UniProtKB - P19657 (PMA2_YEAST)
Protein
Plasma membrane ATPase 2
Gene
PMA2
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.
Miscellaneous
There are two plasma membrane ATPases in yeast. This is the minor isoform.
Catalytic activityi
- EC:7.1.2.1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 407 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 663 | MagnesiumBy similarity | 1 | |
Metal bindingi | 667 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- proton-exporting ATPase activity, phosphorylative mechanism Source: SGD
GO - Biological processi
- proton export across plasma membrane Source: InterPro
- proton transmembrane transport Source: SGD
- regulation of intracellular pH Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PMA2 Ordered Locus Names:YPL036W |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YPL036W |
SGDi | S000005957, PMA2 |
Subcellular locationi
Plasma membrane
Mitochondrion
- mitochondrion Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- cell periphery Source: SGD
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 144 | CytoplasmicSequence analysisAdd BLAST | 144 | |
Transmembranei | 145 – 165 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 166 – 169 | ExtracellularSequence analysis | 4 | |
Transmembranei | 170 – 189 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Topological domaini | 190 – 320 | CytoplasmicSequence analysisAdd BLAST | 131 | |
Transmembranei | 321 – 342 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 343 – 353 | ExtracellularSequence analysisAdd BLAST | 11 | |
Transmembranei | 354 – 376 | Helical; Name=4Sequence analysisAdd BLAST | 23 | |
Topological domaini | 377 – 748 | CytoplasmicSequence analysisAdd BLAST | 372 | |
Transmembranei | 749 – 767 | Helical; Name=5Sequence analysisAdd BLAST | 19 | |
Topological domaini | 768 – 783 | ExtracellularSequence analysisAdd BLAST | 16 | |
Transmembranei | 784 – 803 | Helical; Name=6Sequence analysisAdd BLAST | 20 | |
Topological domaini | 804 – 853 | CytoplasmicSequence analysisAdd BLAST | 50 | |
Transmembranei | 854 – 874 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 875 – 886 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 887 – 903 | Helical; Name=8Sequence analysisAdd BLAST | 17 | |
Topological domaini | 904 – 947 | CytoplasmicSequence analysisAdd BLAST | 44 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046272 | 1 – 947 | Plasma membrane ATPase 2Add BLAST | 947 |
Proteomic databases
MaxQBi | P19657 |
PaxDbi | P19657 |
PRIDEi | P19657 |
PTM databases
iPTMneti | P19657 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 36143, 377 interactors |
DIPi | DIP-4036N |
IntActi | P19657, 4 interactors |
MINTi | P19657 |
STRINGi | 4932.YPL036W |
Miscellaneous databases
RNActi | P19657, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 33 – 47 | Poly-SerAdd BLAST | 15 | |
Compositional biasi | 62 – 91 | Asp/Glu-rich (acidic)Add BLAST | 30 |
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0205, Eukaryota |
GeneTreei | ENSGT00940000176570 |
HOGENOMi | CLU_002360_6_0_1 |
InParanoidi | P19657 |
OMAi | APLWVFK |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
P19657-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSTEAKQYK EKPSKEYLHA SDGDDPANNS AASSSSSSST STSASSSAAA
60 70 80 90 100
VPRKAAAASA ADDSDSDEDI DQLIDELQSN YGEGDESGEE EVRTDGVHAG
110 120 130 140 150
QRVVPEKDLS TDPAYGLTSD EVARRRKKYG LNQMAEENES LIVKFLMFFV
160 170 180 190 200
GPIQFVMEAA AILAAGLSDW VDVGVICALL LLNASVGFIQ EFQAGSIVDE
210 220 230 240 250
LKKTLANTAT VIRDGQLIEI PANEVVPGEI LQLESGTIAP ADGRIVTEDC
260 270 280 290 300
FLQIDQSAIT GESLAAEKHY GDEVFSSSTV KTGEAFMVVT ATGDNTFVGR
310 320 330 340 350
AAALVGQASG VEGHFTEVLN GIGIILLVLV IATLLLVWTA CFYRTVGIVS
360 370 380 390 400
ILRYTLGITI IGVPVGLPAV VTTTMAVGAA YLAKKQAIVQ KLSAIESLAG
410 420 430 440 450
VEILCSDKTG TLTKNKLSLH EPYTVEGVSP DDLMLTACLA ASRKKKGLDA
460 470 480 490 500
IDKAFLKSLI EYPKAKDALT KYKVLEFHPF DPVSKKVTAV VESPEGERIV
510 520 530 540 550
CVKGAPLFVL KTVEEDHPIP EDVHENYENK VAELASRGFR ALGVARKRGE
560 570 580 590 600
GHWEILGVMP CMDPPRDDTA QTINEARNLG LRIKMLTGDA VGIAKETCRQ
610 620 630 640 650
LGLGTNIYNA ERLGLGGGGD MPGSELADFV ENADGFAEVF PQHKYRVVEI
660 670 680 690 700
LQNRGYLVAM TGDGVNDAPS LKKADTGIAV EGATDAARSA ADIVFLAPGL
710 720 730 740 750
SAIIDALKTS RQIFHRMYSY VVYRIALSLH LEIFLGLWIA ILNNSLDINL
760 770 780 790 800
IVFIAIFADV ATLTIAYDNA PYAPEPVKWN LPRLWGMSII LGIVLAIGSW
810 820 830 840 850
ITLTTMFLPN GGIIQNFGAM NGVMFLQISL TENWLIFVTR AAGPFWSSIP
860 870 880 890 900
SWQLAGAVFA VDIIATMFTL FGWWSENWTD IVSVVRVWIW SIGIFCVLGG
910 920 930 940
FYYIMSTSQA FDRLMNGKSL KEKKSTRSVE DFMAAMQRVS TQHEKSS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 944 | E → D in AAA83387 (PubMed:2904437).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04421 Genomic DNA Translation: AAA83387.1 U44030 Genomic DNA Translation: AAB68184.1 BK006949 Genomic DNA Translation: DAA11394.1 |
PIRi | S62039, PXBY2P |
RefSeqi | NP_015289.1, NM_001183850.1 |
Genome annotation databases
EnsemblFungii | YPL036W_mRNA; YPL036W; YPL036W |
GeneIDi | 856071 |
KEGGi | sce:YPL036W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J04421 Genomic DNA Translation: AAA83387.1 U44030 Genomic DNA Translation: AAB68184.1 BK006949 Genomic DNA Translation: DAA11394.1 |
PIRi | S62039, PXBY2P |
RefSeqi | NP_015289.1, NM_001183850.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 36143, 377 interactors |
DIPi | DIP-4036N |
IntActi | P19657, 4 interactors |
MINTi | P19657 |
STRINGi | 4932.YPL036W |
PTM databases
iPTMneti | P19657 |
Proteomic databases
MaxQBi | P19657 |
PaxDbi | P19657 |
PRIDEi | P19657 |
Genome annotation databases
EnsemblFungii | YPL036W_mRNA; YPL036W; YPL036W |
GeneIDi | 856071 |
KEGGi | sce:YPL036W |
Organism-specific databases
EuPathDBi | FungiDB:YPL036W |
SGDi | S000005957, PMA2 |
Phylogenomic databases
eggNOGi | KOG0205, Eukaryota |
GeneTreei | ENSGT00940000176570 |
HOGENOMi | CLU_002360_6_0_1 |
InParanoidi | P19657 |
OMAi | APLWVFK |
Miscellaneous databases
PROi | PR:P19657 |
RNActi | P19657, protein |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA2_YEAST | |
Accessioni | P19657Primary (citable) accession number: P19657 Secondary accession number(s): D6W3X8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | October 1, 1996 | |
Last modified: | December 2, 2020 | |
This is version 190 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast chromosome XVI
Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD