UniProtKB - P19109 (DDX17_DROME)
Protein
ATP-dependent RNA helicase p62
Gene
Rm62
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
As an RNA helicase, unwinds RNA and alters RNA structures through ATP binding and hydrolysis. Involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, rRNA processing and miRNA processing, as well as transcription regulation (By similarity) (PubMed:12368261). Plays a role in innate immunity. Specifically restricts bunyavirus infection, including Rift Valley fever virus (RVFV) or La Crosse virus (LACV), but not vesicular stomatitis virus (VSV), in an interferon- and DROSHA-independent manner (PubMed:25126784).By similarity2 Publications
Catalytic activityi
- EC:3.6.4.13By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 325 – 332 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- mRNA binding Source: FlyBase
- RNA binding Source: GO_Central
- RNA helicase activity Source: GO_Central
GO - Biological processi
- defense response to virus Source: UniProtKB-KW
- mRNA splicing, via spliceosome Source: FlyBase
- regulation of alternative mRNA splicing, via spliceosome Source: FlyBase
- regulation of defense response to virus Source: FlyBase
- regulation of translation Source: UniProtKB-KW
- RNA interference Source: FlyBase
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Antiviral defense, Immunity, mRNA processing, mRNA splicing, RNA-mediated gene silencing, Translation regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rm62 Synonyms:Dmp68, p62 ORF Names:CG10279 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0003261, Rm62 |
Subcellular locationi
Cytosol
- cytosol Source: UniProtKB-SubCell
Nucleus
- catalytic step 2 spliceosome Source: FlyBase
- nucleolus Source: UniProtKB-SubCell
- nucleus Source: FlyBase
- precatalytic spliceosome Source: FlyBase
Other locations
- cytoplasm Source: FlyBase
- polytene chromosome Source: FlyBase
- polytene chromosome puff Source: FlyBase
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
No phenotype under normal conditions. Upon infection with Rift Valley fever virus (RVFV) or La Crosse virus (LACV), knockdown flies exhibit increased mortality. No phenotype upon infection with other viruses, including vesicular stomatitis virus (VSV), Sindbis virus (SINV), nor Drosophila C virus (DCV).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000055007 | 1 – 719 | ATP-dependent RNA helicase p62Add BLAST | 719 |
Proteomic databases
PaxDbi | P19109 |
PRIDEi | P19109 |
Expressioni
Gene expression databases
Bgeei | FBgn0003261, Expressed in embryo and 38 other tissues |
ExpressionAtlasi | P19109, baseline and differential |
Genevisiblei | P19109, DM |
Interactioni
Subunit structurei
Interacts with Fmr1 to form the RNA-induced silencing complex (RISC), a ribonucleoprotein (RNP) complex involved in translation regulation, other components of the complex are RpL5, RpL11, AGO2 and Dcr-1.
1 PublicationBinary interactionsi
P19109
With | #Exp. | IntAct |
---|---|---|
Fmr1 [Q9NFU0] | 4 | EBI-200734,EBI-422631 |
Protein-protein interaction databases
BioGRIDi | 65943, 43 interactors |
DIPi | DIP-17867N |
IntActi | P19109, 36 interactors |
MINTi | P19109 |
STRINGi | 7227.FBpp0078301 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 312 – 487 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 176 | |
Domaini | 519 – 664 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 146 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 281 – 309 | Q motifAdd BLAST | 29 | |
Motifi | 435 – 438 | DEAD box | 4 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 153 – 225 | Gly-richAdd BLAST | 73 | |
Compositional biasi | 671 – 713 | Gly-richAdd BLAST | 43 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0331, Eukaryota |
GeneTreei | ENSGT00940000170749 |
HOGENOMi | CLU_003041_16_7_1 |
InParanoidi | P19109 |
PhylomeDBi | P19109 |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform A (identifier: P19109-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLKLVQYIAP RVGGATPRPT ACGWGNLLLI SPRSGASSEK CITQRRHFLF
60 70 80 90 100
SSASSSGTFA SSSSLCTEQR QQFHGSRRNR ETILFPSTYS SLQAQSQRAF
110 120 130 140 150
RDSSKPDSDD YVDSIPKAEQ RTRTRKSLFN DPDERTEEIK IEGVMAPHDR
160 170 180 190 200
DFGHSGRGGR GGDRGGDDRR GGGGGGNRFG GGGGGGDYHG IRNGRVEKRR
210 220 230 240 250
DDRGGGNRFG GGGGFGDRRG GGGGGSQDLP MRPVDFSNLA PFKKNFYQEH
260 270 280 290 300
PNVANRSPYE VQRYREEQEI TVRGQVPNPI QDFSEVHLPD YVMKEIRRQG
310 320 330 340 350
YKAPTAIQAQ GWPIAMSGSN FVGIAKTGSG KTLGYILPAI VHINNQQPLQ
360 370 380 390 400
RGDGPIALVL APTRELAQQI QQVATEFGSS SYVRNTCVFG GAPKGGQMRD
410 420 430 440 450
LQRGCEIVIA TPGRLIDFLS AGSTNLKRCT YLVLDEADRM LDMGFEPQIR
460 470 480 490 500
KIVSQIRPDR QTLMWSATWP KEVKQLAEDF LGNYIQINIG SLELSANHNI
510 520 530 540 550
RQVVDVCDEF SKEEKLKTLL SDIYDTSESP GKIIIFVETK RRVDNLVRFI
560 570 580 590 600
RSFGVRCGAI HGDKSQSERD FVLREFRSGK SNILVATDVA ARGLDVDGIK
610 620 630 640 650
YVINFDYPQN SEDYIHRIGR TGRSNTKGTS FAFFTKNNAK QAKALVDVLR
660 670 680 690 700
EANQEINPAL ENLARNSRYD GGGGRSRYGG GGGGGRFGGG GFKKGSLSNG
710
RGFGGGGGGG GEGRHSRFD
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC7LAE4 | C7LAE4_DROME | RNA helicase | Rm62 4136, Dmel\CG10279, Dmp68, dmP68, DmRH8 | 578 | Annotation score: | ||
E1JJ68 | E1JJ68_DROME | RNA helicase | Rm62 4136, Dmel\CG10279, Dmp68, dmP68, DmRH8 | 575 | Annotation score: | ||
A0A0B4K624 | A0A0B4K624_DROME | RNA helicase | Rm62 4136, Dmel\CG10279, Dmp68, dmP68, DmRH8 | 458 | Annotation score: | ||
A0A0B4K6A3 | A0A0B4K6A3_DROME | RNA helicase | Rm62 4136, Dmel\CG10279, Dmp68, dmP68, DmRH8 | 473 | Annotation score: | ||
A0A0B4K6K1 | A0A0B4K6K1_DROME | RNA helicase | Rm62 4136, Dmel\CG10279, Dmp68, dmP68, DmRH8 | 478 | Annotation score: | ||
A0A0B4K6T7 | A0A0B4K6T7_DROME | RNA helicase | Rm62 4136, Dmel\CG10279, Dmp68, dmP68, DmRH8 | 485 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 195 | R → A in CAA37037 (PubMed:2170937).Curated | 1 | |
Sequence conflicti | 675 | R → P in CAA37037 (PubMed:2170937).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_007414 | 1 – 144 | Missing in isoform D. 2 PublicationsAdd BLAST | 144 | |
Alternative sequenceiVSP_007413 | 1 – 141 | Missing in isoform C and isoform E. 1 PublicationAdd BLAST | 141 | |
Alternative sequenceiVSP_007416 | 142 – 145 | EGVM → MRAK in isoform E. Curated | 4 | |
Alternative sequenceiVSP_007415 | 142 – 144 | EGV → MMM in isoform C. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52846 mRNA Translation: CAA37037.1 AE014297 Genomic DNA Translation: AAF51926.2 AE014297 Genomic DNA Translation: AAF51927.2 AE014297 Genomic DNA Translation: AAG22212.1 AE014297 Genomic DNA Translation: AAG22213.2 AE014297 Genomic DNA Translation: AAN14331.1 AE014297 Genomic DNA Translation: AAN14332.1 BT001716 mRNA Translation: AAN71471.1 BT011476 mRNA Translation: AAR99134.1 BT015209 mRNA Translation: AAT94438.1 |
PIRi | S11485 |
RefSeqi | NP_001163528.1, NM_001170057.1 [P19109-3] NP_001189182.1, NM_001202253.2 [P19109-4] NP_524243.2, NM_079519.2 [P19109-1] NP_731031.1, NM_169118.1 [P19109-3] NP_731032.1, NM_169119.3 [P19109-4] NP_731033.1, NM_169120.1 [P19109-2] NP_731034.1, NM_169121.2 [P19109-2] NP_731035.2, NM_169122.1 [P19109-2] |
Genome annotation databases
EnsemblMetazoai | FBtr0078649; FBpp0078298; FBgn0003261 [P19109-2] FBtr0078650; FBpp0078299; FBgn0003261 [P19109-3] FBtr0078651; FBpp0078300; FBgn0003261 [P19109-2] FBtr0078652; FBpp0078301; FBgn0003261 [P19109-1] FBtr0078653; FBpp0078302; FBgn0003261 [P19109-4] FBtr0078654; FBpp0078303; FBgn0003261 [P19109-2] FBtr0301947; FBpp0291159; FBgn0003261 [P19109-3] FBtr0302597; FBpp0291753; FBgn0003261 [P19109-4] |
GeneIDi | 40739 |
KEGGi | dme:Dmel_CG10279 |
UCSCi | CG10279-RC, d. melanogaster |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52846 mRNA Translation: CAA37037.1 AE014297 Genomic DNA Translation: AAF51926.2 AE014297 Genomic DNA Translation: AAF51927.2 AE014297 Genomic DNA Translation: AAG22212.1 AE014297 Genomic DNA Translation: AAG22213.2 AE014297 Genomic DNA Translation: AAN14331.1 AE014297 Genomic DNA Translation: AAN14332.1 BT001716 mRNA Translation: AAN71471.1 BT011476 mRNA Translation: AAR99134.1 BT015209 mRNA Translation: AAT94438.1 |
PIRi | S11485 |
RefSeqi | NP_001163528.1, NM_001170057.1 [P19109-3] NP_001189182.1, NM_001202253.2 [P19109-4] NP_524243.2, NM_079519.2 [P19109-1] NP_731031.1, NM_169118.1 [P19109-3] NP_731032.1, NM_169119.3 [P19109-4] NP_731033.1, NM_169120.1 [P19109-2] NP_731034.1, NM_169121.2 [P19109-2] NP_731035.2, NM_169122.1 [P19109-2] |
3D structure databases
SMRi | P19109 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 65943, 43 interactors |
DIPi | DIP-17867N |
IntActi | P19109, 36 interactors |
MINTi | P19109 |
STRINGi | 7227.FBpp0078301 |
Proteomic databases
PaxDbi | P19109 |
PRIDEi | P19109 |
Genome annotation databases
EnsemblMetazoai | FBtr0078649; FBpp0078298; FBgn0003261 [P19109-2] FBtr0078650; FBpp0078299; FBgn0003261 [P19109-3] FBtr0078651; FBpp0078300; FBgn0003261 [P19109-2] FBtr0078652; FBpp0078301; FBgn0003261 [P19109-1] FBtr0078653; FBpp0078302; FBgn0003261 [P19109-4] FBtr0078654; FBpp0078303; FBgn0003261 [P19109-2] FBtr0301947; FBpp0291159; FBgn0003261 [P19109-3] FBtr0302597; FBpp0291753; FBgn0003261 [P19109-4] |
GeneIDi | 40739 |
KEGGi | dme:Dmel_CG10279 |
UCSCi | CG10279-RC, d. melanogaster |
Organism-specific databases
CTDi | 40739 |
FlyBasei | FBgn0003261, Rm62 |
Phylogenomic databases
eggNOGi | KOG0331, Eukaryota |
GeneTreei | ENSGT00940000170749 |
HOGENOMi | CLU_003041_16_7_1 |
InParanoidi | P19109 |
PhylomeDBi | P19109 |
Miscellaneous databases
BioGRID-ORCSi | 40739, 1 hit in 3 CRISPR screens |
ChiTaRSi | Rm62, fly |
GenomeRNAii | 40739 |
PROi | PR:P19109 |
Gene expression databases
Bgeei | FBgn0003261, Expressed in embryo and 38 other tissues |
ExpressionAtlasi | P19109, baseline and differential |
Genevisiblei | P19109, DM |
Family and domain databases
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DDX17_DROME | |
Accessioni | P19109Primary (citable) accession number: P19109 Secondary accession number(s): A4V2H1 Q9VNK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | May 16, 2003 | |
Last modified: | December 2, 2020 | |
This is version 195 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - SIMILARITY comments
Index of protein domains and families