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Protein

Thymidylate synthase

Gene

thyA

Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei153NucleophileUniRule annotation1
Binding sitei1815,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei189dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi132 – 133dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi178 – 181dUMPUniRule annotation4
Nucleotide bindingi219 – 221dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

BioCyciLLAC272623:L182559-MONOMER
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:LL1541
ORF Names:L182559
OrganismiLactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Taxonomic identifieri272623 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus
Proteomesi
  • UP000002196 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409691 – 279Thymidylate synthaseAdd BLAST279

Proteomic databases

PaxDbiP19368

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272623.L182559

Structurei

3D structure databases

ProteinModelPortaliP19368
SMRiP19368
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257898
KOiK00560
OMAiWNEWEVG

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

P19368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTYADKIFKQ NIQNILDNGV FSENARPKYK DGQTANSKYV TGSFVTYDLQ
60 70 80 90 100
KGEFPITTLR PIPIKSAIKE LMWIYQDQTS ELAILEEKYG VKYWGEWGIG
110 120 130 140 150
DGTIGQRYGA TVKKYNIIGK LLDGLAKNPW NRRNIINLWQ YEDFEETEGL
160 170 180 190 200
LPCAFQTMFD VRREQDGQIY LDATLIQRSN DMLVAHHINA MQYVALQMMI
210 220 230 240 250
AKHFSWKVGK FFYFVNNLHI YDNQFEQANE LVKRTASDKE PRLVLNVPDG
260 270
TNFFDIKPED FELVDYEPVK PQLKFDLAI
Length:279
Mass (Da):32,499
Last modified:April 27, 2001 - v2
Checksum:iCE0B97935D8DA1F8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6 – 7KI → QV in AAA25221 (PubMed:2117882).Curated2
Sequence conflicti34T → M in AAA25221 (PubMed:2117882).Curated1
Sequence conflicti83 – 84AI → SV in AAA25221 (PubMed:2117882).Curated2
Sequence conflicti123D → E in AAA25221 (PubMed:2117882).Curated1
Sequence conflicti165Q → K in AAA25221 (PubMed:2117882).Curated1
Sequence conflicti232V → M in AAA25221 (PubMed:2117882).Curated1
Sequence conflicti238D → E in AAA25221 (PubMed:2117882).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M33770 Genomic DNA Translation: AAA25221.1
AE005176 Genomic DNA Translation: AAK05639.1
PIRiA43797
E86817
RefSeqiNP_267697.1, NC_002662.1
WP_003130015.1, NC_002662.1

Genome annotation databases

EnsemblBacteriaiAAK05639; AAK05639; L182559
GeneIDi1115198
KEGGilla:L182559
PATRICifig|272623.7.peg.1652

Similar proteinsi

Entry informationi

Entry nameiTYSY_LACLA
AccessioniPrimary (citable) accession number: P19368
Secondary accession number(s): Q9CFD8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: April 27, 2001
Last modified: June 20, 2018
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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