UniProtKB - P19426 (NELFE_MOUSE)
Protein
Negative elongation factor E
Gene
Nelfe
Organism
Mus musculus (Mouse)
Status
Functioni
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (By similarity). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (By similarity). Provides the strongest RNA binding activity of the NELF complex and may initially recruit the NELF complex to RNA (By similarity).By similarity
GO - Molecular functioni
- chromatin binding Source: Ensembl
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- negative regulation of mRNA polyadenylation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: Ensembl
- negative regulation of transcription elongation from RNA polymerase II promoter Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: Ensembl
- positive regulation of histone H3-K4 methylation Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: Ensembl
Keywordsi
Molecular function | Repressor, RNA-binding |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-75955, RNA Polymerase II Transcription Elongation |
Names & Taxonomyi
Protein namesi | Recommended name: Negative elongation factor EShort name: NELF-E Alternative name(s): RNA-binding protein RD |
Gene namesi | Name:Nelfe Synonyms:D17h6s45, Rd, Rdbp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:102744, Nelfe |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- NELF complex Source: UniProtKB
- nuclear body Source: MGI
- nucleoplasm Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081803 | 1 – 375 | Negative elongation factor EAdd BLAST | 375 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | PhosphoserineBy similarity | 1 | |
Cross-linki | 78 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity | ||
Cross-linki | 78 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity | ||
Cross-linki | 82 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 113 | PhosphoserineBy similarity | 1 | |
Modified residuei | 115 | PhosphoserineCombined sources | 1 | |
Modified residuei | 131 | PhosphoserineCombined sources | 1 | |
Modified residuei | 139 | PhosphoserineBy similarity | 1 | |
Modified residuei | 179 | PhosphoserineBy similarity | 1 | |
Modified residuei | 181 | PhosphoserineBy similarity | 1 | |
Modified residuei | 185 | PhosphoserineBy similarity | 1 | |
Modified residuei | 253 | PhosphoserineBy similarity | 1 | |
Modified residuei | 255 | PhosphoserineBy similarity | 1 | |
Modified residuei | 276 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 278 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 285 | PhosphoserineBy similarity | 1 | |
Modified residuei | 357 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Sumoylated.By similarity
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P19426 |
MaxQBi | P19426 |
PaxDbi | P19426 |
PeptideAtlasi | P19426 |
PRIDEi | P19426 |
PTM databases
iPTMneti | P19426 |
PhosphoSitePlusi | P19426 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024369, Expressed in testis and 302 other tissues |
ExpressionAtlasi | P19426, baseline and differential |
Genevisiblei | P19426, MM |
Interactioni
Subunit structurei
The NELF complex is composed of NELFA, NELFB, NELFCD and NELFE (By similarity).
Interacts with NELFB (PubMed:26010750).
By similarity1 PublicationBinary interactionsi
P19426
With | #Exp. | IntAct |
---|---|---|
Hsp90ab1 [P11499] | 2 | EBI-6142845,EBI-492813 |
Protein-protein interaction databases
BioGRIDi | 205393, 6 interactors |
IntActi | P19426, 1 interactor |
STRINGi | 10090.ENSMUSP00000134272 |
Miscellaneous databases
RNActi | P19426, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 184 – 185 | 1 | 2 | |
Repeati | 186 – 187 | 2 | 2 | |
Repeati | 188 – 189 | 3 | 2 | |
Repeati | 190 – 191 | 4 | 2 | |
Repeati | 192 – 193 | 5; approximate | 2 | |
Repeati | 194 – 195 | 6 | 2 | |
Repeati | 196 – 197 | 7 | 2 | |
Repeati | 198 – 199 | 8 | 2 | |
Repeati | 200 – 201 | 9 | 2 | |
Repeati | 202 – 203 | 10; approximate | 2 | |
Repeati | 204 – 205 | 11 | 2 | |
Repeati | 206 – 207 | 12 | 2 | |
Repeati | 208 – 209 | 13 | 2 | |
Repeati | 210 – 211 | 14 | 2 | |
Repeati | 212 – 213 | 15 | 2 | |
Repeati | 214 – 215 | 16 | 2 | |
Repeati | 216 – 217 | 17 | 2 | |
Repeati | 218 – 219 | 18; approximate | 2 | |
Repeati | 220 – 221 | 19; approximate | 2 | |
Repeati | 222 – 223 | 20 | 2 | |
Repeati | 224 – 225 | 21 | 2 | |
Repeati | 226 – 227 | 22 | 2 | |
Repeati | 228 – 229 | 23 | 2 | |
Repeati | 230 – 231 | 24; approximate | 2 | |
Repeati | 232 – 233 | 25 | 2 | |
Repeati | 234 – 235 | 26 | 2 | |
Repeati | 236 – 237 | 27 | 2 | |
Repeati | 238 – 239 | 28 | 2 | |
Repeati | 240 – 241 | 29 | 2 | |
Repeati | 242 – 243 | 30 | 2 | |
Repeati | 244 – 245 | 31 | 2 | |
Repeati | 246 – 247 | 32 | 2 | |
Domaini | 266 – 336 | RRMPROSITE-ProRule annotationAdd BLAST | 71 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 184 – 247 | 32 X 2 AA approximate tandem repeats of R-[DSE]Add BLAST | 64 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 7 – 36 | Sequence analysisAdd BLAST | 30 |
Domaini
The RRM domain interacts with RNA, and is essential for NELF complex function. It is however not required for the NELF complex formation (By similarity).By similarity
Sequence similaritiesi
Belongs to the RRM NELF-E family.Curated
Keywords - Domaini
Coiled coil, RepeatPhylogenomic databases
eggNOGi | ENOG502QQQ4, Eukaryota |
GeneTreei | ENSGT00630000089917 |
HOGENOMi | CLU_055643_0_0_1 |
InParanoidi | P19426 |
OMAi | RRQPIIE |
PhylomeDBi | P19426 |
TreeFami | TF324087 |
Family and domain databases
CDDi | cd12305, RRM_NELFE, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR033102, NELFE IPR034637, NELFE_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR17250, PTHR17250, 2 hits |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P19426-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSGP ASQGGVKRSL
60 70 80 90 100
SEQPVVDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE
110 120 130 140 150
KGPVPTFQPF QRSMSADEDL QEPSRRPQRK SLYESFVSSS DRLRELGQDG
160 170 180 190 200
EEAEAPGAGD GPPRGFDWSY EEHGSARSSA SPPRSRSRDR SHDRSRDRDR
210 220 230 240 250
DKERDRDRDR DRDRDRDKDK DRDRDRDRDK ERDRDRDRDR DRERDREGPF
260 270 280 290 300
RRSDSFPERR APRKGNTLYV YGEDMTPTLL RGAFSPFGNI IDLSMDPPRN
310 320 330 340 350
CAFVTYEKME SADQAVAELN GTQVESVQLK VNIARKQPML DAATGKSVWG
360 370
SLAVQNSPKG CHRDKRTQIV YSDDL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketG3UY39 | G3UY39_MOUSE | Negative elongation factor E | Nelfe Rdbp | 228 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 18 | K → E in AAA39680 (PubMed:3353717).Curated | 1 |
Polymorphismi
The length of the RD repeats is variable.1 Publication
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 228 – 229 | Missing in strain: LP.RIII/Sn. 1 Publication | 2 | |
Natural varianti | 229 – 236 | Missing in strain: 129/Sv. 1 Publication | 8 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008960 | 224 – 231 | Missing in isoform 2. 2 Publications | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21332 Genomic DNA Translation: AAA39680.1 AK012389 mRNA Translation: BAB28206.1 BC034867 mRNA Translation: AAH34867.1 AF109906 Genomic DNA Translation: AAC84161.1 U83841 Genomic DNA Translation: AAB41535.1 U83842 Genomic DNA Translation: AAB41536.1 |
CCDSi | CCDS28662.1 [P19426-2] |
PIRi | A40112 |
RefSeqi | NP_001039328.1, NM_001045863.1 [P19426-2] NP_001039329.1, NM_001045864.1 [P19426-2] NP_613046.2, NM_138580.2 [P19426-2] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M21332 Genomic DNA Translation: AAA39680.1 AK012389 mRNA Translation: BAB28206.1 BC034867 mRNA Translation: AAH34867.1 AF109906 Genomic DNA Translation: AAC84161.1 U83841 Genomic DNA Translation: AAB41535.1 U83842 Genomic DNA Translation: AAB41536.1 |
CCDSi | CCDS28662.1 [P19426-2] |
PIRi | A40112 |
RefSeqi | NP_001039328.1, NM_001045863.1 [P19426-2] NP_001039329.1, NM_001045864.1 [P19426-2] NP_613046.2, NM_138580.2 [P19426-2] |
3D structure databases
BMRBi | P19426 |
SMRi | P19426 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205393, 6 interactors |
IntActi | P19426, 1 interactor |
STRINGi | 10090.ENSMUSP00000134272 |
PTM databases
iPTMneti | P19426 |
PhosphoSitePlusi | P19426 |
Proteomic databases
jPOSTi | P19426 |
MaxQBi | P19426 |
PaxDbi | P19426 |
PeptideAtlasi | P19426 |
PRIDEi | P19426 |
Protocols and materials databases
Antibodypediai | 1924, 146 antibodies |
Genome annotation databases
Organism-specific databases
CTDi | 7936 |
MGIi | MGI:102744, Nelfe |
Phylogenomic databases
eggNOGi | ENOG502QQQ4, Eukaryota |
GeneTreei | ENSGT00630000089917 |
HOGENOMi | CLU_055643_0_0_1 |
InParanoidi | P19426 |
OMAi | RRQPIIE |
PhylomeDBi | P19426 |
TreeFami | TF324087 |
Enzyme and pathway databases
Reactomei | R-MMU-112382, Formation of RNA Pol II elongation complex R-MMU-113418, Formation of the Early Elongation Complex R-MMU-674695, RNA Polymerase II Pre-transcription Events R-MMU-6796648, TP53 Regulates Transcription of DNA Repair Genes R-MMU-75955, RNA Polymerase II Transcription Elongation |
Miscellaneous databases
BioGRID-ORCSi | 27632, 6 hits in 17 CRISPR screens |
ChiTaRSi | Nelfe, mouse |
PROi | PR:P19426 |
RNActi | P19426, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024369, Expressed in testis and 302 other tissues |
ExpressionAtlasi | P19426, baseline and differential |
Genevisiblei | P19426, MM |
Family and domain databases
CDDi | cd12305, RRM_NELFE, 1 hit |
Gene3Di | 3.30.70.330, 1 hit |
InterProi | View protein in InterPro IPR033102, NELFE IPR034637, NELFE_RRM IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
PANTHERi | PTHR17250, PTHR17250, 2 hits |
Pfami | View protein in Pfam PF00076, RRM_1, 1 hit |
SMARTi | View protein in SMART SM00360, RRM, 1 hit |
SUPFAMi | SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50102, RRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NELFE_MOUSE | |
Accessioni | P19426Primary (citable) accession number: P19426 Secondary accession number(s): P97485, P97486, Q9CZN3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 1, 1991 |
Last sequence update: | November 14, 2003 | |
Last modified: | February 10, 2021 | |
This is version 192 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families