UniProtKB - P18911 (RARG_MOUSE)
Protein
Retinoic acid receptor gamma
Gene
Rarg
Organism
Mus musculus (Mouse)
Status
Functioni
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors (By similarity). Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.By similarity2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 90 – 155 | Nuclear receptorPROSITE-ProRule annotationAdd BLAST | 66 | |
Zinc fingeri | 90 – 110 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 126 – 150 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA binding Source: BHF-UCL
- DNA-binding transcription factor activity Source: BHF-UCL
- nuclear receptor activity Source: MGI
- retinoid X receptor binding Source: BHF-UCL
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: MGI
- sequence-specific double-stranded DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- anterior/posterior pattern specification Source: MGI
- bone development Source: UniProtKB
- bone morphogenesis Source: MGI
- camera-type eye development Source: MGI
- cell differentiation Source: GO_Central
- cellular response to leukemia inhibitory factor Source: MGI
- cellular response to retinoic acid Source: MGI
- chondrocyte development Source: UniProtKB
- embryonic camera-type eye development Source: MGI
- embryonic eye morphogenesis Source: MGI
- embryonic hindlimb morphogenesis Source: MGI
- epithelium development Source: MGI
- face development Source: MGI
- gland development Source: MGI
- glandular epithelial cell development Source: MGI
- growth plate cartilage chondrocyte growth Source: UniProtKB
- growth plate cartilage development Source: MGI
- Harderian gland development Source: MGI
- hormone-mediated signaling pathway Source: GO_Central
- limb development Source: UniProtKB
- multicellular organism growth Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of cartilage development Source: MGI
- negative regulation of cell differentiation Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of chondrocyte differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- neural tube closure Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of gene expression Source: MGI
- positive regulation of programmed cell death Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- prostate gland epithelium morphogenesis Source: MGI
- regulation of gene expression Source: MGI
- regulation of myelination Source: MGI
- regulation of myeloid cell differentiation Source: MGI
- reproductive structure development Source: MGI
- response to retinoic acid Source: MGI
- retina development in camera-type eye Source: MGI
- retinal pigment epithelium development Source: MGI
- retinoic acid receptor signaling pathway Source: MGI
- trachea cartilage development Source: MGI
Keywordsi
Molecular function | DNA-binding, Receptor |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-5362517, Signaling by Retinoic Acid |
Names & Taxonomyi
Protein namesi | Recommended name: Retinoic acid receptor gammaShort name: RAR-gamma Alternative name(s): Nuclear receptor subfamily 1 group B member 3 |
Gene namesi | Name:Rarg Synonyms:Nr1b3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:97858, Rarg |
Subcellular locationi
Nucleus
- nuclear chromatin Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB-SubCell
- transcription regulator complex Source: BHF-UCL
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Rarg and Rarb double null mice exhibit growth retardation 3 weeks after birth. Defects are found in the growth plates with deficiency in cartilage. Growth retardation was noticable in limb sketal elements such as femurs. Early lethality and male sterility due to squamous metaplasia of the seminal vesicles and prostate are also observed. Isoform 2 mutants appear normal. The Rarg and Cyp26b1 double null mutation is able to partially rescue limb skeletal morphology without restoring normal expression of proximo-distal patterning genes.3 Publications
Chemistry databases
ChEMBLi | CHEMBL4177 |
DrugCentrali | P18911 |
GuidetoPHARMACOLOGYi | 592 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000053474 | 1 – 458 | Retinoic acid receptor gammaAdd BLAST | 458 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 34 | Omega-N-methylarginineCombined sources | 1 | |
Cross-linki | 172 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 401 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Methylation, Ubl conjugationProteomic databases
MaxQBi | P18911 |
PaxDbi | P18911 |
PeptideAtlasi | P18911 |
PRIDEi | P18911 |
PTM databases
iPTMneti | P18911 |
PhosphoSitePlusi | P18911 |
Expressioni
Developmental stagei
In 9.5-12.5 dpc embryos, expression throughout limb bud mesenchyme. This expression overlaps with that of CYP26B1. Also strongly expressed in the caudal and craniofacial regions.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000001288, Expressed in lip and 348 other tissues |
ExpressionAtlasi | P18911, baseline and differential |
Genevisiblei | P18911, MM |
Interactioni
Subunit structurei
Homodimer (By similarity). Heterodimer with a RXR molecule (By similarity). Binds DNA preferentially as a RAR/RXR heterodimer (By similarity).
Forms a complex with PUS1 and the SRA1 RNA in the nucleus.
By similarity1 PublicationBinary interactionsi
P18911
With | #Exp. | IntAct |
---|---|---|
Ajuba [Q91XC0] | 2 | EBI-8053650,EBI-1565930 |
GO - Molecular functioni
- retinoid X receptor binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 202595, 12 interactors |
DIPi | DIP-42822N |
IntActi | P18911, 2 interactors |
MINTi | P18911 |
STRINGi | 10090.ENSMUSP00000048838 |
Chemistry databases
BindingDBi | P18911 |
Miscellaneous databases
RNActi | P18911, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 185 – 419 | NR LBDPROSITE-ProRule annotationAdd BLAST | 235 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 89 | ModulatingAdd BLAST | 89 | |
Regioni | 156 – 184 | HingeAdd BLAST | 29 |
Domaini
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 90 – 110 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 21 | |
Zinc fingeri | 126 – 150 | NR C4-typePROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000156458 |
HOGENOMi | CLU_007368_18_2_1 |
InParanoidi | P18911 |
OMAi | NKERLFP |
OrthoDBi | 1165737at2759 |
PhylomeDBi | P18911 |
TreeFami | TF328382 |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR003078, Retinoic_acid_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01292, RETNOICACIDR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: P18911-1) [UniParc]FASTAAdd to basket
Also known as: A
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MATNKERLFA PGALGPGSGY PGAGFPFAFP GALRGSPPFE MLSPSFRGLG
60 70 80 90 100
QPDLPKEMAS LSVETQSTSS EEMVPSSPSP PPPPRVYKPC FVCNDKSSGY
110 120 130 140 150
HYGVSSCEGC KGFFRRSIQK NMVYTCHRDK NCIINKVTRN RCQYCRLQKC
160 170 180 190 200
FEVGMSKEAV RNDRNKKKKE VKEEGSPDSY ELSPQLEELI TKVSKAHQET
210 220 230 240 250
FPSLCQLGKY TTNSSADHRV QLDLGLWDKF SELATKCIIK IVEFAKRLPG
260 270 280 290 300
FTGLSIADQI TLLKAACLDI LMLRICTRYT PEQDTMTFSD GLTLNRTQMH
310 320 330 340 350
NAGFGPLTDL VFAFAGQLLP LEMDDTETGL LSAICLICGD RMDLEEPEKV
360 370 380 390 400
DKLQEPLLEA LRLYARRRRP SQPYMFPRML MKITDLRGIS TKGAERAITL
410 420 430 440 450
KMEIPGPMPP LIREMLENPE MFEDDSSKPG PHPKASSEDE APGGQGKRGQ
SPQPDQGP
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3YZY4 | D3YZY4_MOUSE | Retinoic acid receptor gamma | Rarg | 227 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 | V → E in BAE28323 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 78 | P → A in BAE28323 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 176 | S → L in AAH13709 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 369 – 372 | RPSQ → DPAK in AAA40035 (PubMed:2157970).Curated | 4 | |
Sequence conflicti | 369 – 372 | RPSQ → DPAK in AAA40036 (PubMed:2157970).Curated | 4 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031081 | 1 – 62 | MATNK…MASLS → MHKGDNSGHQQVPRKRGHGM RVLLLSFCLSSHAAFHSA in isoform 3. 1 PublicationAdd BLAST | 62 | |
Alternative sequenceiVSP_031082 | 1 – 61 | MATNK…EMASL → MYDCMESFVPGPRRLYGAAG PGAGLLRRATGSSCFAGLES FAWAQPASLQ in isoform 2. 2 PublicationsAdd BLAST | 61 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15848 mRNA Translation: CAA33845.1 M34475 mRNA Translation: AAA40036.1 M34476 mRNA Translation: AAA40035.1 AK148064 mRNA Translation: BAE28323.1 BC012923 mRNA Translation: AAH12923.1 BC013709 mRNA Translation: AAH13709.1 M32068 mRNA Translation: AAA40032.1 M32069 mRNA Translation: AAA40033.1 M32070 mRNA Translation: AAA40034.1 |
CCDSi | CCDS27875.1 [P18911-1] CCDS37226.1 [P18911-2] |
PIRi | B34714 S05052, A34714 |
RefSeqi | NP_001036192.1, NM_001042727.2 [P18911-2] NP_035374.3, NM_011244.4 [P18911-1] XP_006520713.1, XM_006520650.1 [P18911-1] XP_011243827.1, XM_011245525.2 [P18911-1] |
Genome annotation databases
Ensembli | ENSMUST00000043172; ENSMUSP00000048838; ENSMUSG00000001288 [P18911-1] ENSMUST00000063339; ENSMUSP00000067266; ENSMUSG00000001288 [P18911-2] |
GeneIDi | 19411 |
KEGGi | mmu:19411 |
UCSCi | uc007xvc.2, mouse [P18911-2] uc007xvd.2, mouse [P18911-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15848 mRNA Translation: CAA33845.1 M34475 mRNA Translation: AAA40036.1 M34476 mRNA Translation: AAA40035.1 AK148064 mRNA Translation: BAE28323.1 BC012923 mRNA Translation: AAH12923.1 BC013709 mRNA Translation: AAH13709.1 M32068 mRNA Translation: AAA40032.1 M32069 mRNA Translation: AAA40033.1 M32070 mRNA Translation: AAA40034.1 |
CCDSi | CCDS27875.1 [P18911-1] CCDS37226.1 [P18911-2] |
PIRi | B34714 S05052, A34714 |
RefSeqi | NP_001036192.1, NM_001042727.2 [P18911-2] NP_035374.3, NM_011244.4 [P18911-1] XP_006520713.1, XM_006520650.1 [P18911-1] XP_011243827.1, XM_011245525.2 [P18911-1] |
3D structure databases
SMRi | P18911 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202595, 12 interactors |
DIPi | DIP-42822N |
IntActi | P18911, 2 interactors |
MINTi | P18911 |
STRINGi | 10090.ENSMUSP00000048838 |
Chemistry databases
BindingDBi | P18911 |
ChEMBLi | CHEMBL4177 |
DrugCentrali | P18911 |
GuidetoPHARMACOLOGYi | 592 |
PTM databases
iPTMneti | P18911 |
PhosphoSitePlusi | P18911 |
Proteomic databases
MaxQBi | P18911 |
PaxDbi | P18911 |
PeptideAtlasi | P18911 |
PRIDEi | P18911 |
Protocols and materials databases
Antibodypediai | 15142, 196 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000043172; ENSMUSP00000048838; ENSMUSG00000001288 [P18911-1] ENSMUST00000063339; ENSMUSP00000067266; ENSMUSG00000001288 [P18911-2] |
GeneIDi | 19411 |
KEGGi | mmu:19411 |
UCSCi | uc007xvc.2, mouse [P18911-2] uc007xvd.2, mouse [P18911-1] |
Organism-specific databases
CTDi | 5916 |
MGIi | MGI:97858, Rarg |
Phylogenomic databases
eggNOGi | KOG3575, Eukaryota |
GeneTreei | ENSGT00940000156458 |
HOGENOMi | CLU_007368_18_2_1 |
InParanoidi | P18911 |
OMAi | NKERLFP |
OrthoDBi | 1165737at2759 |
PhylomeDBi | P18911 |
TreeFami | TF328382 |
Enzyme and pathway databases
Reactomei | R-MMU-383280, Nuclear Receptor transcription pathway R-MMU-5362517, Signaling by Retinoic Acid |
Miscellaneous databases
BioGRID-ORCSi | 19411, 1 hit in 17 CRISPR screens |
ChiTaRSi | Rarg, mouse |
PROi | PR:P18911 |
RNActi | P18911, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000001288, Expressed in lip and 348 other tissues |
ExpressionAtlasi | P18911, baseline and differential |
Genevisiblei | P18911, MM |
Family and domain databases
Gene3Di | 1.10.565.10, 1 hit 3.30.50.10, 1 hit |
InterProi | View protein in InterPro IPR035500, NHR-like_dom_sf IPR000536, Nucl_hrmn_rcpt_lig-bd IPR001723, Nuclear_hrmn_rcpt IPR003078, Retinoic_acid_rcpt IPR001628, Znf_hrmn_rcpt IPR013088, Znf_NHR/GATA |
Pfami | View protein in Pfam PF00104, Hormone_recep, 1 hit PF00105, zf-C4, 1 hit |
PRINTSi | PR01292, RETNOICACIDR PR00398, STRDHORMONER PR00047, STROIDFINGER |
SMARTi | View protein in SMART SM00430, HOLI, 1 hit SM00399, ZnF_C4, 1 hit |
SUPFAMi | SSF48508, SSF48508, 1 hit |
PROSITEi | View protein in PROSITE PS51843, NR_LBD, 1 hit PS00031, NUCLEAR_REC_DBD_1, 1 hit PS51030, NUCLEAR_REC_DBD_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RARG_MOUSE | |
Accessioni | P18911Primary (citable) accession number: P18911 Secondary accession number(s): P20787 Q91YX2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | February 5, 2008 | |
Last modified: | February 10, 2021 | |
This is version 204 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families