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Entry version 105 (08 May 2019)
Sequence version 3 (01 Oct 1996)
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Protein

Pyruvate-flavodoxin oxidoreductase

Gene

nifJ

Organism
Enterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Oxidoreductase required for the transfer of electrons from pyruvate to flavodoxin, which reduces nitrogenase.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi690Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi693Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi696Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi700Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi744Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi747Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi750Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi754Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi810Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi813Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi838Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi1075Iron-sulfur 3 (4Fe-4S)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrogen fixation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Pyruvate-flavodoxin oxidoreductase (EC:1.2.7.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nifJ
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates if the gene coding for the protein originates from the hydrogenosome, the mitochondrion, the nucleomorph, different plastids or a plasmid. The absence of this section means that the gene is located in one of the main chromosomal element(s).<p><a href='/help/encoded_on' target='_top'>More...</a></p>Encoded oniPlasmid pEA30 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEnterobacter agglomerans (Erwinia herbicola) (Pantoea agglomerans)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri549 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaePantoeaPantoea agglomerans group

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002155541 – 1173Pyruvate-flavodoxin oxidoreductaseAdd BLAST1173

Proteomic databases

PRoteomics IDEntifications database

More...
PRIDEi
P19543

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P19543

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini681 – 7104Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini735 – 7664Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST32

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Repeat

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.40.50.920, 1 hit
3.40.920.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR033412 PFOR_II
IPR019456 Pyrv-flavodox_OxRtase_EKR
IPR019752 Pyrv/ketoisovalerate_OxRed_cat
IPR002880 Pyrv_Fd/Flavodoxin_OxRdtase_N
IPR011895 Pyrv_flavodox_OxRed
IPR002869 Pyrv_flavodox_OxRed_cen
IPR029061 THDP-binding
IPR011766 TPP_enzyme-bd_C
IPR009014 Transketo_C/PFOR_II

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF10371 EKR, 1 hit
PF17147 PFOR_II, 1 hit
PF01558 POR, 1 hit
PF01855 POR_N, 1 hit
PF02775 TPP_enzyme_C, 1 hit

PIRSF; a whole-protein classification database

More...
PIRSFi
PIRSF000159 NifJ, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00890 EKR, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52518 SSF52518, 2 hits
SSF52922 SSF52922, 1 hit
SSF53323 SSF53323, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02176 pyruv_ox_red, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P19543-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGKMKTMDG NTAAAYVSYA FTDVTAIYPI TPSTPMAESV DEWAAQGKKN
60 70 80 90 100
LFGQTVKIME MQSESGAAGA IHGALQAGAL ATTYTASQGL LLMTPNMYKI
110 120 130 140 150
AGELLPAVFH VSARALATNS LNIFGDHQDS YAVRHTGCAM LAESSVQQVM
160 170 180 190 200
DLAAVAHLTA IKGRIPFINF FDGFRTSHEI QKIEVLDYED LAHLLDRDAV
210 220 230 240 250
ARFRQNALHP DHPVVRGTAQ NPDIYFQERE ASNKFYMALP EMVESYMAKI
260 270 280 290 300
SALTGREYHL FNYYGAPDAG RMIIAMGSVC ETIQETVDYL NARGEKVGVL
310 320 330 340 350
TVHLYRPFSL KHFFKYIPKS VSNIAVLDRT KEPGSLAEPL YLDVKSAFYN
360 370 380 390 400
SDWRPVIVGG RYALGGKDIL PSHIISIFDN LAAERPRDGF TVGINDDVTF
410 420 430 440 450
TSLPLSDRDI DTSSSGTTAC KFWGLGSDGT VGANKSAIKI IGDKTDMYAQ
460 470 480 490 500
AYFAYDSKKS GGVTMSHLRF GHNPIRSPYL IDKADFISCS QQSYVNKYHL
510 520 530 540 550
LAGLKPGGTF LLNCSWDVAE LDEKLPVAMK RYIAANDIQF YIVNAVGIAQ
560 570 580 590 600
KLGLGGRFNM IMQSAFFKLA DIIPLTRAVE YLKQSIEHAY GNKGQKIVDM
610 620 630 640 650
NNLAVDSGIE SVVKISVPQA WKHLEDKVVS PKKLPAFIKD ILIPMNRQEG
660 670 680 690 700
DSLPVSIFDG IEDGTFPSGT SAYEKRGVAI NVPVWQTDKC TQCNQCAFIC
710 720 730 740 750
PHAAIRPVLI SEEERQNAPA GFSAKRASGT EDAWYRLAVS PLDCSGCGNC
760 770 780 790 800
ADVCPVKGKA LSMQPLESQE HEIELWEYAL SLTPKANPQN KFTVKGSQFE
810 820 830 840 850
QPLLEFSGAC GGCGETPYAK LVTQLFGDRM MIANATGCSS IWGASAPSIP
860 870 880 890 900
YTTNHKGQGP TWANSLFEDN AEFGLGMLLG VDAIRDTLAT QVKAALDNAP
910 920 930 940 950
DVPLDAELSA CLSDWLANKD QGEGTRERAE KVGDTSGFAN AREKSRTPTV
960 970 980 990 1000
SMPHRDYLAN GSHWIFGGDG WAYDIGFGGL DHVLASGKDV NVLVFDTEVY
1010 1020 1030 1040 1050
SNTGGQSSKS TPAAAIAQFA CKKTRKKDLG MMAMSYGYVY VAQIAMGADK
1060 1070 1080 1090 1100
NQTLRAIAEA EAYPGPSLII AYAACINHGL RIGMGCSQRE ARRAVEAGYW
1110 1120 1130 1140 1150
ANYRYHPELK EAGKNPFILD SEEPEEDFQA FLAGEVRYSS LKKLYPEFAE
1160 1170
FLFKKTEDDA RERLEGYKKL ASS
Length:1,173
Mass (Da):128,246
Last modified:October 1, 1996 - v3
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0B35809565C5DE4B
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X78558 Genomic DNA Translation: CAA55302.1
X99694 Genomic DNA Translation: CAA68011.1
M26931 Genomic DNA Translation: AAA24806.2
M38221 Genomic DNA Translation: AAA23386.1

Protein sequence database of the Protein Information Resource

More...
PIRi
B39414

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78558 Genomic DNA Translation: CAA55302.1
X99694 Genomic DNA Translation: CAA68011.1
M26931 Genomic DNA Translation: AAA24806.2
M38221 Genomic DNA Translation: AAA23386.1
PIRiB39414

3D structure databases

SMRiP19543
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP19543

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.920, 1 hit
3.40.920.10, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR033412 PFOR_II
IPR019456 Pyrv-flavodox_OxRtase_EKR
IPR019752 Pyrv/ketoisovalerate_OxRed_cat
IPR002880 Pyrv_Fd/Flavodoxin_OxRdtase_N
IPR011895 Pyrv_flavodox_OxRed
IPR002869 Pyrv_flavodox_OxRed_cen
IPR029061 THDP-binding
IPR011766 TPP_enzyme-bd_C
IPR009014 Transketo_C/PFOR_II
PfamiView protein in Pfam
PF10371 EKR, 1 hit
PF17147 PFOR_II, 1 hit
PF01558 POR, 1 hit
PF01855 POR_N, 1 hit
PF02775 TPP_enzyme_C, 1 hit
PIRSFiPIRSF000159 NifJ, 1 hit
SMARTiView protein in SMART
SM00890 EKR, 1 hit
SUPFAMiSSF52518 SSF52518, 2 hits
SSF52922 SSF52922, 1 hit
SSF53323 SSF53323, 1 hit
TIGRFAMsiTIGR02176 pyruv_ox_red, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNIFJ_ENTAG
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P19543
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: October 1, 1996
Last modified: May 8, 2019
This is version 105 of the entry and version 3 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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