UniProtKB - P19387 (RPB3_HUMAN)
Protein
DNA-directed RNA polymerase II subunit RPB3
Gene
POLR2C
Organism
Homo sapiens (Human)
Status
Functioni
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).By similarity1 Publication
GO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
- protein dimerization activity Source: InterPro
GO - Biological processi
- 7-methylguanosine mRNA capping Source: Reactome
- fibroblast growth factor receptor signaling pathway Source: Reactome
- mRNA splicing, via spliceosome Source: Reactome
- positive regulation of viral transcription Source: Reactome
- regulation of gene silencing by miRNA Source: Reactome
- RNA metabolic process Source: Reactome
- snRNA transcription by RNA polymerase II Source: Reactome
- somatic stem cell population maintenance Source: Reactome
- transcription, DNA-templated Source: Reactome
- transcription by RNA polymerase II Source: UniProtKB
- transcription-coupled nucleotide-excision repair Source: Reactome
- transcription elongation from RNA polymerase II promoter Source: Reactome
- transcription initiation from RNA polymerase II promoter Source: Reactome
Keywordsi
Biological process | Transcription |
Enzyme and pathway databases
PathwayCommonsi | P19387 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-113418, Formation of the Early Elongation Complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167158, Formation of the HIV-1 Early Elongation Complex R-HSA-167160, RNA Pol II CTD phosphorylation and interaction with CE during HIV infection R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-203927, MicroRNA (miRNA) biogenesis R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5601884, PIWI-interacting RNA (piRNA) biogenesis R-HSA-5617472, Activation of anterior HOX genes in hindbrain development during early embryogenesis R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6781823, Formation of TC-NER Pre-Incision Complex R-HSA-6781827, Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-6782135, Dual incision in TC-NER R-HSA-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-6803529, FGFR2 alternative splicing R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-72086, mRNA Capping R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72165, mRNA Splicing - Minor Pathway R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-75955, RNA Polymerase II Transcription Elongation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-77075, RNA Pol II CTD phosphorylation and interaction with CE R-HSA-8851708, Signaling by FGFR2 IIIa TM R-HSA-9018519, Estrogen-dependent gene expression R-HSA-9670095, Inhibition of DNA recombination at telomere |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-directed RNA polymerase II subunit RPB3CuratedShort name: RNA polymerase II subunit 3 Short name: RNA polymerase II subunit B3 Alternative name(s): DNA-directed RNA polymerase II 33 kDa polypeptide Short name: RPB33 DNA-directed RNA polymerase II subunit C RPB31By similarity |
Gene namesi | Name:POLR2CImported ORF Names:A-152E5.7 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000102978.12 |
HGNCi | HGNC:9189, POLR2C |
MIMi | 180663, gene |
neXtProti | NX_P19387 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoskeleton
- microtubule cytoskeleton Source: HPA
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
- RNA polymerase II, core complex Source: UniProtKB
Keywords - Cellular componenti
DNA-directed RNA polymerase, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 5432 |
OpenTargetsi | ENSG00000102978 |
PharmGKBi | PA33509 |
Miscellaneous databases
Pharosi | P19387, Tbio |
Polymorphism and mutation databases
BioMutai | POLR2C |
DMDMi | 3915850 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000132743 | 2 – 275 | DNA-directed RNA polymerase II subunit RPB3Add BLAST | 274 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 124 | PhosphoserineCombined sources | 1 | |
Modified residuei | 257 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P19387 |
jPOSTi | P19387 |
MassIVEi | P19387 |
PaxDbi | P19387 |
PeptideAtlasi | P19387 |
PRIDEi | P19387 |
ProteomicsDBi | 53652 |
PTM databases
iPTMneti | P19387 |
PhosphoSitePlusi | P19387 |
Expressioni
Gene expression databases
Bgeei | ENSG00000102978, Expressed in islet of Langerhans and 246 other tissues |
ExpressionAtlasi | P19387, baseline and differential |
Genevisiblei | P19387, HS |
Organism-specific databases
HPAi | ENSG00000102978, Low tissue specificity |
Interactioni
Subunit structurei
Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.
1 PublicationBinary interactionsi
P19387
With | #Exp. | IntAct |
---|---|---|
ATF4 [P18848] | 6 | EBI-394729,EBI-492498 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 111428, 135 interactors |
CORUMi | P19387 |
DIPi | DIP-32911N |
IntActi | P19387, 79 interactors |
MINTi | P19387 |
STRINGi | 9606.ENSP00000219252 |
Miscellaneous databases
RNActi | P19387, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 88 – 97 | Cys-rich | 10 |
Sequence similaritiesi
Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.Curated
Phylogenomic databases
eggNOGi | KOG1522, Eukaryota |
GeneTreei | ENSGT00950000183100 |
HOGENOMi | CLU_038421_1_0_1 |
InParanoidi | P19387 |
OMAi | PENIVMM |
OrthoDBi | 834009at2759 |
PhylomeDBi | P19387 |
TreeFami | TF103038 |
Family and domain databases
Gene3Di | 2.170.120.12, 1 hit 3.30.1360.10, 1 hit |
InterProi | View protein in InterPro IPR001514, DNA-dir_RNA_pol_30-40kDasu_CS IPR011262, DNA-dir_RNA_pol_insert IPR011263, DNA-dir_RNA_pol_RpoA/D/Rpb3 IPR036603, RBP11-like IPR036643, RNApol_insert_sf |
Pfami | View protein in Pfam PF01000, RNA_pol_A_bac, 1 hit PF01193, RNA_pol_L, 1 hit |
SMARTi | View protein in SMART SM00662, RPOLD, 1 hit |
SUPFAMi | SSF55257, SSF55257, 1 hit SSF56553, SSF56553, 1 hit |
PROSITEi | View protein in PROSITE PS00446, RNA_POL_D_30KD, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P19387-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPYANQPTVR ITELTDENVK FIIENTDLAV ANSIRRVFIA EVPIIAIDWV
60 70 80 90 100
QIDANSSVLH DEFIAHRLGL IPLISDDIVD KLQYSRDCTC EEFCPECSVE
110 120 130 140 150
FTLDVRCNED QTRHVTSRDL ISNSPRVIPV TSRNRDNDPN DYVEQDDILI
160 170 180 190 200
VKLRKGQELR LRAYAKKGFG KEHAKWNPTA GVAFEYDPDN ALRHTVYPKP
210 220 230 240 250
EEWPKSEYSE LDEDESQAPY DPNGKPERFY YNVESCGSLR PETIVLSALS
260 270
GLKKKLSDLQ TQLSHEIQSD VLTIN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketH3BRR2 | H3BRR2_HUMAN | DNA-directed RNA polymerase II subu... | POLR2C | 46 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 194 | H → T (PubMed:2187864).Curated | 1 | |
Sequence conflicti | 194 | H → T (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05448 mRNA Translation: AAA36586.1 AF008443 mRNA Translation: AAC14355.1 AJ224143 mRNA Translation: CAA11842.1 AJ224144 mRNA Translation: CAA11843.1 AC004382 Genomic DNA Translation: AAC24309.1 BC000409 mRNA Translation: AAH00409.1 BC003159 mRNA Translation: AAH03159.1 BC028157 mRNA Translation: AAH28157.1 |
CCDSi | CCDS10782.1 |
PIRi | A36264 |
RefSeqi | NP_116558.1, NM_032940.2 |
Genome annotation databases
Ensembli | ENST00000219252; ENSP00000219252; ENSG00000102978 |
GeneIDi | 5432 |
KEGGi | hsa:5432 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J05448 mRNA Translation: AAA36586.1 AF008443 mRNA Translation: AAC14355.1 AJ224143 mRNA Translation: CAA11842.1 AJ224144 mRNA Translation: CAA11843.1 AC004382 Genomic DNA Translation: AAC24309.1 BC000409 mRNA Translation: AAH00409.1 BC003159 mRNA Translation: AAH03159.1 BC028157 mRNA Translation: AAH28157.1 |
CCDSi | CCDS10782.1 |
PIRi | A36264 |
RefSeqi | NP_116558.1, NM_032940.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5IY6 | electron microscopy | 7.20 | C | 1-275 | [»] | |
5IY7 | electron microscopy | 8.60 | C | 1-275 | [»] | |
5IY8 | electron microscopy | 7.90 | C | 1-275 | [»] | |
5IY9 | electron microscopy | 6.30 | C | 1-275 | [»] | |
5IYA | electron microscopy | 5.40 | C | 1-275 | [»] | |
5IYB | electron microscopy | 3.90 | C | 1-275 | [»] | |
5IYC | electron microscopy | 3.90 | C | 1-275 | [»] | |
5IYD | electron microscopy | 3.90 | C | 1-275 | [»] | |
6DRD | electron microscopy | 3.90 | C | 1-275 | [»] | |
6O9L | electron microscopy | 7.20 | C | 1-275 | [»] | |
SMRi | P19387 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111428, 135 interactors |
CORUMi | P19387 |
DIPi | DIP-32911N |
IntActi | P19387, 79 interactors |
MINTi | P19387 |
STRINGi | 9606.ENSP00000219252 |
PTM databases
iPTMneti | P19387 |
PhosphoSitePlusi | P19387 |
Polymorphism and mutation databases
BioMutai | POLR2C |
DMDMi | 3915850 |
Proteomic databases
EPDi | P19387 |
jPOSTi | P19387 |
MassIVEi | P19387 |
PaxDbi | P19387 |
PeptideAtlasi | P19387 |
PRIDEi | P19387 |
ProteomicsDBi | 53652 |
Protocols and materials databases
Antibodypediai | 28873, 166 antibodies |
DNASUi | 5432 |
Genome annotation databases
Ensembli | ENST00000219252; ENSP00000219252; ENSG00000102978 |
GeneIDi | 5432 |
KEGGi | hsa:5432 |
Organism-specific databases
CTDi | 5432 |
DisGeNETi | 5432 |
EuPathDBi | HostDB:ENSG00000102978.12 |
GeneCardsi | POLR2C |
HGNCi | HGNC:9189, POLR2C |
HPAi | ENSG00000102978, Low tissue specificity |
MIMi | 180663, gene |
neXtProti | NX_P19387 |
OpenTargetsi | ENSG00000102978 |
PharmGKBi | PA33509 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1522, Eukaryota |
GeneTreei | ENSGT00950000183100 |
HOGENOMi | CLU_038421_1_0_1 |
InParanoidi | P19387 |
OMAi | PENIVMM |
OrthoDBi | 834009at2759 |
PhylomeDBi | P19387 |
TreeFami | TF103038 |
Enzyme and pathway databases
PathwayCommonsi | P19387 |
Reactomei | R-HSA-112382, Formation of RNA Pol II elongation complex R-HSA-113418, Formation of the Early Elongation Complex R-HSA-167152, Formation of HIV elongation complex in the absence of HIV Tat R-HSA-167158, Formation of the HIV-1 Early Elongation Complex R-HSA-167160, RNA Pol II CTD phosphorylation and interaction with CE during HIV infection R-HSA-167161, HIV Transcription Initiation R-HSA-167162, RNA Polymerase II HIV Promoter Escape R-HSA-167172, Transcription of the HIV genome R-HSA-167200, Formation of HIV-1 elongation complex containing HIV-1 Tat R-HSA-167238, Pausing and recovery of Tat-mediated HIV elongation R-HSA-167242, Abortive elongation of HIV-1 transcript in the absence of Tat R-HSA-167243, Tat-mediated HIV elongation arrest and recovery R-HSA-167246, Tat-mediated elongation of the HIV-1 transcript R-HSA-167287, HIV elongation arrest and recovery R-HSA-167290, Pausing and recovery of HIV elongation R-HSA-168325, Viral Messenger RNA Synthesis R-HSA-203927, MicroRNA (miRNA) biogenesis R-HSA-5578749, Transcriptional regulation by small RNAs R-HSA-5601884, PIWI-interacting RNA (piRNA) biogenesis R-HSA-5617472, Activation of anterior HOX genes in hindbrain development during early embryogenesis R-HSA-674695, RNA Polymerase II Pre-transcription Events R-HSA-6781823, Formation of TC-NER Pre-Incision Complex R-HSA-6781827, Transcription-Coupled Nucleotide Excision Repair (TC-NER) R-HSA-6782135, Dual incision in TC-NER R-HSA-6782210, Gap-filling DNA repair synthesis and ligation in TC-NER R-HSA-6796648, TP53 Regulates Transcription of DNA Repair Genes R-HSA-6803529, FGFR2 alternative splicing R-HSA-6807505, RNA polymerase II transcribes snRNA genes R-HSA-72086, mRNA Capping R-HSA-72163, mRNA Splicing - Major Pathway R-HSA-72165, mRNA Splicing - Minor Pathway R-HSA-72203, Processing of Capped Intron-Containing Pre-mRNA R-HSA-73776, RNA Polymerase II Promoter Escape R-HSA-73779, RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953, RNA Polymerase II Transcription Initiation R-HSA-75955, RNA Polymerase II Transcription Elongation R-HSA-76042, RNA Polymerase II Transcription Initiation And Promoter Clearance R-HSA-77075, RNA Pol II CTD phosphorylation and interaction with CE R-HSA-8851708, Signaling by FGFR2 IIIa TM R-HSA-9018519, Estrogen-dependent gene expression R-HSA-9670095, Inhibition of DNA recombination at telomere |
Miscellaneous databases
BioGRID-ORCSi | 5432, 766 hits in 847 CRISPR screens |
ChiTaRSi | POLR2C, human |
GeneWikii | POLR2C |
GenomeRNAii | 5432 |
Pharosi | P19387, Tbio |
PROi | PR:P19387 |
RNActi | P19387, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000102978, Expressed in islet of Langerhans and 246 other tissues |
ExpressionAtlasi | P19387, baseline and differential |
Genevisiblei | P19387, HS |
Family and domain databases
Gene3Di | 2.170.120.12, 1 hit 3.30.1360.10, 1 hit |
InterProi | View protein in InterPro IPR001514, DNA-dir_RNA_pol_30-40kDasu_CS IPR011262, DNA-dir_RNA_pol_insert IPR011263, DNA-dir_RNA_pol_RpoA/D/Rpb3 IPR036603, RBP11-like IPR036643, RNApol_insert_sf |
Pfami | View protein in Pfam PF01000, RNA_pol_A_bac, 1 hit PF01193, RNA_pol_L, 1 hit |
SMARTi | View protein in SMART SM00662, RPOLD, 1 hit |
SUPFAMi | SSF55257, SSF55257, 1 hit SSF56553, SSF56553, 1 hit |
PROSITEi | View protein in PROSITE PS00446, RNA_POL_D_30KD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPB3_HUMAN | |
Accessioni | P19387Primary (citable) accession number: P19387 Secondary accession number(s): O15161 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | December 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 211 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Human chromosome 16
Human chromosome 16: entries, gene names and cross-references to MIM