UniProtKB - P19253 (RL13A_MOUSE)
Protein
60S ribosomal protein L13a
Gene
Rpl13a
Organism
Mus musculus (Mouse)
Status
Functioni
Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex.By similarity1 Publication
GO - Molecular functioni
- mRNA binding Source: MGI
- structural constituent of ribosome Source: GO_Central
GO - Biological processi
- cellular response to interferon-gamma Source: UniProtKB
- homeostatic process Source: MGI
- lung morphogenesis Source: MGI
- macrophage chemotaxis Source: MGI
- negative regulation of formation of translation preinitiation complex Source: MGI
- negative regulation of translation Source: UniProtKB
- response to lipopolysaccharide Source: MGI
- translation Source: InterPro
Keywordsi
Molecular function | Ribonucleoprotein, Ribosomal protein, Tumor antigen |
Biological process | Translation regulation |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72689, Formation of a pool of free 40S subunits R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: 60S ribosomal protein L13aAlternative name(s): Transplantation antigen P198 Tum-P198 antigen |
Gene namesi | Name:Rpl13a Synonyms:P198, Tstap198-7 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1351455, Rpl13a |
Subcellular locationi
Cytosol
- cytosolic large ribosomal subunit Source: MGI
Other locations
- GAIT complex Source: UniProtKB
- ribonucleoprotein complex Source: MGI
- ribosome Source: MGI
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 77 | S → A: Loss of interferon-gamma induced phosphorylation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000133770 | 2 – 203 | 60S ribosomal protein L13aAdd BLAST | 202 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 59 | Citrulline1 Publication | 1 | |
Modified residuei | 77 | Phosphoserine; by ZIPK/DAPK31 Publication | 1 | |
Modified residuei | 140 | Citrulline1 Publication | 1 | |
Modified residuei | 191 | N6-acetyllysineBy similarity | 1 |
Post-translational modificationi
Phosphorylation at Ser-77 upon interferon-gamma treatment in macrophages involves a DAPK1-DAPK3 kinase cascade and is causing release from the ribosome, association with the GAIT complex and subsequent involvement in transcript-selective translation inhibition.1 Publication
Citrullinated by PADI4.1 Publication
Keywords - PTMi
Acetylation, Citrullination, PhosphoproteinProteomic databases
EPDi | P19253 |
jPOSTi | P19253 |
PaxDbi | P19253 |
PeptideAtlasi | P19253 |
PRIDEi | P19253 |
PTM databases
iPTMneti | P19253 |
PhosphoSitePlusi | P19253 |
SwissPalmi | P19253 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000074129, Expressed in pancreas and 54 other tissues |
Genevisiblei | P19253, MM |
Interactioni
Subunit structurei
Component of the 60S ribosome.
Component of the GAIT complex.
Interacts with EIF4G1 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 204356, 101 interactors |
ComplexPortali | CPX-5262, 60S cytosolic large ribosomal subunit |
CORUMi | P19253 |
IntActi | P19253, 10 interactors |
MINTi | P19253 |
STRINGi | 10090.ENSMUSP00000115722 |
Miscellaneous databases
RNActi | P19253, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the universal ribosomal protein uL13 family.Curated
Phylogenomic databases
eggNOGi | KOG3204, Eukaryota |
GeneTreei | ENSGT00390000010799 |
HOGENOMi | CLU_076922_0_0_1 |
InParanoidi | P19253 |
OrthoDBi | 1312756at2759 |
TreeFami | TF300159 |
Family and domain databases
CDDi | cd00392, Ribosomal_L13, 1 hit |
Gene3Di | 3.90.1180.10, 1 hit |
HAMAPi | MF_01366, Ribosomal_L13, 1 hit |
InterProi | View protein in InterPro IPR005822, Ribosomal_L13 IPR023563, Ribosomal_L13_CS IPR005755, Ribosomal_L13_euk/arc IPR036899, Ribosomal_L13_sf |
PANTHERi | PTHR11545, PTHR11545, 1 hit |
Pfami | View protein in Pfam PF00572, Ribosomal_L13, 1 hit |
SUPFAMi | SSF52161, SSF52161, 1 hit |
TIGRFAMsi | TIGR01077, L13_A_E, 1 hit |
PROSITEi | View protein in PROSITE PS00783, RIBOSOMAL_L13, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 10 potential isoforms that are computationally mapped.Show allAlign All
P19253-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAEGQVLVLD GRGHLLGRLA AIVAKQVLLG RKVVVVRCEG INISGNFYRN
60 70 80 90 100
KLKYLAFLRK RMNTNPSRGP YHFRAPSRIF WRTVRGMLPH KTKRGQAALE
110 120 130 140 150
RLKVLDGIPP PYDKKKRMVV PAALKVVRLK PTRKFAYLGR LAHEVGWKYQ
160 170 180 190 200
AVTATLEEKR KEKAKMHYRK KKQILRLRKQ AEKNVEKKIC KFTEVLKTNG
LLV
Computationally mapped potential isoform sequencesi
There are 10 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1B0GTA1 | A0A1B0GTA1_MOUSE | 60S ribosomal protein L13a | Rpl13a | 177 | Annotation score: | ||
A0A1B0GSB2 | A0A1B0GSB2_MOUSE | 60S ribosomal protein L13a | Rpl13a | 148 | Annotation score: | ||
A0A1B0GSQ6 | A0A1B0GSQ6_MOUSE | 60S ribosomal protein L13a | Rpl13a | 167 | Annotation score: | ||
A0A1B0GT00 | A0A1B0GT00_MOUSE | 60S ribosomal protein L13a | Rpl13a | 133 | Annotation score: | ||
A0A1B0GSC2 | A0A1B0GSC2_MOUSE | 60S ribosomal protein L13a | Rpl13a | 122 | Annotation score: | ||
A0A1B0GRH1 | A0A1B0GRH1_MOUSE | 60S ribosomal protein L13a | Rpl13a | 128 | Annotation score: | ||
A0A1B0GSJ2 | A0A1B0GSJ2_MOUSE | 60S ribosomal protein L13a | Rpl13a | 110 | Annotation score: | ||
A0A1B0GSL5 | A0A1B0GSL5_MOUSE | 60S ribosomal protein L13a | Rpl13a | 110 | Annotation score: | ||
A0A1B0GQW6 | A0A1B0GQW6_MOUSE | 60S ribosomal protein L13a | Rpl13a | 69 | Annotation score: | ||
A0A1B0GSF0 | A0A1B0GSF0_MOUSE | 60S ribosomal protein L13a | Rpl13a | 131 | Annotation score: |
Polymorphismi
The antigenic allele of P198 differs from the normal allele by a single mutation. The TUM- mutation P198 generates a new epitope recognized by syngeneic T-cells.
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 154 | A → T in allele TUM-. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51528 Genomic DNA Translation: CAA35908.1 BC082289 mRNA Translation: AAH82289.1 |
CCDSi | CCDS21230.1 |
PIRi | JL0149 |
RefSeqi | NP_033464.2, NM_009438.5 |
Genome annotation databases
Ensembli | ENSMUST00000150350; ENSMUSP00000115722; ENSMUSG00000074129 |
GeneIDi | 22121 |
KEGGi | mmu:22121 |
UCSCi | uc009gtj.2, mouse |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X51528 Genomic DNA Translation: CAA35908.1 BC082289 mRNA Translation: AAH82289.1 |
CCDSi | CCDS21230.1 |
PIRi | JL0149 |
RefSeqi | NP_033464.2, NM_009438.5 |
3D structure databases
SMRi | P19253 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204356, 101 interactors |
ComplexPortali | CPX-5262, 60S cytosolic large ribosomal subunit |
CORUMi | P19253 |
IntActi | P19253, 10 interactors |
MINTi | P19253 |
STRINGi | 10090.ENSMUSP00000115722 |
PTM databases
iPTMneti | P19253 |
PhosphoSitePlusi | P19253 |
SwissPalmi | P19253 |
Proteomic databases
EPDi | P19253 |
jPOSTi | P19253 |
PaxDbi | P19253 |
PeptideAtlasi | P19253 |
PRIDEi | P19253 |
Genome annotation databases
Ensembli | ENSMUST00000150350; ENSMUSP00000115722; ENSMUSG00000074129 |
GeneIDi | 22121 |
KEGGi | mmu:22121 |
UCSCi | uc009gtj.2, mouse |
Organism-specific databases
CTDi | 23521 |
MGIi | MGI:1351455, Rpl13a |
Phylogenomic databases
eggNOGi | KOG3204, Eukaryota |
GeneTreei | ENSGT00390000010799 |
HOGENOMi | CLU_076922_0_0_1 |
InParanoidi | P19253 |
OrthoDBi | 1312756at2759 |
TreeFami | TF300159 |
Enzyme and pathway databases
Reactomei | R-MMU-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-MMU-1799339, SRP-dependent cotranslational protein targeting to membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-72689, Formation of a pool of free 40S subunits R-MMU-72706, GTP hydrolysis and joining of the 60S ribosomal subunit R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 22121, 8 hits in 14 CRISPR screens |
ChiTaRSi | Rpl13a, mouse |
PROi | PR:P19253 |
RNActi | P19253, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000074129, Expressed in pancreas and 54 other tissues |
Genevisiblei | P19253, MM |
Family and domain databases
CDDi | cd00392, Ribosomal_L13, 1 hit |
Gene3Di | 3.90.1180.10, 1 hit |
HAMAPi | MF_01366, Ribosomal_L13, 1 hit |
InterProi | View protein in InterPro IPR005822, Ribosomal_L13 IPR023563, Ribosomal_L13_CS IPR005755, Ribosomal_L13_euk/arc IPR036899, Ribosomal_L13_sf |
PANTHERi | PTHR11545, PTHR11545, 1 hit |
Pfami | View protein in Pfam PF00572, Ribosomal_L13, 1 hit |
SUPFAMi | SSF52161, SSF52161, 1 hit |
TIGRFAMsi | TIGR01077, L13_A_E, 1 hit |
PROSITEi | View protein in PROSITE PS00783, RIBOSOMAL_L13, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RL13A_MOUSE | |
Accessioni | P19253Primary (citable) accession number: P19253 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 182 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Ribosomal proteins
Ribosomal proteins families and list of entries