UniProtKB - P18946 (CYB_CHICK)
Protein
Cytochrome b
Gene
MT-CYB
Organism
Gallus gallus (Chicken)
Status
Functioni
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.By similarity
Miscellaneous
Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.By similarity
Caution
The full-length protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.4 Publications
Cofactori
heme4 PublicationsNote: Binds 2 heme groups non-covalently.4 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 84 | Iron 1 (heme b562 axial ligand)PROSITE-ProRule annotationCombined sources4 Publications | 1 | |
Metal bindingi | 98 | Iron 2 (heme b566 axial ligand)Combined sources4 Publications | 1 | |
Metal bindingi | 183 | Iron 1 (heme b562 axial ligand)Combined sources4 Publications | 1 | |
Metal bindingi | 197 | Iron 2 (heme b566 axial ligand)Combined sources4 Publications | 1 | |
Binding sitei | 202 | UbiquinoneCombined sources3 Publications | 1 |
GO - Molecular functioni
- heme binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- ubiquinol-cytochrome-c reductase activity Source: InterPro
GO - Biological processi
- respiratory electron transport chain Source: AgBase
- response to oxidative stress Source: AgBase
Keywordsi
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | Heme, Iron, Metal-binding, Ubiquinone |
Enzyme and pathway databases
Reactomei | R-GGA-611105, Respiratory electron transport |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome bAlternative name(s): Complex III subunit 3 Complex III subunit III Cytochrome b-c1 complex subunit 3 Ubiquinol-cytochrome-c reductase complex cytochrome b subunit |
Gene namesi | Name:MT-CYB Synonyms:COB, CYTB, MTCYB |
Encoded oni | Mitochondrion |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein 4 Publications
Mitochondrion
- integral component of mitochondrial inner membrane Source: Ensembl
- mitochondrial respiratory chain complex III Source: UniProtKB
- mitochondrion Source: AgBase
Other locations
- cytoplasm Source: AgBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 34 – 54 | HelicalCombined sources4 PublicationsAdd BLAST | 21 | |
Transmembranei | 78 – 99 | HelicalCombined sources4 PublicationsAdd BLAST | 22 | |
Transmembranei | 114 – 134 | HelicalCombined sources4 PublicationsAdd BLAST | 21 | |
Transmembranei | 179 – 199 | HelicalCombined sources4 PublicationsAdd BLAST | 21 | |
Transmembranei | 227 – 247 | HelicalCombined sources4 PublicationsAdd BLAST | 21 | |
Transmembranei | 289 – 309 | HelicalCombined sources4 PublicationsAdd BLAST | 21 | |
Transmembranei | 321 – 341 | HelicalCombined sources4 PublicationsAdd BLAST | 21 | |
Transmembranei | 348 – 368 | HelicalCombined sources4 PublicationsAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060776 | 1 – 380 | Cytochrome bAdd BLAST | 380 |
Proteomic databases
PaxDbi | P18946 |
Expressioni
Gene expression databases
Bgeei | ENSGALG00000018360, Expressed in spermatocyte and 14 other tissues |
ExpressionAtlasi | P18946, baseline |
Interactioni
Subunit structurei
The cytochrome bc1 complex contains 11 subunits: 3 respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). This cytochrome bc1 complex then forms a dimer.
4 PublicationsProtein-protein interaction databases
STRINGi | 9031.ENSGALP00000034623 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P18946 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P18946 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome b family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4663, Eukaryota |
GeneTreei | ENSGT00390000017948 |
HOGENOMi | CLU_031114_3_0_1 |
InParanoidi | P18946 |
OMAi | HETGSNN |
OrthoDBi | 1125966at2759 |
PhylomeDBi | P18946 |
TreeFami | TF353088 |
Family and domain databases
CDDi | cd00290, cytochrome_b_C, 1 hit cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit |
InterProi | View protein in InterPro IPR005798, Cyt_b/b6_C IPR036150, Cyt_b/b6_C_sf IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR030689, Cytochrome_b IPR016174, Di-haem_cyt_TM |
Pfami | View protein in Pfam PF00032, Cytochrom_B_C, 1 hit PF00033, Cytochrome_B, 1 hit |
PIRSFi | PIRSF038885, COB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit SSF81648, SSF81648, 1 hit |
PROSITEi | View protein in PROSITE PS51003, CYTB_CTER, 1 hit PS51002, CYTB_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P18946-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPNIRKSHP LLKMINNSLI DLPAPSNISA WWNFGSLLAV CLMTQILTGL
60 70 80 90 100
LLAMHYTADT SLAFSSVAHT CRNVQYGWLI RNLHANGASF FFICIFLHIG
110 120 130 140 150
RGLYYGSYLY KETWNTGVIL LLTLMATAFV GYVLPWGQMS FWGATVITNL
160 170 180 190 200
FSAIPYIGHT LVEWAWGGFS VDNPTLTRFF ALHFLLPFAI AGITIIHLTF
210 220 230 240 250
LHESGSNNPL GISSDSDKIP FHPYYSFKDI LGLTLMLTPF LTLALFSPNL
260 270 280 290 300
LGDPENFTPA NPLVTPPHIK PEWYFLFAYA ILRSIPNKLG GVLALAASVL
310 320 330 340 350
ILFLIPFLHK SKQRTMTFRP LSQTLFWLLV ANLLILTWIG SQPVEHPFII
360 370 380
IGQMASLSYF TILLILFPTI GTLENKMLNY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52392 Genomic DNA Translation: CAA36636.1 L08376 Genomic DNA Translation: AAA18844.1 |
PIRi | S10198 |
RefSeqi | NP_006926.1, NC_001323.1 |
Genome annotation databases
Ensembli | ENSGALT00000053400; ENSGALP00000049893; ENSGALG00000032079 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X52392 Genomic DNA Translation: CAA36636.1 L08376 Genomic DNA Translation: AAA18844.1 |
PIRi | S10198 |
RefSeqi | NP_006926.1, NC_001323.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BCC | X-ray | 3.16 | C | 1-380 | [»] | |
1LLY | model | - | A | 1-380 | [»] | |
2BCC | X-ray | 3.50 | C | 1-380 | [»] | |
3BCC | X-ray | 3.70 | C | 1-380 | [»] | |
3CWB | X-ray | 3.51 | C/P | 1-380 | [»] | |
3H1H | X-ray | 3.16 | C/P | 1-380 | [»] | |
3H1I | X-ray | 3.53 | C/P | 1-380 | [»] | |
3H1J | X-ray | 3.00 | C/P | 1-380 | [»] | |
3H1K | X-ray | 3.48 | C/P | 1-380 | [»] | |
3H1L | X-ray | 3.21 | C/P | 1-380 | [»] | |
3L70 | X-ray | 2.75 | C/P | 1-380 | [»] | |
3L71 | X-ray | 2.84 | C/P | 1-380 | [»] | |
3L72 | X-ray | 3.06 | C/P | 1-380 | [»] | |
3L73 | X-ray | 3.04 | C/P | 1-380 | [»] | |
3L74 | X-ray | 2.76 | C/P | 1-380 | [»] | |
3L75 | X-ray | 2.79 | C/P | 1-380 | [»] | |
3TGU | X-ray | 2.70 | C/P | 1-380 | [»] | |
4U3F | X-ray | 3.23 | C/P | 2-380 | [»] | |
SMRi | P18946 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000034623 |
Proteomic databases
PaxDbi | P18946 |
Genome annotation databases
Ensembli | ENSGALT00000053400; ENSGALP00000049893; ENSGALG00000032079 |
Phylogenomic databases
eggNOGi | KOG4663, Eukaryota |
GeneTreei | ENSGT00390000017948 |
HOGENOMi | CLU_031114_3_0_1 |
InParanoidi | P18946 |
OMAi | HETGSNN |
OrthoDBi | 1125966at2759 |
PhylomeDBi | P18946 |
TreeFami | TF353088 |
Enzyme and pathway databases
Reactomei | R-GGA-611105, Respiratory electron transport |
Miscellaneous databases
EvolutionaryTracei | P18946 |
PROi | PR:P18946 |
Gene expression databases
Bgeei | ENSGALG00000018360, Expressed in spermatocyte and 14 other tissues |
ExpressionAtlasi | P18946, baseline |
Family and domain databases
CDDi | cd00290, cytochrome_b_C, 1 hit cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit |
InterProi | View protein in InterPro IPR005798, Cyt_b/b6_C IPR036150, Cyt_b/b6_C_sf IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR030689, Cytochrome_b IPR016174, Di-haem_cyt_TM |
Pfami | View protein in Pfam PF00032, Cytochrom_B_C, 1 hit PF00033, Cytochrome_B, 1 hit |
PIRSFi | PIRSF038885, COB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit SSF81648, SSF81648, 1 hit |
PROSITEi | View protein in PROSITE PS51003, CYTB_CTER, 1 hit PS51002, CYTB_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYB_CHICK | |
Accessioni | P18946Primary (citable) accession number: P18946 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1990 | |
Last modified: | April 7, 2021 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families