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Protein

Protein Vpx

Gene

vpx

Organism
Simian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39Zinc; via pros nitrogen1 Publication1
Metal bindingi82Zinc; via tele nitrogen1 Publication1
Metal bindingi87Zinc1 Publication1
Metal bindingi89Zinc1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Inhibition of host innate immune response by virus, Viral immunoevasion
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Vpx
Alternative name(s):
Viral protein X
X ORF protein
Gene namesi
Name:vpx
OrganismiSimian immunodeficiency virus (isolate PBj14/BCL-3) (SIV-sm) (Simian immunodeficiency virus sooty mangabey monkey)
Taxonomic identifieri11738 [NCBI]
Taxonomic lineageiVirusesOrterviralesRetroviridaeOrthoretrovirinaeLentivirus
Virus hostiCercopithecidae (Old World monkeys) [TaxID: 9527]
Proteomesi
  • UP000007221 Componenti: Genome

Subcellular locationi

  • Virion
  • Host nucleus
  • Note: Nuclear just after virion uncoating, or if expressed in the absence of unprocessed GAG.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000854071 – 112Protein VpxAdd BLAST112

Interactioni

Subunit structurei

Interacts with the P6 region of unprocessed GAG. Interacts with host VPRBP/DCAF1, leading to change substrate specificity of the CUL4A-DDB1 E3 ligase complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DCAF1Q9Y4B64EBI-6558117,EBI-1996353From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-60689N
IntActiP19508, 1 interactor

Structurei

Secondary structure

1112
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 15Combined sources3
Helixi18 – 36Combined sources19
Turni37 – 39Combined sources3
Helixi42 – 56Combined sources15
Turni57 – 60Combined sources4
Helixi64 – 84Combined sources21

3D structure databases

ProteinModelPortaliP19508
SMRiP19508
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi65 – 72Nuclear localization signalBy similarity8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi101 – 109Poly-Pro9

Sequence similaritiesi

Belongs to the lentivirus VPX protein family.Curated

Phylogenomic databases

OrthoDBiVOG090001GG

Family and domain databases

InterProiView protein in InterPro
IPR000012 RetroV_VpR/X
PfamiView protein in Pfam
PF00522 VPR, 1 hit

Sequencei

Sequence statusi: Complete.

P19508-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDPRERIPP GNSGEETIGE AFDWLDRTVE EINRAAVNHL PRELIFQVWR
60 70 80 90 100
RSWEYWHDEM GMSVSYTKYR YLCLIQKAMF MHCKKGCRCL GGEHGAGGWR
110
PGPPPPPPPG LA
Length:112
Mass (Da):12,855
Last modified:February 1, 1991 - v1
Checksum:i226D0BF3A0FD2713
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03298 Genomic RNA Translation: AAA47779.1
M31325 Genomic RNA Translation: AAA47755.1

Similar proteinsi

Entry informationi

Entry nameiVPX_SIVSP
AccessioniPrimary (citable) accession number: P19508
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1991
Last sequence update: February 1, 1991
Last modified: June 20, 2018
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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