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Protein

DNA-directed RNA polymerase II subunit RPB3

Gene

POLR2C

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase II subunit RPB3Curated
Short name:
RNA polymerase II subunit 3
Short name:
RNA polymerase II subunit B3
Alternative name(s):
DNA-directed RNA polymerase II 33 kDa polypeptide
Short name:
RPB33
DNA-directed RNA polymerase II subunit C
RPB31By similarity
Gene namesi
Name:POLR2CImported
ORF Names:A-152E5.7
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000102978.12
HGNCiHGNC:9189 POLR2C
MIMi180663 gene
neXtProtiNX_P19387

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5432
OpenTargetsiENSG00000102978
PharmGKBiPA33509

Polymorphism and mutation databases

BioMutaiPOLR2C
DMDMi3915850

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001327432 – 275DNA-directed RNA polymerase II subunit RPB3Add BLAST274

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei124PhosphoserineCombined sources1
Modified residuei257PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP19387
PaxDbiP19387
PeptideAtlasiP19387
PRIDEiP19387
ProteomicsDBi53652

PTM databases

iPTMnetiP19387
PhosphoSitePlusiP19387

Expressioni

Gene expression databases

BgeeiENSG00000102978 Expressed in 233 organ(s), highest expression level in pituitary gland
CleanExiHS_POLR2C
ExpressionAtlasiP19387 baseline and differential
GenevisibleiP19387 HS

Organism-specific databases

HPAiHPA040919
HPA041826

Interactioni

Subunit structurei

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ATF4P188486EBI-394729,EBI-492498

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111428, 117 interactors
CORUMiP19387
DIPiDIP-32911N
IntActiP19387, 70 interactors
MINTiP19387
STRINGi9606.ENSP00000219252

Structurei

3D structure databases

ProteinModelPortaliP19387
SMRiP19387
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi88 – 97Cys-rich10

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1522 Eukaryota
COG0202 LUCA
GeneTreeiENSGT00640000091532
HOVERGENiHBG000897
InParanoidiP19387
KOiK03011
OMAiPENIVMM
OrthoDBiEOG091G0EGB
PhylomeDBiP19387
TreeFamiTF103038

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 1 hit
InterProiView protein in InterPro
IPR001514 DNA-dir_RNA_pol_30-40kDasu_CS
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR036603 RBP11-like
IPR036643 RNApol_insert_sf
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF01193 RNA_pol_L, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 1 hit
SSF56553 SSF56553, 1 hit
PROSITEiView protein in PROSITE
PS00446 RNA_POL_D_30KD, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

P19387-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV
60 70 80 90 100
QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCTC EEFCPECSVE
110 120 130 140 150
FTLDVRCNED QTRHVTSRDL ISNSPRVIPV TSRNRDNDPN DYVEQDDILI
160 170 180 190 200
VKLRKGQELR LRAYAKKGFG KEHAKWNPTA GVAFEYDPDN ALRHTVYPKP
210 220 230 240 250
EEWPKSEYSE LDEDESQAPY DPNGKPERFY YNVESCGSLR PETIVLSALS
260 270
GLKKKLSDLQ TQLSHEIQSD VLTIN
Length:275
Mass (Da):31,441
Last modified:December 15, 1998 - v2
Checksum:iEF663BE096046A4B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BRR2H3BRR2_HUMAN
DNA-directed RNA polymerase II subu...
POLR2C
46Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194H → T (PubMed:2187864).Curated1
Sequence conflicti194H → T (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05448 mRNA Translation: AAA36586.1
AF008443 mRNA Translation: AAC14355.1
AJ224143 mRNA Translation: CAA11842.1
AJ224144 mRNA Translation: CAA11843.1
AC004382 Genomic DNA Translation: AAC24309.1
BC000409 mRNA Translation: AAH00409.1
BC003159 mRNA Translation: AAH03159.1
BC028157 mRNA Translation: AAH28157.1
CCDSiCCDS10782.1
PIRiA36264
RefSeqiNP_116558.1, NM_032940.2
UniGeneiHs.79402

Genome annotation databases

EnsembliENST00000219252; ENSP00000219252; ENSG00000102978
GeneIDi5432
KEGGihsa:5432

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05448 mRNA Translation: AAA36586.1
AF008443 mRNA Translation: AAC14355.1
AJ224143 mRNA Translation: CAA11842.1
AJ224144 mRNA Translation: CAA11843.1
AC004382 Genomic DNA Translation: AAC24309.1
BC000409 mRNA Translation: AAH00409.1
BC003159 mRNA Translation: AAH03159.1
BC028157 mRNA Translation: AAH28157.1
CCDSiCCDS10782.1
PIRiA36264
RefSeqiNP_116558.1, NM_032940.2
UniGeneiHs.79402

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5IY6electron microscopy7.20C1-275[»]
5IY7electron microscopy8.60C1-275[»]
5IY8electron microscopy7.90C1-275[»]
5IY9electron microscopy6.30C1-275[»]
5IYAelectron microscopy5.40C1-275[»]
5IYBelectron microscopy3.90C1-275[»]
5IYCelectron microscopy3.90C1-275[»]
5IYDelectron microscopy3.90C1-275[»]
ProteinModelPortaliP19387
SMRiP19387
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111428, 117 interactors
CORUMiP19387
DIPiDIP-32911N
IntActiP19387, 70 interactors
MINTiP19387
STRINGi9606.ENSP00000219252

PTM databases

iPTMnetiP19387
PhosphoSitePlusiP19387

Polymorphism and mutation databases

BioMutaiPOLR2C
DMDMi3915850

Proteomic databases

EPDiP19387
PaxDbiP19387
PeptideAtlasiP19387
PRIDEiP19387
ProteomicsDBi53652

Protocols and materials databases

DNASUi5432
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219252; ENSP00000219252; ENSG00000102978
GeneIDi5432
KEGGihsa:5432

Organism-specific databases

CTDi5432
DisGeNETi5432
EuPathDBiHostDB:ENSG00000102978.12
GeneCardsiPOLR2C
HGNCiHGNC:9189 POLR2C
HPAiHPA040919
HPA041826
MIMi180663 gene
neXtProtiNX_P19387
OpenTargetsiENSG00000102978
PharmGKBiPA33509
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1522 Eukaryota
COG0202 LUCA
GeneTreeiENSGT00640000091532
HOVERGENiHBG000897
InParanoidiP19387
KOiK03011
OMAiPENIVMM
OrthoDBiEOG091G0EGB
PhylomeDBiP19387
TreeFamiTF103038

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-113418 Formation of the Early Elongation Complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167158 Formation of the HIV-1 Early Elongation Complex
R-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167242 Abortive elongation of HIV-1 transcript in the absence of Tat
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-168325 Viral Messenger RNA Synthesis
R-HSA-203927 MicroRNA (miRNA) biogenesis
R-HSA-452723 Transcriptional regulation of pluripotent stem cells
R-HSA-5578749 Transcriptional regulation by small RNAs
R-HSA-5601884 PIWI-interacting RNA (piRNA) biogenesis
R-HSA-5617472 Activation of anterior HOX genes in hindbrain development during early embryogenesis
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6803529 FGFR2 alternative splicing
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-72086 mRNA Capping
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72165 mRNA Splicing - Minor Pathway
R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE
R-HSA-8851708 Signaling by FGFR2 IIIa TM
R-HSA-9018519 Estrogen-dependent gene expression

Miscellaneous databases

GeneWikiiPOLR2C
GenomeRNAii5432
PROiPR:P19387
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000102978 Expressed in 233 organ(s), highest expression level in pituitary gland
CleanExiHS_POLR2C
ExpressionAtlasiP19387 baseline and differential
GenevisibleiP19387 HS

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 1 hit
InterProiView protein in InterPro
IPR001514 DNA-dir_RNA_pol_30-40kDasu_CS
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR036603 RBP11-like
IPR036643 RNApol_insert_sf
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF01193 RNA_pol_L, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 1 hit
SSF56553 SSF56553, 1 hit
PROSITEiView protein in PROSITE
PS00446 RNA_POL_D_30KD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPB3_HUMAN
AccessioniPrimary (citable) accession number: P19387
Secondary accession number(s): O15161
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: December 15, 1998
Last modified: November 7, 2018
This is version 196 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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