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Protein

Eukaryotic translation initiation factor 5A-2

Gene

ANB1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Essential for polarized growth, a process necessary for G1/S transition. May mediate large range of effects of the polyamine spermidine in the cell (By similarity).By similarity4 Publications

Miscellaneous

There are two genes for eIF-5A in yeast.
Present with 14200 molecules/cell in log phase SD medium.1 Publication

Caution

Was originally thought to be a translation initiation factor but further analysis (PubMed:19424157 and PubMed:19338753) clearly suggests that it is involved in translation elongation and not translation initiation.1 Publication

GO - Molecular functioni

GO - Biological processi

  • positive regulation of translational elongation Source: SGD
  • positive regulation of translational termination Source: SGD
  • translational frameshifting Source: SGD

Keywordsi

Molecular functionElongation factor, RNA-binding
Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyciYEAST:G3O-31682-MONOMER
ReactomeiR-SCE-204626 Hypusine synthesis from eIF5A-lysine

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5A-2
Short name:
eIF-5A-2
Alternative name(s):
Anaerobically induced protein 1
Hypusine-containing protein HP1
eIF-4D
Gene namesi
Name:ANB1
Synonyms:HYP1, TIF51B
Ordered Locus Names:YJR047C
ORF Names:J1651
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR047C
SGDiS000003808 ANB1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001424872 – 157Eukaryotic translation initiation factor 5A-2Add BLAST156

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineCombined sources1
Modified residuei7PhosphothreonineCombined sources1
Modified residuei51Hypusine1 Publication1
Modified residuei74PhosphoserineBy similarity1
Cross-linki86Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Keywords - PTMi

Acetylation, Hypusine, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP19211
PaxDbiP19211
PRIDEiP19211

PTM databases

iPTMnetiP19211

Expressioni

Inductioni

Expressed in anaerobic conditions. Down-regulated by heme and the ROX1 and REO1 transcription factors in aerobic conditions.5 Publications

Interactioni

Subunit structurei

Homodimer. Interacts with DYS1 and LIA1.By similarity

Protein-protein interaction databases

BioGridi33800, 54 interactors
IntActiP19211, 3 interactors
MINTiP19211
STRINGi4932.YJR047C

Structurei

3D structure databases

ProteinModelPortaliP19211
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eIF-5A family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000003738
HOGENOMiHOG000106270
InParanoidiP19211
KOiK03263
OMAiCVIAIKT
OrthoDBiEOG092C4XQW

Family and domain databases

Gene3Di2.30.30.30, 1 hit
InterProiView protein in InterPro
IPR001884 IF5A-like
IPR012340 NA-bd_OB-fold
IPR014722 Rib_L2_dom2
IPR019769 Trans_elong_IF5A_hypusine_site
IPR020189 Transl_elong_IF5A_C
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR11673 PTHR11673, 1 hit
PfamiView protein in Pfam
PF01287 eIF-5a, 1 hit
PIRSFiPIRSF003025 eIF5A, 1 hit
SMARTiView protein in SMART
SM01376 eIF-5a, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00037 eIF_5A, 1 hit
PROSITEiView protein in PROSITE
PS00302 IF5A_HYPUSINE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19211-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDEEHTFEN ADAGASATYP MQCSALRKNG FVVIKGRPCK IVDMSTSKTG
60 70 80 90 100
KHGHAKVHLV TLDIFTGKKL EDLSPSTHNL EVPFVKRSEY QLLDIDDGYL
110 120 130 140 150
SLMTMDGETK DDVKAPEGEL GDSMQAAFDE GKDLMVTIIS AMGEEAAISF

KEAPRSD
Length:157
Mass (Da):17,131
Last modified:January 23, 2007 - v3
Checksum:iA578164B760ED9E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05455 Genomic DNA Translation: AAA34425.1
M63542 Genomic DNA Translation: AAA35156.1
M60477 Genomic DNA Translation: AAA34424.1
L36344 Genomic DNA Translation: AAA88750.1
Z49547 Genomic DNA Translation: CAA89575.1
AY557894 Genomic DNA Translation: AAS56220.1
X56235 Genomic DNA Translation: CAA39692.1
BK006943 Genomic DNA Translation: DAA08834.1
PIRiB40259 FIBYA2
RefSeqiNP_012581.3, NM_001181705.3

Genome annotation databases

EnsemblFungiiYJR047C; YJR047C; YJR047C
GeneIDi853506
KEGGisce:YJR047C

Similar proteinsi

Entry informationi

Entry nameiIF5A2_YEAST
AccessioniPrimary (citable) accession number: P19211
Secondary accession number(s): D6VWL8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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