UniProtKB - P18775 (DMSA_ECOLI)
Protein
Dimethyl sulfoxide reductase DmsA
Gene
dmsA
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the reduction of dimethyl sulfoxide (DMSO) to dimethyl sulfide (DMS). DMSO reductase serves as the terminal reductase under anaerobic conditions, with DMSO being the terminal electron acceptor. Terminal reductase during anaerobic growth on various sulfoxides and N-oxide compounds. Allows E.coli to grow anaerobically on DMSO as respiratory oxidant.1 Publication
Miscellaneous
The Tat signal sequence is essential for the expression of dmsA, the stability of the DmsAB dimer and membrane targeting. Despite the presence of a signal sequence, DmsA is not exported to the periplasm.
Catalytic activityi
- EC:1.8.5.3
Cofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
- Mo-bis(molybdopterin guanine dinucleotide)3 PublicationsNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.3 Publications
Activity regulationi
Inhibited by dithionite, sodium hydrogensulfite and tungstate.2 Publications
Kineticsi
- KM=0.043 mM for 2-chloropyridine N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
- KM=0.045 mM for 3-amidopyridine N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
- KM=0.06 mM for tertramethylene sulfoxide (at pH 5 and at 30 degrees Celsius)1 Publication
- KM=0.09 mM for methionine sulfoxide (at pH 5 and at 30 degrees Celsius)1 Publication
- KM=0.246 mM for 4-phenylpyridine N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
- KM=0.830 mM for dimethyldodecylamin N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
- KM=0.18 mM for DMSO (at pH 6.8 and at 23 degrees Celsius)1 Publication
- KM=0.47 mM for L-methionine sulfoxide (at pH 6.8 and at 23 degrees Celsius)1 Publication
- KM=0.5 mM for nicotinamide N-oxide (at pH 6.8 and at 23 degrees Celsius)1 Publication
- KM=0.6 mM for TMAO (at pH 6.8 and at 23 degrees Celsius)1 Publication
- KM=1.0 mM for 4-picoline N-oxide (at pH 6.8 and at 23 degrees Celsius)1 Publication
- KM=20.2 mM for TMAO (at pH 5 and at 30 degrees Celsius)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 63 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 67 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 71 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 104 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 205 | MolybdenumBy similarity | 1 | |
Binding sitei | 390 | Molybdopterin guanine dinucleotide 1By similarity | 1 | |
Binding sitei | 488 | Molybdopterin guanine dinucleotide 1By similarity | 1 | |
Binding sitei | 788 | Molybdopterin guanine dinucleotide 1By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- dimethyl sulfoxide reductase activity Source: InterPro
- electron transfer activity Source: EcoCyc
- molybdenum ion binding Source: GO_Central
- molybdopterin cofactor binding Source: InterPro
GO - Biological processi
- anaerobic respiration Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum |
Enzyme and pathway databases
BioCyci | EcoCyc:DMSA-MONOMER MetaCyc:DMSA-MONOMER |
BRENDAi | 1.8.5.3, 2026 |
Protein family/group databases
TCDBi | 5.A.3.3.2, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Names & Taxonomyi
Protein namesi | Recommended name: Dimethyl sulfoxide reductase DmsA (EC:1.8.5.3)Short name: DMSO reductase Short name: DMSOR Short name: Me2SO reductase |
Gene namesi | Name:dmsA Ordered Locus Names:b0894, JW5118 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Peripheral membrane protein 2 Publications; Cytoplasmic side 2 Publications
Plasma Membrane
- intrinsic component of periplasmic side of plasma membrane Source: EcoCyc
Other locations
- dimethyl sulfoxide reductase complex Source: EcoCyc
- outer membrane-bounded periplasmic space Source: GO_Central
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 17 | R → S: Not targeted to the membrane, does not support anaerobic growth. 1 Publication | 1 | |
Mutagenesisi | 57 | K → D: No alteration of the growth, expression, or catalytic activities. 1 Publication | 1 | |
Mutagenesisi | 67 | C → S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry. 2 Publications | 1 | |
Mutagenesisi | 71 | C → S: Cannot support growth. 1 Publication | 1 | |
Mutagenesisi | 104 | C → S: No alteration of the growth, expression, or catalytic activities. 1 Publication | 1 | |
Mutagenesisi | 106 | R → S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 45 | Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST | 45 | |
ChainiPRO_0000019143 | 46 – 814 | Dimethyl sulfoxide reductase DmsAAdd BLAST | 769 |
Post-translational modificationi
Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. Can also be exported by the Sec system.
Proteomic databases
jPOSTi | P18775 |
PaxDbi | P18775 |
PRIDEi | P18775 |
Interactioni
Subunit structurei
Heterotrimeric enzyme composed of a catalytic heterodimer (DmsAB) and a membrane anchor protein (DmsC).
1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4261945, 69 interactors 849882, 3 interactors |
ComplexPortali | CPX-320, DmaABC DMSO reductase complex |
DIPi | DIP-9452N |
IntActi | P18775, 8 interactors |
MINTi | P18775 |
STRINGi | 511145.b0894 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 56 – 118 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 63 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 172 – 176 | MGD 1 bindingBy similarity | 5 | |
Regioni | 244 – 245 | MGD 2 bindingBy similarity | 2 | |
Regioni | 270 – 271 | MGD 2 bindingBy similarity | 2 | |
Regioni | 291 – 293 | MGD 2 bindingBy similarity | 3 | |
Regioni | 386 – 387 | MGD 2 bindingBy similarity | 2 | |
Regioni | 512 – 513 | MGD 1 bindingBy similarity | 2 | |
Regioni | 701 | MGD 1 bindingBy similarity | 1 | |
Regioni | 707 – 709 | MGD 1 bindingBy similarity | 3 | |
Regioni | 804 – 805 | MGD 1 bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0243, Bacteria |
HOGENOMi | CLU_000422_13_3_6 |
InParanoidi | P18775 |
PhylomeDBi | P18775 |
Family and domain databases
InterProi | View protein in InterPro IPR011888, Anaer_DMSO_reductase IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS IPR006311, TAT_signal IPR019546, TAT_signal_bac_arc |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR02166, dmsA_ynfE, 1 hit TIGR01409, TAT_signal_seq, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00490, MOLYBDOPTERIN_PROK_2, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P18775-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTKIPDAVL AAEVSRRGLV KTTAIGGLAM ASSALTLPFS RIAHAVDSAI
60 70 80 90 100
PTKSDEKVIW SACTVNCGSR CPLRMHVVDG EIKYVETDNT GDDNYDGLHQ
110 120 130 140 150
VRACLRGRSM RRRVYNPDRL KYPMKRVGAR GEGKFERISW EEAYDIIATN
160 170 180 190 200
MQRLIKEYGN ESIYLNYGTG TLGGTMTRSW PPGNTLVARL MNCCGGYLNH
210 220 230 240 250
YGDYSSAQIA EGLNYTYGGW ADGNSPSDIE NSKLVVLFGN NPGETRMSGG
260 270 280 290 300
GVTYYLEQAR QKSNARMIII DPRYTDTGAG REDEWIPIRP GTDAALVNGL
310 320 330 340 350
AYVMITENLV DQAFLDKYCV GYDEKTLPAS APKNGHYKAY ILGEGPDGVA
360 370 380 390 400
KTPEWASQIT GVPADKIIKL AREIGSTKPA FISQGWGPQR HANGEIATRA
410 420 430 440 450
ISMLAILTGN VGINGGNSGA REGSYSLPFV RMPTLENPIQ TSISMFMWTD
460 470 480 490 500
AIERGPEMTA LRDGVRGKDK LDVPIKMIWN YAGNCLINQH SEINRTHEIL
510 520 530 540 550
QDDKKCELIV VIDCHMTSSA KYADILLPDC TASEQMDFAL DASCGNMSYV
560 570 580 590 600
IFNDQVIKPR FECKTIYEMT SELAKRLGVE QQFTEGRTQE EWMRHLYAQS
610 620 630 640 650
REAIPELPTF EEFRKQGIFK KRDPQGHHVA YKAFREDPQA NPLTTPSGKI
660 670 680 690 700
EIYSQALADI AATWELPEGD VIDPLPIYTP GFESYQDPLN KQYPLQLTGF
710 720 730 740 750
HYKSRVHSTY GNVDVLKAAC RQEMWINPLD AQKRGIHNGD KVRIFNDRGE
760 770 780 790 800
VHIEAKVTPR MMPGVVALGE GAWYDPDAKR VDKGGCINVL TTQRPSPLAK
810
GNPSHTNLVQ VEKV
Sequence cautioni
The sequence AAA83843 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03412 Genomic DNA Translation: AAA83843.1 Different initiation. U00096 Genomic DNA Translation: AAC73980.2 AP009048 Genomic DNA Translation: BAA35626.2 |
PIRi | S03785 |
RefSeqi | NP_415414.4, NC_000913.3 WP_000850303.1, NZ_SSZK01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC73980; AAC73980; b0894 BAA35626; BAA35626; BAA35626 |
GeneIDi | 945508 |
KEGGi | ecj:JW5118 eco:b0894 |
PATRICi | fig|1411691.4.peg.1383 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J03412 Genomic DNA Translation: AAA83843.1 Different initiation. U00096 Genomic DNA Translation: AAC73980.2 AP009048 Genomic DNA Translation: BAA35626.2 |
PIRi | S03785 |
RefSeqi | NP_415414.4, NC_000913.3 WP_000850303.1, NZ_SSZK01000002.1 |
3D structure databases
SMRi | P18775 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261945, 69 interactors 849882, 3 interactors |
ComplexPortali | CPX-320, DmaABC DMSO reductase complex |
DIPi | DIP-9452N |
IntActi | P18775, 8 interactors |
MINTi | P18775 |
STRINGi | 511145.b0894 |
Protein family/group databases
TCDBi | 5.A.3.3.2, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Proteomic databases
jPOSTi | P18775 |
PaxDbi | P18775 |
PRIDEi | P18775 |
Genome annotation databases
EnsemblBacteriai | AAC73980; AAC73980; b0894 BAA35626; BAA35626; BAA35626 |
GeneIDi | 945508 |
KEGGi | ecj:JW5118 eco:b0894 |
PATRICi | fig|1411691.4.peg.1383 |
Organism-specific databases
EchoBASEi | EB0228 |
Phylogenomic databases
eggNOGi | COG0243, Bacteria |
HOGENOMi | CLU_000422_13_3_6 |
InParanoidi | P18775 |
PhylomeDBi | P18775 |
Enzyme and pathway databases
BioCyci | EcoCyc:DMSA-MONOMER MetaCyc:DMSA-MONOMER |
BRENDAi | 1.8.5.3, 2026 |
Miscellaneous databases
PROi | PR:P18775 |
Family and domain databases
InterProi | View protein in InterPro IPR011888, Anaer_DMSO_reductase IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS IPR006311, TAT_signal IPR019546, TAT_signal_bac_arc |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR02166, dmsA_ynfE, 1 hit TIGR01409, TAT_signal_seq, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00490, MOLYBDOPTERIN_PROK_2, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit PS51318, TAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DMSA_ECOLI | |
Accessioni | P18775Primary (citable) accession number: P18775 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 10, 2021 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families