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Protein

Atrial natriuretic peptide receptor 1

Gene

Npr1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand (By similarity).By similarity

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81Chloride2 Publications1
Binding sitei113Chloride; via amide nitrogen2 Publications1
Binding sitei114Chloride; via amide nitrogen2 Publications1

GO - Molecular functioni

  • ATP binding Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: RGD
  • natriuretic peptide receptor activity Source: Ensembl
  • peptide hormone binding Source: Ensembl
  • protein kinase activity Source: InterPro
  • protein kinase binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionLyase, Receptor, Vasoactive
Biological processcGMP biosynthesis
LigandChloride, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi4.6.1.2 5301
ReactomeiR-RNO-5578768 Physiological factors

Names & Taxonomyi

Protein namesi
Recommended name:
Atrial natriuretic peptide receptor 1 (EC:4.6.1.2)
Alternative name(s):
Atrial natriuretic peptide receptor type A
Short name:
ANP-A
Short name:
ANPR-A
Short name:
NPR-A
Guanylate cyclase A
Short name:
GC-A
Gene namesi
Name:Npr1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi3195 Npr1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 469ExtracellularSequence analysisAdd BLAST441
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 1057CytoplasmicSequence analysisAdd BLAST567

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi197E → K: Abolishes hormone binding. 1 Publication1
Mutagenesisi213H → D: Abolishes hormone binding. 1 Publication1
Mutagenesisi522S → A: No effect on phosphorylation, 15% loss of ANP-dependent activity. 1 Publication1
Mutagenesisi525S → A: Reduced phosphorylation, 80% loss of ANP-dependent activity. 1 Publication1
Mutagenesisi525S → E: No effect on ANP-dependent activity. 1 Publication1
Mutagenesisi528T → A: Reduced phosphorylation, 50% loss of ANP-dependent activity. 1 Publication1
Mutagenesisi530S → A: Reduced phosphorylation, 50% loss of ANP-dependent activity. 1 Publication1
Mutagenesisi534S → A: Reduced phosphorylation, 50% loss of ANP-dependent activity. 1 Publication1
Mutagenesisi538S → A: Reduced phosphorylation, 60% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi541T → A: Markedly reduced phosphorylation, 50% loss of CNP-dependent activity. 1 Publication1
Mutagenesisi542T → A: No effect on phosphorylation, 30% loss of ANP-dependent activity. 1 Publication1

Chemistry databases

GuidetoPHARMACOLOGYi1747

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Add BLAST28
ChainiPRO_000001236229 – 1057Atrial natriuretic peptide receptor 1Add BLAST1029

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi41N-linked (GlcNAc...) asparagine1
Disulfide bondi88 ↔ 1141 Publication
Disulfide bondi192 ↔ 2411 Publication
Glycosylationi208N-linked (GlcNAc...) asparagine2 Publications1
Glycosylationi334N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi375N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi382N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi423N-linked (GlcNAc...) asparagine1
Disulfide bondi451 ↔ 4601 Publication
Modified residuei515Phosphoserine1 Publication1
Modified residuei525Phosphoserine2 Publications1
Modified residuei528Phosphothreonine2 Publications1
Modified residuei530Phosphoserine2 Publications1
Modified residuei534Phosphoserine2 Publications1
Modified residuei538Phosphoserine2 Publications1
Modified residuei541Phosphothreonine2 Publications1

Post-translational modificationi

Phosphorylation of the protein kinase-like domain is required for full activation by ANP.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP18910
PRIDEiP18910

PTM databases

iPTMnetiP18910
PhosphoSitePlusiP18910

Expressioni

Gene expression databases

BgeeiENSRNOG00000014684 Expressed in 10 organ(s), highest expression level in lung
GenevisibleiP18910 RN

Interactioni

Subunit structurei

Homodimer.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

ComplexPortaliCPX-33 ANPR-A receptor complex
IntActiP18910, 2 interactors
MINTiP18910
STRINGi10116.ENSRNOP00000020307

Structurei

Secondary structure

11057
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP18910
SMRiP18910
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP18910

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini524 – 801Protein kinasePROSITE-ProRule annotationAdd BLAST278
Domaini872 – 1002Guanylate cyclasePROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1023 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000293307
HOVERGENiHBG051862
InParanoidiP18910
KOiK12323
OMAiREDPSML
OrthoDBiEOG091G02QS
PhylomeDBiP18910
TreeFamiTF106338

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR001828 ANF_lig-bd_rcpt
IPR001170 ANPR/GUC
IPR011009 Kinase-like_dom_sf
IPR029787 Nucleotide_cyclase
IPR028082 Peripla_BP_I
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF01094 ANF_receptor, 1 hit
PF00211 Guanylate_cyc, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00255 NATPEPTIDER
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit
SSF55073 SSF55073, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00458 ANF_RECEPTORS, 1 hit
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P18910-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPGSRRVRPR LRALLLLPPL LLLRGGHASD LTVAVVLPLT NTSYPWSWAR
60 70 80 90 100
VGPAVELALA RVKARPDLLP GWTVRMVLGS SENAAGVCSD TAAPLAAVDL
110 120 130 140 150
KWEHSPAVFL GPGCVYSAAP VGRFTAHWRV PLLTAGAPAL GIGVKDEYAL
160 170 180 190 200
TTRTGPSHVK LGDFVTALHR RLGWEHQALV LYADRLGDDR PCFFIVEGLY
210 220 230 240 250
MRVRERLNIT VNHQEFVEGD PDHYPKLLRA VRRKGRVIYI CSSPDAFRNL
260 270 280 290 300
MLLALNAGLT GEDYVFFHLD VFGQSLKSAQ GLVPQKPWER GDGQDRSARQ
310 320 330 340 350
AFQAAKIITY KEPDNPEYLE FLKQLKLLAD KKFNFTVEDG LKNIIPASFH
360 370 380 390 400
DGLLLYVQAV TETLAQGGTV TDGENITQRM WNRSFQGVTG YLKIDRNGDR
410 420 430 440 450
DTDFSLWDMD PETGAFRVVL NYNGTSQELM AVSEHKLYWP LGYPPPDVPK
460 470 480 490 500
CGFDNEDPAC NQDHFSTLEV LALVGSLSLI SFLIVSFFIY RKMQLEKELV
510 520 530 540 550
SELWRVRWED LQPSSLERHL RSAGSRLTLS GRGSNYGSLL TTEGQFQVFA
560 570 580 590 600
KTAYYKGNLV AVKRVNRKRI ELTRKVLFEL KHMRDVQNEH LTRFVGACTD
610 620 630 640 650
PPNICILTEY CPRGSLQDIL ENESITLDWM FRYSLTNDIV KGMLFLHNGA
660 670 680 690 700
ICSHGNLKSS NCVVDGRFVL KITDYGLESF RDPEPEQGHT LFAKKLWTAP
710 720 730 740 750
ELLRMASPPA RGSQAGDVYS FGIILQEIAL RSGVFYVEGL DLSPKEIIER
760 770 780 790 800
VTRGEQPPFR PSMDLQSHLE ELGQLMQRCW AEDPQERPPF QQIRLALRKF
810 820 830 840 850
NKENSSNILD NLLSRMEQYA NNLEELVEER TQAYLEEKRK AEALLYQILP
860 870 880 890 900
HSVAEQLKRG ETVQAEAFDS VTIYFSDIVG FTALSAESTP MQVVTLLNDL
910 920 930 940 950
YTCFDAVIDN FDVYKVETIG DAYMVVSGLP VRNGQLHARE VARMALALLD
960 970 980 990 1000
AVRSFRIRHR PQEQLRLRIG IHTGPVCAGV VGLKMPRYCL FGDTVNTASR
1010 1020 1030 1040 1050
MESNGEALKI HLSSETKAVL EEFDGFELEL RGDVEMKGKG KVRTYWLLGE

RGCSTRG
Length:1,057
Mass (Da):118,951
Last modified:November 1, 1990 - v1
Checksum:i9EA9AE685AC05816
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti366Q → H in AAA41202 (PubMed:1679239).Curated1
Sequence conflicti392L → P in AAA41202 (PubMed:1679239).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14773 mRNA Translation: CAA32881.1
J05677 Genomic DNA Translation: AAA41200.1
M74535 mRNA Translation: AAA41202.1
BC128742 mRNA Translation: AAI28743.1
PIRiS03348 OYRTR
RefSeqiNP_036745.1, NM_012613.1
XP_006232653.1, XM_006232591.3
UniGeneiRn.10463

Genome annotation databases

EnsembliENSRNOT00000020307; ENSRNOP00000020307; ENSRNOG00000014684
GeneIDi24603
KEGGirno:24603
UCSCiRGD:3195 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14773 mRNA Translation: CAA32881.1
J05677 Genomic DNA Translation: AAA41200.1
M74535 mRNA Translation: AAA41202.1
BC128742 mRNA Translation: AAI28743.1
PIRiS03348 OYRTR
RefSeqiNP_036745.1, NM_012613.1
XP_006232653.1, XM_006232591.3
UniGeneiRn.10463

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DP4X-ray2.00A/C29-463[»]
1T34X-ray2.95A/B29-463[»]
3A3KX-ray2.50A/B29-463[»]
ProteinModelPortaliP18910
SMRiP18910
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-33 ANPR-A receptor complex
IntActiP18910, 2 interactors
MINTiP18910
STRINGi10116.ENSRNOP00000020307

Chemistry databases

GuidetoPHARMACOLOGYi1747

PTM databases

iPTMnetiP18910
PhosphoSitePlusiP18910

Proteomic databases

PaxDbiP18910
PRIDEiP18910

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020307; ENSRNOP00000020307; ENSRNOG00000014684
GeneIDi24603
KEGGirno:24603
UCSCiRGD:3195 rat

Organism-specific databases

CTDi4881
RGDi3195 Npr1

Phylogenomic databases

eggNOGiKOG1023 Eukaryota
COG2114 LUCA
GeneTreeiENSGT00760000118959
HOGENOMiHOG000293307
HOVERGENiHBG051862
InParanoidiP18910
KOiK12323
OMAiREDPSML
OrthoDBiEOG091G02QS
PhylomeDBiP18910
TreeFamiTF106338

Enzyme and pathway databases

BRENDAi4.6.1.2 5301
ReactomeiR-RNO-5578768 Physiological factors

Miscellaneous databases

EvolutionaryTraceiP18910
PROiPR:P18910

Gene expression databases

BgeeiENSRNOG00000014684 Expressed in 10 organ(s), highest expression level in lung
GenevisibleiP18910 RN

Family and domain databases

Gene3Di3.30.70.1230, 1 hit
InterProiView protein in InterPro
IPR001054 A/G_cyclase
IPR018297 A/G_cyclase_CS
IPR001828 ANF_lig-bd_rcpt
IPR001170 ANPR/GUC
IPR011009 Kinase-like_dom_sf
IPR029787 Nucleotide_cyclase
IPR028082 Peripla_BP_I
IPR000719 Prot_kinase_dom
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
PfamiView protein in Pfam
PF01094 ANF_receptor, 1 hit
PF00211 Guanylate_cyc, 1 hit
PF07714 Pkinase_Tyr, 1 hit
PRINTSiPR00255 NATPEPTIDER
SMARTiView protein in SMART
SM00044 CYCc, 1 hit
SUPFAMiSSF53822 SSF53822, 1 hit
SSF55073 SSF55073, 1 hit
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00458 ANF_RECEPTORS, 1 hit
PS00452 GUANYLATE_CYCLASE_1, 1 hit
PS50125 GUANYLATE_CYCLASE_2, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiANPRA_RAT
AccessioniPrimary (citable) accession number: P18910
Secondary accession number(s): A1A5N8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1990
Last modified: October 10, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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