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UniProtKB - P19388 (RPAB1_HUMAN)

Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC1

Gene

POLR2E

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).By similarity1 Publication

GO - Molecular functioni

  • DNA binding Source: InterPro
  • DNA-directed 5'-3' RNA polymerase activity Source: ProtInc
View the complete GO annotation on QuickGO ...

GO - Biological processi

View the complete GO annotation on QuickGO ...

Keywordsi

Biological processHost-virus interaction, Transcription

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5250924 B-WICH complex positively regulates rRNA expression
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73863 RNA Polymerase I Transcription Termination
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Short name:
RNA polymerases I, II, and III subunit ABC1
Alternative name(s):
DNA-directed RNA polymerase II 23 kDa polypeptide
DNA-directed RNA polymerase II subunit E
RPB5 homolog
XAP4
Gene namesi
Name:POLR2E
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

EuPathDBiHostDB:ENSG00000099817.11
HGNCiHGNC:9192 POLR2E
MIMi180664 gene
neXtProtiNX_P19388

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5434
OpenTargetsiENSG00000099817
PharmGKBiPA33512

Polymorphism and mutation databases

BioMutaiPOLR2E
DMDMi116242767

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001460751 – 210DNA-directed RNA polymerases I, II, and III subunit RPABC1Add BLAST210

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Cross-linki81Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiP19388
MaxQBiP19388
PaxDbiP19388
PeptideAtlasiP19388
PRIDEiP19388
ProteomicsDBi53653
TopDownProteomicsiP19388

2D gel databases

REPRODUCTION-2DPAGEiIPI00291093
SWISS-2DPAGEiP19388

PTM databases

iPTMnetiP19388
PhosphoSitePlusiP19388

Expressioni

Gene expression databases

BgeeiENSG00000099817 Expressed in 238 organ(s), highest expression level in heart left ventricle
CleanExiHS_POLR2E
ExpressionAtlasiP19388 baseline and differential
GenevisibleiP19388 HS

Organism-specific databases

HPAiHPA063030
HPA068992

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes consisting of at least 13, 12 and 17 subunits, respectively (By similarity). In RNA Pol II, this subunit is present in 2-fold molar excess over the other subunits. Interacts with URI1.By similarity3 Publications
(Microbial infection) Interacts with HBV protein X.1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi111430, 134 interactors
CORUMiP19388
DIPiDIP-56N
IntActiP19388, 67 interactors
MINTiP19388
STRINGi9606.ENSP00000215587

Structurei

3D structure databases

ProteinModelPortaliP19388
SMRiP19388
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.Curated

Phylogenomic databases

eggNOGiKOG3218 Eukaryota
COG2012 LUCA
GeneTreeiENSGT00390000013841
HOGENOMiHOG000205213
HOVERGENiHBG057019
InParanoidiP19388
KOiK03013
OMAiNQLMRIQ
OrthoDBiEOG091G0JI0
PhylomeDBiP19388

Family and domain databases

Gene3Di3.40.1340.10, 1 hit
3.90.940.20, 1 hit
HAMAPiMF_00025 RNApol_RpoH_RPB5, 1 hit
InterProiView protein in InterPro
IPR014381 DNA_RNA_pol_RPB5_euk/virus
IPR005571 RNA_pol_Rpb5_N
IPR036710 RNA_pol_Rpb5_N_sf
IPR000783 RNA_pol_subH/Rpb5_C
IPR020608 RNA_pol_subH/Rpb5_CS
IPR039531 Rpb5-like
IPR035913 RPB5-like_sf
IPR020609 RpoH/RPB5
PANTHERiPTHR10535 PTHR10535, 1 hit
PfamiView protein in Pfam
PF01191 RNA_pol_Rpb5_C, 1 hit
PF03871 RNA_pol_Rpb5_N, 1 hit
PIRSFiPIRSF000747 RPB5, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005155 RNA_pol_subH/Rpb5_C, 1 hit
SUPFAMiSSF53036 SSF53036, 1 hit
SSF55287 SSF55287, 1 hit
PROSITEiView protein in PROSITE
PS01110 RNA_POL_H_23KD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

P19388-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE 
        60         70         80         90        100
GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT 
       110        120        130        140        150
RALIVVQQGM TPSAKQSLVD MAPKYILEQF LQQELLINIT EHELVPEHVV 
       160        170        180        190        200
MTKEEVTELL ARYKLRENQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA 
       210
GRYITYRLVQ
Length:210
Mass (Da):24,551
Last modified:October 17, 2006 - v4
Checksum:i1E88AFDBCF9C8535
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WVZ9A0A087WVZ9_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2E
184Annotation score:
A0A087WWX0A0A087WWX0_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2E
134Annotation score:
A0A0A0MQR7A0A0A0MQR7_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2E
204Annotation score:
A0A087WVN5A0A087WVN5_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2E
83Annotation score:
K7EJ87K7EJ87_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2E
68Annotation score:
K7EQN2K7EQN2_HUMAN
DNA-directed RNA polymerases I, II,...
POLR2E
51Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti132Q → E (PubMed:2753903).Curated1
Sequence conflicti132Q → E (PubMed:7828586).Curated1
Sequence conflicti157T → S in BAA07406 (PubMed:7828586).Curated1
Sequence conflicti185I → V in AAH34144 (PubMed:15489334).Curated1
Sequence conflicti186K → R in BAA07406 (PubMed:7828586).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02825944S → F5 PublicationsCorresponds to variant dbSNP:rs12459404Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04965 mRNA Translation: AAA62401.1 Sequence problems.
S42643 mRNA Translation: AAB19339.1
D38251 mRNA Translation: BAA07406.1
AK312625 mRNA Translation: BAG35511.1
AC004151 Genomic DNA Translation: AAC03238.1
CH471139 Genomic DNA Translation: EAW69548.1
CH471139 Genomic DNA Translation: EAW69549.1
BC004441 mRNA Translation: AAH04441.1
BC034144 mRNA Translation: AAH34144.1
CCDSiCCDS12056.1
PIRiS52002 A32618
RefSeqiNP_002686.2, NM_002695.3
UniGeneiHs.24301

Genome annotation databases

EnsembliENST00000586746; ENSP00000464739; ENSG00000099817
ENST00000612655; ENSP00000485021; ENSG00000099817
ENST00000615234; ENSP00000478303; ENSG00000099817
GeneIDi5434
KEGGihsa:5434
UCSCiuc002lre.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04965 mRNA Translation: AAA62401.1 Sequence problems.
S42643 mRNA Translation: AAB19339.1
D38251 mRNA Translation: BAA07406.1
AK312625 mRNA Translation: BAG35511.1
AC004151 Genomic DNA Translation: AAC03238.1
CH471139 Genomic DNA Translation: EAW69548.1
CH471139 Genomic DNA Translation: EAW69549.1
BC004441 mRNA Translation: AAH04441.1
BC034144 mRNA Translation: AAH34144.1
CCDSiCCDS12056.1
PIRiS52002 A32618
RefSeqiNP_002686.2, NM_002695.3
UniGeneiHs.24301

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY6electron microscopy7.20E1-210[»]
5IY7electron microscopy8.60E1-210[»]
5IY8electron microscopy7.90E1-210[»]
5IY9electron microscopy6.30E1-210[»]
5IYAelectron microscopy5.40E1-210[»]
5IYBelectron microscopy3.90E1-210[»]
5IYCelectron microscopy3.90E1-210[»]
5IYDelectron microscopy3.90E1-210[»]
ProteinModelPortaliP19388
SMRiP19388
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111430, 134 interactors
CORUMiP19388
DIPiDIP-56N
IntActiP19388, 67 interactors
MINTiP19388
STRINGi9606.ENSP00000215587

PTM databases

iPTMnetiP19388
PhosphoSitePlusiP19388

Polymorphism and mutation databases

BioMutaiPOLR2E
DMDMi116242767

2D gel databases

REPRODUCTION-2DPAGEiIPI00291093
SWISS-2DPAGEiP19388

Proteomic databases

EPDiP19388
MaxQBiP19388
PaxDbiP19388
PeptideAtlasiP19388
PRIDEiP19388
ProteomicsDBi53653
TopDownProteomicsiP19388

Protocols and materials databases

DNASUi5434
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000586746; ENSP00000464739; ENSG00000099817
ENST00000612655; ENSP00000485021; ENSG00000099817
ENST00000615234; ENSP00000478303; ENSG00000099817
GeneIDi5434
KEGGihsa:5434
UCSCiuc002lre.5 human

Organism-specific databases

CTDi5434
DisGeNETi5434
EuPathDBiHostDB:ENSG00000099817.11
GeneCardsiPOLR2E
HGNCiHGNC:9192 POLR2E
HPAiHPA063030
HPA068992
MIMi180664 gene
neXtProtiNX_P19388
OpenTargetsiENSG00000099817
PharmGKBiPA33512
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3218 Eukaryota
COG2012 LUCA
GeneTreeiENSGT00390000013841
HOGENOMiHOG000205213
HOVERGENiHBG057019
InParanoidiP19388
KOiK03013
OMAiNQLMRIQ
OrthoDBiEOG091G0JI0
PhylomeDBiP19388

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-427413 NoRC negatively regulates rRNA expression
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5250924 B-WICH complex positively regulates rRNA expression
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73762 RNA Polymerase I Transcription Initiation
R-HSA-73772 RNA Polymerase I Promoter Escape
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73777 RNA Polymerase I Chain Elongation
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73863 RNA Polymerase I Transcription Termination
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Miscellaneous databases

ChiTaRSiPOLR2E human
GeneWikiiPOLR2E
GenomeRNAii5434
PROiPR:P19388
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000099817 Expressed in 238 organ(s), highest expression level in heart left ventricle
CleanExiHS_POLR2E
ExpressionAtlasiP19388 baseline and differential
GenevisibleiP19388 HS

Family and domain databases

Gene3Di3.40.1340.10, 1 hit
3.90.940.20, 1 hit
HAMAPiMF_00025 RNApol_RpoH_RPB5, 1 hit
InterProiView protein in InterPro
IPR014381 DNA_RNA_pol_RPB5_euk/virus
IPR005571 RNA_pol_Rpb5_N
IPR036710 RNA_pol_Rpb5_N_sf
IPR000783 RNA_pol_subH/Rpb5_C
IPR020608 RNA_pol_subH/Rpb5_CS
IPR039531 Rpb5-like
IPR035913 RPB5-like_sf
IPR020609 RpoH/RPB5
PANTHERiPTHR10535 PTHR10535, 1 hit
PfamiView protein in Pfam
PF01191 RNA_pol_Rpb5_C, 1 hit
PF03871 RNA_pol_Rpb5_N, 1 hit
PIRSFiPIRSF000747 RPB5, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD005155 RNA_pol_subH/Rpb5_C, 1 hit
SUPFAMiSSF53036 SSF53036, 1 hit
SSF55287 SSF55287, 1 hit
PROSITEiView protein in PROSITE
PS01110 RNA_POL_H_23KD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPAB1_HUMAN
AccessioniPrimary (citable) accession number: P19388
Secondary accession number(s): B2R6L4
, D6W5Y1, O43380, Q6PIH5, Q9BT06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: October 17, 2006
Last modified: November 7, 2018
This is version 196 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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