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Protein

Fatty acid synthase subunit alpha

Gene

FAS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase. This subunit coordinates the binding of the six beta subunits to the enzyme complex.

Miscellaneous

Present with 17000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO2 + 2n NADP+.
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Activity regulationi

Inhibited by cerulenin by covalent binding to active site of the ketoacyl synthase (KS) region.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1305For beta-ketoacyl synthase activity1
Metal bindingi1772Magnesium1
Metal bindingi1773Magnesium; via carbonyl oxygen1
Metal bindingi1774Magnesium1
Binding sitei1798Acetyl-CoA1 Publication1
Binding sitei1808Acetyl-CoA1 Publication1
Metal bindingi1872Magnesium1
Metal bindingi1873Magnesium; via carbonyl oxygen1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMultifunctional enzyme, Oxidoreductase, Transferase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandMagnesium, Metal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciMetaCyc:YPL231W-MONOMER
YEAST:YPL231W-MONOMER
SABIO-RKiP19097

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid synthase subunit alpha (EC:2.3.1.86)
Including the following 3 domains:
Acyl carrier
3-oxoacyl-[acyl-carrier-protein] reductase (EC:1.1.1.100)
Alternative name(s):
Beta-ketoacyl reductase
3-oxoacyl-[acyl-carrier-protein] synthase (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl synthase
Gene namesi
Name:FAS2
Ordered Locus Names:YPL231W
ORF Names:P1409
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL231W
SGDiS000006152 FAS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1250G → S: Cerulenin-resistance. 1 Publication1
Mutagenesisi1769V → D: Does not affect oligomerization; when associated with S-1771 and L-1773 or S-1771; L-1773; S-1879 and E-1881. 1 Publication1
Mutagenesisi1770G → D: Loss of transferase activity. 1 Publication1
Mutagenesisi1771V → S: Does not affect oligomerization but lacks transferase activity; when associated with D-1769 and L-1773 or D-1769; L-1773; S-1879 and E-1881. 1 Publication1
Mutagenesisi1772D → S: Loss of transferase activity; when associated with S-1774. 1 Publication1
Mutagenesisi1773V → L: Does not affect oligomerization but lacks transferase activity; when associated with D-1769 and S-1771 or D-1769; S-1771; S-1879 and E-1881. 1 Publication1
Mutagenesisi1774E → S: Loss of transferase activity; when associated with S-1772. 1 Publication1
Mutagenesisi1841R → A: Loss off transferase activity. 1 Publication1
Mutagenesisi1879V → S: Does not affect oligomerization but lacks transferase activity; when associated with D-1769; S-1771; L-1773 and E-1881. 1 Publication1
Mutagenesisi1881V → E: Does not affect oligomerization but lacks transferase activity; when associated with D-1769; S-1771; L-1773 and S-1879. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001802871 – 1887Fatty acid synthase subunit alphaAdd BLAST1887

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki37Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei50PhosphoserineCombined sources1
Modified residuei180O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1 Publication1
Modified residuei523PhosphoserineCombined sources1
Modified residuei958PhosphoserineCombined sources1
Modified residuei1440PhosphoserineCombined sources1

Post-translational modificationi

4'-phosphopantetheine is transferred from CoA to a specific serine of the Acyl carrier domain by the C-terminal PPT domain. This modification is essential for activity because fatty acids are bound in thioester linkage to the sulfhydryl of the prosthetic group.

Keywords - PTMi

Isopeptide bond, Phosphopantetheine, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP19097
PaxDbiP19097
PRIDEiP19097
TopDownProteomicsiP19097

PTM databases

iPTMnetiP19097

Interactioni

Subunit structurei

[Alpha6beta6] hexamers of two multifunctional subunits (alpha and beta).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
FAS1P071498EBI-6806,EBI-6795

Protein-protein interaction databases

BioGridi35931, 377 interactors
ComplexPortaliCPX-1162 Fatty-acyl-CoA synthase
DIPiDIP-960N
IntActiP19097, 19 interactors
MINTiP19097
STRINGi4932.YPL231W

Structurei

Secondary structure

11887
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP19097
SMRiP19097
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP19097

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 220CarrierPROSITE-ProRule annotationAdd BLAST76

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni675 – 874Beta-ketoacyl reductaseAdd BLAST200
Regioni1149 – 1363Beta-ketoacyl synthaseAdd BLAST215
Regioni1772 – 1774Acetyl-CoA binding3
Regioni1817 – 1833Acetyl-CoA bindingAdd BLAST17
Regioni1841 – 1844Acetyl-CoA binding4
Regioni1871 – 1873Acetyl-CoA binding3

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000177974
InParanoidiP19097
KOiK00667
OMAiMMDPAAI
OrthoDBiEOG092C010Q

Family and domain databases

Gene3Di3.40.47.10, 3 hits
3.90.470.20, 1 hit
HAMAPiMF_00101 AcpS, 1 hit
InterProiView protein in InterPro
IPR008278 4-PPantetheinyl_Trfase_dom
IPR037143 4-PPantetheinyl_Trfase_dom_sf
IPR002582 ACPS
IPR016035 Acyl_Trfase/lysoPLipase
IPR026025 FAS_alpha_yeast
IPR018201 Ketoacyl_synth_AS
IPR014031 Ketoacyl_synth_C
IPR014030 Ketoacyl_synth_N
IPR036291 NAD(P)-bd_dom_sf
IPR009081 PP-bd_ACP
IPR004568 Ppantetheine-prot_Trfase_dom
IPR016039 Thiolase-like
PfamiView protein in Pfam
PF01648 ACPS, 1 hit
PF00109 ketoacyl-synt, 1 hit
PF02801 Ketoacyl-synt_C, 1 hit
PIRSFiPIRSF000454 FAS_yeast_alpha, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004282 PPantethiene-prot_Trfase, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52151 SSF52151, 1 hit
SSF53901 SSF53901, 4 hits
SSF56214 SSF56214, 1 hit
TIGRFAMsiTIGR00556 pantethn_trn, 1 hit
PROSITEiView protein in PROSITE
PS00606 B_KETOACYL_SYNTHASE, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit

Sequencei

Sequence statusi: Complete.

P19097-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKPEVEQELA HILLTELLAY QFASPVRWIE TQDVFLKDFN TERVVEIGPS
60 70 80 90 100
PTLAGMAQRT LKNKYESYDA ALSLHREILC YSKDAKEIYY TPDPSELAAK
110 120 130 140 150
EEPAKEEAPA PTPAASAPAP AAAAPAPVAA AAPAAAAAEI ADEPVKASLL
160 170 180 190 200
LHVLVAHKLK KSLDSIPMSK TIKDLVGGKS TVQNEILGDL GKEFGTTPEK
210 220 230 240 250
PEETPLEELA ETFQDTFSGA LGKQSSSLLS RLISSKMPGG FTITVARKYL
260 270 280 290 300
QTRWGLPSGR QDGVLLVALS NEPAARLGSE ADAKAFLDSM AQKYASIVGV
310 320 330 340 350
DLSSAASASG AAGAGAAAGA AMIDAGALEE ITKDHKVLAR QQLQVLARYL
360 370 380 390 400
KMDLDNGERK FLKEKDTVAE LQAQLDYLNA ELGEFFVNGV ATSFSRKKAR
410 420 430 440 450
TFDSSWNWAK QSLLSLYFEI IHGVLKNVDR EVVSEAINIM NRSNDALIKF
460 470 480 490 500
MEYHISNTDE TKGENYQLVK TLGEQLIENC KQVLDVDPVY KDVAKPTGPK
510 520 530 540 550
TAIDKNGNIT YSEEPREKVR KLSQYVQEMA LGGPITKESQ PTIEEDLTRV
560 570 580 590 600
YKAISAQADK QDISSSTRVE FEKLYSDLMK FLESSKEIDP SQTTQLAGMD
610 620 630 640 650
VEDALDKDST KEVASLPNKS TISKTVSSTI PRETIPFLHL RKKTPAGDWK
660 670 680 690 700
YDRQLSSLFL DGLEKAAFNG VTFKDKYVLI TGAGKGSIGA EVLQGLLQGG
710 720 730 740 750
AKVVVTTSRF SKQVTDYYQS IYAKYGAKGS TLIVVPFNQG SKQDVEALIE
760 770 780 790 800
FIYDTEKNGG LGWDLDAIIP FAAIPEQGIE LEHIDSKSEF AHRIMLTNIL
810 820 830 840 850
RMMGCVKKQK SARGIETRPA QVILPMSPNH GTFGGDGMYS ESKLSLETLF
860 870 880 890 900
NRWHSESWAN QLTVCGAIIG WTRGTGLMSA NNIIAEGIEK MGVRTFSQKE
910 920 930 940 950
MAFNLLGLLT PEVVELCQKS PVMADLNGGL QFVPELKEFT AKLRKELVET
960 970 980 990 1000
SEVRKAVSIE TALEHKVVNG NSADAAYAQV EIQPRANIQL DFPELKPYKQ
1010 1020 1030 1040 1050
VKQIAPAELE GLLDLERVIV VTGFAEVGPW GSARTRWEME AFGEFSLEGC
1060 1070 1080 1090 1100
VEMAWIMGFI SYHNGNLKGR PYTGWVDSKT KEPVDDKDVK AKYETSILEH
1110 1120 1130 1140 1150
SGIRLIEPEL FNGYNPEKKE MIQEVIVEED LEPFEASKET AEQFKHQHGD
1160 1170 1180 1190 1200
KVDIFEIPET GEYSVKLLKG ATLYIPKALR FDRLVAGQIP TGWNAKTYGI
1210 1220 1230 1240 1250
SDDIISQVDP ITLFVLVSVV EAFIASGITD PYEMYKYVHV SEVGNCSGSG
1260 1270 1280 1290 1300
MGGVSALRGM FKDRFKDEPV QNDILQESFI NTMSAWVNML LISSSGPIKT
1310 1320 1330 1340 1350
PVGACATSVE SVDIGVETIL SGKARICIVG GYDDFQEEGS FEFGNMKATS
1360 1370 1380 1390 1400
NTLEEFEHGR TPAEMSRPAT TTRNGFMEAQ GAGIQIIMQA DLALKMGVPI
1410 1420 1430 1440 1450
YGIVAMAATA TDKIGRSVPA PGKGILTTAR EHHSSVKYAS PNLNMKYRKR
1460 1470 1480 1490 1500
QLVTREAQIK DWVENELEAL KLEAEEIPSE DQNEFLLERT REIHNEAESQ
1510 1520 1530 1540 1550
LRAAQQQWGN DFYKRDPRIA PLRGALATYG LTIDDLGVAS FHGTSTKAND
1560 1570 1580 1590 1600
KNESATINEM MKHLGRSEGN PVIGVFQKFL TGHPKGAAGA WMMNGALQIL
1610 1620 1630 1640 1650
NSGIIPGNRN ADNVDKILEQ FEYVLYPSKT LKTDGVRAVS ITSFGFGQKG
1660 1670 1680 1690 1700
GQAIVVHPDY LYGAITEDRY NEYVAKVSAR EKSAYKFFHN GMIYNKLFVS
1710 1720 1730 1740 1750
KEHAPYTDEL EEDVYLDPLA RVSKDKKSGS LTFNSKNIQS KDSYINANTI
1760 1770 1780 1790 1800
ETAKMIENMT KEKVSNGGVG VDVELITSIN VENDTFIERN FTPQEIEYCS
1810 1820 1830 1840 1850
AQPSVQSSFA GTWSAKEAVF KSLGVKSLGG GAALKDIEIV RVNKNAPAVE
1860 1870 1880
LHGNAKKAAE EAGVTDVKVS ISHDDLQAVA VAVSTKK
Length:1,887
Mass (Da):206,947
Last modified:May 15, 2002 - v2
Checksum:i08B872734CF3AEEA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti310G → GTTGTGG in AAA34601 (PubMed:2900835).Curated1
Sequence conflicti594T → I in AAA34601 (PubMed:2900835).Curated1
Sequence conflicti941 – 1019AKLRK…LERVI → CLNCVKSWLKLLKLERQFPS KLLWSIRLSMAIALMLHMLK SKFNQELTFNWTSQNRNHTN RLNKLLPLSLRVCWIWKELF in AAA34601 (PubMed:2900835).CuratedAdd BLAST79
Sequence conflicti1036 – 1041RWEMEA → KMGNGS in AAA34601 (PubMed:2900835).Curated6
Sequence conflicti1408A → S in AAA34601 (PubMed:2900835).Curated1
Sequence conflicti1671N → T in AAA34601 (PubMed:2900835).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03936 Genomic DNA Translation: AAA34601.1
X76890 Genomic DNA Translation: CAA54218.1
X94561 Genomic DNA Translation: CAA64256.1
Z73586 Genomic DNA Translation: CAA97947.1
Z73587 Genomic DNA Translation: CAA97948.1
BK006949 Genomic DNA Translation: DAA11205.1
PIRiS61703
RefSeqiNP_015093.1, NM_001184045.1

Genome annotation databases

EnsemblFungiiYPL231W; YPL231W; YPL231W
GeneIDi855845
KEGGisce:YPL231W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03936 Genomic DNA Translation: AAA34601.1
X76890 Genomic DNA Translation: CAA54218.1
X94561 Genomic DNA Translation: CAA64256.1
Z73586 Genomic DNA Translation: CAA97947.1
Z73587 Genomic DNA Translation: CAA97948.1
BK006949 Genomic DNA Translation: DAA11205.1
PIRiS61703
RefSeqiNP_015093.1, NM_001184045.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ML8NMR-A138-302[»]
2PFFX-ray4.00A/D/G671-1744[»]
2UV8X-ray3.10A/B/C1-1887[»]
2VKZX-ray4.00A/B/C1-1887[»]
2WASX-ray1.90A/B/C/D/E/F1766-1887[»]
2WATX-ray2.20A/B/C/D/E/F1766-1887[»]
3HMJX-ray4.00A/B/C1-1887[»]
ProteinModelPortaliP19097
SMRiP19097
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35931, 377 interactors
ComplexPortaliCPX-1162 Fatty-acyl-CoA synthase
DIPiDIP-960N
IntActiP19097, 19 interactors
MINTiP19097
STRINGi4932.YPL231W

PTM databases

iPTMnetiP19097

Proteomic databases

MaxQBiP19097
PaxDbiP19097
PRIDEiP19097
TopDownProteomicsiP19097

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL231W; YPL231W; YPL231W
GeneIDi855845
KEGGisce:YPL231W

Organism-specific databases

EuPathDBiFungiDB:YPL231W
SGDiS000006152 FAS2

Phylogenomic databases

HOGENOMiHOG000177974
InParanoidiP19097
KOiK00667
OMAiMMDPAAI
OrthoDBiEOG092C010Q

Enzyme and pathway databases

BioCyciMetaCyc:YPL231W-MONOMER
YEAST:YPL231W-MONOMER
SABIO-RKiP19097

Miscellaneous databases

EvolutionaryTraceiP19097
PROiPR:P19097

Family and domain databases

Gene3Di3.40.47.10, 3 hits
3.90.470.20, 1 hit
HAMAPiMF_00101 AcpS, 1 hit
InterProiView protein in InterPro
IPR008278 4-PPantetheinyl_Trfase_dom
IPR037143 4-PPantetheinyl_Trfase_dom_sf
IPR002582 ACPS
IPR016035 Acyl_Trfase/lysoPLipase
IPR026025 FAS_alpha_yeast
IPR018201 Ketoacyl_synth_AS
IPR014031 Ketoacyl_synth_C
IPR014030 Ketoacyl_synth_N
IPR036291 NAD(P)-bd_dom_sf
IPR009081 PP-bd_ACP
IPR004568 Ppantetheine-prot_Trfase_dom
IPR016039 Thiolase-like
PfamiView protein in Pfam
PF01648 ACPS, 1 hit
PF00109 ketoacyl-synt, 1 hit
PF02801 Ketoacyl-synt_C, 1 hit
PIRSFiPIRSF000454 FAS_yeast_alpha, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD004282 PPantethiene-prot_Trfase, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52151 SSF52151, 1 hit
SSF53901 SSF53901, 4 hits
SSF56214 SSF56214, 1 hit
TIGRFAMsiTIGR00556 pantethn_trn, 1 hit
PROSITEiView protein in PROSITE
PS00606 B_KETOACYL_SYNTHASE, 1 hit
PS50075 CARRIER, 1 hit
PS00012 PHOSPHOPANTETHEINE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFAS2_YEAST
AccessioniPrimary (citable) accession number: P19097
Secondary accession number(s): D6W3D9, Q12533
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: May 15, 2002
Last modified: October 10, 2018
This is version 200 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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