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Entry version 166 (13 Nov 2019)
Sequence version 2 (01 Nov 1997)
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Protein

Respiratory nitrate reductase 2 beta chain

Gene

narY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons are transferred from the gamma chain to the molybdenum cofactor of the alpha subunit.

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the ‘Description’ field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi16Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi19Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi22Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi26Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi183Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi186Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi191Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi195Iron-sulfur 4 (3Fe-4S)By similarity1
Metal bindingi216Iron-sulfur 4 (3Fe-4S)By similarity1
Metal bindingi222Iron-sulfur 4 (3Fe-4S)By similarity1
Metal bindingi226Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi243Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi246Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi258Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi262Iron-sulfur 1 (4Fe-4S)By similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:NARY-MONOMER
ECOL316407:JW1462-MONOMER
MetaCyc:NARY-MONOMER

Protein family/group databases

Transport Classification Database

More...
TCDBi
5.A.3.1.2 the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Respiratory nitrate reductase 2 beta chain (EC:1.7.5.1)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:narY
Ordered Locus Names:b1467, JW1462
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000967341 – 514Respiratory nitrate reductase 2 beta chainAdd BLAST514

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P19318

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P19318

PRoteomics IDEntifications database

More...
PRIDEi
P19318

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Dimer of heterotrimers each composed of an alpha, a beta and a gamma chain. Alpha and beta are catalytic chains; gamma chains are involved in binding the enzyme complex to the cytoplasmic membrane.

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4262894, 13 interactors
850395, 5 interactors

Database of interacting proteins

More...
DIPi
DIP-10323N

Protein interaction database and analysis system

More...
IntActi
P19318, 14 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b1467

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P19318

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini7 – 354Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST29
Domaini174 – 2054Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST32
Domaini207 – 2364Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30

Keywords - Domaini

Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4108IUE Bacteria
COG1140 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000237353

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P19318

KEGG Orthology (KO)

More...
KOi
K00371

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P19318

Family and domain databases

Conserved Domains Database

More...
CDDi
cd10557 NarH_beta-like, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.3650.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR029263 Nitr_red_bet_C
IPR038262 Nitr_red_bet_C_sf
IPR006547 NO3_Rdtase_bsu

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13247 Fer4_11, 1 hit
PF14711 Nitr_red_bet_C, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01660 narH, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51379 4FE4S_FER_2, 3 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P19318-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKIRSQVGMV LNLDKCIGCH TCSVTCKNVW TGREGMEYAW FNNVETKPGI
60 70 80 90 100
GYPKNWEDQE EWQGGWVRDV NGKIRPRLGN KMGVITKIFA NPVVPQIDDY
110 120 130 140 150
YEPFTFDYEH LHSAPEGKHI PTARPRSLID GKRMDKVIWG PNWEELLGGE
160 170 180 190 200
FEKRARDRNF EAMQKEMYGQ FENTFMMYLP RLCEHCLNPS CVATCPSGAI
210 220 230 240 250
YKREEDGIVL IDQDKCRGWR LCISGCPYKK IYFNWKSGKS EKCIFCYPRI
260 270 280 290 300
ESGQPTVCSE TCVGRIRYLG VLLYDADRIE EAASTEREVD LYERQCEVFL
310 320 330 340 350
DPHDPSVIEE ALKQGIPQNV IDAAQRSPVY KMAMDWKLAL PLHPEYRTLP
360 370 380 390 400
MVWYVPPLSP IQSYADAGGL PKSEGVLPAI ESLRIPVQYL ANMLSAGDTG
410 420 430 440 450
PVLRALKRMM AMRHYMRSQT VEGVTDTRAI DEVGLSVAQV EEMYRYLAIA
460 470 480 490 500
NYEDRFVIPT SHREMAGDAF AERNGCGFTF GDGCHGSDSK FNLFNSSRID
510
AINITEVRDK AEGE
Length:514
Mass (Da):58,558
Last modified:November 1, 1997 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i82D997DB093CD818
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti325 – 326QR → HG in CAA34965 (PubMed:2233673).Curated2

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X17110 Genomic DNA Translation: CAA34965.1
U00096 Genomic DNA Translation: AAC74549.1
AP009048 Genomic DNA Translation: BAA15104.1

Protein sequence database of the Protein Information Resource

More...
PIRi
F64899

NCBI Reference Sequences

More...
RefSeqi
NP_415984.1, NC_000913.3
WP_000702535.1, NZ_SSZK01000038.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC74549; AAC74549; b1467
BAA15104; BAA15104; BAA15104

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
946034

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW1462
eco:b1467

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.801

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X17110 Genomic DNA Translation: CAA34965.1
U00096 Genomic DNA Translation: AAC74549.1
AP009048 Genomic DNA Translation: BAA15104.1
PIRiF64899
RefSeqiNP_415984.1, NC_000913.3
WP_000702535.1, NZ_SSZK01000038.1

3D structure databases

SMRiP19318
ModBaseiSearch...

Protein-protein interaction databases

BioGridi4262894, 13 interactors
850395, 5 interactors
DIPiDIP-10323N
IntActiP19318, 14 interactors
STRINGi511145.b1467

Protein family/group databases

TCDBi5.A.3.1.2 the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

Proteomic databases

jPOSTiP19318
PaxDbiP19318
PRIDEiP19318

Genome annotation databases

EnsemblBacteriaiAAC74549; AAC74549; b1467
BAA15104; BAA15104; BAA15104
GeneIDi946034
KEGGiecj:JW1462
eco:b1467
PATRICifig|1411691.4.peg.801

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0641

Phylogenomic databases

eggNOGiENOG4108IUE Bacteria
COG1140 LUCA
HOGENOMiHOG000237353
InParanoidiP19318
KOiK00371
PhylomeDBiP19318

Enzyme and pathway databases

BioCyciEcoCyc:NARY-MONOMER
ECOL316407:JW1462-MONOMER
MetaCyc:NARY-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P19318

Family and domain databases

CDDicd10557 NarH_beta-like, 1 hit
Gene3Di1.10.3650.10, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR029263 Nitr_red_bet_C
IPR038262 Nitr_red_bet_C_sf
IPR006547 NO3_Rdtase_bsu
PfamiView protein in Pfam
PF13247 Fer4_11, 1 hit
PF14711 Nitr_red_bet_C, 1 hit
TIGRFAMsiTIGR01660 narH, 1 hit
PROSITEiView protein in PROSITE
PS51379 4FE4S_FER_2, 3 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNARY_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P19318
Secondary accession number(s): P78267
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1997
Last modified: November 13, 2019
This is version 166 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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