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UniProtKB - P19636 (EUTC_ECOLI)

Entry version 149 (25 May 2022)
Sequence version 2 (01 Nov 1997)
Previous versions | rss
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Protein

Ethanolamine ammonia-lyase small subunit

Gene

eutC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the deamination of various vicinal amino-alcohols to oxo compounds (PubMed:19762342).

Ethanolamine ammonia-lyase (EAL) allows bacteria to utilize ethanolamine as the sole source of nitrogen and carbon in the presence of external vitamin B12. It is spontaneously inactivated by its substrate and reactivated by EutA (PubMed:15466038).

Directly targeted to the BMC. May play a role in bacterial microcompartment (BMC) assembly or maintenance (By similarity).

By similarity1 Publication2 Publications

Caution

In strain MG1655 the eut operon is interrupted by the CPZ-55 prophage, encoding 9 genes situated between eutA and eutB, which are translated in the other direction. CPZ-55 may prevent expression of the eut operon in strain MG1655. Strain W3110 does not have this prophage element and should be able to express the operon.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

adenosylcob(III)alaminUniRule annotation4 PublicationsNote: Binds 1 AdoCbl between the large and small subunits, with 6 cofactors per holoenzyme.UniRule annotation1 Publication3 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by 5-adeninylpentylcobalamin (AdePeCbl), a cofactor analog (PubMed:19762342). Irreversibly inhibited during catalysis by cleavage of the Co-C bond of the cobalamin coenzyme (Probable) (PubMed:19762342). Reactivated by EutA, which probably involves an ATP-dependent cobalamin exchange (PubMed:15466038).1 Publication2 Publications

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=8.2 µM for ethanolamine1 Publication
  2. KM=0.055 µM for adenosylcob(III)alamin1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: ethanolamine degradation

This protein is involved in the pathway ethanolamine degradation, which is part of Amine and polyamine degradation.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway ethanolamine degradation and in Amine and polyamine degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei207Adenosylcob(III)alamin; via amide nitrogenUniRule annotationCombined sources2 Publications1
Binding sitei228Adenosylcob(III)alaminUniRule annotationCombined sources2 Publications1
Binding sitei258Adenosylcob(III)alamin; via carbonyl oxygenUniRule annotationCombined sources2 Publications1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionLyase
LigandCobalamin, Cobalt

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EUTC-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		4.3.1.7, 2026

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00560

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Ethanolamine ammonia-lyase small subunit1 PublicationUniRule annotation (EC:4.3.1.7UniRule annotation2 Publications)
Short name:
EAL small subunit1 PublicationUniRule annotation
Alternative name(s):
Ethanolamine ammonia-lyase beta subunit1 Publication
Ethanolamine ammonia-lyase light chain
Ethanolamine deaminase small subunit1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:eutCUniRule annotation
Ordered Locus Names:b2440, JW2433
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Bacterial microcompartment

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002059881 – 295Ethanolamine ammonia-lyase small subunitAdd BLAST295

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P19636

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P19636

PRoteomics IDEntifications database

More...PRIDEi		P19636

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

When grown in ethanolamine and adenosylcobalamin (AdoCbl) (at protein level). Note this experiment was done in strain JM109, which expresses the eut operon.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

The basic unit is a heterodimer, which further associates into a trimer of heterotetramers (PubMed:20519496, PubMed:21142024, PubMed:29797764, PubMed:19762342). 6 large subunits form a core ring with 6 small subunits projecting outwards (Probable).

1 Publication4 Publications

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260577, 15 interactors
851264, 23 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-2313, Ethanolamine ammonia-lyase complex

Protein interaction database and analysis system

More...IntActi		P19636, 30 interactors

STRING: functional protein association networks

More...STRINGi		511145.b2440

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1295
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		P19636

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P19636

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P19636

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

Partial trypsin digestion of this subunit (removing the first 28 residues) yields an active protein less susceptible to aggregation.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the EutC family.UniRule annotationCurated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG4302, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_068224_0_0_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P19636

Identification of Orthologs from Complete Genome Data

More...OMAi		ADRNCVS

Database for complete collections of gene phylogenies

More...PhylomeDBi		P19636

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.50.11240, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_00601, EutC, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR009246, EutC
IPR042251, EutC_C

The PANTHER Classification System

More...PANTHERi		PTHR39330, PTHR39330, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF05985, EutC, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF018982, EutC, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P19636-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDQKQIEEIV RSVMASMGQA APAPSEAKCA TTNCAAPVTS ESCALDLGSA 
        60         70         80         90        100
EAKAWIGVEN PHRADVLTEL RRSTVARVCT GRAGPRPRTQ ALLRFLADHS 
       110        120        130        140        150
RSKDTVLKEV PEEWVKAQGL LEVRSEISDK NLYLTRPDMG RRLCAEAVEA 
       160        170        180        190        200
LKAQCVANPD VQVVISDGLS TDAITVNYEE ILPPLMAGLK QAGLKVGTPF 
       210        220        230        240        250
FVRYGRVKIE DQIGEILGAK VVILLVGERP GLGQSESLSC YAVYSPRMAT 
       260        270        280        290 
TVEADRTCIS NIHQGGTPPV EAAAVIVDLA KRMLEQKASG INMTR
Length:295
Mass (Da):31,782
Last modified:November 1, 1997 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iC7E87143B8E050E5
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75493.1
AP009048 Genomic DNA Translation: BAA16322.1

Protein sequence database of the Protein Information Resource

More...PIRi		G65018

NCBI Reference Sequences

More...RefSeqi		NP_416935.1, NC_000913.3
WP_000372316.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75493; AAC75493; b2440
BAA16322; BAA16322; BAA16322

Database of genes from NCBI RefSeq genomes

More...GeneIDi		946925

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2433
eco:b2440

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.4291

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC75493.1
AP009048 Genomic DNA Translation: BAA16322.1
PIRiG65018
RefSeqiNP_416935.1, NC_000913.3
WP_000372316.1, NZ_LN832404.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ABOX-ray2.10B/D44-295[»]
3ABQX-ray2.05B/D44-295[»]
3ABRX-ray2.10B/D44-295[»]
3ABSX-ray2.25B/D44-295[»]
3ANYX-ray2.10B/D44-295[»]
3AO0X-ray2.25B/D44-295[»]
5YSNX-ray2.00B/D44-295[»]
5YSRX-ray2.05B/D44-295[»]
AlphaFoldDBiP19636
SMRiP19636
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4260577, 15 interactors
851264, 23 interactors
ComplexPortaliCPX-2313, Ethanolamine ammonia-lyase complex
IntActiP19636, 30 interactors
STRINGi511145.b2440

Proteomic databases

jPOSTiP19636
PaxDbiP19636
PRIDEiP19636

Genome annotation databases

EnsemblBacteriaiAAC75493; AAC75493; b2440
BAA16322; BAA16322; BAA16322
GeneIDi946925
KEGGiecj:JW2433
eco:b2440
PATRICifig|1411691.4.peg.4291

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB4300

Phylogenomic databases

eggNOGiCOG4302, Bacteria
HOGENOMiCLU_068224_0_0_6
InParanoidiP19636
OMAiADRNCVS
PhylomeDBiP19636

Enzyme and pathway databases

UniPathwayiUPA00560
BioCyciEcoCyc:EUTC-MONOMER
BRENDAi4.3.1.7, 2026

Miscellaneous databases

EvolutionaryTraceiP19636

Protein Ontology

More...PROi		PR:P19636

Family and domain databases

Gene3Di3.40.50.11240, 1 hit
HAMAPiMF_00601, EutC, 1 hit
InterProiView protein in InterPro
IPR009246, EutC
IPR042251, EutC_C
PANTHERiPTHR39330, PTHR39330, 1 hit
PfamiView protein in Pfam
PF05985, EutC, 1 hit
PIRSFiPIRSF018982, EutC, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEUTC_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P19636
Secondary accession number(s): P78273
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: November 1, 1997
Last modified: May 25, 2022
This is version 149 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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