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UniProtKB - P18964 (VSPA_DABSI)
Protein
Factor V activator RVV-V alpha
Gene
N/A
Organism
Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Status
Functioni
Venom serine protease that activates factor V (F5) in a calcium-independent manner. It cleaves the Arg(1545)-Ser(1546) linkage in the human factor V molecule. Induces the coagulation of mammalian plasma.
2 PublicationsMiscellaneous
There are three isoproteins of RVV-V, designated RVV-V alpha, V-beta, and V-gamma.
Has no proteolytic effect on factor VIII, factor XIII, fibrinogen, and prothrombin.1 Publication
Catalytic activityi
- Fully activates human clotting factor V by a single cleavage at the 1545-Trp-Tyr-Leu-Arg-|-Ser-Asn-Asn-Gly-1552 bond. Cattle, but not rabbit, factor V is cleaved, and no other proteins of the clotting system are attacked. Esterase activity is observed on Bz-Arg-OEt and Tos-Arg-OMe, and amidase activity on Phe-pipecolyl-Arg-NHPhNO(2). EC:3.4.21.95
Activity regulationi
Inhibited by D-Phe-Pro-Arg-chloromethyl ketone (FPRCK) (97%), PMSF (76%), and benzamidine (50%). Is not inhibited by BPTI, antithrombin and EDTA.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 43 | Charge relay systemBy similarity | 1 | |
Active sitei | 88 | Charge relay systemBy similarity | 1 | |
Active sitei | 182 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: InterPro
- toxin activity Source: UniProtKB-KW
Keywordsi
Molecular function | Blood coagulation cascade activating toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin |
Enzyme and pathway databases
BRENDAi | 3.4.21.95, 6667 |
Protein family/group databases
MEROPSi | S01.184 |
Names & Taxonomyi
Protein namesi | Recommended name: Factor V activator RVV-V alpha (EC:3.4.21.95)Alternative name(s): Russel's viper venom FV activator alpha Short name: RVV-V alpha Snake venom serine protease Short name: SVSP |
Organismi | Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis) |
Taxonomic identifieri | 343250 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Viperidae › Viperinae › Daboia |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088738 | 1 – 236 | Factor V activator RVV-V alphaAdd BLAST | 236 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 7 ↔ 141 | PROSITE-ProRule annotation | ||
Disulfide bondi | 28 ↔ 44 | PROSITE-ProRule annotation | ||
Disulfide bondi | 76 ↔ 234 | PROSITE-ProRule annotation | ||
Disulfide bondi | 120 ↔ 188 | PROSITE-ProRule annotation | ||
Disulfide bondi | 152 ↔ 167 | PROSITE-ProRule annotation | ||
Disulfide bondi | 178 ↔ 203 | PROSITE-ProRule annotation | ||
Glycosylationi | 229 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinPTM databases
iPTMneti | P18964 |
Expressioni
Tissue specificityi
Expressed by the venom gland.
Interactioni
Subunit structurei
Monomer.
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 227 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 227 |
Sequence similaritiesi
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
i Sequence
Sequence statusi: Complete.
P18964-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
VVGGDECNIN EHPFLVALYT STSSTIHCGG ALINREWVLT AAHCDRRNIR
60 70 80 90 100
IKLGMHSKNI RNEDEQIRVP RGKYFCLNTK FPNGLDKDIM LIRLRRPVTY
110 120 130 140 150
STHIAPVSLP SRSRGVGSRC RIMGWGKIST TEDTYPDVPH CTNIFIVKHK
160 170 180 190 200
WCEPLYPWVP ADSRTLCAGI LKGGRDTCHG DSGGPLICNG QIQGIVAGGS
210 220 230
EPCGQHLKPA VYTKVFDYNN WIQNIIAGNR TVTCPP
Sequence databases
PIRi | A32121 |
Similar proteinsi
Cross-referencesi
Sequence databases
PIRi | A32121 |
3D structure databases
SMRi | P18964 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | S01.184 |
PTM databases
iPTMneti | P18964 |
Enzyme and pathway databases
BRENDAi | 3.4.21.95, 6667 |
Family and domain databases
CDDi | cd00190, Tryp_SPc, 1 hit |
Gene3Di | 2.40.10.10, 2 hits |
InterProi | View protein in InterPro IPR009003, Peptidase_S1_PA IPR043504, Peptidase_S1_PA_chymotrypsin IPR001314, Peptidase_S1A IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER |
Pfami | View protein in Pfam PF00089, Trypsin, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00020, Tryp_SPc, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit |
PROSITEi | View protein in PROSITE PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | VSPA_DABSI | |
Accessioni | P18964Primary (citable) accession number: P18964 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1990 | |
Last modified: | February 23, 2022 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencingDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families