UniProtKB - P19024 (KCNA5_RAT)
Protein
Potassium voltage-gated channel subfamily A member 5
Gene
Kcna5
Organism
Rattus norvegicus (Rat)
Status
Functioni
Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane (PubMed:2361015, PubMed:15618540). Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel (PubMed:15618540). Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation (PubMed:15618540). Homotetrameric channels display rapid activation and slow inactivation. May play a role in regulating the secretion of insulin in normal pancreatic islets (By similarity).By similarity3 Publications
GO - Molecular functioni
- alpha-actinin binding Source: RGD
- delayed rectifier potassium channel activity Source: UniProtKB
- outward rectifier potassium channel activity Source: RGD
- potassium channel inhibitor activity Source: RGD
- protein kinase binding Source: RGD
- scaffold protein binding Source: RGD
- signaling receptor binding Source: RGD
- voltage-gated potassium channel activity Source: RGD
- voltage-gated potassium channel activity involved in atrial cardiac muscle cell action potential repolarization Source: RGD
- voltage-gated potassium channel activity involved in bundle of His cell action potential repolarization Source: RGD
- voltage-gated potassium channel activity involved in SA node cell action potential repolarization Source: RGD
GO - Biological processi
- atrial cardiac muscle cell action potential Source: RGD
- membrane hyperpolarization Source: RGD
- membrane repolarization during atrial cardiac muscle cell action potential Source: RGD
- membrane repolarization during bundle of His cell action potential Source: RGD
- membrane repolarization during SA node cell action potential Source: RGD
- negative regulation of cytosolic calcium ion concentration Source: RGD
- negative regulation of potassium ion transport Source: RGD
- Notch signaling pathway Source: RGD
- positive regulation of G1/S transition of mitotic cell cycle Source: RGD
- positive regulation of myoblast proliferation Source: RGD
- potassium ion export across plasma membrane Source: RGD
- potassium ion homeostasis Source: RGD
- potassium ion transmembrane transport Source: UniProtKB
- potassium ion transport Source: RGD
- protein complex oligomerization Source: RGD
- protein homooligomerization Source: InterPro
- regulation of atrial cardiac muscle cell membrane repolarization Source: RGD
- regulation of heart rate by cardiac conduction Source: RGD
- regulation of membrane potential Source: RGD
- regulation of potassium ion transport Source: RGD
- regulation of vasoconstriction Source: RGD
- response to hydrogen peroxide Source: RGD
- response to hyperoxia Source: RGD
- response to hypoxia Source: RGD
- response to mechanical stimulus Source: RGD
- response to organic substance Source: RGD
Keywordsi
Molecular function | Ion channel, Potassium channel, Voltage-gated channel |
Biological process | Ion transport, Potassium transport, Transport |
Ligand | Potassium |
Enzyme and pathway databases
Reactomei | R-RNO-1296072, Voltage gated Potassium channels |
Names & Taxonomyi
Protein namesi | Recommended name: Potassium voltage-gated channel subfamily A member 5Alternative name(s): RCK7 RK4 Voltage-gated potassium channel subunit Kv1.5 |
Gene namesi | Name:Kcna5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2953, Kcna5 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Golgi apparatus
- Golgi apparatus Source: RGD
Plasma Membrane
- integral component of plasma membrane Source: UniProtKB
- intracellular canaliculus Source: RGD
- plasma membrane Source: RGD
- voltage-gated potassium channel complex Source: UniProtKB
Other locations
- cell surface Source: RGD
- integral component of membrane Source: GO_Central
- intercalated disc Source: RGD
- membrane Source: RGD
- membrane raft Source: RGD
- perinuclear region of cytoplasm Source: RGD
- potassium channel complex Source: UniProtKB
- Z disc Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 238 | CytoplasmicBy similarityAdd BLAST | 238 | |
Transmembranei | 239 – 260 | Helical; Name=Segment S1By similarityAdd BLAST | 22 | |
Topological domaini | 261 – 314 | ExtracellularBy similarityAdd BLAST | 54 | |
Transmembranei | 315 – 336 | Helical; Name=Segment S2By similarityAdd BLAST | 22 | |
Topological domaini | 337 – 347 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 348 – 368 | Helical; Name=Segment S3By similarityAdd BLAST | 21 | |
Topological domaini | 369 – 384 | ExtracellularBy similarityAdd BLAST | 16 | |
Transmembranei | 385 – 405 | Helical; Voltage-sensor; Name=Segment S4By similarityAdd BLAST | 21 | |
Topological domaini | 406 – 420 | CytoplasmicBy similarityAdd BLAST | 15 | |
Transmembranei | 421 – 442 | Helical; Name=Segment S5By similarityAdd BLAST | 22 | |
Topological domaini | 443 – 456 | ExtracellularBy similarityAdd BLAST | 14 | |
Intramembranei | 457 – 468 | Helical; Name=Pore helixBy similarityAdd BLAST | 12 | |
Intramembranei | 469 – 476 | By similarity | 8 | |
Topological domaini | 477 – 483 | ExtracellularBy similarity | 7 | |
Transmembranei | 484 – 512 | Helical; Name=Segment S6By similarityAdd BLAST | 29 | |
Topological domaini | 513 – 602 | CytoplasmicBy similarityAdd BLAST | 90 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 600 – 602 | TDL → AAA: Loss of interaction with DLG1. 1 Publication | 3 |
Chemistry databases
ChEMBLi | CHEMBL4295724 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053989 | 1 – 602 | Potassium voltage-gated channel subfamily A member 5Add BLAST | 602 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 81 | Phosphoserine; by CK2 and PKASequence analysis | 1 | |
Cross-linki | 212 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Lipidationi | 337 | S-palmitoyl cysteineSequence analysis | 1 | |
Cross-linki | 525 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 535 | Phosphoserine; by PKASequence analysis | 1 | |
Modified residuei | 546 | Phosphoserine; by PKASequence analysis | 1 | |
Modified residuei | 569 | Phosphoserine; by PKASequence analysis | 1 |
Keywords - PTMi
Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugationProteomic databases
PaxDbi | P19024 |
PRIDEi | P19024 |
Expressioni
Tissue specificityi
Expressed equally in atrium, ventricle, aorta and skeletal muscle. Weaker expression in brain.1 Publication
Inductioni
Expression regulated by cAMP in a tissue-specific manner. In primary cardiac cells, levels increase by 6-fold, and in GH3 cells, levels decrease 5-6-fold.
Gene expression databases
Bgeei | ENSRNOG00000019719, Expressed in Ammon's horn and 17 other tissues |
Genevisiblei | P19024, RN |
Interactioni
Subunit structurei
Homotetramer and heterotetramer of potassium channel proteins.
Interacts with DLG1, which enhances channel currents (PubMed:11709425).
Forms a ternary complex with DLG1 and CAV3 (PubMed:15277200).
Interacts with KCNAB1 (By similarity).
Interacts with UBE2I (By similarity).
By similarity2 PublicationsGO - Molecular functioni
- alpha-actinin binding Source: RGD
- protein kinase binding Source: RGD
- scaffold protein binding Source: RGD
- signaling receptor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247503, 6 interactors |
CORUMi | P19024 |
IntActi | P19024, 4 interactors |
MINTi | P19024 |
STRINGi | 10116.ENSRNOP00000026691 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 407 – 420 | S4-S5 linkerBy similarityAdd BLAST | 14 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 469 – 474 | Selectivity filterBy similarity | 6 | |
Motifi | 600 – 602 | PDZ-binding | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 373 – 376 | Poly-Gly | 4 |
Domaini
The N-terminus may be important in determining the rate of inactivation of the channel while the C-terminal PDZ-binding motif may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The transmembrane segment S4 functions as voltage-sensor and is characterized by a series of positively charged amino acids at every third position. Channel opening and closing is effected by a conformation change that affects the position and orientation of the voltage-sensor paddle formed by S3 and S4 within the membrane. A transmembrane electric field that is positive inside would push the positively charged S4 segment outwards, thereby opening the pore, while a field that is negative inside would pull the S4 segment inwards and close the pore. Changes in the position and orientation of S4 are then transmitted to the activation gate formed by the inner helix bundle via the S4-S5 linker region.By similarity
Sequence similaritiesi
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.5/KCNA5 sub-subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1545, Eukaryota |
GeneTreei | ENSGT00940000161860 |
HOGENOMi | CLU_011722_4_0_1 |
InParanoidi | P19024 |
OMAi | ADHSPHL |
OrthoDBi | 695337at2759 |
PhylomeDBi | P19024 |
TreeFami | TF313103 |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003972, K_chnl_volt-dep_Kv1 IPR004052, K_chnl_volt-dep_Kv1.5 IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF00520, Ion_trans, 1 hit |
PRINTSi | PR01512, KV15CHANNEL PR01491, KVCHANNEL PR01496, SHAKERCHANEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
i Sequence
Sequence statusi: Complete.
P19024-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEISLVPLEN GSAMTLRGGG EAGASCVQTP RGECGCPPTS GLNNQSKETL
60 70 80 90 100
LRGRTTLEDA NQGGRPLPPM AQELPQPRRL SAEDEEGEGD PGLGTVEEDQ
110 120 130 140 150
APQDAGSLHH QRVLINISGL RFETQLGTLA QFPNTLLGDP AKRLHYFDPL
160 170 180 190 200
RNEYFFDRNR PSFDGILYYY QSGGRLRRPV NVSLDVFADE IRFYQLGDEA
210 220 230 240 250
MERFREDEGF IKEEEKPLPR NEFQRQVWLI FEYPESSGSA RAIAIVSVLV
260 270 280 290 300
ILISIITFCL ETLPEFRDER ELLRHPPVPP QPPAPAPGIN GSVSGALSSG
310 320 330 340 350
PTVAPLLPRT LADPFFIVET TCVIWFTFEL LVRFFACPSK AEFSRNIMNI
360 370 380 390 400
IDVVAIFPYF ITLGTELAEQ QPGGGGQNGQ QAMSLAILRV IRLVRVFRIF
410 420 430 440 450
KLSRHSKGLQ ILGKTLQASM RELGLLIFFL FIGVILFSSA VYFAEADNHG
460 470 480 490 500
SHFSSIPDAF WWAVVTMTTV GYGDMRPITV GGKIVGSLCA IAGVLTIALP
510 520 530 540 550
VPVIVSNFNY FYHRETDHEE QAALKEEQGN QRRESGLDTG GQRKVSCSKA
560 570 580 590 600
SFCKTGGSLE SSDSIRRGSC PLEKCHLKAK SNVDLRRSLY ALCLDTSRET
DL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 553 | C → S no nucleotide entry (PubMed:1705709).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27158 Genomic DNA Translation: AAA41498.1 L23434 Genomic DNA Translation: AAA42337.1 |
PIRi | JH0166 |
RefSeqi | NP_037104.1, NM_012972.1 |
Genome annotation databases
Ensembli | ENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719 |
GeneIDi | 25470 |
KEGGi | rno:25470 |
UCSCi | RGD:2953, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M27158 Genomic DNA Translation: AAA41498.1 L23434 Genomic DNA Translation: AAA42337.1 |
PIRi | JH0166 |
RefSeqi | NP_037104.1, NM_012972.1 |
3D structure databases
SMRi | P19024 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247503, 6 interactors |
CORUMi | P19024 |
IntActi | P19024, 4 interactors |
MINTi | P19024 |
STRINGi | 10116.ENSRNOP00000026691 |
Chemistry databases
ChEMBLi | CHEMBL4295724 |
Proteomic databases
PaxDbi | P19024 |
PRIDEi | P19024 |
Protocols and materials databases
ABCDi | P19024, 1 sequenced antibody |
Genome annotation databases
Ensembli | ENSRNOT00000026691; ENSRNOP00000026691; ENSRNOG00000019719 |
GeneIDi | 25470 |
KEGGi | rno:25470 |
UCSCi | RGD:2953, rat |
Organism-specific databases
CTDi | 3741 |
RGDi | 2953, Kcna5 |
Phylogenomic databases
eggNOGi | KOG1545, Eukaryota |
GeneTreei | ENSGT00940000161860 |
HOGENOMi | CLU_011722_4_0_1 |
InParanoidi | P19024 |
OMAi | ADHSPHL |
OrthoDBi | 695337at2759 |
PhylomeDBi | P19024 |
TreeFami | TF313103 |
Enzyme and pathway databases
Reactomei | R-RNO-1296072, Voltage gated Potassium channels |
Miscellaneous databases
PROi | PR:P19024 |
Gene expression databases
Bgeei | ENSRNOG00000019719, Expressed in Ammon's horn and 17 other tissues |
Genevisiblei | P19024, RN |
Family and domain databases
Gene3Di | 1.20.120.350, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR005821, Ion_trans_dom IPR003968, K_chnl_volt-dep_Kv IPR003972, K_chnl_volt-dep_Kv1 IPR004052, K_chnl_volt-dep_Kv1.5 IPR011333, SKP1/BTB/POZ_sf IPR003131, T1-type_BTB IPR028325, VG_K_chnl IPR027359, Volt_channel_dom_sf |
PANTHERi | PTHR11537, PTHR11537, 1 hit |
Pfami | View protein in Pfam PF02214, BTB_2, 1 hit PF00520, Ion_trans, 1 hit |
PRINTSi | PR01512, KV15CHANNEL PR01491, KVCHANNEL PR01496, SHAKERCHANEL |
SMARTi | View protein in SMART SM00225, BTB, 1 hit |
SUPFAMi | SSF54695, SSF54695, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KCNA5_RAT | |
Accessioni | P19024Primary (citable) accession number: P19024 Secondary accession number(s): Q6LEB7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1990 | |
Last modified: | December 2, 2020 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families