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UniProtKB - P19406 (PPB4_BACSU)
Protein
Alkaline phosphatase 4
Gene
phoA
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalytic activityi
- EC:3.1.3.1PROSITE-ProRule annotation
Cofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 58 | MagnesiumBy similarity | 1 | |
Metal bindingi | 58 | Zinc 2By similarity | 1 | |
Active sitei | 108 | Phosphoserine intermediatePROSITE-ProRule annotation | 1 | |
Metal bindingi | 161 | MagnesiumBy similarity | 1 | |
Metal bindingi | 282 | MagnesiumBy similarity | 1 | |
Metal bindingi | 287 | Zinc 1By similarity | 1 | |
Metal bindingi | 291 | Zinc 1By similarity | 1 | |
Metal bindingi | 329 | Zinc 2By similarity | 1 | |
Metal bindingi | 330 | Zinc 2By similarity | 1 | |
Metal bindingi | 423 | Zinc 1By similarity | 1 |
GO - Molecular functioni
- alkaline phosphatase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- dephosphorylation Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | BSUB:BSU09410-MONOMER |
BRENDAi | 3.1.3.1, 658 |
Names & Taxonomyi
Protein namesi | Recommended name: Alkaline phosphatase 4 (EC:3.1.3.1)Alternative name(s): Alkaline phosphatase IV Short name: APase IV |
Gene namesi | Name:phoA Synonyms:phoAIV Ordered Locus Names:BSU09410 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 41 | 1 PublicationAdd BLAST | 41 | |
ChainiPRO_0000024011 | 42 – 461 | Alkaline phosphatase 4Add BLAST | 420 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P19406 |
Family & Domainsi
Sequence similaritiesi
Belongs to the alkaline phosphatase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1785, Bacteria |
InParanoidi | P19406 |
OMAi | NLTGMMM |
PhylomeDBi | P19406 |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P19406-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKMSLFQNM KSKLLPIAAV SVLTAGIFAG AELQQTEKAS AKKQDKAEIR
60 70 80 90 100
NVIVMIGDGM GTPYIRAYRS MKNNGDTPNN PKLTEFDRNL TGMMMTHPDD
110 120 130 140 150
PDYNITDSAA AGTALATGVK TYNNAIGVDK NGKKVKSVLE EAKQQGKSTG
160 170 180 190 200
LVATSEINHA TPAAYGAHNE SRKNMDQIAN SYMDDKIKGK HKIDVLLGGG
210 220 230 240 250
KSYFNRKDRN LTKEFKQAGY SYVTTKQALK KNKDQQVLGL FADGGLAKAL
260 270 280 290 300
DRDSKTPSLK DMTVSAIDRL NQNKKGFFLM VEGSQIDWAA HDNDTVGAMS
310 320 330 340 350
EVKDFEQAYK AAIEFAKKDK HTLVIATADH TTGGFTIGAN GEKNWHAEPI
360 370 380 390 400
LSAKKTPEFM AKKISEGKPV KDVLARYANL KVTSEEIKSV EAAAQADKSK
410 420 430 440 450
GASKAIIKIF NTRSNSGWTS TDHTGEEVPV YAYGPGKEKF RGLINNTDQA
460
NIIFKILKTG K
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 50 | R → K AA sequence (PubMed:2105301).Curated | 1 | |
Sequence conflicti | 208 | D → N in AAA18323 (PubMed:8113174).Curated | 1 | |
Sequence conflicti | 208 | D → N in CAA74486 (PubMed:9579061).Curated | 1 | |
Sequence conflicti | 208 | D → N (PubMed:1898729).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02550 Unassigned DNA Translation: AAA18323.1 Y14082 Genomic DNA Translation: CAA74486.1 AL009126 Genomic DNA Translation: CAB12780.2 L26337 Genomic DNA Translation: AAA22812.1 |
PIRi | B69676 |
RefSeqi | NP_388822.2, NC_000964.3 WP_010886458.1, NZ_JNCM01000035.1 |
Genome annotation databases
EnsemblBacteriai | CAB12780; CAB12780; BSU_09410 |
GeneIDi | 936265 |
KEGGi | bsu:BSU09410 |
PATRICi | fig|224308.43.peg.983 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U02550 Unassigned DNA Translation: AAA18323.1 Y14082 Genomic DNA Translation: CAA74486.1 AL009126 Genomic DNA Translation: CAB12780.2 L26337 Genomic DNA Translation: AAA22812.1 |
PIRi | B69676 |
RefSeqi | NP_388822.2, NC_000964.3 WP_010886458.1, NZ_JNCM01000035.1 |
3D structure databases
AlphaFoldDBi | P19406 |
SMRi | P19406 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU09410 |
Proteomic databases
PaxDbi | P19406 |
Genome annotation databases
EnsemblBacteriai | CAB12780; CAB12780; BSU_09410 |
GeneIDi | 936265 |
KEGGi | bsu:BSU09410 |
PATRICi | fig|224308.43.peg.983 |
Phylogenomic databases
eggNOGi | COG1785, Bacteria |
InParanoidi | P19406 |
OMAi | NLTGMMM |
PhylomeDBi | P19406 |
Enzyme and pathway databases
BioCyci | BSUB:BSU09410-MONOMER |
BRENDAi | 3.1.3.1, 658 |
Family and domain databases
CDDi | cd16012, ALP, 1 hit |
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR001952, Alkaline_phosphatase IPR018299, Alkaline_phosphatase_AS IPR017850, Alkaline_phosphatase_core_sf |
PANTHERi | PTHR11596, PTHR11596, 1 hit |
Pfami | View protein in Pfam PF00245, Alk_phosphatase, 1 hit |
PRINTSi | PR00113, ALKPHPHTASE |
SMARTi | View protein in SMART SM00098, alkPPc, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
PROSITEi | View protein in PROSITE PS00123, ALKALINE_PHOSPHATASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PPB4_BACSU | |
Accessioni | P19406Primary (citable) accession number: P19406 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | June 16, 2009 | |
Last modified: | May 25, 2022 | |
This is version 154 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families