-6717,EBI-16874 |
GO - Molecular functioni
- GTP-Rho binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 33671, 527 interactors |
ComplexPortali | CPX-1890, Exocyst |
DIPi | DIP-2934N |
IntActi | P19658, 10 interactors |
MINTi | P19658 |
STRINGi | 4932.YJL085W |
Miscellaneous databases
RNActi | P19658, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P19658 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P19658 |
Family & Domainsi
Sequence similaritiesi
Belongs to the EXO70 family.Curated
Phylogenomic databases
eggNOGi | KOG2344, Eukaryota |
GeneTreei | ENSGT00390000003595 |
HOGENOMi | CLU_010236_4_1_1 |
InParanoidi | P19658 |
OMAi | YHDLSTQ |
Family and domain databases
InterProi | View protein in InterPro IPR016159, Cullin_repeat-like_dom_sf IPR004140, Exo70 |
PANTHERi | PTHR12542, PTHR12542, 1 hit |
Pfami | View protein in Pfam PF03081, Exo70, 1 hit |
SUPFAMi | SSF74788, SSF74788, 1 hit |
i Sequence
Sequence statusi: Complete.
P19658-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPAEIDIDEA DVLVLSQELQ KTSKLTFEIN KSLKKIAATS NQSSQLFTPI
60 70 80 90 100
LARNNVLTTL QRNIESTLNS VASVKDLANE ASKYEIILQK GINQVGLKQY
110 120 130 140 150
TQVVHKLDDM LEDIQSGQAN REENSEFHGI LTHLEQLIKR SEAQLRVYFI
160 170 180 190 200
SILNSIKPFD PQINITKKMP FPYYEDQQLG ALSWILDYFH GNSEGSIIQD
210 220 230 240 250
ILVGERSKLI LKCMAFLEPF AKEISTAKNA PYEKGSSGMN SYTEALLGFI
260 270 280 290 300
ANEKSLVDDL YSQYTESKPH VLSQILSPLI SAYAKLFGAN LKIVRSNLEN
310 320 330 340 350
FGFFSFELVE SINDVKKSLR GKELQNYNLL QDCTQEVRQV TQSLFRDAID
360 370 380 390 400
RIIKKANSIS TIPSNNGVTE ATVDTMSRLR KFSEYKNGCL GAMDNITREN
410 420 430 440 450
WLPSNYKEKE YTLQNEALNW EDHNVLLSCF ISDCIDTLAV NLERKAQIAL
460 470 480 490 500
MPNQEPDVAN PNSSKNKHKQ RIGFFILMNL TLVEQIVEKS ELNLMLAGEG
510 520 530 540 550
HSRLERLKKR YISYMVSDWR DLTANLMDSV FIDSSGKKSK DKEQIKEKFR
560 570 580 590 600
KFNEGFEDLV SKTKQYKLSD PSLKVTLKSE IISLVMPMYE RFYSRYKDSF
610 620
KNPRKHIKYT PDELTTVLNQ LVR
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 497 | A → S in AAU09750 (PubMed:17322287).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08787 Genomic DNA Translation: CAA70039.1 Y00473 Genomic DNA Translation: CAA68535.1 X83502 Genomic DNA Translation: CAA58485.1 Z49362 Genomic DNA Translation: CAA89380.1 AY723833 Genomic DNA Translation: AAU09750.1 J03546 Genomic DNA Translation: AAA66919.1 BK006943 Genomic DNA Translation: DAA08714.1 |
PIRi | A27300 |
RefSeqi | NP_012450.1, NM_001181518.1 |
Genome annotation databases
EnsemblFungii | YJL085W_mRNA; YJL085W; YJL085W |
GeneIDi | 853359 |
KEGGi | sce:YJL085W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y08787 Genomic DNA Translation: CAA70039.1 Y00473 Genomic DNA Translation: CAA68535.1 X83502 Genomic DNA Translation: CAA58485.1 Z49362 Genomic DNA Translation: CAA89380.1 AY723833 Genomic DNA Translation: AAU09750.1 J03546 Genomic DNA Translation: AAA66919.1 BK006943 Genomic DNA Translation: DAA08714.1 |
PIRi | A27300 |
RefSeqi | NP_012450.1, NM_001181518.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2B1E | X-ray | 2.00 | A | 63-623 | [»] | |
2B7M | X-ray | 3.50 | A/B/C/D | 63-623 | [»] | |
2PFV | X-ray | 2.10 | A | 63-623 | [»] | |
5YFP | electron microscopy | 4.40 | G | 1-623 | [»] | |
6VKL | electron microscopy | 4.40 | G | 1-623 | [»] | |
SMRi | P19658 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33671, 527 interactors |
ComplexPortali | CPX-1890, Exocyst |
DIPi | DIP-2934N |
IntActi | P19658, 10 interactors |
MINTi | P19658 |
STRINGi | 4932.YJL085W |
Protein family/group databases
TCDBi | 1.F.2.1.1, the octameric exocyst (exocyst) family |
PTM databases
iPTMneti | P19658 |
Proteomic databases
MaxQBi | P19658 |
PaxDbi | P19658 |
PRIDEi | P19658 |
Genome annotation databases
EnsemblFungii | YJL085W_mRNA; YJL085W; YJL085W |
GeneIDi | 853359 |
KEGGi | sce:YJL085W |
Organism-specific databases
SGDi | S000003621, EXO70 |
VEuPathDBi | FungiDB:YJL085W |
Phylogenomic databases
eggNOGi | KOG2344, Eukaryota |
GeneTreei | ENSGT00390000003595 |
HOGENOMi | CLU_010236_4_1_1 |
InParanoidi | P19658 |
OMAi | YHDLSTQ |
Enzyme and pathway databases
Reactomei | R-SCE-264876, Insulin processing |
Miscellaneous databases
EvolutionaryTracei | P19658 |
PROi | PR:P19658 |
RNActi | P19658, protein |
Family and domain databases
InterProi | View protein in InterPro IPR016159, Cullin_repeat-like_dom_sf IPR004140, Exo70 |
PANTHERi | PTHR12542, PTHR12542, 1 hit |
Pfami | View protein in Pfam PF03081, Exo70, 1 hit |
SUPFAMi | SSF74788, SSF74788, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EXO70_YEAST | |
Accessioni | P19658Primary (citable) accession number: P19658 Secondary accession number(s): D6VW98, E9P961 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | February 10, 2021 | |
This is version 162 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families