UniProtKB - P18762 (ADRB2_MOUSE)
Beta-2 adrenergic receptor
Adrb2
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 113 | Agonist or antagonistBy similarity | 1 | |
Binding sitei | 118 | Agonist or antagonistBy similarity | 1 |
GO - Molecular functioni
- adenylate cyclase binding Source: BHF-UCL
- amyloid-beta binding Source: MGI
- B2 bradykinin receptor binding Source: MGI
- beta2-adrenergic receptor activity Source: MGI
- drug binding Source: MGI
- enzyme binding Source: BHF-UCL
- G-protein alpha-subunit binding Source: MGI
- G protein-coupled receptor activity Source: GO_Central
- identical protein binding Source: MGI
- ionotropic glutamate receptor binding Source: MGI
- norepinephrine binding Source: HGNC-UCL
- potassium channel regulator activity Source: MGI
- protein-containing complex binding Source: MGI
- protein homodimerization activity Source: HGNC-UCL
- protein phosphatase 2A binding Source: MGI
- receptor-receptor interaction Source: MGI
GO - Biological processi
- activation of adenylate cyclase activity Source: HGNC-UCL
- adenylate cyclase-activating adrenergic receptor signaling pathway Source: GO_Central
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: MGI
- adrenergic receptor signaling pathway Source: MGI
- associative learning Source: MGI
- bone resorption Source: MGI
- brown fat cell differentiation Source: MGI
- cellular response to amyloid-beta Source: ARUK-UCL
- cellular response to hypoxia Source: MGI
- desensitization of G protein-coupled receptor signaling pathway by arrestin Source: HGNC-UCL
- diaphragm contraction Source: MGI
- diet induced thermogenesis Source: MGI
- excitatory postsynaptic potential Source: MGI
- G protein-coupled receptor signaling pathway Source: MGI
- heat generation Source: MGI
- negative regulation of angiogenesis Source: MGI
- negative regulation of inflammatory response Source: MGI
- negative regulation of multicellular organism growth Source: MGI
- negative regulation of ossification Source: MGI
- negative regulation of platelet aggregation Source: MGI
- negative regulation of smooth muscle contraction Source: MGI
- negative regulation of urine volume Source: MGI
- norepinephrine-epinephrine-mediated vasodilation involved in regulation of systemic arterial blood pressure Source: MGI
- positive regulation of AMPA receptor activity Source: MGI
- positive regulation of apoptotic process Source: MGI
- positive regulation of ATPase activity Source: MGI
- positive regulation of autophagosome maturation Source: GO_Central
- positive regulation of blood vessel diameter Source: MGI
- positive regulation of bone mineralization Source: MGI
- positive regulation of calcium ion import across plasma membrane Source: MGI
- positive regulation of cell population proliferation Source: MGI
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of heart contraction Source: MGI
- positive regulation of lipophagy Source: GO_Central
- positive regulation of MAPK cascade Source: HGNC-UCL
- positive regulation of mini excitatory postsynaptic potential Source: ARUK-UCL
- positive regulation of potassium ion transport Source: MGI
- positive regulation of protein kinase A signaling Source: ARUK-UCL
- positive regulation of protein serine/threonine kinase activity Source: MGI
- positive regulation of protein ubiquitination Source: MGI
- positive regulation of skeletal muscle tissue growth Source: MGI
- positive regulation of sodium ion transport Source: MGI
- positive regulation of the force of heart contraction by epinephrine Source: MGI
- positive regulation of transcription by RNA polymerase II Source: MGI
- receptor-mediated endocytosis Source: HGNC-UCL
- regulation of calcium ion transport Source: MGI
- regulation of sensory perception of pain Source: MGI
- regulation of sodium ion transport Source: MGI
- regulation of synaptic vesicle exocytosis Source: MGI
- regulation of systemic arterial blood pressure by norepinephrine-epinephrine Source: MGI
- response to cold Source: MGI
- synaptic transmission, glutamatergic Source: MGI
- wound healing Source: MGI
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Enzyme and pathway databases
Reactomei | R-MMU-390696, Adrenoceptors R-MMU-418555, G alpha (s) signalling events R-MMU-5689880, Ub-specific processing proteases R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-2 adrenergic receptorAlternative name(s): Beta-2 adrenoreceptor Short name: Beta-2 adrenoceptor |
Gene namesi | Name:Adrb2 Synonyms:Adrb2r |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:87938, Adrb2 |
Subcellular locationi
Endosome
- Early endosome By similarity
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein By similarity
Golgi apparatus
- Golgi apparatus By similarity
Note: Colocalizes with VHL at the cell membrane. Activated receptors are internalized into endosomes prior to their degradation in lysosomes. Activated receptors are also detected within the Golgi apparatus.By similarity
Endosome
- early endosome Source: UniProtKB-SubCell
- endosome Source: MGI
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Nucleus
- nucleus Source: MGI
Plasma Membrane
- apical plasma membrane Source: MGI
- caveola Source: MGI
- integral component of plasma membrane Source: MGI
- neuronal cell body membrane Source: MGI
- plasma membrane Source: MGI
- sarcolemma Source: MGI
Other locations
- axon Source: MGI
- cytoplasm Source: MGI
- dendrite Source: MGI
- dendritic spine Source: MGI
- glutamatergic synapse Source: MGI
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: MGI
- receptor complex Source: HGNC-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 34 | ExtracellularBy similarityAdd BLAST | 34 | |
Transmembranei | 35 – 58 | Helical; Name=1By similarityAdd BLAST | 24 | |
Topological domaini | 59 – 71 | CytoplasmicBy similarityAdd BLAST | 13 | |
Transmembranei | 72 – 95 | Helical; Name=2By similarityAdd BLAST | 24 | |
Topological domaini | 96 – 106 | ExtracellularBy similarityAdd BLAST | 11 | |
Transmembranei | 107 – 129 | Helical; Name=3By similarityAdd BLAST | 23 | |
Topological domaini | 130 – 150 | CytoplasmicBy similarityAdd BLAST | 21 | |
Transmembranei | 151 – 174 | Helical; Name=4By similarityAdd BLAST | 24 | |
Topological domaini | 175 – 196 | ExtracellularBy similarityAdd BLAST | 22 | |
Transmembranei | 197 – 220 | Helical; Name=5By similarityAdd BLAST | 24 | |
Topological domaini | 221 – 274 | CytoplasmicBy similarityAdd BLAST | 54 | |
Transmembranei | 275 – 298 | Helical; Name=6By similarityAdd BLAST | 24 | |
Topological domaini | 299 – 305 | ExtracellularBy similarity | 7 | |
Transmembranei | 306 – 329 | Helical; Name=7By similarityAdd BLAST | 24 | |
Topological domaini | 330 – 418 | CytoplasmicBy similarityAdd BLAST | 89 |
Keywords - Cellular componenti
Cell membrane, Endosome, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000069134 | 1 – 418 | Beta-2 adrenergic receptorAdd BLAST | 418 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 6 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Glycosylationi | 15 | N-linked (GlcNAc...) asparagineCurated | 1 | |
Disulfide bondi | 106 ↔ 191 | PROSITE-ProRule annotation | ||
Modified residuei | 141 | PhosphotyrosineBy similarity | 1 | |
Disulfide bondi | 184 ↔ 190 | PROSITE-ProRule annotation | ||
Modified residuei | 246 | PhosphoserineBy similarity | 1 | |
Modified residuei | 261 | Phosphoserine; by PKASequence analysis | 1 | |
Modified residuei | 262 | Phosphoserine; by PKASequence analysis | 1 | |
Lipidationi | 265 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 341 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 345 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 346 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 355 | Phosphoserine; by BARKCurated | 1 | |
Modified residuei | 356 | Phosphoserine; by BARKCurated | 1 | |
Modified residuei | 387 | 4-hydroxyprolineBy similarity | 1 | |
Modified residuei | 400 | 4-hydroxyprolineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Hydroxylation, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | P18762 |
PaxDbi | P18762 |
PRIDEi | P18762 |
PTM databases
GlyGeni | P18762, 2 sites |
iPTMneti | P18762 |
PhosphoSitePlusi | P18762 |
SwissPalmi | P18762 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000045730, Expressed in granulocyte and 180 other tissues |
Genevisiblei | P18762, MM |
Interactioni
Subunit structurei
Binds SLC9A3R1 and GPRASP1.
Interacts with ARRB1 and ARRB2.
Interacts with SRC, USP20 and USP33.
Interacts with VHL; the interaction, which is increased on hydroxylation of ADRB2, ubiquitinates ADRB2 leading to its degradation.
Interacts with EGLN3; the interaction hydroxylates ADRB2 facilitating VHL-E3 ligase-mediated ubiquitination.
Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required when endocytosed to prevent degradation in lysosomes and promote recycling to the plasma membrane.
Interacts with CNIH4.
Interacts with ARRDC3.
By similarityBinary interactionsi
Hide detailsP18762
With | #Exp. | IntAct |
---|---|---|
Adrb1 [P34971] | 2 | EBI-491143,EBI-7764182 |
Cav3 [P51637] | 2 | EBI-491143,EBI-298576 |
Erbb2 [P70424] | 3 | EBI-491143,EBI-2945468 |
GO - Molecular functioni
- adenylate cyclase binding Source: BHF-UCL
- B2 bradykinin receptor binding Source: MGI
- enzyme binding Source: BHF-UCL
- G-protein alpha-subunit binding Source: MGI
- identical protein binding Source: MGI
- ionotropic glutamate receptor binding Source: MGI
- protein homodimerization activity Source: HGNC-UCL
- protein phosphatase 2A binding Source: MGI
- receptor-receptor interaction Source: MGI
Protein-protein interaction databases
CORUMi | P18762 |
DIPi | DIP-59568N |
IntActi | P18762, 9 interactors |
MINTi | P18762 |
STRINGi | 10090.ENSMUSP00000062256 |
Chemistry databases
BindingDBi | P18762 |
Miscellaneous databases
RNActi | P18762, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 193 – 207 | Agonist and antagonist bindingBy similarityAdd BLAST | 15 | |
Regioni | 286 – 293 | Agonist and antagonist bindingBy similarity | 8 | |
Regioni | 312 – 316 | Agonist and antagonist bindingBy similarity | 5 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 415 – 418 | PDZ-binding | 4 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000159538 |
HOGENOMi | CLU_009579_11_0_1 |
InParanoidi | P18762 |
OMAi | CLREHKA |
OrthoDBi | 614199at2759 |
TreeFami | TF316350 |
Family and domain databases
InterProi | View protein in InterPro IPR002233, ADR_fam IPR000332, ADRB2_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24248:SF21, PTHR24248:SF21, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01103, ADRENERGICR PR00562, ADRENRGCB2AR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGPHGNDSDF LLAPNGSRAP DHDVTQERDE AWVVGMAILM SVIVLAIVFG
60 70 80 90 100
NVLVITAIAK FERLQTVTNY FIISLACADL VMGLAVVPFG ASHILMKMWN
110 120 130 140 150
FGNFWCEFWT SIDVLCVTAS IETLCVIAVD RYVAITSPFK YQSLLTKNKA
160 170 180 190 200
RVVILMVWIV SGLTSFLPIQ MHWYRATHKK AIDCYTEETC CDFFTNQAYA
210 220 230 240 250
IASSIVSFYV PLVVMVFVYS RVFQVAKRQL QKIDKSEGRF HAQNLSQVEQ
260 270 280 290 300
DGRSGHGLRR SSKFCLKEHK ALKTLGIIMG TFTLCWLPFF IVNIVHVIRD
310 320 330 340 350
NLIPKEVYIL LNWLGYVNSA FNPLIYCRSP DFRIAFQELL CLRRSSSKTY
360 370 380 390 400
GNGYSSNSNG RTDYTGEPNT CQLGQEREQE LLCEDPPGME GFVNCQGTVP
410
SLSVDSQGRN CSTNDSPL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 21 | D → H in CAA33664 (PubMed:2549959).Curated | 1 | |
Sequence conflicti | 94 – 95 | IL → TS in CAA33664 (PubMed:2549959).Curated | 2 | |
Sequence conflicti | 254 | S → T in CAA33664 (PubMed:2549959).Curated | 1 | |
Sequence conflicti | 347 – 348 | SK → FE in CAA33664 (PubMed:2549959).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15643 Genomic DNA Translation: CAA33664.1 AK080241 mRNA Translation: BAC37856.1 CH466528 Genomic DNA Translation: EDL09746.1 BC032883 mRNA Translation: AAH32883.1 |
CCDSi | CCDS29289.1 |
PIRi | S00260 |
RefSeqi | NP_031446.2, NM_007420.3 |
Genome annotation databases
Ensembli | ENSMUST00000053640; ENSMUSP00000062256; ENSMUSG00000045730 |
GeneIDi | 11555 |
KEGGi | mmu:11555 |
UCSCi | uc008fcy.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X15643 Genomic DNA Translation: CAA33664.1 AK080241 mRNA Translation: BAC37856.1 CH466528 Genomic DNA Translation: EDL09746.1 BC032883 mRNA Translation: AAH32883.1 |
CCDSi | CCDS29289.1 |
PIRi | S00260 |
RefSeqi | NP_031446.2, NM_007420.3 |
3D structure databases
SMRi | P18762 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | P18762 |
DIPi | DIP-59568N |
IntActi | P18762, 9 interactors |
MINTi | P18762 |
STRINGi | 10090.ENSMUSP00000062256 |
Chemistry databases
BindingDBi | P18762 |
ChEMBLi | CHEMBL3707 |
DrugCentrali | P18762 |
Protein family/group databases
GPCRDBi | Search... |
PTM databases
GlyGeni | P18762, 2 sites |
iPTMneti | P18762 |
PhosphoSitePlusi | P18762 |
SwissPalmi | P18762 |
Proteomic databases
jPOSTi | P18762 |
PaxDbi | P18762 |
PRIDEi | P18762 |
Protocols and materials databases
Antibodypediai | 15959, 1068 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000053640; ENSMUSP00000062256; ENSMUSG00000045730 |
GeneIDi | 11555 |
KEGGi | mmu:11555 |
UCSCi | uc008fcy.2, mouse |
Organism-specific databases
CTDi | 154 |
MGIi | MGI:87938, Adrb2 |
Phylogenomic databases
eggNOGi | KOG3656, Eukaryota |
GeneTreei | ENSGT00940000159538 |
HOGENOMi | CLU_009579_11_0_1 |
InParanoidi | P18762 |
OMAi | CLREHKA |
OrthoDBi | 614199at2759 |
TreeFami | TF316350 |
Enzyme and pathway databases
Reactomei | R-MMU-390696, Adrenoceptors R-MMU-418555, G alpha (s) signalling events R-MMU-5689880, Ub-specific processing proteases R-MMU-8856825, Cargo recognition for clathrin-mediated endocytosis R-MMU-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 11555, 1 hit in 17 CRISPR screens |
ChiTaRSi | Adrb2, mouse |
PROi | PR:P18762 |
RNActi | P18762, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000045730, Expressed in granulocyte and 180 other tissues |
Genevisiblei | P18762, MM |
Family and domain databases
InterProi | View protein in InterPro IPR002233, ADR_fam IPR000332, ADRB2_rcpt IPR000276, GPCR_Rhodpsn IPR017452, GPCR_Rhodpsn_7TM |
PANTHERi | PTHR24248:SF21, PTHR24248:SF21, 1 hit |
Pfami | View protein in Pfam PF00001, 7tm_1, 1 hit |
PRINTSi | PR01103, ADRENERGICR PR00562, ADRENRGCB2AR PR00237, GPCRRHODOPSN |
SMARTi | View protein in SMART SM01381, 7TM_GPCR_Srsx, 1 hit |
PROSITEi | View protein in PROSITE PS00237, G_PROTEIN_RECEP_F1_1, 1 hit PS50262, G_PROTEIN_RECEP_F1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADRB2_MOUSE | |
Accessioni | P18762Primary (citable) accession number: P18762 Secondary accession number(s): Q8BH38 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1990 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 197 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - 7-transmembrane G-linked receptors
List of 7-transmembrane G-linked receptor entries