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Protein

Dimethyl sulfoxide reductase DmsA

Gene

dmsA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of dimethyl sulfoxide (DMSO) to dimethyl sulfide (DMS). DMSO reductase serves as the terminal reductase under anaerobic conditions, with DMSO being the terminal electron acceptor. Terminal reductase during anaerobic growth on various sulfoxides and N-oxide compounds. Allows E.coli to grow anaerobically on DMSO as respiratory oxidant.1 Publication

Miscellaneous

The Tat signal sequence is essential for the expression of dmsA, the stability of the DmsAB dimer and membrane targeting. Despite the presence of a signal sequence, DmsA is not exported to the periplasm.

Catalytic activityi

Dimethylsulfide + menaquinone + H2O = dimethylsulfoxide + menaquinol.

Cofactori

Protein has several cofactor binding sites:

Activity regulationi

Inhibited by dithionite, sodium hydrogensulfite and tungstate.2 Publications

Kineticsi

  1. KM=0.043 mM for 2-chloropyridine N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
  2. KM=0.045 mM for 3-amidopyridine N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
  3. KM=0.06 mM for tertramethylene sulfoxide (at pH 5 and at 30 degrees Celsius)1 Publication
  4. KM=0.09 mM for methionine sulfoxide (at pH 5 and at 30 degrees Celsius)1 Publication
  5. KM=0.246 mM for 4-phenylpyridine N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
  6. KM=0.830 mM for dimethyldodecylamin N-oxide (at pH 5 and at 30 degrees Celsius)1 Publication
  7. KM=0.18 mM for DMSO (at pH 6.8 and at 23 degrees Celsius)1 Publication
  8. KM=0.47 mM for L-methionine sulfoxide (at pH 6.8 and at 23 degrees Celsius)1 Publication
  9. KM=0.5 mM for nicotinamide N-oxide (at pH 6.8 and at 23 degrees Celsius)1 Publication
  10. KM=0.6 mM for TMAO (at pH 6.8 and at 23 degrees Celsius)1 Publication
  11. KM=1.0 mM for 4-picoline N-oxide (at pH 6.8 and at 23 degrees Celsius)1 Publication
  12. KM=20.2 mM for TMAO (at pH 5 and at 30 degrees Celsius)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi63Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
    Metal bindingi67Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
    Metal bindingi71Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
    Metal bindingi104Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
    Metal bindingi205MolybdenumBy similarity1
    Binding sitei390Molybdopterin guanine dinucleotide 1By similarity1
    Binding sitei488Molybdopterin guanine dinucleotide 1By similarity1
    Binding sitei788Molybdopterin guanine dinucleotide 1By similarity1

    GO - Molecular functioni

    • 4 iron, 4 sulfur cluster binding Source: EcoCyc
    • cofactor binding Source: EcoCyc
    • dimethyl sulfoxide reductase activity Source: InterPro
    • electron transfer activity Source: EcoCyc
    • molybdenum ion binding Source: GO_Central
    • molybdopterin cofactor binding Source: InterPro

    GO - Biological processi

    • anaerobic respiration Source: EcoCyc

    Keywordsi

    Molecular functionOxidoreductase
    Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciEcoCyc:DMSA-MONOMER
    MetaCyc:DMSA-MONOMER
    BRENDAi1.8.5.3 2026

    Protein family/group databases

    TCDBi5.A.3.3.2 the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dimethyl sulfoxide reductase DmsA (EC:1.8.5.3)
    Short name:
    DMSO reductase
    Short name:
    DMSOR
    Short name:
    Me2SO reductase
    Gene namesi
    Name:dmsA
    Ordered Locus Names:b0894, JW5118
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10232 dmsA

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi17R → S: Not targeted to the membrane, does not support anaerobic growth. 1 Publication1
    Mutagenesisi57K → D: No alteration of the growth, expression, or catalytic activities. 1 Publication1
    Mutagenesisi67C → S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry. 2 Publications1
    Mutagenesisi71C → S: Cannot support growth. 1 Publication1
    Mutagenesisi104C → S: No alteration of the growth, expression, or catalytic activities. 1 Publication1
    Mutagenesisi106R → S: Electron transfer from the 4Fe-4S clusters of DmsB to the Mo-bisMGD of DmsA is blocked. Little effect on the coordination sphere of the molybdenum and only minor effects on its redox chemistry. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 45Tat-type signalPROSITE-ProRule annotation1 PublicationAdd BLAST45
    ChainiPRO_000001914346 – 814Dimethyl sulfoxide reductase DmsAAdd BLAST769

    Post-translational modificationi

    Exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven. Can also be exported by the Sec system.

    Proteomic databases

    PaxDbiP18775
    PRIDEiP18775

    Interactioni

    Subunit structurei

    Heterotrimeric enzyme composed of a catalytic heterodimer (DmsAB) and a membrane anchor protein (DmsC).1 Publication

    Binary interactionsi

    Protein-protein interaction databases

    BioGridi4261945, 69 interactors
    ComplexPortaliCPX-320 DMSO reductase complex
    DIPiDIP-9452N
    IntActiP18775, 8 interactors
    MINTiP18775
    STRINGi316385.ECDH10B_0964

    Structurei

    3D structure databases

    ProteinModelPortaliP18775
    SMRiP18775
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini56 – 1184Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST63

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni172 – 176MGD 1 bindingBy similarity5
    Regioni244 – 245MGD 2 bindingBy similarity2
    Regioni270 – 271MGD 2 bindingBy similarity2
    Regioni291 – 293MGD 2 bindingBy similarity3
    Regioni386 – 387MGD 2 bindingBy similarity2
    Regioni512 – 513MGD 1 bindingBy similarity2
    Regioni701MGD 1 bindingBy similarity1
    Regioni707 – 709MGD 1 bindingBy similarity3
    Regioni804 – 805MGD 1 bindingBy similarity2

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4107QY8 Bacteria
    COG0243 LUCA
    HOGENOMiHOG000284390
    InParanoidiP18775
    KOiK07306
    PhylomeDBiP18775

    Family and domain databases

    InterProiView protein in InterPro
    IPR011888 Anaer_DMSO_reductase
    IPR009010 Asp_de-COase-like_dom_sf
    IPR006657 MoPterin_dinucl-bd_dom
    IPR006656 Mopterin_OxRdtase
    IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
    IPR006655 Mopterin_OxRdtase_prok_CS
    IPR027467 MopterinOxRdtase_cofactor_BS
    IPR006311 TAT_signal
    IPR019546 TAT_signal_bac_arc
    PfamiView protein in Pfam
    PF04879 Molybdop_Fe4S4, 1 hit
    PF00384 Molybdopterin, 1 hit
    PF01568 Molydop_binding, 1 hit
    SMARTiView protein in SMART
    SM00926 Molybdop_Fe4S4, 1 hit
    SUPFAMiSSF50692 SSF50692, 1 hit
    TIGRFAMsiTIGR02166 dmsA_ynfE, 1 hit
    TIGR01409 TAT_signal_seq, 1 hit
    PROSITEiView protein in PROSITE
    PS51669 4FE4S_MOW_BIS_MGD, 1 hit
    PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
    PS00490 MOLYBDOPTERIN_PROK_2, 1 hit
    PS00932 MOLYBDOPTERIN_PROK_3, 1 hit
    PS51318 TAT, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P18775-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKTKIPDAVL AAEVSRRGLV KTTAIGGLAM ASSALTLPFS RIAHAVDSAI
    60 70 80 90 100
    PTKSDEKVIW SACTVNCGSR CPLRMHVVDG EIKYVETDNT GDDNYDGLHQ
    110 120 130 140 150
    VRACLRGRSM RRRVYNPDRL KYPMKRVGAR GEGKFERISW EEAYDIIATN
    160 170 180 190 200
    MQRLIKEYGN ESIYLNYGTG TLGGTMTRSW PPGNTLVARL MNCCGGYLNH
    210 220 230 240 250
    YGDYSSAQIA EGLNYTYGGW ADGNSPSDIE NSKLVVLFGN NPGETRMSGG
    260 270 280 290 300
    GVTYYLEQAR QKSNARMIII DPRYTDTGAG REDEWIPIRP GTDAALVNGL
    310 320 330 340 350
    AYVMITENLV DQAFLDKYCV GYDEKTLPAS APKNGHYKAY ILGEGPDGVA
    360 370 380 390 400
    KTPEWASQIT GVPADKIIKL AREIGSTKPA FISQGWGPQR HANGEIATRA
    410 420 430 440 450
    ISMLAILTGN VGINGGNSGA REGSYSLPFV RMPTLENPIQ TSISMFMWTD
    460 470 480 490 500
    AIERGPEMTA LRDGVRGKDK LDVPIKMIWN YAGNCLINQH SEINRTHEIL
    510 520 530 540 550
    QDDKKCELIV VIDCHMTSSA KYADILLPDC TASEQMDFAL DASCGNMSYV
    560 570 580 590 600
    IFNDQVIKPR FECKTIYEMT SELAKRLGVE QQFTEGRTQE EWMRHLYAQS
    610 620 630 640 650
    REAIPELPTF EEFRKQGIFK KRDPQGHHVA YKAFREDPQA NPLTTPSGKI
    660 670 680 690 700
    EIYSQALADI AATWELPEGD VIDPLPIYTP GFESYQDPLN KQYPLQLTGF
    710 720 730 740 750
    HYKSRVHSTY GNVDVLKAAC RQEMWINPLD AQKRGIHNGD KVRIFNDRGE
    760 770 780 790 800
    VHIEAKVTPR MMPGVVALGE GAWYDPDAKR VDKGGCINVL TTQRPSPLAK
    810
    GNPSHTNLVQ VEKV
    Length:814
    Mass (Da):90,399
    Last modified:December 1, 2000 - v2
    Checksum:iB97C830ABAC7C32C
    GO

    Sequence cautioni

    The sequence AAA83843 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J03412 Genomic DNA Translation: AAA83843.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73980.2
    AP009048 Genomic DNA Translation: BAA35626.2
    PIRiS03785
    RefSeqiNP_415414.4, NC_000913.3
    WP_000850303.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73980; AAC73980; b0894
    BAA35626; BAA35626; BAA35626
    GeneIDi945508
    KEGGiecj:JW5118
    eco:b0894
    PATRICifig|1411691.4.peg.1383

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    J03412 Genomic DNA Translation: AAA83843.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73980.2
    AP009048 Genomic DNA Translation: BAA35626.2
    PIRiS03785
    RefSeqiNP_415414.4, NC_000913.3
    WP_000850303.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP18775
    SMRiP18775
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261945, 69 interactors
    ComplexPortaliCPX-320 DMSO reductase complex
    DIPiDIP-9452N
    IntActiP18775, 8 interactors
    MINTiP18775
    STRINGi316385.ECDH10B_0964

    Protein family/group databases

    TCDBi5.A.3.3.2 the prokaryotic molybdopterin-containing oxidoreductase (pmo) family

    Proteomic databases

    PaxDbiP18775
    PRIDEiP18775

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73980; AAC73980; b0894
    BAA35626; BAA35626; BAA35626
    GeneIDi945508
    KEGGiecj:JW5118
    eco:b0894
    PATRICifig|1411691.4.peg.1383

    Organism-specific databases

    EchoBASEiEB0228
    EcoGeneiEG10232 dmsA

    Phylogenomic databases

    eggNOGiENOG4107QY8 Bacteria
    COG0243 LUCA
    HOGENOMiHOG000284390
    InParanoidiP18775
    KOiK07306
    PhylomeDBiP18775

    Enzyme and pathway databases

    BioCyciEcoCyc:DMSA-MONOMER
    MetaCyc:DMSA-MONOMER
    BRENDAi1.8.5.3 2026

    Miscellaneous databases

    PROiPR:P18775

    Family and domain databases

    InterProiView protein in InterPro
    IPR011888 Anaer_DMSO_reductase
    IPR009010 Asp_de-COase-like_dom_sf
    IPR006657 MoPterin_dinucl-bd_dom
    IPR006656 Mopterin_OxRdtase
    IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
    IPR006655 Mopterin_OxRdtase_prok_CS
    IPR027467 MopterinOxRdtase_cofactor_BS
    IPR006311 TAT_signal
    IPR019546 TAT_signal_bac_arc
    PfamiView protein in Pfam
    PF04879 Molybdop_Fe4S4, 1 hit
    PF00384 Molybdopterin, 1 hit
    PF01568 Molydop_binding, 1 hit
    SMARTiView protein in SMART
    SM00926 Molybdop_Fe4S4, 1 hit
    SUPFAMiSSF50692 SSF50692, 1 hit
    TIGRFAMsiTIGR02166 dmsA_ynfE, 1 hit
    TIGR01409 TAT_signal_seq, 1 hit
    PROSITEiView protein in PROSITE
    PS51669 4FE4S_MOW_BIS_MGD, 1 hit
    PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
    PS00490 MOLYBDOPTERIN_PROK_2, 1 hit
    PS00932 MOLYBDOPTERIN_PROK_3, 1 hit
    PS51318 TAT, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDMSA_ECOLI
    AccessioniPrimary (citable) accession number: P18775
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1990
    Last sequence update: December 1, 2000
    Last modified: November 7, 2018
    This is version 169 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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