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Protein

Plasma membrane ATPase 2

Gene

PMA2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Miscellaneous

There are two plasma membrane ATPases in yeast. This is the minor isoform.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4074-aspartylphosphate intermediateBy similarity1
Metal bindingi663MagnesiumBy similarity1
Metal bindingi667MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • proton export across plasma membrane Source: InterPro
  • proton transmembrane transport Source: SGD
  • regulation of intracellular pH Source: GO_Central
  • regulation of pH Source: SGD

Keywordsi

Molecular functionTranslocase
Biological processHydrogen ion transport, Ion transport, Transport
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-33950-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane ATPase 2 (EC:7.1.2.1)
Alternative name(s):
Proton pump 2
Gene namesi
Name:PMA2
Ordered Locus Names:YPL036W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

SGDiS000005957 PMA2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 144CytoplasmicSequence analysisAdd BLAST144
Transmembranei145 – 165Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini166 – 169ExtracellularSequence analysis4
Transmembranei170 – 189Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini190 – 320CytoplasmicSequence analysisAdd BLAST131
Transmembranei321 – 342Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini343 – 353ExtracellularSequence analysisAdd BLAST11
Transmembranei354 – 376Helical; Name=4Sequence analysisAdd BLAST23
Topological domaini377 – 748CytoplasmicSequence analysisAdd BLAST372
Transmembranei749 – 767Helical; Name=5Sequence analysisAdd BLAST19
Topological domaini768 – 783ExtracellularSequence analysisAdd BLAST16
Transmembranei784 – 803Helical; Name=6Sequence analysisAdd BLAST20
Topological domaini804 – 853CytoplasmicSequence analysisAdd BLAST50
Transmembranei854 – 874Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini875 – 886ExtracellularSequence analysisAdd BLAST12
Transmembranei887 – 903Helical; Name=8Sequence analysisAdd BLAST17
Topological domaini904 – 947CytoplasmicSequence analysisAdd BLAST44

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000462721 – 947Plasma membrane ATPase 2Add BLAST947

Proteomic databases

MaxQBiP19657
PaxDbiP19657
PRIDEiP19657

PTM databases

iPTMnetiP19657

Interactioni

Protein-protein interaction databases

BioGridi36143, 374 interactors
DIPiDIP-4036N
IntActiP19657, 4 interactors
MINTiP19657
STRINGi4932.YPL036W

Structurei

3D structure databases

ProteinModelPortaliP19657
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi33 – 47Poly-SerAdd BLAST15
Compositional biasi62 – 91Asp/Glu-rich (acidic)Add BLAST30

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00910000144699
HOGENOMiHOG000160005
InParanoidiP19657
KOiK01535
OMAiHPGDGIY
OrthoDBiEOG092C0HLD

Family and domain databases

CDDicd02076 P-type_ATPase_H, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006534 P-type_ATPase_IIIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01647 ATPase-IIIA_H, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

P19657-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSTEAKQYK EKPSKEYLHA SDGDDPANNS AASSSSSSST STSASSSAAA
60 70 80 90 100
VPRKAAAASA ADDSDSDEDI DQLIDELQSN YGEGDESGEE EVRTDGVHAG
110 120 130 140 150
QRVVPEKDLS TDPAYGLTSD EVARRRKKYG LNQMAEENES LIVKFLMFFV
160 170 180 190 200
GPIQFVMEAA AILAAGLSDW VDVGVICALL LLNASVGFIQ EFQAGSIVDE
210 220 230 240 250
LKKTLANTAT VIRDGQLIEI PANEVVPGEI LQLESGTIAP ADGRIVTEDC
260 270 280 290 300
FLQIDQSAIT GESLAAEKHY GDEVFSSSTV KTGEAFMVVT ATGDNTFVGR
310 320 330 340 350
AAALVGQASG VEGHFTEVLN GIGIILLVLV IATLLLVWTA CFYRTVGIVS
360 370 380 390 400
ILRYTLGITI IGVPVGLPAV VTTTMAVGAA YLAKKQAIVQ KLSAIESLAG
410 420 430 440 450
VEILCSDKTG TLTKNKLSLH EPYTVEGVSP DDLMLTACLA ASRKKKGLDA
460 470 480 490 500
IDKAFLKSLI EYPKAKDALT KYKVLEFHPF DPVSKKVTAV VESPEGERIV
510 520 530 540 550
CVKGAPLFVL KTVEEDHPIP EDVHENYENK VAELASRGFR ALGVARKRGE
560 570 580 590 600
GHWEILGVMP CMDPPRDDTA QTINEARNLG LRIKMLTGDA VGIAKETCRQ
610 620 630 640 650
LGLGTNIYNA ERLGLGGGGD MPGSELADFV ENADGFAEVF PQHKYRVVEI
660 670 680 690 700
LQNRGYLVAM TGDGVNDAPS LKKADTGIAV EGATDAARSA ADIVFLAPGL
710 720 730 740 750
SAIIDALKTS RQIFHRMYSY VVYRIALSLH LEIFLGLWIA ILNNSLDINL
760 770 780 790 800
IVFIAIFADV ATLTIAYDNA PYAPEPVKWN LPRLWGMSII LGIVLAIGSW
810 820 830 840 850
ITLTTMFLPN GGIIQNFGAM NGVMFLQISL TENWLIFVTR AAGPFWSSIP
860 870 880 890 900
SWQLAGAVFA VDIIATMFTL FGWWSENWTD IVSVVRVWIW SIGIFCVLGG
910 920 930 940
FYYIMSTSQA FDRLMNGKSL KEKKSTRSVE DFMAAMQRVS TQHEKSS
Length:947
Mass (Da):102,172
Last modified:October 1, 1996 - v3
Checksum:i24CF0D3EAFD1E9D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti944E → D in AAA83387 (PubMed:2904437).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04421 Genomic DNA Translation: AAA83387.1
U44030 Genomic DNA Translation: AAB68184.1
BK006949 Genomic DNA Translation: DAA11394.1
PIRiS62039 PXBY2P
RefSeqiNP_015289.1, NM_001183850.1

Genome annotation databases

EnsemblFungiiYPL036W; YPL036W; YPL036W
GeneIDi856071
KEGGisce:YPL036W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04421 Genomic DNA Translation: AAA83387.1
U44030 Genomic DNA Translation: AAB68184.1
BK006949 Genomic DNA Translation: DAA11394.1
PIRiS62039 PXBY2P
RefSeqiNP_015289.1, NM_001183850.1

3D structure databases

ProteinModelPortaliP19657
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36143, 374 interactors
DIPiDIP-4036N
IntActiP19657, 4 interactors
MINTiP19657
STRINGi4932.YPL036W

PTM databases

iPTMnetiP19657

Proteomic databases

MaxQBiP19657
PaxDbiP19657
PRIDEiP19657

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL036W; YPL036W; YPL036W
GeneIDi856071
KEGGisce:YPL036W

Organism-specific databases

SGDiS000005957 PMA2

Phylogenomic databases

GeneTreeiENSGT00910000144699
HOGENOMiHOG000160005
InParanoidiP19657
KOiK01535
OMAiHPGDGIY
OrthoDBiEOG092C0HLD

Enzyme and pathway databases

BioCyciYEAST:G3O-33950-MONOMER

Miscellaneous databases

PROiPR:P19657

Family and domain databases

CDDicd02076 P-type_ATPase_H, 1 hit
Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006534 P-type_ATPase_IIIA
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01647 ATPase-IIIA_H, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPMA2_YEAST
AccessioniPrimary (citable) accession number: P19657
Secondary accession number(s): D6W3X8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: November 7, 2018
This is version 177 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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