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Protein

Cyclic AMP-dependent transcription factor ATF-1

Gene

ATF1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-199920 CREB phosphorylation
SignaLinkiP18846
SIGNORiP18846

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclic AMP-dependent transcription factor ATF-1
Short name:
cAMP-dependent transcription factor ATF-1
Alternative name(s):
Activating transcription factor 1
Protein TREB36
Gene namesi
Name:ATF1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000123268.8
HGNCiHGNC:783 ATF1
MIMi123803 gene
neXtProtiNX_P18846

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Angiomatoid fibrous histiocytoma (AFH)
The gene represented in this entry may be involved in disease pathogenesis. Chromosomal aberrations involving ATF1 are found in patients with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with FUS generates a chimeric ATF1/FUS protein. Translocation t(12;22)(q13;q12) with EWSR1 generates a chimeric ATF1/EWSR1 protein.
Disease descriptionA distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis.
See also OMIM:612160

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63S → A: Impaired CDK3-mediated phosphorylation and altered transactivation and transcriptional activities. 1 Publication1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110Breakpoint for translocation to form chimeric EWSR1/ATF1 protein1
Sitei112Breakpoint for translocation to form chimeric FUS/ATF1 protein1

Organism-specific databases

DisGeNETi466
MalaCardsiATF1
MIMi612160 phenotype
OpenTargetsiENSG00000123268
Orphaneti97338 Melanoma of soft parts
PharmGKBiPA25083

Chemistry databases

ChEMBLiCHEMBL3255
DrugBankiDB00852 Pseudoephedrine

Polymorphism and mutation databases

BioMutaiATF1
DMDMi1168542

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765751 – 271Cyclic AMP-dependent transcription factor ATF-1Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63Phosphoserine; by CaMK1, CDK3, RPS6KA4 and RPS6KA52 Publications1
Modified residuei198Phosphoserine; by HIPK2PROSITE-ProRule annotationCombined sources1 Publication1
Cross-linki208Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki215Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Post-translational modificationi

Phosphorylated at Ser-198 by HIPK2 in response to genotoxic stress. This phosphorylation promotes transcription repression of FTH1 and other antioxidant detoxification genes. The CDK3-mediated phosphorylation at Ser-63 promotes its transactivation and transcriptional activities. Phosphorylated at Ser-63 by RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli.3 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP18846
MaxQBiP18846
PaxDbiP18846
PeptideAtlasiP18846
PRIDEiP18846
ProteomicsDBi53610

PTM databases

iPTMnetiP18846
PhosphoSitePlusiP18846

Expressioni

Gene expression databases

BgeeiENSG00000123268 Expressed in 230 organ(s), highest expression level in biceps brachii
CleanExiHS_ATF1
ExpressionAtlasiP18846 baseline and differential
GenevisibleiP18846 HS

Organism-specific databases

HPAiCAB016222
HPA055069
HPA055406

Interactioni

Subunit structurei

Binds DNA as a dimer. Interacts with HIPK2 and CDK3.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi106956, 32 interactors
ComplexPortaliCPX-9 ATF1-ATF4 transcription factor complex
DIPiDIP-652N
IntActiP18846, 12 interactors
MINTiP18846
STRINGi9606.ENSP00000262053

Chemistry databases

BindingDBiP18846

Structurei

3D structure databases

ProteinModelPortaliP18846
SMRiP18846
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 90KIDPROSITE-ProRule annotationAdd BLAST60
Domaini213 – 271bZIPPROSITE-ProRule annotationAdd BLAST59

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 239Basic motifPROSITE-ProRule annotationAdd BLAST25
Regioni241 – 262Leucine-zipperPROSITE-ProRule annotationAdd BLAST22

Sequence similaritiesi

Belongs to the bZIP family. ATF subfamily.Curated

Phylogenomic databases

eggNOGiKOG3584 Eukaryota
ENOG410ZZJZ LUCA
GeneTreeiENSGT00390000008655
HOGENOMiHOG000007365
HOVERGENiHBG011077
InParanoidiP18846
KOiK09053
OMAiHIAQQMS
OrthoDBiEOG091G0FTJ
PhylomeDBiP18846
TreeFamiTF106464

Family and domain databases

InterProiView protein in InterPro
IPR029825 ATF1
IPR004827 bZIP
IPR003102 Coactivator_CBP_pKID
IPR001630 Leuzip_CREB
PANTHERiPTHR22952:SF258 PTHR22952:SF258, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
PF02173 pKID, 1 hit
PRINTSiPR00041 LEUZIPPRCREB
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
PS50953 KID, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P18846-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDSHKSTTS ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS
60 70 80 90 100
SQKAHGILAR RPSYRKILKD LSSEDTRGRK GDGENSGVSA AVTSMSVPTP
110 120 130 140 150
IYQTSSGQYI AIAPNGALQL ASPGTDGVQG LQTLTMTNSG STQQGTTILQ
160 170 180 190 200
YAQTSDGQQI LVPSNQVVVQ TASGDMQTYQ IRTTPSATSL PQTVVMTSPV
210 220 230 240 250
TLTSQTTKTD DPQLKREIRL MKNREAAREC RRKKKEYVKC LENRVAVLEN
260 270
QNKTLIEELK TLKDLYSNKS V
Length:271
Mass (Da):29,233
Last modified:November 1, 1995 - v2
Checksum:iBAFECBFFB244FCED
GO
Isoform 2 (identifier: P18846-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: Missing.

Note: No experimental confirmation available.
Show »
Length:136
Mass (Da):15,295
Checksum:i86343F1CF592889F
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8VYN3F8VYN3_HUMAN
Cyclic AMP-dependent transcription ...
ATF1
200Annotation score:
F8VS03F8VS03_HUMAN
Cyclic AMP-dependent transcription ...
ATF1
170Annotation score:
F8VYE5F8VYE5_HUMAN
Cyclic AMP-dependent transcription ...
ATF1
55Annotation score:
F8VRN2F8VRN2_HUMAN
Cyclic AMP-dependent transcription ...
ATF1
77Annotation score:

Sequence cautioni

The sequence CAC15058 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55H → A no nucleotide entry (PubMed:2516827).Curated1
Sequence conflicti227Missing no nucleotide entry (PubMed:2516827).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024382191P → A. Corresponds to variant dbSNP:rs2230674Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0555591 – 135Missing in isoform 2. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55544 Genomic DNA Translation: CAA39150.1
AK299240 mRNA Translation: BAG61271.1
AC013244 Genomic DNA No translation available.
BC029619 mRNA Translation: AAH29619.1
AJ295163 mRNA Translation: CAC15058.1 Different initiation.
CCDSiCCDS8803.1 [P18846-1]
PIRiS12560
RefSeqiNP_005162.1, NM_005171.4 [P18846-1]
XP_011536688.1, XM_011538386.2 [P18846-1]
XP_011536689.1, XM_011538387.2 [P18846-1]
XP_016874821.1, XM_017019332.1 [P18846-1]
XP_016874822.1, XM_017019333.1 [P18846-1]
XP_016874823.1, XM_017019334.1 [P18846-1]
UniGeneiHs.648565

Genome annotation databases

EnsembliENST00000262053; ENSP00000262053; ENSG00000123268 [P18846-1]
GeneIDi466
KEGGihsa:466
UCSCiuc001rww.5 human [P18846-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55544 Genomic DNA Translation: CAA39150.1
AK299240 mRNA Translation: BAG61271.1
AC013244 Genomic DNA No translation available.
BC029619 mRNA Translation: AAH29619.1
AJ295163 mRNA Translation: CAC15058.1 Different initiation.
CCDSiCCDS8803.1 [P18846-1]
PIRiS12560
RefSeqiNP_005162.1, NM_005171.4 [P18846-1]
XP_011536688.1, XM_011538386.2 [P18846-1]
XP_011536689.1, XM_011538387.2 [P18846-1]
XP_016874821.1, XM_017019332.1 [P18846-1]
XP_016874822.1, XM_017019333.1 [P18846-1]
XP_016874823.1, XM_017019334.1 [P18846-1]
UniGeneiHs.648565

3D structure databases

ProteinModelPortaliP18846
SMRiP18846
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106956, 32 interactors
ComplexPortaliCPX-9 ATF1-ATF4 transcription factor complex
DIPiDIP-652N
IntActiP18846, 12 interactors
MINTiP18846
STRINGi9606.ENSP00000262053

Chemistry databases

BindingDBiP18846
ChEMBLiCHEMBL3255
DrugBankiDB00852 Pseudoephedrine

PTM databases

iPTMnetiP18846
PhosphoSitePlusiP18846

Polymorphism and mutation databases

BioMutaiATF1
DMDMi1168542

Proteomic databases

EPDiP18846
MaxQBiP18846
PaxDbiP18846
PeptideAtlasiP18846
PRIDEiP18846
ProteomicsDBi53610

Protocols and materials databases

DNASUi466
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262053; ENSP00000262053; ENSG00000123268 [P18846-1]
GeneIDi466
KEGGihsa:466
UCSCiuc001rww.5 human [P18846-1]

Organism-specific databases

CTDi466
DisGeNETi466
EuPathDBiHostDB:ENSG00000123268.8
GeneCardsiATF1
HGNCiHGNC:783 ATF1
HPAiCAB016222
HPA055069
HPA055406
MalaCardsiATF1
MIMi123803 gene
612160 phenotype
neXtProtiNX_P18846
OpenTargetsiENSG00000123268
Orphaneti97338 Melanoma of soft parts
PharmGKBiPA25083
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3584 Eukaryota
ENOG410ZZJZ LUCA
GeneTreeiENSGT00390000008655
HOGENOMiHOG000007365
HOVERGENiHBG011077
InParanoidiP18846
KOiK09053
OMAiHIAQQMS
OrthoDBiEOG091G0FTJ
PhylomeDBiP18846
TreeFamiTF106464

Enzyme and pathway databases

ReactomeiR-HSA-199920 CREB phosphorylation
SignaLinkiP18846
SIGNORiP18846

Miscellaneous databases

ChiTaRSiATF1 human
GeneWikiiATF1
GenomeRNAii466
PROiPR:P18846
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123268 Expressed in 230 organ(s), highest expression level in biceps brachii
CleanExiHS_ATF1
ExpressionAtlasiP18846 baseline and differential
GenevisibleiP18846 HS

Family and domain databases

InterProiView protein in InterPro
IPR029825 ATF1
IPR004827 bZIP
IPR003102 Coactivator_CBP_pKID
IPR001630 Leuzip_CREB
PANTHERiPTHR22952:SF258 PTHR22952:SF258, 1 hit
PfamiView protein in Pfam
PF00170 bZIP_1, 1 hit
PF02173 pKID, 1 hit
PRINTSiPR00041 LEUZIPPRCREB
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
PS00036 BZIP_BASIC, 1 hit
PS50953 KID, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATF1_HUMAN
AccessioniPrimary (citable) accession number: P18846
Secondary accession number(s): B4DRF9, P25168, Q9H4A8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
Last sequence update: November 1, 1995
Last modified: September 12, 2018
This is version 188 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
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Main funding by: National Institutes of Health

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