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Protein

Methylated-DNA--protein-cysteine methyltransferase, inducible

Gene

adaB

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.2 Publications

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation1 Publication
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei141Nucleophile; methyl group acceptor1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciBSUB:BSU01820-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferase, inducible (EC:2.1.1.631 Publication)
Alternative name(s):
O-6-methylguanine-DNA alkyltransferase
O6-methylguanine-DNA methyltransferase
Short name:
MGMT
Gene namesi
Name:adaB
Ordered Locus Names:BSU01820
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi141C → Y: Loss of activity. 1 Publication1
Mutagenesisi167L → P: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393741 – 179Methylated-DNA--protein-cysteine methyltransferase, inducibleAdd BLAST179

Proteomic databases

PaxDbiP19220
PRIDEiP19220

Expressioni

Inductioni

Up-regulated by methylated AdaA in response to the exposure to alkylating agents such as MNNG.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001028

Structurei

3D structure databases

ProteinModelPortaliP19220
SMRiP19220
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.Curated

Phylogenomic databases

eggNOGiENOG4105K85 Bacteria
COG0350 LUCA
HOGENOMiHOG000244137
InParanoidiP19220
KOiK13531
OMAiRFHIAAT
PhylomeDBiP19220

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR008332 MethylG_MeTrfase_N
IPR036631 MGMT_N_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
PF02870 Methyltransf_1N, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
SSF53155 SSF53155, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

P19220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METNKPTLYW SLLMFKDWNF YIASTLKGLV FVGSQNKPIE ELFEWARKRF
60 70 80 90 100
PGSLLVEDDD KLEPYAVEIT QYLEGKRKNF TVPVEYAGTQ FQLAVWNALC
110 120 130 140 150
EIPYGQTKSY SDIANDINKP AAVRAVGAAI GANPVLITVP CHRVIGKNGS
160 170
LTGYRGGFEM KTLLLDLEKR ASSEMDVPH
Length:179
Mass (Da):20,124
Last modified:November 1, 1990 - v1
Checksum:iBC30C65C6B76B64A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53399 Genomic DNA Translation: CAA37476.1
AB006424 Genomic DNA Translation: BAA33075.1
AL009126 Genomic DNA Translation: CAB11958.1
PIRiS11564 XUBSMB
RefSeqiNP_388063.1, NC_000964.3
WP_003246311.1, NZ_JNCM01000030.1

Genome annotation databases

EnsemblBacteriaiCAB11958; CAB11958; BSU01820
GeneIDi938673
KEGGibsu:BSU01820
PATRICifig|224308.179.peg.188

Similar proteinsi

Entry informationi

Entry nameiADAB_BACSU
AccessioniPrimary (citable) accession number: P19220
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: May 23, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

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