UniProtKB - P19618 (NCPR_SALTR)
Protein
NADPH--cytochrome P450 reductase
Gene
por
Organism
Salmo trutta (Brown trout)
Status
Functioni
This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation
Catalytic activityi
- EC:1.6.2.4UniRule annotation
Cofactori
Protein has several cofactor binding sites:- FADUniRule annotationNote: Binds 1 FAD per monomer.UniRule annotation
- FMNUniRule annotationNote: Binds 1 FMN per monomer.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 153 | FMNUniRule annotation | 1 | |
Binding sitei | 423 | FADUniRule annotation | 1 | |
Binding sitei | 458 | NADPUniRule annotation | 1 | |
Binding sitei | 562 | NADPUniRule annotation | 1 | |
Binding sitei | 600 | FADUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 31 – 36 | FMNUniRule annotation | 6 | |
Nucleotide bindingi | 83 – 86 | FMNUniRule annotation | 4 | |
Nucleotide bindingi | 118 – 127 | FMNUniRule annotation | 10 | |
Nucleotide bindingi | 399 – 402 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 417 – 419 | FADUniRule annotation | 3 | |
Nucleotide bindingi | 427 – 430 | FADUniRule annotation | 4 | |
Nucleotide bindingi | 519 – 520 | NADPUniRule annotation | 2 | |
Nucleotide bindingi | 525 – 529 | NADPUniRule annotation | 5 |
GO - Molecular functioni
- FMN binding Source: InterPro
- NADPH-hemoprotein reductase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Oxidoreductase |
Ligand | FAD, Flavoprotein, FMN, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)Short name: CPRUniRule annotation Short name: P450RUniRule annotation |
Gene namesi | Name:porUniRule annotation |
Organismi | Salmo trutta (Brown trout) |
Taxonomic identifieri | 8032 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Salmo |
Proteomesi |
|
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane UniRule annotation; Single-pass membrane protein UniRule annotation; Cytoplasmic side UniRule annotation
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000167601 | ‹1 – 601 | NADPH--cytochrome P450 reductaseAdd BLAST | ›601 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 169 | Flavodoxin-likeUniRule annotationAdd BLAST | 145 | |
Domaini | 224 – 425 | FAD-binding FR-typeUniRule annotationAdd BLAST | 202 |
Sequence similaritiesi
Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
i Sequence
Sequence statusi: Fragments.
P19618-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
KLDQPAPSTQ ETSFIEKMKK TGRNIVVFYG SQTGTGEEFA NRLSKDAHRY
60 70 80 90 100
GMGSMAADPE EYDMSELSRL AEIGNSLAIF CMATYGEGDP TDNAQDFYDW
110 120 130 140 150
LQETDGQLSG VNYPVFALGD KTYEHYNAMG AYVDKRLEEL GAKRVFDLGM
160 170 180 190 200
GDDDGNLEED FVTWREQFWP AMCEHFGVEA SGEDSSVRQY ELKEHNDINM
210 220 230 240 250
NKVYTGELGR LKSFETQKPP FDAKNPFLAP VTVNRKLNKA GELHKMHLEV
260 270 280 290 300
DITGSKIRYE SGDHVAVYPT NNTVIVNRLG QILGVDLDSV ISLNNLDEES
310 320 330 340 350
NKKHPFPCPT TYRTALTHYL DIIHPPRTNV LYELAQYATD LKDQENTDSM
360 370 380 390 400
ASSAPEGKAL YQSFVLEDNR NILAILEDLP SLRPPIDHLC ELMPRLQARY
410 420 430 440 450
YSIASSSKVH PNSIHICAVL VEYXTKGVAT TWLKYIRKSQ FRLPFKASNP
460 470 480 490 500
VIMVGPGTGI APFMGFIQER GWLKESGKEV GETVLYCGCR HKEEDYLYQE
510 520 530 540 550
ELEQAHKKGA LTKLNVAFSR EQDQKVYVQH LLRKNKVDLW RQIHEDYAHI
560 570 580 590 600
YICGDARNMA RDVQTAFYEI AEELGGMTRT QATDYIKKLM TKGRYSQDVW
S
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 | ||
Non-adjacent residuesi | 426 – 427 | Curated | 2 | |
Non-adjacent residuesi | 434 – 435 | Curated | 2 |
Sequence databases
PIRi | A28577 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | A28577 |
3D structure databases
ModBasei | Search... |
Family and domain databases
Gene3Di | 1.20.990.10, 2 hits 3.40.50.360, 1 hit 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SUPFAMi | SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCPR_SALTR | |
Accessioni | P19618Primary (citable) accession number: P19618 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | February 1, 1991 | |
Last modified: | December 2, 2020 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families