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Protein

Manganese peroxidase H4

Gene
N/A
Organism
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of Mn2+ to Mn3+. The latter, acting as a diffusible redox mediator, is capable of oxidizing a variety of lignin compounds.1 Publication

Catalytic activityi

2 Mn2+ + 2 H+ + H2O2 = 2 Mn3+ + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • heme bPROSITE-ProRule annotationNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
  • Ca2+PROSITE-ProRule annotationNote: Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi59ManganeseBy similarity1
Metal bindingi63ManganeseBy similarity1
Sitei66Transition state stabilizerPROSITE-ProRule annotation1
Active sitei70Proton acceptorPROSITE-ProRule annotation1
Metal bindingi71Calcium 1PROSITE-ProRule annotation1
Metal bindingi86Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi88Calcium 1PROSITE-ProRule annotation1
Metal bindingi90Calcium 1PROSITE-ProRule annotation1
Metal bindingi197Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi198Calcium 2PROSITE-ProRule annotation1
Metal bindingi203ManganeseBy similarity1
Metal bindingi215Calcium 2PROSITE-ProRule annotation1
Metal bindingi217Calcium 2PROSITE-ProRule annotation1
Metal bindingi220Calcium 2; via carbonyl oxygenPROSITE-ProRule annotation1
Metal bindingi222Calcium 2PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide, Lignin degradation
LigandCalcium, Heme, Iron, Manganese, Metal-binding

Protein family/group databases

CAZyiAA2 Auxiliary Activities 2
mycoCLAPiMPO2D_PHACH
PeroxiBasei2383 PcMnP02_RP78

Names & Taxonomyi

Protein namesi
Recommended name:
Manganese peroxidase H4 (EC:1.11.1.13)
Alternative name(s):
MP-I
Peroxidase manganese-dependent H4
OrganismiPhanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)
Taxonomic identifieri5306 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesPolyporalesPhanerochaetaceaePhanerochaete

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 242 PublicationsAdd BLAST24
ChainiPRO_000002378025 – 382Manganese peroxidase H4Add BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 39PROSITE-ProRule annotation1 Publication
Disulfide bondi38 ↔ 313PROSITE-ProRule annotation1 Publication
Disulfide bondi57 ↔ 141PROSITE-ProRule annotation1 Publication
Glycosylationi100N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi155N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi241N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi277 ↔ 344PROSITE-ProRule annotation1 Publication
Disulfide bondi366 ↔ 373PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Inductioni

During wound-healing and by factors which induce suberization.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP19136
SMRiP19136
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Ligninase subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IZ9A Eukaryota
ENOG410YEPY LUCA
OMAiYEMTAKF

Family and domain databases

CDDicd00692 ligninase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR001621 Ligninase
IPR024589 Ligninase_C
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PF11895 Peroxidase_ext, 1 hit
PRINTSiPR00462 LIGNINASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P19136-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAFGSLLAFV ALAAITRAAP TAESAVCPDG TRVTNAACCA FIPLAQDLQE
60 70 80 90 100
TLFQGDCGED AHEVIRLTFH DAIAISQSLG PQAGGGADGS MLHFPTIEPN
110 120 130 140 150
FSANSGIDDS VNNLLPFMQK HDTISAADLV QFAGAVALSN CPGAPRLEFM
160 170 180 190 200
AGRPNTTIPA VEGLIPEPQD SVTKILQRFE DAGNFSPFEV VSLLASHTVA
210 220 230 240 250
RADKVDETID AAPFDSTPFT FDTQVFLEVL LKGTGFPGSN NNTGEVMSPL
260 270 280 290 300
PLGSGSDTGE MRLQSDFALA RDERTACFWQ SFVNEQEFMA ASFKAAMAKL
310 320 330 340 350
AILGHSRSSL IDCSDVVPVP KPAVNKPATF PATKGPKDLD TLTCKALKFP
360 370 380
TLTSDPGATE TLIPHCSNGG MSCPGVQFDG PA
Length:382
Mass (Da):40,115
Last modified:November 1, 1990 - v1
Checksum:i75E2A6B762867E3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04980 mRNA Translation: AAA33746.1
PIRiA32630

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04980 mRNA Translation: AAA33746.1
PIRiA32630

3D structure databases

ProteinModelPortaliP19136
SMRiP19136
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA2 Auxiliary Activities 2
mycoCLAPiMPO2D_PHACH
PeroxiBasei2383 PcMnP02_RP78

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410IZ9A Eukaryota
ENOG410YEPY LUCA
OMAiYEMTAKF

Family and domain databases

CDDicd00692 ligninase, 1 hit
InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR001621 Ligninase
IPR024589 Ligninase_C
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PF11895 Peroxidase_ext, 1 hit
PRINTSiPR00462 LIGNINASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPEM4_PHACH
AccessioniPrimary (citable) accession number: P19136
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: November 7, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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