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UniProtKB - P19080 (AROH_BACSU)
Protein
Chorismate mutase AroH
Gene
aroH
Organism
Bacillus subtilis (strain 168)
Status
Functioni
Catalyzes the Claisen rearrangement of chorismate to prephenate. Probably involved in the aromatic amino acid biosynthesis.
1 PublicationMiscellaneous
This enzyme is monofunctional, and its activity is unaffected by the end-product aromatic amino acids.
Catalytic activityi
Kineticsi
- KM=67 µM for chorismate (at pH 7.5 and at 30 degrees Celsius)1 Publication
- KM=87 µM for chorismate (at pH 7.5 and at 30 degrees Celsius)1 Publication
- KM=100 µM for chorismate (at pH 7.5 and at 30 degrees Celsius)1 Publication
: prephenate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes prephenate from chorismate. This subpathway is part of the pathway prephenate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes prephenate from chorismate, the pathway prephenate biosynthesis and in Metabolic intermediate biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 7 | PrephenateCombined sources1 Publication | 1 | |
Binding sitei | 90 | PrephenateCombined sources1 Publication | 1 | |
Binding sitei | 108 | PrephenateCombined sources1 Publication | 1 |
GO - Molecular functioni
- chorismate mutase activity Source: UniProtKB
GO - Biological processi
- aromatic amino acid family biosynthetic process Source: UniProtKB-KW
- cellular amino acid biosynthetic process Source: UniProtKB-KW
- chorismate metabolic process Source: UniProtKB
Keywordsi
Molecular function | Isomerase |
Biological process | Amino-acid biosynthesis, Aromatic amino acid biosynthesis |
Enzyme and pathway databases
BioCyci | BSUB:BSU22690-MONOMER |
BRENDAi | 5.4.99.5, 658 |
SABIO-RKi | P19080 |
UniPathwayi | UPA00120;UER00203 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aroH1 Publication Ordered Locus Names:BSU22690 |
Organismi | Bacillus subtilis (strain 168) |
Taxonomic identifieri | 224308 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | E → A: No chorismate mutase activity. 1 Publication | 1 | |
Mutagenesisi | 90 | R → A: No chorismate mutase activity. 1 Publication | 1 | |
Mutagenesisi | 90 | R → G: 2-fold decrease in affinity and very low decrease in catalytic efficiency. 1 Publication | 1 | |
Mutagenesisi | 90 | R → K: Low decrease in catalytic efficiency and in affinity. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB08648, (1R,3S,5S,8R)-8-Hydroxy-2-oxabicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid DB02153, 3-sulfino-L-alanine DB08427, Prephenic acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119203 | 1 – 127 | Chorismate mutase AroHAdd BLAST | 127 |
Proteomic databases
PaxDbi | P19080 |
PRIDEi | P19080 |
Interactioni
Subunit structurei
Homotrimer.
3 PublicationsProtein-protein interaction databases
STRINGi | 224308.BSU22690 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P19080 |
SMRi | P19080 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P19080 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 121 | Chorismate mutase aroH-typePROSITE-ProRule annotationAdd BLAST | 119 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 74 – 78 | Prephenate bindingCombined sources1 Publication | 5 |
Phylogenomic databases
eggNOGi | COG4401, Bacteria |
InParanoidi | P19080 |
OMAi | CIRVMMT |
PhylomeDBi | P19080 |
Family and domain databases
CDDi | cd02185, AroH, 1 hit |
Gene3Di | 3.30.1330.40, 1 hit |
InterProi | View protein in InterPro IPR008243, Chorismate_mutase_AroH IPR035959, RutC-like_sf |
PANTHERi | PTHR21164, PTHR21164, 1 hit |
Pfami | View protein in Pfam PF07736, CM_1, 1 hit |
PIRSFi | PIRSF005965, Chor_mut_AroH, 1 hit |
SUPFAMi | SSF55298, SSF55298, 1 hit |
TIGRFAMsi | TIGR01796, CM_mono_aroH, 1 hit |
PROSITEi | View protein in PROSITE PS51167, CHORISMATE_MUT_1, 1 hit |
i Sequence
Sequence statusi: Complete.
P19080-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMIRGIRGAT TVERDTEEEI LQKTKQLLEK IIEENHTKPE DVVQMLLSAT
60 70 80 90 100
PDLHAVFPAK AVRELSGWQY VPVTCMQEMD VTGGLKKCIR VMMTVQTDVP
110 120
QDQIRHVYLE KVVVLRPDLS LTKNTEL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | V → A in AAA22249 (PubMed:2105742).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32278 Genomic DNA Translation: AAA22249.1 M80245 Genomic DNA Translation: AAA20861.1 AL009126 Genomic DNA Translation: CAB14185.1 |
PIRi | A33894 |
RefSeqi | NP_390150.1, NC_000964.3 WP_009967606.1, NZ_JNCM01000036.1 |
Genome annotation databases
EnsemblBacteriai | CAB14185; CAB14185; BSU_22690 |
GeneIDi | 939005 |
KEGGi | bsu:BSU22690 |
PATRICi | fig|224308.179.peg.2473 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M32278 Genomic DNA Translation: AAA22249.1 M80245 Genomic DNA Translation: AAA20861.1 AL009126 Genomic DNA Translation: CAB14185.1 |
PIRi | A33894 |
RefSeqi | NP_390150.1, NC_000964.3 WP_009967606.1, NZ_JNCM01000036.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1COM | X-ray | 2.20 | A/B/C/D/E/F/G/H/I/J/K/L | 1-127 | [»] | |
1DBF | X-ray | 1.30 | A/B/C | 1-127 | [»] | |
1FNJ | X-ray | 1.90 | A | 1-127 | [»] | |
1FNK | X-ray | 2.00 | A | 1-127 | [»] | |
2CHS | X-ray | 1.90 | A/B/C/D/E/F/G/H/I/J/K/L | 1-127 | [»] | |
2CHT | X-ray | 2.20 | A/B/C/D/E/F/G/H/I/J/K/L | 1-127 | [»] | |
3ZO8 | X-ray | 1.59 | A/B/C/D/E/F | 1-127 | [»] | |
3ZOP | X-ray | 1.61 | A/B/C/D/E/F | 1-127 | [»] | |
3ZP4 | X-ray | 1.80 | A/B/C/D/E/F | 1-127 | [»] | |
3ZP7 | X-ray | 1.70 | A/B/C/D/E/F | 1-127 | [»] | |
BMRBi | P19080 | |||||
SMRi | P19080 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224308.BSU22690 |
Chemistry databases
DrugBanki | DB08648, (1R,3S,5S,8R)-8-Hydroxy-2-oxabicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid DB02153, 3-sulfino-L-alanine DB08427, Prephenic acid |
Proteomic databases
PaxDbi | P19080 |
PRIDEi | P19080 |
Genome annotation databases
EnsemblBacteriai | CAB14185; CAB14185; BSU_22690 |
GeneIDi | 939005 |
KEGGi | bsu:BSU22690 |
PATRICi | fig|224308.179.peg.2473 |
Phylogenomic databases
eggNOGi | COG4401, Bacteria |
InParanoidi | P19080 |
OMAi | CIRVMMT |
PhylomeDBi | P19080 |
Enzyme and pathway databases
UniPathwayi | UPA00120;UER00203 |
BioCyci | BSUB:BSU22690-MONOMER |
BRENDAi | 5.4.99.5, 658 |
SABIO-RKi | P19080 |
Miscellaneous databases
EvolutionaryTracei | P19080 |
PROi | PR:P19080 |
Family and domain databases
CDDi | cd02185, AroH, 1 hit |
Gene3Di | 3.30.1330.40, 1 hit |
InterProi | View protein in InterPro IPR008243, Chorismate_mutase_AroH IPR035959, RutC-like_sf |
PANTHERi | PTHR21164, PTHR21164, 1 hit |
Pfami | View protein in Pfam PF07736, CM_1, 1 hit |
PIRSFi | PIRSF005965, Chor_mut_AroH, 1 hit |
SUPFAMi | SSF55298, SSF55298, 1 hit |
TIGRFAMsi | TIGR01796, CM_mono_aroH, 1 hit |
PROSITEi | View protein in PROSITE PS51167, CHORISMATE_MUT_1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AROH_BACSU | |
Accessioni | P19080Primary (citable) accession number: P19080 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | October 19, 2002 | |
Last modified: | February 23, 2022 | |
This is version 156 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references