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UniProtKB - P18843 (NADE_ECOLI)
Protein
NH(3)-dependent NAD(+) synthetase
Gene
nadE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.
UniRule annotation1 PublicationCatalytic activityi
- EC:6.3.1.5UniRule annotation1 Publication
: NAD(+) biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | Deamido-NADCombined sources1 Publication | 1 | |
Metal bindingi | 52 | MagnesiumUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 82 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 88 | ATPCombined sources1 Publication | 1 | |
Binding sitei | 136 | Deamido-NADCombined sources1 Publication | 1 | |
Binding sitei | 140 | Deamido-NADUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 160 | ATPUniRule annotationCombined sources1 Publication | 1 | |
Metal bindingi | 165 | MagnesiumUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 173 | Deamido-NADUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 180 | Deamido-NADUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 189 | ATPUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 211 | ATP; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 46 – 53 | ATPUniRule annotationCombined sources1 Publication | 8 | |
Nucleotide bindingi | 260 – 261 | Deamido-NADUniRule annotationCombined sources1 Publication | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- glutaminase activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- NAD+ synthase (glutamine-hydrolyzing) activity Source: InterPro
- NAD+ synthase activity Source: EcoCyc
GO - Biological processi
- 'de novo' NAD biosynthetic process from aspartate Source: EcoCyc
- cellular response to DNA damage stimulus Source: EcoliWiki
- NAD biosynthetic process Source: GO_Central
- NAD salvage Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Magnesium, Metal-binding, NAD, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:NAD-SYNTH-MONOMER |
UniPathwayi | UPA00253;UER00333 |
Names & Taxonomyi
Protein namesi | Recommended name: NH(3)-dependent NAD(+) synthetase1 PublicationUniRule annotation (EC:6.3.1.5UniRule annotation1 Publication)Alternative name(s): Nicotinamide adenine dinucleotide synthetase Short name: NADS Nitrogen regulatory protein |
Gene namesi | Name:nadE1 PublicationUniRule annotation Synonyms:efg1 Publication, ntrL Ordered Locus Names:b1740, JW1729 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Chemistry databases
DrugBanki | DB04099, Deamido-Nad DB02937, Gamma-Arsono-Beta, Gamma-Methyleneadenosine-5'-Diphosphate DB04160, Pyrophosphoric acid |
DrugCentrali | P18843 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000152167 | 1 – 275 | NH(3)-dependent NAD(+) synthetaseAdd BLAST | 275 |
Post-translational modificationi
May be phosphorylated.
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P18843 |
PaxDbi | P18843 |
PRIDEi | P18843 |
2D gel databases
SWISS-2DPAGEi | P18843 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation2 PublicationsBinary interactionsi
P18843
With | #Exp. | IntAct |
---|---|---|
dnaA [P03004] | 3 | EBI-548960,EBI-548951 |
Protein-protein interaction databases
BioGRIDi | 4262229, 32 interactors 851285, 4 interactors |
DIPi | DIP-10295N |
IntActi | P18843, 479 interactors |
STRINGi | 511145.b1740 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P18843 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P18843 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0171, Bacteria |
HOGENOMi | CLU_059327_3_0_6 |
InParanoidi | P18843 |
PhylomeDBi | P18843 |
Family and domain databases
CDDi | cd00553, NAD_synthase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00193, NadE_ammonia_dep, 1 hit |
InterProi | View protein in InterPro IPR022310, NAD/GMP_synthase IPR003694, NAD_synthase IPR022926, NH(3)-dep_NAD(+)_synth IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR23090, PTHR23090, 1 hit |
Pfami | View protein in Pfam PF02540, NAD_synthase, 1 hit |
TIGRFAMsi | TIGR00552, nadE, 1 hit |
i Sequence
Sequence statusi: Complete.
P18843-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLQQQIIKA LGAKPQINAE EEIRRSVDFL KSYLQTYPFI KSLVLGISGG
60 70 80 90 100
QDSTLAGKLC QMAINELRLE TGNESLQFIA VRLPYGVQAD EQDCQDAIAF
110 120 130 140 150
IQPDRVLTVN IKGAVLASEQ ALREAGIELS DFVRGNEKAR ERMKAQYSIA
160 170 180 190 200
GMTSGVVVGT DHAAEAITGF FTKYGDGGTD INPLYRLNKR QGKQLLAALA
210 220 230 240 250
CPEHLYKKAP TADLEDDRPS LPDEVALGVT YDNIDDYLEG KNVPQQVART
260 270
IENWYLKTEH KRRPPITVFD DFWKK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 – 31 | AKPQI…VDFLK → ENRRLMLKRKFVVVSISE in AAA79852 (PubMed:3025172).CuratedAdd BLAST | 19 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15328 Genomic DNA Translation: AAA79852.1 U00096 Genomic DNA Translation: AAC74810.1 AP009048 Genomic DNA Translation: BAA15529.1 |
PIRi | D64933 |
RefSeqi | NP_416254.1, NC_000913.3 WP_000175026.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74810; AAC74810; b1740 BAA15529; BAA15529; BAA15529 |
GeneIDi | 946946 |
KEGGi | ecj:JW1729 eco:b1740 |
PATRICi | fig|1411691.4.peg.516 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M15328 Genomic DNA Translation: AAA79852.1 U00096 Genomic DNA Translation: AAC74810.1 AP009048 Genomic DNA Translation: BAA15529.1 |
PIRi | D64933 |
RefSeqi | NP_416254.1, NC_000913.3 WP_000175026.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WXE | X-ray | 1.90 | A | 1-275 | [»] | |
1WXF | X-ray | 2.30 | A | 1-275 | [»] | |
1WXG | X-ray | 1.90 | A | 1-275 | [»] | |
1WXH | X-ray | 1.90 | A | 1-275 | [»] | |
1WXI | X-ray | 1.70 | A | 1-275 | [»] | |
SMRi | P18843 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262229, 32 interactors 851285, 4 interactors |
DIPi | DIP-10295N |
IntActi | P18843, 479 interactors |
STRINGi | 511145.b1740 |
Chemistry databases
DrugBanki | DB04099, Deamido-Nad DB02937, Gamma-Arsono-Beta, Gamma-Methyleneadenosine-5'-Diphosphate DB04160, Pyrophosphoric acid |
DrugCentrali | P18843 |
2D gel databases
SWISS-2DPAGEi | P18843 |
Proteomic databases
jPOSTi | P18843 |
PaxDbi | P18843 |
PRIDEi | P18843 |
Genome annotation databases
EnsemblBacteriai | AAC74810; AAC74810; b1740 BAA15529; BAA15529; BAA15529 |
GeneIDi | 946946 |
KEGGi | ecj:JW1729 eco:b1740 |
PATRICi | fig|1411691.4.peg.516 |
Organism-specific databases
EchoBASEi | EB0657 |
Phylogenomic databases
eggNOGi | COG0171, Bacteria |
HOGENOMi | CLU_059327_3_0_6 |
InParanoidi | P18843 |
PhylomeDBi | P18843 |
Enzyme and pathway databases
UniPathwayi | UPA00253;UER00333 |
BioCyci | EcoCyc:NAD-SYNTH-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P18843 |
PROi | PR:P18843 |
Family and domain databases
CDDi | cd00553, NAD_synthase, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00193, NadE_ammonia_dep, 1 hit |
InterProi | View protein in InterPro IPR022310, NAD/GMP_synthase IPR003694, NAD_synthase IPR022926, NH(3)-dep_NAD(+)_synth IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR23090, PTHR23090, 1 hit |
Pfami | View protein in Pfam PF02540, NAD_synthase, 1 hit |
TIGRFAMsi | TIGR00552, nadE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NADE_ECOLI | |
Accessioni | P18843Primary (citable) accession number: P18843 Secondary accession number(s): P78235 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | November 1, 1997 | |
Last modified: | February 23, 2022 | |
This is version 184 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families