UniProtKB - P19456 (PMA2_ARATH)
ATPase 2, plasma membrane-type
AHA2
Functioni
Miscellaneous
Catalytic activityi
- EC:7.1.2.1Curated
Activity regulationi
Kineticsi
- KM=1.2 mM for ATP1 Publication
- Vmax=0.87 µmol/min/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 329 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 588 | MagnesiumBy similarity | 1 | |
Metal bindingi | 592 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- magnesium ion binding Source: UniProtKB
- proton-exporting ATPase activity, phosphorylative mechanism Source: UniProtKB
GO - Biological processi
- proton export across plasma membrane Source: InterPro
- proton transmembrane transport Source: UniProtKB
- regulation of intracellular pH Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Hydrogen ion transport, Ion transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT4G30190-MONOMER MetaCyc:AT4G30190-MONOMER |
BRENDAi | 3.6.3.6, 399 |
SABIO-RKi | P19456 |
Protein family/group databases
TCDBi | 3.A.3.3.9, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: ATPase 2, plasma membrane-type1 Publication (EC:7.1.2.1Curated)Alternative name(s): Proton pump 2 |
Gene namesi | Name:AHA21 Publication Ordered Locus Names:At4g30190Imported ORF Names:F9N11.40Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G30190 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication3 Publications; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 61 | CytoplasmicSequence analysisAdd BLAST | 60 | |
Transmembranei | 62 – 81 | Helical; Name=1Sequence analysisAdd BLAST | 20 | |
Topological domaini | 82 – 93 | ExtracellularSequence analysisAdd BLAST | 12 | |
Transmembranei | 94 – 114 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 115 – 243 | CytoplasmicSequence analysisAdd BLAST | 129 | |
Transmembranei | 244 – 264 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 265 – 273 | ExtracellularSequence analysis | 9 | |
Transmembranei | 274 – 291 | Helical; Name=4Sequence analysisAdd BLAST | 18 | |
Topological domaini | 292 – 643 | CytoplasmicSequence analysisAdd BLAST | 352 | |
Transmembranei | 644 – 665 | Helical; Name=5Sequence analysisAdd BLAST | 22 | |
Topological domaini | 666 – 670 | ExtracellularSequence analysis | 5 | |
Transmembranei | 671 – 693 | Helical; Name=6Sequence analysisAdd BLAST | 23 | |
Topological domaini | 694 – 709 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 710 – 730 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 731 – 751 | ExtracellularSequence analysisAdd BLAST | 21 | |
Transmembranei | 752 – 772 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 773 – 784 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 785 – 805 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 806 – 813 | ExtracellularSequence analysis | 8 | |
Transmembranei | 814 – 834 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 835 – 948 | CytoplasmicSequence analysisAdd BLAST | 114 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 106 | N → A, D, K, Q or T: Reduced proton transport. 1 Publication | 1 | |
Mutagenesisi | 655 | R → A or D: No effect on ATP affinity, but reduced proton transport. 1 Publication | 1 | |
Mutagenesisi | 655 | R → K: No effect on ATP affinity and proton transport. 1 Publication | 1 | |
Mutagenesisi | 684 | D → A, V or R: No effect on ATP affinity, but loss of proton transport. 1 Publication | 1 | |
Mutagenesisi | 684 | D → E: No effect on ATP affinity, but reduced proton transport. 1 Publication | 1 | |
Mutagenesisi | 684 | D → N: Insensitive to the inhibitor vanadate and locked in the E1 conformation. No effect on ATP hydrolysis, but loss of proton transport. 2 Publications | 1 | |
Mutagenesisi | 881 | T → A: Decreased phosphorylation by PSY1R. 1 Publication | 1 | |
Mutagenesisi | 881 | T → D: No effect on 14-3-3 protein binding, but increased activity. 1 Publication | 1 | |
Mutagenesisi | 904 | S → A: No effect on phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 919 | L → A: Loss of activation by lysophospholipids. 1 Publication | 1 | |
Mutagenesisi | 922 | L → A: Increased activation by lysophospholipids. 1 Publication | 1 | |
Mutagenesisi | 924 | T → A: No effect on phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 931 | S → A: Loss of phosphorylation and increased 14-3-3 protein binding. 1 Publication | 1 | |
Mutagenesisi | 931 | S → D: Loss of interaction with 14-3-3 protein. 1 Publication | 1 | |
Mutagenesisi | 942 | T → A: No effect on phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 947 | T → A: No effect on phosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000046275 | 2 – 948 | ATPase 2, plasma membrane-typeAdd BLAST | 947 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 881 | Phosphothreonine1 Publication | 1 | |
Modified residuei | 899 | Phosphoserine1 Publication | 1 | |
Modified residuei | 931 | Phosphoserine; by CIPK111 Publication | 1 | |
Modified residuei | 947 | Phosphothreonine1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | P19456 |
PRIDEi | P19456 |
ProteomicsDBi | 236644 [P19456-1] |
PTM databases
iPTMneti | P19456 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
ExpressionAtlasi | P19456, baseline and differential |
Genevisiblei | P19456, AT |
Interactioni
Subunit structurei
Binds to 14-3-3 proteins (PubMed:10593986). The binding is induced by phosphorylation of Thr-947 and it activates the H+-ATPase (PubMed:10593986).
Interacts (via the R-domain) with PSY1R (via C-terminus) (PubMed:25267325).
Part of a functional complex containing PSKR1, BAK1, CNGC17, and AHA (PubMed:26071421).
Interacts with CNGC17 and PSKR1 (PubMed:26071421).
3 PublicationsBinary interactionsi
P19456
With | #Exp. | IntAct |
---|---|---|
CIPK11 [O22932] | 3 | EBI-2293350,EBI-537638 |
Protein-protein interaction databases
BioGRIDi | 14429, 43 interactors |
IntActi | P19456, 30 interactors |
STRINGi | 3702.AT4G30190.2 |
Structurei
Secondary structure
3D structure databases
SMRi | P19456 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P19456 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 946 – 948 | Interaction with 14-3-3 proteins | 3 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_4_1 |
InParanoidi | P19456 |
PhylomeDBi | P19456 |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketThis entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSSLEDIKNE TVDLEKIPIE EVFQQLKCSR EGLTTQEGED RIQIFGPNKL
60 70 80 90 100
EEKKESKLLK FLGFMWNPLS WVMEMAAIMA IALANGDGRP PDWQDFVGII
110 120 130 140 150
CLLVINSTIS FIEENNAGNA AAALMAGLAP KTKVLRDGKW SEQEAAILVP
160 170 180 190 200
GDIVSIKLGD IIPADARLLE GDPLKVDQSA LTGESLPVTK HPGQEVFSGS
210 220 230 240 250
TCKQGEIEAV VIATGVHTFF GKAAHLVDST NQVGHFQKVL TAIGNFCICS
260 270 280 290 300
IAIGMVIEII VMYPIQRRKY RDGIDNLLVL LIGGIPIAMP TVLSVTMAIG
310 320 330 340 350
SHRLSQQGAI TKRMTAIEEM AGMDVLCSDK TGTLTLNKLS VDKNLVEVFC
360 370 380 390 400
KGVEKDQVLL FAAMASRVEN QDAIDAAMVG MLADPKEARA GIREVHFLPF
410 420 430 440 450
NPVDKRTALT YIDGSGNWHR VSKGAPEQIL ELAKASNDLS KKVLSIIDKY
460 470 480 490 500
AERGLRSLAV ARQVVPEKTK ESPGAPWEFV GLLPLFDPPR HDSAETIRRA
510 520 530 540 550
LNLGVNVKMI TGDQLAIGKE TGRRLGMGTN MYPSSALLGT HKDANLASIP
560 570 580 590 600
VEELIEKADG FAGVFPEHKY EIVKKLQERK HIVGMTGDGV NDAPALKKAD
610 620 630 640 650
IGIAVADATD AARGASDIVL TEPGLSVIIS AVLTSRAIFQ RMKNYTIYAV
660 670 680 690 700
SITIRIVFGF MLIALIWEFD FSAFMVLIIA ILNDGTIMTI SKDRVKPSPT
710 720 730 740 750
PDSWKLKEIF ATGVVLGGYQ AIMTVIFFWA AHKTDFFSDT FGVRSIRDNN
760 770 780 790 800
HELMGAVYLQ VSIISQALIF VTRSRSWSFV ERPGALLMIA FLIAQLIATL
810 820 830 840 850
IAVYANWEFA KIRGIGWGWA GVIWLYSIVT YFPLDVFKFA IRYILSGKAW
860 870 880 890 900
LNLFENKTAF TMKKDYGKEE REAQWALAQR TLHGLQPKEA VNIFPEKGSY
910 920 930 940
RELSEIAEQA KRRAEIARLR ELHTLKGHVE SVVKLKGLDI ETPSHYTV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4JPJ7 | F4JPJ7_ARATH | Plasma membrane ATPase | HA2 AHA2, H(+)-ATPase 2, P-TYPE H(+)-ATPASE ISOFORM 2, PMA2, At4g30190 | 981 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05570 Genomic DNA Translation: AAA32751.1 AL109796 Genomic DNA Translation: CAB52463.1 AL161576 Genomic DNA Translation: CAB81012.1 CP002687 Genomic DNA Translation: AEE85731.1 AY035075 mRNA Translation: AAK59580.1 BT000781 mRNA Translation: AAN31920.1 BT001969 mRNA Translation: AAN71968.1 |
PIRi | A37116, PXMUP2 |
RefSeqi | NP_194748.1, NM_119165.4 [P19456-1] |
Genome annotation databases
EnsemblPlantsi | AT4G30190.1; AT4G30190.1; AT4G30190 [P19456-1] |
GeneIDi | 829142 |
Gramenei | AT4G30190.1; AT4G30190.1; AT4G30190 [P19456-1] |
KEGGi | ath:AT4G30190 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05570 Genomic DNA Translation: AAA32751.1 AL109796 Genomic DNA Translation: CAB52463.1 AL161576 Genomic DNA Translation: CAB81012.1 CP002687 Genomic DNA Translation: AEE85731.1 AY035075 mRNA Translation: AAK59580.1 BT000781 mRNA Translation: AAN31920.1 BT001969 mRNA Translation: AAN71968.1 |
PIRi | A37116, PXMUP2 |
RefSeqi | NP_194748.1, NM_119165.4 [P19456-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5KSD | X-ray | 3.50 | A/B | 12-844 | [»] | |
SMRi | P19456 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 14429, 43 interactors |
IntActi | P19456, 30 interactors |
STRINGi | 3702.AT4G30190.2 |
Protein family/group databases
TCDBi | 3.A.3.3.9, the p-type atpase (p-atpase) superfamily |
PTM databases
iPTMneti | P19456 |
Proteomic databases
PaxDbi | P19456 |
PRIDEi | P19456 |
ProteomicsDBi | 236644 [P19456-1] |
Genome annotation databases
EnsemblPlantsi | AT4G30190.1; AT4G30190.1; AT4G30190 [P19456-1] |
GeneIDi | 829142 |
Gramenei | AT4G30190.1; AT4G30190.1; AT4G30190 [P19456-1] |
KEGGi | ath:AT4G30190 |
Organism-specific databases
Araporti | AT4G30190 |
Phylogenomic databases
eggNOGi | KOG0205, Eukaryota |
HOGENOMi | CLU_002360_6_4_1 |
InParanoidi | P19456 |
PhylomeDBi | P19456 |
Enzyme and pathway databases
BioCyci | ARA:AT4G30190-MONOMER MetaCyc:AT4G30190-MONOMER |
BRENDAi | 3.6.3.6, 399 |
SABIO-RKi | P19456 |
Miscellaneous databases
EvolutionaryTracei | P19456 |
PROi | PR:P19456 |
Gene expression databases
ExpressionAtlasi | P19456, baseline and differential |
Genevisiblei | P19456, AT |
Family and domain databases
CDDi | cd02076, P-type_ATPase_H, 1 hit |
Gene3Di | 3.40.1110.10, 1 hit |
InterProi | View protein in InterPro IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR006534, P-type_ATPase_IIIA IPR001757, P_typ_ATPase |
Pfami | View protein in Pfam PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01647, ATPase-IIIA_H, 1 hit TIGR01494, ATPase_P-type, 2 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMA2_ARATH | |
Accessioni | P19456Primary (citable) accession number: P19456 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families